IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
cDNA cloning and expression of Acyl-CoA binding protein (ACBP) from orange-spotted grouper, Epinephelus coioides
Citarasu, Thavasimuthu1,2; Zhou, Li2; Yang, Wang2; Gui, Jian Fang2; Citarasu, T (reprint author), Manonmaniam Sundaranar Univ, Ctr Marine Sci & Technol, Rajakkamangalam 629502, Kanyakumari, India.
2013-07-01
Source PublicationINDIAN JOURNAL OF BIOTECHNOLOGY
ISSN0972-5849
Volume12Issue:3Pages:330-335
AbstractcDNA of acyl-CoA binding protein (ACBP) was constructed, cloned and expressed in hypothalamus and pituitary of orange-spotted grouper, Epinephelus coioides. The ACBP gene contains 630 nucleotides including the open reading frame (ORE) of length 128 bp. The open reading frame was started with ATG codon at the position 462 and terminated with a stop codon TAA at the position 600. The ORE sequence consisted of 42 amino acids and the calculated mol wt of the protein was approx 4.62 kDa (GenBank: FJ939282.1). The highest identity was observed with the puffer fish, Tetraodon nigroviridis and Anoplopoma fimbria with homology of 83 and 88.1%, respectively. RT-PCR results revealed that ACBP mRNA was abundantly expressed in pituitary, hypothalamus and medulla oblongata. Further, ACBP was cloned in pGEX-KG expression vector and transformed into BL21(DE3). The protein was purified by affinity chromatography and partially characterized. The abundant expression of ACBP in pituitary and hypothalamus, in the present study, indicated that it might be involved in reproduction.; cDNA of acyl-CoA binding protein (ACBP) was constructed, cloned and expressed in hypothalamus and pituitary of orange-spotted grouper, Epinephelus coioides. The ACBP gene contains 630 nucleotides including the open reading frame (ORE) of length 128 bp. The open reading frame was started with ATG codon at the position 462 and terminated with a stop codon TAA at the position 600. The ORE sequence consisted of 42 amino acids and the calculated mol wt of the protein was approx 4.62 kDa (GenBank: FJ939282.1). The highest identity was observed with the puffer fish, Tetraodon nigroviridis and Anoplopoma fimbria with homology of 83 and 88.1%, respectively. RT-PCR results revealed that ACBP mRNA was abundantly expressed in pituitary, hypothalamus and medulla oblongata. Further, ACBP was cloned in pGEX-KG expression vector and transformed into BL21(DE3). The protein was purified by affinity chromatography and partially characterized. The abundant expression of ACBP in pituitary and hypothalamus, in the present study, indicated that it might be involved in reproduction.
SubtypeArticle
KeywordAcyl-co a Binding Protein (Acbp) Cdna Cloning Epinephelus Coioides
Department[Citarasu, Thavasimuthu] Manonmaniam Sundaranar Univ, Ctr Marine Sci & Technol, Rajakkamangalam 629502, Kanyakumari, India ; [Citarasu, Thavasimuthu ; Zhou, Li ; Yang, Wang ; Gui, Jian Fang] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationCAS-TWAS, Chinese Academy of Sciences, Beijing, China; Third World Academy of Sciences, Trieste, Italy ; CAS-TWAS, Chinese Academy of Sciences, Beijing, China; Third World Academy of Sciences, Trieste, Italy ; CAS-TWAS, Chinese Academy of Sciences, Beijing, China; Third World Academy of Sciences, Trieste, Italy ; CAS-TWAS, Chinese Academy of Sciences, Beijing, China; Third World Academy of Sciences, Trieste, Italy
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000327558100004
WOS KeywordRAT-BRAIN ; INHIBITOR ; ENDOZEPINE ; COENZYME ; DBI
Funding OrganizationCAS-TWAS, Chinese Academy of Sciences, Beijing, China; Third World Academy of Sciences, Trieste, Italy ; CAS-TWAS, Chinese Academy of Sciences, Beijing, China; Third World Academy of Sciences, Trieste, Italy ; CAS-TWAS, Chinese Academy of Sciences, Beijing, China; Third World Academy of Sciences, Trieste, Italy ; CAS-TWAS, Chinese Academy of Sciences, Beijing, China; Third World Academy of Sciences, Trieste, Italy
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19778
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorCitarasu, T (reprint author), Manonmaniam Sundaranar Univ, Ctr Marine Sci & Technol, Rajakkamangalam 629502, Kanyakumari, India.
Affiliation1.Manonmaniam Sundaranar Univ, Ctr Marine Sci & Technol, Rajakkamangalam 629502, Kanyakumari, India
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Citarasu, Thavasimuthu,Zhou, Li,Yang, Wang,et al. cDNA cloning and expression of Acyl-CoA binding protein (ACBP) from orange-spotted grouper, Epinephelus coioides[J]. INDIAN JOURNAL OF BIOTECHNOLOGY,2013,12(3):330-335.
APA Citarasu, Thavasimuthu,Zhou, Li,Yang, Wang,Gui, Jian Fang,&Citarasu, T .(2013).cDNA cloning and expression of Acyl-CoA binding protein (ACBP) from orange-spotted grouper, Epinephelus coioides.INDIAN JOURNAL OF BIOTECHNOLOGY,12(3),330-335.
MLA Citarasu, Thavasimuthu,et al."cDNA cloning and expression of Acyl-CoA binding protein (ACBP) from orange-spotted grouper, Epinephelus coioides".INDIAN JOURNAL OF BIOTECHNOLOGY 12.3(2013):330-335.
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