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A second generation genetic linkage map for silver carp (Hypophthalmichehys molitrix) using microsatellite markers
Guo, Wenjie1,3; Tong, Jingou1; Yu, Xiaomu1; Zhu, Chuankun1,3; Feng, Xiu1,3; Fu, Beide2,3; He, Shunping2; Zeng, Fanzhen1,3; Wang, Xinhua1,3; Liu, Haiyang1,3; Liu, Lusha1,3; Tong, JG (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
2013-11-01
Source PublicationAQUACULTURE
ISSN0044-8486
Volume412Pages:97-106
AbstractIn the study, we constructed a second generation genetic linkage map for silver carp (Hypophthalmichthys molitrix) using anonymous and EST-derived microsatellite markers in a mapping panel containing 156 "pseudo BC" progenies from two interspecific crosses between silver carp and bighead carp (Aristichthys nobilis). A total of 703 markers were ordered on 24 linkage groups (LGs) which are equal to chromosome numbers of the haploid genome of the species. The consensus map spanned 1561.1 cM covering 93.1% of the silver carp genome with an average resolution of 2.2 cM/locus. Length of LGs ranged from 42.1 cM to 97.8 cM(mean 65.0 cM). Total number of markers on individual LG varied from 13 to 56 (mean 29.3). Estimated total length of the female map (1809.0 cM) was 1.52 times longer than that of the male map (1188.5 cM), and the recombination ratio between sexes (female vs. male) was 2.2, showing markedly higher recombination in the females. Percentage of distorted loci in the male map was obviously higher than that in the female map, and 5 segregation distortion regions were identified in the male linkage groups. This second generation genetic linkage map evidently extends previous genetic maps for silver carp, and provides a basis for such studies as quantitative trait locus mapping, comparative genomics and marker-assisted selection. (C) 2013 Elsevier B. V. All rights reserved.; In the study, we constructed a second generation genetic linkage map for silver carp (Hypophthalmichthys molitrix) using anonymous and EST-derived microsatellite markers in a mapping panel containing 156 "pseudo BC" progenies from two interspecific crosses between silver carp and bighead carp (Aristichthys nobilis). A total of 703 markers were ordered on 24 linkage groups (LGs) which are equal to chromosome numbers of the haploid genome of the species. The consensus map spanned 1561.1 cM covering 93.1% of the silver carp genome with an average resolution of 2.2 cM/locus. Length of LGs ranged from 42.1 cM to 97.8 cM(mean 65.0 cM). Total number of markers on individual LG varied from 13 to 56 (mean 29.3). Estimated total length of the female map (1809.0 cM) was 1.52 times longer than that of the male map (1188.5 cM), and the recombination ratio between sexes (female vs. male) was 2.2, showing markedly higher recombination in the females. Percentage of distorted loci in the male map was obviously higher than that in the female map, and 5 segregation distortion regions were identified in the male linkage groups. This second generation genetic linkage map evidently extends previous genetic maps for silver carp, and provides a basis for such studies as quantitative trait locus mapping, comparative genomics and marker-assisted selection. (C) 2013 Elsevier B. V. All rights reserved.
SubtypeArticle
KeywordSilver Carp (Hypophthalmichehys Molitrix) Genetic Linkage Map Microsatellite (Ssr) Pseudo-testcross Recombination Rate Segregation Distortion
Department[Guo, Wenjie ; Tong, Jingou ; Yu, Xiaomu ; Zhu, Chuankun ; Feng, Xiu ; Zeng, Fanzhen ; Wang, Xinhua ; Liu, Haiyang ; Liu, Lusha] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Fu, Beide ; He, Shunping] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat CAS, Wuhan 430072, Peoples R China ; [Guo, Wenjie ; Zhu, Chuankun ; Feng, Xiu ; Fu, Beide ; Zeng, Fanzhen ; Wang, Xinhua ; Liu, Haiyang ; Liu, Lusha] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
DOI10.1016/j.aquaculture.2013.06.027
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNSFC [30771643, 31272647]; MOA [200903045]; MOST 973 [2010CB126305]; FEBL of China [2011FBZ20] ; NSFC [30771643, 31272647]; MOA [200903045]; MOST 973 [2010CB126305]; FEBL of China [2011FBZ20] ; NSFC [30771643, 31272647]; MOA [200903045]; MOST 973 [2010CB126305]; FEBL of China [2011FBZ20] ; NSFC [30771643, 31272647]; MOA [200903045]; MOST 973 [2010CB126305]; FEBL of China [2011FBZ20]
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000326163000014
WOS KeywordCOMPARATIVE GENOME ANALYSIS ; TROUT ONCORHYNCHUS-MYKISS ; CROSS-SPECIES APPLICATION ; SALMO-SALAR REVEALS ; ARISTICHTHYS-NOBILIS ; RECOMBINATION RATES ; CTENOPHARYNGODON-IDELLA ; SEGREGATION DISTORTION ; PARALICHTHYS-OLIVACEUS ; JAPANESE FLOUNDER
Funding OrganizationNSFC [30771643, 31272647]; MOA [200903045]; MOST 973 [2010CB126305]; FEBL of China [2011FBZ20] ; NSFC [30771643, 31272647]; MOA [200903045]; MOST 973 [2010CB126305]; FEBL of China [2011FBZ20] ; NSFC [30771643, 31272647]; MOA [200903045]; MOST 973 [2010CB126305]; FEBL of China [2011FBZ20] ; NSFC [30771643, 31272647]; MOA [200903045]; MOST 973 [2010CB126305]; FEBL of China [2011FBZ20]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19771
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorTong, JG (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat CAS, Wuhan 430072, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Guo, Wenjie,Tong, Jingou,Yu, Xiaomu,et al. A second generation genetic linkage map for silver carp (Hypophthalmichehys molitrix) using microsatellite markers[J]. AQUACULTURE,2013,412:97-106.
APA Guo, Wenjie.,Tong, Jingou.,Yu, Xiaomu.,Zhu, Chuankun.,Feng, Xiu.,...&Tong, JG .(2013).A second generation genetic linkage map for silver carp (Hypophthalmichehys molitrix) using microsatellite markers.AQUACULTURE,412,97-106.
MLA Guo, Wenjie,et al."A second generation genetic linkage map for silver carp (Hypophthalmichehys molitrix) using microsatellite markers".AQUACULTURE 412(2013):97-106.
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