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题名: cDNA cloning and expression of Acyl-CoA binding protein (ACBP) from orange-spotted grouper, Epinephelus coioides
作者: Citarasu, Thavasimuthu1, 2; Zhou, Li2; Yang, Wang2; Gui, Jian Fang2
通讯作者: Citarasu, T (reprint author), Manonmaniam Sundaranar Univ, Ctr Marine Sci & Technol, Rajakkamangalam 629502, Kanyakumari, India.
关键词: Acyl-Co A binding protein (ACBP) ; cDNA cloning ; Epinephelus coioides
刊名: INDIAN JOURNAL OF BIOTECHNOLOGY
发表日期: 2013-07-01
卷: 12, 期:3, 页:330-335
收录类别: SCI
文章类型: Article
部门归属: [Citarasu, Thavasimuthu] Manonmaniam Sundaranar Univ, Ctr Marine Sci & Technol, Rajakkamangalam 629502, Kanyakumari, India ; [Citarasu, Thavasimuthu ; Zhou, Li ; Yang, Wang ; Gui, Jian Fang] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: CAS-TWAS, Chinese Academy of Sciences, Beijing, China; Third World Academy of Sciences, Trieste, Italy
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
摘要: cDNA of acyl-CoA binding protein (ACBP) was constructed, cloned and expressed in hypothalamus and pituitary of orange-spotted grouper, Epinephelus coioides. The ACBP gene contains 630 nucleotides including the open reading frame (ORE) of length 128 bp. The open reading frame was started with ATG codon at the position 462 and terminated with a stop codon TAA at the position 600. The ORE sequence consisted of 42 amino acids and the calculated mol wt of the protein was approx 4.62 kDa (GenBank: FJ939282.1). The highest identity was observed with the puffer fish, Tetraodon nigroviridis and Anoplopoma fimbria with homology of 83 and 88.1%, respectively. RT-PCR results revealed that ACBP mRNA was abundantly expressed in pituitary, hypothalamus and medulla oblongata. Further, ACBP was cloned in pGEX-KG expression vector and transformed into BL21(DE3). The protein was purified by affinity chromatography and partially characterized. The abundant expression of ACBP in pituitary and hypothalamus, in the present study, indicated that it might be involved in reproduction.
英文摘要: cDNA of acyl-CoA binding protein (ACBP) was constructed, cloned and expressed in hypothalamus and pituitary of orange-spotted grouper, Epinephelus coioides. The ACBP gene contains 630 nucleotides including the open reading frame (ORE) of length 128 bp. The open reading frame was started with ATG codon at the position 462 and terminated with a stop codon TAA at the position 600. The ORE sequence consisted of 42 amino acids and the calculated mol wt of the protein was approx 4.62 kDa (GenBank: FJ939282.1). The highest identity was observed with the puffer fish, Tetraodon nigroviridis and Anoplopoma fimbria with homology of 83 and 88.1%, respectively. RT-PCR results revealed that ACBP mRNA was abundantly expressed in pituitary, hypothalamus and medulla oblongata. Further, ACBP was cloned in pGEX-KG expression vector and transformed into BL21(DE3). The protein was purified by affinity chromatography and partially characterized. The abundant expression of ACBP in pituitary and hypothalamus, in the present study, indicated that it might be involved in reproduction.
关键词[WOS]: RAT-BRAIN ; INHIBITOR ; ENDOZEPINE ; COENZYME ; DBI
语种: 英语
项目资助者: CAS-TWAS, Chinese Academy of Sciences, Beijing, China ; Third World Academy of Sciences, Trieste, Italy
WOS记录号: WOS:000327558100004
ISSN号: 0972-5849
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19778
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Manonmaniam Sundaranar Univ, Ctr Marine Sci & Technol, Rajakkamangalam 629502, Kanyakumari, India
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

Recommended Citation:
Citarasu, Thavasimuthu; Zhou, Li; Yang, Wang; Gui, Jian Fang.cDNA cloning and expression of Acyl-CoA binding protein (ACBP) from orange-spotted grouper, Epinephelus coioides,INDIAN JOURNAL OF BIOTECHNOLOGY,2013,12(3):330-335
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