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题名: Oocyte-Specific H2A Variant H2af1o Is Required for Cell Synchrony Before Midblastula Transition in Early Zebrafish Embryos
作者: Yue, Hua-Mei1, 2; Li, Zhi1; Wu, Nan1; Liu, Zhen1; Wang, Yang1; Gui, Jian-Fang1
通讯作者: Gui, JF (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
关键词: cell synchrony ; early cleavage ; histone variant ; midblastula transition ; oocyte-specific histone
刊名: BIOLOGY OF REPRODUCTION
发表日期: 2013-10-01
DOI: 10.1095/biolreprod.113.108043
卷: 89, 期:4
收录类别: SCI
文章类型: Article
部门归属: [Yue, Hua-Mei ; Li, Zhi ; Wu, Nan ; Liu, Zhen ; Wang, Yang ; Gui, Jian-Fang] Chinese Acad Sci, Inst Hydrobiol, Grad Univ, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Yue, Hua-Mei] Chinese Acad Fisheries Sci, Key Lab Freshwater Biodivers Conservat, Minist Agr China, Yangtze River Fisheries Res Inst, Wuhan, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Key Basic Research Program of China [2010CB126301]; Special Fund for Agro-scientific Research in the Public Interest [200903046]; Chinese Academy of Sciences [KSCX3-EW-N-04]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17]
类目[WOS]: Reproductive Biology
研究领域[WOS]: Reproductive Biology
摘要: Oocyte-specific histone variants have been expected to play significant roles in early embryonic development, but the exact evidence and the biological function have remained unclear. Here, we present evidence that H2af1o, an oocyte-specific H2A variant, is required for cell synchrony before midblastula transition in early zebrafish embryos. The H2A variant is oocyte specific, peaks in mature eggs, and is supplied to early embryos. We constructed a series of deletion plasmids of the zebrafish h2af1o tagged with EGFP and determined the main key function regions including nuclear localization signal of N-terminal 25 amino acids and nucleosome binding region of 110-122 amino acid sequence in the C-terminus by microinjecting them into one-cell-stage zebrafish embryos. In comparison with ubiquitous H2A. X, the H2af1o was revealed to confer a more open structure than canonical H2A in the nucleosomes. Furthermore, we conducted the h2af1o-specific morpholino knockdown analysis in early embryos of zebrafish and revealed its biological function for maintaining cell synchrony division because the H2af1o deficiency disturbed cell synchrony in early cleavages before midblastula transition. Therefore, our current findings provided the first case to understand the biological function of maternal oocyte-specific histone variants in vertebrates.
英文摘要: Oocyte-specific histone variants have been expected to play significant roles in early embryonic development, but the exact evidence and the biological function have remained unclear. Here, we present evidence that H2af1o, an oocyte-specific H2A variant, is required for cell synchrony before midblastula transition in early zebrafish embryos. The H2A variant is oocyte specific, peaks in mature eggs, and is supplied to early embryos. We constructed a series of deletion plasmids of the zebrafish h2af1o tagged with EGFP and determined the main key function regions including nuclear localization signal of N-terminal 25 amino acids and nucleosome binding region of 110-122 amino acid sequence in the C-terminus by microinjecting them into one-cell-stage zebrafish embryos. In comparison with ubiquitous H2A. X, the H2af1o was revealed to confer a more open structure than canonical H2A in the nucleosomes. Furthermore, we conducted the h2af1o-specific morpholino knockdown analysis in early embryos of zebrafish and revealed its biological function for maintaining cell synchrony division because the H2af1o deficiency disturbed cell synchrony in early cleavages before midblastula transition. Therefore, our current findings provided the first case to understand the biological function of maternal oocyte-specific histone variants in vertebrates.
关键词[WOS]: CARASSIUS-AURATUS-GIBELIO ; HISTONE VARIANTS ; LINKER HISTONE ; CLONAL DIVERSITY ; NUCLEOSOME ; CHROMATIN ; EXPRESSION ; BINDING ; EMBRYOGENESIS ; RESOLUTION
语种: 英语
WOS记录号: WOS:000326925800001
ISSN号: 0006-3363
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19774
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Grad Univ, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Fisheries Sci, Key Lab Freshwater Biodivers Conservat, Minist Agr China, Yangtze River Fisheries Res Inst, Wuhan, Peoples R China

Recommended Citation:
Yue, Hua-Mei; Li, Zhi; Wu, Nan; Liu, Zhen; Wang, Yang; Gui, Jian-Fang.Oocyte-Specific H2A Variant H2af1o Is Required for Cell Synchrony Before Midblastula Transition in Early Zebrafish Embryos,BIOLOGY OF REPRODUCTION,2013,89(4):
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