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题名: Increased food intake in growth hormone-transgenic common carp (Cyprinus carpio L.) may be mediated by upregulating Agouti-related protein (AgRP)
作者: Zhong, Chengrong1, 2; Song, Yanlong1, 2; Wang, Yaping1; Zhang, Tanglin1; Duan, Ming1; Li, Yongming1; Liao, Lanjie1; Zhu, Zuoyan1; Hu, Wei1
通讯作者: Hu, W (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
关键词: Growth hormone ; AgRP ; NPY ; Food intake ; Transgenic ; Common carp
刊名: GENERAL AND COMPARATIVE ENDOCRINOLOGY
发表日期: 2013-10-01
DOI: 10.1016/j.ygcen.2013.03.024
卷: 192, 页:81-88
收录类别: SCI
文章类型: Article
部门归属: [Zhong, Chengrong ; Song, Yanlong ; Wang, Yaping ; Zhang, Tanglin ; Duan, Ming ; Li, Yongming ; Liao, Lanjie ; Zhu, Zuoyan ; Hu, Wei] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Zhong, Chengrong ; Song, Yanlong] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation [30930069]; Development Plan of the State Key Fundamental Research of China [2010CB126302]; Key Research Program of the Chinese Academy of Sciences [KSCX2-EW-N-004]; '863' High Technology Project [2011AA100404]
类目[WOS]: Endocrinology & Metabolism
研究领域[WOS]: Endocrinology & Metabolism
摘要: In fish, food intake and feeding behavior are crucial for survival, competition, growth and reproduction. Growth hormone (GH)-transgenic common carp exhibit an enhanced growth rate, increased food intake and higher feed conversion rate. However, the underlying molecular mechanisms of feeding regulation in GH-transgenic (TG) fish are not clear. In this study, we observed feeding behavior of TG and non-transgenic (NT) common carp, and analyzed the mRNA expression levels of NPY, AgRP I, orexin, POMC, CCK, and CART I in the hypothalamus and telencephalon after behavioral observation. We detected similar gene expression levels in the hypothalamus of TG and NT common carp, which had been cultured in the field at the same age. Furthermore, we tested the effects of GH on hypothalamus fragments in vitro to confirm our findings. We demonstrated that TG common carp displayed increased food intake and reduced food consumption time, which were associated with a marked increase in hypothalamic AgRP I mRNA expression. Our results suggest that elevated GH levels may influence food intake and feeding behavior by upregulating the hypothalamic orexigenic factor AgRP I in GH-transgenic common carp. (C) 2013 Elsevier Inc. All rights reserved.
英文摘要: In fish, food intake and feeding behavior are crucial for survival, competition, growth and reproduction. Growth hormone (GH)-transgenic common carp exhibit an enhanced growth rate, increased food intake and higher feed conversion rate. However, the underlying molecular mechanisms of feeding regulation in GH-transgenic (TG) fish are not clear. In this study, we observed feeding behavior of TG and non-transgenic (NT) common carp, and analyzed the mRNA expression levels of NPY, AgRP I, orexin, POMC, CCK, and CART I in the hypothalamus and telencephalon after behavioral observation. We detected similar gene expression levels in the hypothalamus of TG and NT common carp, which had been cultured in the field at the same age. Furthermore, we tested the effects of GH on hypothalamus fragments in vitro to confirm our findings. We demonstrated that TG common carp displayed increased food intake and reduced food consumption time, which were associated with a marked increase in hypothalamic AgRP I mRNA expression. Our results suggest that elevated GH levels may influence food intake and feeding behavior by upregulating the hypothalamic orexigenic factor AgRP I in GH-transgenic common carp. (C) 2013 Elsevier Inc. All rights reserved.
关键词[WOS]: SALMON ONCORHYNCHUS-KISUTCH ; NEUROPEPTIDE-Y NEURONS ; CCK/GASTRIN-LIKE IMMUNOREACTIVITY ; COHO SALMON ; ATLANTIC SALMON ; CARASSIUS-AURATUS ; FEEDING-BEHAVIOR ; MELANOCORTIN-4 RECEPTOR ; SWIMMING PERFORMANCE ; HYPOTHALAMIC ARCUATE
语种: 英语
WOS记录号: WOS:000325736400011
ISSN号: 0016-6480
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19776
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Zhong, Chengrong; Song, Yanlong; Wang, Yaping; Zhang, Tanglin; Duan, Ming; Li, Yongming; Liao, Lanjie; Zhu, Zuoyan; Hu, Wei.Increased food intake in growth hormone-transgenic common carp (Cyprinus carpio L.) may be mediated by upregulating Agouti-related protein (AgRP),GENERAL AND COMPARATIVE ENDOCRINOLOGY,2013,192():81-88
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