IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Molecular characterization of Streptococcus agalactiae in diseased farmed tilapia in China
Zhang, Defeng1,2,4; Li, Aihua1; Guo, Yujuan3; Zhang, Qianqian1,2; Chen, Xuenian3; Gong, Xiaoning1; Li, AH (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
2013-11-01
Source PublicationAQUACULTURE
ISSN0044-8486
Volume412Pages:64-69
AbstractA severe outbreak of streptococcosis in cultured tilapia (Oreochromis niloticus) caused by Streptococcus agalactiae (Group B streptococcus, GBS) has occurred annually in Southern China during the past years, causing significant economic losses. Little is known about the genetic characteristics of the tilapia S. agalactiae that is prevalent in these areas. A total of 51 GBS isolates from tilapia were collected from 27 farms located in distinct geographic regions. The genetic characteristics of GBS were analyzed by MLST, MLVA, serotyping, and PCR screening of mobile genetic elements, genetic marker, and virulence-related genes. The results showed that all of S. agalactiae tilapia isolates have the genotype, Ia-ST7-bac-bca-fbsA-sip-cfb-IS1381-IS861-GBSil-ISSag2, which suggests a low level of genetic diversity and sharing of a recent and similar origin and a low level of genetic differentiation under similar environment selective pressures. Compared with the control, significant differences were detected between the tilapia strains and the S. agalactiae strains of human and bovine origin. Phage typing successfully differentiated all 51 tilapia GBS isolates into two distinct molecular types (type A and B). Interestingly, the phage type of the tilapia GBS isolates was also observed to shift from type A to type B, with the year 2010 as the turning point. Type B GBS isolates are currently prevalent in tilapia populations in China, and should thus be the target strains to detect and develop prophylactic-therapeutic measures. The genetic data obtained in the present study will be helpful in the determination of the epidemiology of tilapia streptococcosis internationally. (C) 2013 Elsevier B. V. All rights reserved.; A severe outbreak of streptococcosis in cultured tilapia (Oreochromis niloticus) caused by Streptococcus agalactiae (Group B streptococcus, GBS) has occurred annually in Southern China during the past years, causing significant economic losses. Little is known about the genetic characteristics of the tilapia S. agalactiae that is prevalent in these areas. A total of 51 GBS isolates from tilapia were collected from 27 farms located in distinct geographic regions. The genetic characteristics of GBS were analyzed by MLST, MLVA, serotyping, and PCR screening of mobile genetic elements, genetic marker, and virulence-related genes. The results showed that all of S. agalactiae tilapia isolates have the genotype, Ia-ST7-bac-bca-fbsA-sip-cfb-IS1381-IS861-GBSil-ISSag2, which suggests a low level of genetic diversity and sharing of a recent and similar origin and a low level of genetic differentiation under similar environment selective pressures. Compared with the control, significant differences were detected between the tilapia strains and the S. agalactiae strains of human and bovine origin. Phage typing successfully differentiated all 51 tilapia GBS isolates into two distinct molecular types (type A and B). Interestingly, the phage type of the tilapia GBS isolates was also observed to shift from type A to type B, with the year 2010 as the turning point. Type B GBS isolates are currently prevalent in tilapia populations in China, and should thus be the target strains to detect and develop prophylactic-therapeutic measures. The genetic data obtained in the present study will be helpful in the determination of the epidemiology of tilapia streptococcosis internationally. (C) 2013 Elsevier B. V. All rights reserved.
SubtypeArticle
KeywordStreptococcus Agalactiae Tilapia Molecular Genotype Epidemiology
Department[Zhang, Defeng ; Li, Aihua ; Zhang, Qianqian ; Gong, Xiaoning] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Zhang, Defeng ; Zhang, Qianqian] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Guo, Yujuan ; Chen, Xuenian] Zhaoqing Univ, Coll life Sci, Zhaoqing 526061, Peoples R China ; [Zhang, Defeng] Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China
DOI10.1016/j.aquaculture.2013.07.014
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNatural Science Foundation of China [30670112, 31070112]; CAS-Guangdong Joint Project [2011B090300036]; FEBL Project [2011FBZ26] ; Natural Science Foundation of China [30670112, 31070112]; CAS-Guangdong Joint Project [2011B090300036]; FEBL Project [2011FBZ26] ; Natural Science Foundation of China [30670112, 31070112]; CAS-Guangdong Joint Project [2011B090300036]; FEBL Project [2011FBZ26] ; Natural Science Foundation of China [30670112, 31070112]; CAS-Guangdong Joint Project [2011B090300036]; FEBL Project [2011FBZ26]
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000326163000009
WOS KeywordCOMPLETE GENOME SEQUENCE ; MULTIPLEX PCR ASSAY ; RED TILAPIA ; OREOCHROMIS-NILOTICUS ; DIRECT IDENTIFICATION ; PFGE GENOTYPE ; FISH ; STRAINS ; GENES ; MENINGOENCEPHALITIS
Funding OrganizationNatural Science Foundation of China [30670112, 31070112]; CAS-Guangdong Joint Project [2011B090300036]; FEBL Project [2011FBZ26] ; Natural Science Foundation of China [30670112, 31070112]; CAS-Guangdong Joint Project [2011B090300036]; FEBL Project [2011FBZ26] ; Natural Science Foundation of China [30670112, 31070112]; CAS-Guangdong Joint Project [2011B090300036]; FEBL Project [2011FBZ26] ; Natural Science Foundation of China [30670112, 31070112]; CAS-Guangdong Joint Project [2011B090300036]; FEBL Project [2011FBZ26]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19770
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorLi, AH (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Zhaoqing Univ, Coll life Sci, Zhaoqing 526061, Peoples R China
4.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Defeng,Li, Aihua,Guo, Yujuan,et al. Molecular characterization of Streptococcus agalactiae in diseased farmed tilapia in China[J]. AQUACULTURE,2013,412:64-69.
APA Zhang, Defeng.,Li, Aihua.,Guo, Yujuan.,Zhang, Qianqian.,Chen, Xuenian.,...&Li, AH .(2013).Molecular characterization of Streptococcus agalactiae in diseased farmed tilapia in China.AQUACULTURE,412,64-69.
MLA Zhang, Defeng,et al."Molecular characterization of Streptococcus agalactiae in diseased farmed tilapia in China".AQUACULTURE 412(2013):64-69.
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