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Increased food intake in growth hormone-transgenic common carp (Cyprinus carpio L.) may be mediated by upregulating Agouti-related protein (AgRP)
Zhong, Chengrong1,2; Song, Yanlong1,2; Wang, Yaping1; Zhang, Tanglin1; Duan, Ming1; Li, Yongming1; Liao, Lanjie1; Zhu, Zuoyan1; Hu, Wei1; Hu, W (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
2013-10-01
Source PublicationGENERAL AND COMPARATIVE ENDOCRINOLOGY
ISSN0016-6480
Volume192Pages:81-88
AbstractIn fish, food intake and feeding behavior are crucial for survival, competition, growth and reproduction. Growth hormone (GH)-transgenic common carp exhibit an enhanced growth rate, increased food intake and higher feed conversion rate. However, the underlying molecular mechanisms of feeding regulation in GH-transgenic (TG) fish are not clear. In this study, we observed feeding behavior of TG and non-transgenic (NT) common carp, and analyzed the mRNA expression levels of NPY, AgRP I, orexin, POMC, CCK, and CART I in the hypothalamus and telencephalon after behavioral observation. We detected similar gene expression levels in the hypothalamus of TG and NT common carp, which had been cultured in the field at the same age. Furthermore, we tested the effects of GH on hypothalamus fragments in vitro to confirm our findings. We demonstrated that TG common carp displayed increased food intake and reduced food consumption time, which were associated with a marked increase in hypothalamic AgRP I mRNA expression. Our results suggest that elevated GH levels may influence food intake and feeding behavior by upregulating the hypothalamic orexigenic factor AgRP I in GH-transgenic common carp. (C) 2013 Elsevier Inc. All rights reserved.; In fish, food intake and feeding behavior are crucial for survival, competition, growth and reproduction. Growth hormone (GH)-transgenic common carp exhibit an enhanced growth rate, increased food intake and higher feed conversion rate. However, the underlying molecular mechanisms of feeding regulation in GH-transgenic (TG) fish are not clear. In this study, we observed feeding behavior of TG and non-transgenic (NT) common carp, and analyzed the mRNA expression levels of NPY, AgRP I, orexin, POMC, CCK, and CART I in the hypothalamus and telencephalon after behavioral observation. We detected similar gene expression levels in the hypothalamus of TG and NT common carp, which had been cultured in the field at the same age. Furthermore, we tested the effects of GH on hypothalamus fragments in vitro to confirm our findings. We demonstrated that TG common carp displayed increased food intake and reduced food consumption time, which were associated with a marked increase in hypothalamic AgRP I mRNA expression. Our results suggest that elevated GH levels may influence food intake and feeding behavior by upregulating the hypothalamic orexigenic factor AgRP I in GH-transgenic common carp. (C) 2013 Elsevier Inc. All rights reserved.
SubtypeArticle
KeywordGrowth Hormone Agrp Npy Food Intake Transgenic Common Carp
Department[Zhong, Chengrong ; Song, Yanlong ; Wang, Yaping ; Zhang, Tanglin ; Duan, Ming ; Li, Yongming ; Liao, Lanjie ; Zhu, Zuoyan ; Hu, Wei] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Zhong, Chengrong ; Song, Yanlong] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
DOI10.1016/j.ygcen.2013.03.024
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation [30930069]; Development Plan of the State Key Fundamental Research of China [2010CB126302]; Key Research Program of the Chinese Academy of Sciences [KSCX2-EW-N-004]; '863' High Technology Project [2011AA100404] ; National Natural Science Foundation [30930069]; Development Plan of the State Key Fundamental Research of China [2010CB126302]; Key Research Program of the Chinese Academy of Sciences [KSCX2-EW-N-004]; '863' High Technology Project [2011AA100404] ; National Natural Science Foundation [30930069]; Development Plan of the State Key Fundamental Research of China [2010CB126302]; Key Research Program of the Chinese Academy of Sciences [KSCX2-EW-N-004]; '863' High Technology Project [2011AA100404] ; National Natural Science Foundation [30930069]; Development Plan of the State Key Fundamental Research of China [2010CB126302]; Key Research Program of the Chinese Academy of Sciences [KSCX2-EW-N-004]; '863' High Technology Project [2011AA100404]
Indexed BySCI
Language英语
WOS Research AreaEndocrinology & Metabolism
WOS SubjectEndocrinology & Metabolism
WOS IDWOS:000325736400011
WOS KeywordSALMON ONCORHYNCHUS-KISUTCH ; NEUROPEPTIDE-Y NEURONS ; CCK/GASTRIN-LIKE IMMUNOREACTIVITY ; COHO SALMON ; ATLANTIC SALMON ; CARASSIUS-AURATUS ; FEEDING-BEHAVIOR ; MELANOCORTIN-4 RECEPTOR ; SWIMMING PERFORMANCE ; HYPOTHALAMIC ARCUATE
Funding OrganizationNational Natural Science Foundation [30930069]; Development Plan of the State Key Fundamental Research of China [2010CB126302]; Key Research Program of the Chinese Academy of Sciences [KSCX2-EW-N-004]; '863' High Technology Project [2011AA100404] ; National Natural Science Foundation [30930069]; Development Plan of the State Key Fundamental Research of China [2010CB126302]; Key Research Program of the Chinese Academy of Sciences [KSCX2-EW-N-004]; '863' High Technology Project [2011AA100404] ; National Natural Science Foundation [30930069]; Development Plan of the State Key Fundamental Research of China [2010CB126302]; Key Research Program of the Chinese Academy of Sciences [KSCX2-EW-N-004]; '863' High Technology Project [2011AA100404] ; National Natural Science Foundation [30930069]; Development Plan of the State Key Fundamental Research of China [2010CB126302]; Key Research Program of the Chinese Academy of Sciences [KSCX2-EW-N-004]; '863' High Technology Project [2011AA100404]
Citation statistics
Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19776
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorHu, W (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zhong, Chengrong,Song, Yanlong,Wang, Yaping,et al. Increased food intake in growth hormone-transgenic common carp (Cyprinus carpio L.) may be mediated by upregulating Agouti-related protein (AgRP)[J]. GENERAL AND COMPARATIVE ENDOCRINOLOGY,2013,192:81-88.
APA Zhong, Chengrong.,Song, Yanlong.,Wang, Yaping.,Zhang, Tanglin.,Duan, Ming.,...&Hu, W .(2013).Increased food intake in growth hormone-transgenic common carp (Cyprinus carpio L.) may be mediated by upregulating Agouti-related protein (AgRP).GENERAL AND COMPARATIVE ENDOCRINOLOGY,192,81-88.
MLA Zhong, Chengrong,et al."Increased food intake in growth hormone-transgenic common carp (Cyprinus carpio L.) may be mediated by upregulating Agouti-related protein (AgRP)".GENERAL AND COMPARATIVE ENDOCRINOLOGY 192(2013):81-88.
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