IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Oocyte-Specific H2A Variant H2af1o Is Required for Cell Synchrony Before Midblastula Transition in Early Zebrafish Embryos
Yue, Hua-Mei1,2; Li, Zhi1; Wu, Nan1; Liu, Zhen1; Wang, Yang1; Gui, Jian-Fang1; Gui, JF (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
2013-10-01
Source PublicationBIOLOGY OF REPRODUCTION
ISSN0006-3363
Volume89Issue:4
AbstractOocyte-specific histone variants have been expected to play significant roles in early embryonic development, but the exact evidence and the biological function have remained unclear. Here, we present evidence that H2af1o, an oocyte-specific H2A variant, is required for cell synchrony before midblastula transition in early zebrafish embryos. The H2A variant is oocyte specific, peaks in mature eggs, and is supplied to early embryos. We constructed a series of deletion plasmids of the zebrafish h2af1o tagged with EGFP and determined the main key function regions including nuclear localization signal of N-terminal 25 amino acids and nucleosome binding region of 110-122 amino acid sequence in the C-terminus by microinjecting them into one-cell-stage zebrafish embryos. In comparison with ubiquitous H2A. X, the H2af1o was revealed to confer a more open structure than canonical H2A in the nucleosomes. Furthermore, we conducted the h2af1o-specific morpholino knockdown analysis in early embryos of zebrafish and revealed its biological function for maintaining cell synchrony division because the H2af1o deficiency disturbed cell synchrony in early cleavages before midblastula transition. Therefore, our current findings provided the first case to understand the biological function of maternal oocyte-specific histone variants in vertebrates.; Oocyte-specific histone variants have been expected to play significant roles in early embryonic development, but the exact evidence and the biological function have remained unclear. Here, we present evidence that H2af1o, an oocyte-specific H2A variant, is required for cell synchrony before midblastula transition in early zebrafish embryos. The H2A variant is oocyte specific, peaks in mature eggs, and is supplied to early embryos. We constructed a series of deletion plasmids of the zebrafish h2af1o tagged with EGFP and determined the main key function regions including nuclear localization signal of N-terminal 25 amino acids and nucleosome binding region of 110-122 amino acid sequence in the C-terminus by microinjecting them into one-cell-stage zebrafish embryos. In comparison with ubiquitous H2A. X, the H2af1o was revealed to confer a more open structure than canonical H2A in the nucleosomes. Furthermore, we conducted the h2af1o-specific morpholino knockdown analysis in early embryos of zebrafish and revealed its biological function for maintaining cell synchrony division because the H2af1o deficiency disturbed cell synchrony in early cleavages before midblastula transition. Therefore, our current findings provided the first case to understand the biological function of maternal oocyte-specific histone variants in vertebrates.
SubtypeArticle
KeywordCell Synchrony Early Cleavage Histone Variant Midblastula Transition Oocyte-specific Histone
Department[Yue, Hua-Mei ; Li, Zhi ; Wu, Nan ; Liu, Zhen ; Wang, Yang ; Gui, Jian-Fang] Chinese Acad Sci, Inst Hydrobiol, Grad Univ, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Yue, Hua-Mei] Chinese Acad Fisheries Sci, Key Lab Freshwater Biodivers Conservat, Minist Agr China, Yangtze River Fisheries Res Inst, Wuhan, Peoples R China
DOI10.1095/biolreprod.113.108043
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Key Basic Research Program of China [2010CB126301]; Special Fund for Agro-scientific Research in the Public Interest [200903046]; Chinese Academy of Sciences [KSCX3-EW-N-04]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17] ; National Key Basic Research Program of China [2010CB126301]; Special Fund for Agro-scientific Research in the Public Interest [200903046]; Chinese Academy of Sciences [KSCX3-EW-N-04]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17] ; National Key Basic Research Program of China [2010CB126301]; Special Fund for Agro-scientific Research in the Public Interest [200903046]; Chinese Academy of Sciences [KSCX3-EW-N-04]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17] ; National Key Basic Research Program of China [2010CB126301]; Special Fund for Agro-scientific Research in the Public Interest [200903046]; Chinese Academy of Sciences [KSCX3-EW-N-04]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17]
Indexed BySCI
Language英语
WOS Research AreaReproductive Biology
WOS SubjectReproductive Biology
WOS IDWOS:000326925800001
WOS KeywordCARASSIUS-AURATUS-GIBELIO ; HISTONE VARIANTS ; LINKER HISTONE ; CLONAL DIVERSITY ; NUCLEOSOME ; CHROMATIN ; EXPRESSION ; BINDING ; EMBRYOGENESIS ; RESOLUTION
Funding OrganizationNational Key Basic Research Program of China [2010CB126301]; Special Fund for Agro-scientific Research in the Public Interest [200903046]; Chinese Academy of Sciences [KSCX3-EW-N-04]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17] ; National Key Basic Research Program of China [2010CB126301]; Special Fund for Agro-scientific Research in the Public Interest [200903046]; Chinese Academy of Sciences [KSCX3-EW-N-04]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17] ; National Key Basic Research Program of China [2010CB126301]; Special Fund for Agro-scientific Research in the Public Interest [200903046]; Chinese Academy of Sciences [KSCX3-EW-N-04]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17] ; National Key Basic Research Program of China [2010CB126301]; Special Fund for Agro-scientific Research in the Public Interest [200903046]; Chinese Academy of Sciences [KSCX3-EW-N-04]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ17]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19774
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorGui, JF (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Grad Univ, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Fisheries Sci, Key Lab Freshwater Biodivers Conservat, Minist Agr China, Yangtze River Fisheries Res Inst, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Yue, Hua-Mei,Li, Zhi,Wu, Nan,et al. Oocyte-Specific H2A Variant H2af1o Is Required for Cell Synchrony Before Midblastula Transition in Early Zebrafish Embryos[J]. BIOLOGY OF REPRODUCTION,2013,89(4).
APA Yue, Hua-Mei.,Li, Zhi.,Wu, Nan.,Liu, Zhen.,Wang, Yang.,...&Gui, JF .(2013).Oocyte-Specific H2A Variant H2af1o Is Required for Cell Synchrony Before Midblastula Transition in Early Zebrafish Embryos.BIOLOGY OF REPRODUCTION,89(4).
MLA Yue, Hua-Mei,et al."Oocyte-Specific H2A Variant H2af1o Is Required for Cell Synchrony Before Midblastula Transition in Early Zebrafish Embryos".BIOLOGY OF REPRODUCTION 89.4(2013).
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