| Apolipoprotein C1 regulates epiboly during gastrulation in zebrafish | |
| Wang Yang; Zhou Li; Li Zhi; Li WenHua; Gui JianFang; Gui, JF (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China. | |
| 2013-11-01 | |
| Source Publication | SCIENCE CHINA-LIFE SCIENCES
 |
| ISSN | 1674-7305 |
| Volume | 56Issue:11Pages:975-984 |
| Abstract | Apolipoprotein C1 (Apoc1) is associated with lipoprotein metabolism, but its physiological role during embryogenesis is largely unknown. We reveal a new function of Apoc1b, a transcript isoform of Apoc1, in epiboly during zebrafish gastrulation. Apoc1b is expressed in yolk syncytial layers and in deep cells of the ventral and lateral region of the embryos. It displays a radial gradient with high levels in the interior layer and low levels in the superficial layer. Knockdown of Apoc1b by injecting antisense morpholino (MO) caused the epiboly arrest in deep cells. Moreover, we show that the radial intercalation and the radial gradient distribution of E-cadherin are disrupted both in Apoc1b knockdown and overexpressed embryos. Therefore, Apoc1b controls epiboly via E-cadherin-mediated radial intercalation in a gradient-dependent manner.; Apolipoprotein C1 (Apoc1) is associated with lipoprotein metabolism, but its physiological role during embryogenesis is largely unknown. We reveal a new function of Apoc1b, a transcript isoform of Apoc1, in epiboly during zebrafish gastrulation. Apoc1b is expressed in yolk syncytial layers and in deep cells of the ventral and lateral region of the embryos. It displays a radial gradient with high levels in the interior layer and low levels in the superficial layer. Knockdown of Apoc1b by injecting antisense morpholino (MO) caused the epiboly arrest in deep cells. Moreover, we show that the radial intercalation and the radial gradient distribution of E-cadherin are disrupted both in Apoc1b knockdown and overexpressed embryos. Therefore, Apoc1b controls epiboly via E-cadherin-mediated radial intercalation in a gradient-dependent manner. |
| Subtype | Article |
| Keyword | Epiboly Apolipoprotein C1 E-cadherin Radial Intercalation |
| Department | [Wang Yang ; Zhou Li ; Li Zhi ; Li WenHua ; Gui JianFang] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| DOI | 10.1007/s11427-013-4563-4 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | National Basic Research Program of China [2010CB126300]; National High Technology Research and Development Program of China [2012AA092201-3]; Ministry of Agriculture of China [200903045]; National Natural Science Foundation of China [30971414] ; National Basic Research Program of China [2010CB126300]; National High Technology Research and Development Program of China [2012AA092201-3]; Ministry of Agriculture of China [200903045]; National Natural Science Foundation of China [30971414] ; National Basic Research Program of China [2010CB126300]; National High Technology Research and Development Program of China [2012AA092201-3]; Ministry of Agriculture of China [200903045]; National Natural Science Foundation of China [30971414] ; National Basic Research Program of China [2010CB126300]; National High Technology Research and Development Program of China [2012AA092201-3]; Ministry of Agriculture of China [200903045]; National Natural Science Foundation of China [30971414] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Life Sciences & Biomedicine - Other Topics |
| WOS Subject | Biology |
| WOS ID | WOS:000326813300001 |
| WOS Keyword | ORANGE-SPOTTED GROUPER ; CELL MOVEMENTS ; VERTEBRATE GASTRULATION ; MOLECULAR-CLONING ; E-CADHERIN ; EXPRESSION ; YOLK ; MICROFILAMENTS ; ORGANIZATION ; CYTOSKELETON |
| Funding Organization | National Basic Research Program of China [2010CB126300]; National High Technology Research and Development Program of China [2012AA092201-3]; Ministry of Agriculture of China [200903045]; National Natural Science Foundation of China [30971414] ; National Basic Research Program of China [2010CB126300]; National High Technology Research and Development Program of China [2012AA092201-3]; Ministry of Agriculture of China [200903045]; National Natural Science Foundation of China [30971414] ; National Basic Research Program of China [2010CB126300]; National High Technology Research and Development Program of China [2012AA092201-3]; Ministry of Agriculture of China [200903045]; National Natural Science Foundation of China [30971414] ; National Basic Research Program of China [2010CB126300]; National High Technology Research and Development Program of China [2012AA092201-3]; Ministry of Agriculture of China [200903045]; National Natural Science Foundation of China [30971414] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/19772 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Corresponding Author | Gui, JF (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China. |
| Affiliation | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Wang Yang,Zhou Li,Li Zhi,et al. Apolipoprotein C1 regulates epiboly during gastrulation in zebrafish[J]. SCIENCE CHINA-LIFE SCIENCES,2013,56(11):975-984. |
| APA | Wang Yang,Zhou Li,Li Zhi,Li WenHua,Gui JianFang,&Gui, JF .(2013).Apolipoprotein C1 regulates epiboly during gastrulation in zebrafish.SCIENCE CHINA-LIFE SCIENCES,56(11),975-984. |
| MLA | Wang Yang,et al."Apolipoprotein C1 regulates epiboly during gastrulation in zebrafish".SCIENCE CHINA-LIFE SCIENCES 56.11(2013):975-984. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Apolipoprotein C1 re（1347KB） | 开放获取 | CC BY-NC-SA | View Application Full Text | |||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment