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题名: Isolation and identification of a lethal rhabdovirus from farmed rice field eels Monopterus albus
作者: Ou, Tong1; Zhu, Ruo-Lin1; Chen, Zhong-Yuan1; Zhang, Qi-Ya1
通讯作者: Zhang, QY (reprint author), Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
关键词: Fish viral disease ; Rice field eel ; Monopterus albus rhabdovirus ; MoARV ; Histopathology ; Electron microscopy ; Phylogenetic analysis
刊名: DISEASES OF AQUATIC ORGANISMS
发表日期: 2013-11-06
DOI: 10.3354/dao02660
卷: 106, 期:3, 页:197-206
收录类别: SCI
文章类型: Article
部门归属: [Ou, Tong ; Zhu, Ruo-Lin ; Chen, Zhong-Yuan ; Zhang, Qi-Ya] Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Major Basic Research Program [2010CB-126303]; National Natural Science Foundation of China [31072239, 31270213]; FEBL research grant [2011-FBZ12, 2008FBZ16]
类目[WOS]: Fisheries ; Veterinary Sciences
研究领域[WOS]: Fisheries ; Veterinary Sciences
摘要: We provide the first description of a virus responsible for a systemic hemorrhagic disease causing high mortality in farmed rice field eels Monopterus albus in China. Typical signs exhibited by the diseased fish were extensive hemorrhages in the skin and viscera and some neuro logical signs, such as loss of equilibrium and disorganized swimming. Histopathological examination revealed various degrees of necrosis within the spleen and liver. Virus isolation was attempted from visceral tissues of diseased fish by inoculation on 6 fish cell lines. Typical cytopathic effects (CPE) were produced in bluegill fry (BF2) cells, so this cell line was chosen for further isolation and propagation of the virus. Electron microscopy observation showed that the negative stained viral particles had the characteristic bullet shape of rhabdoviruses and an estimated size of 60 x 120 nm. We therefore tentatively refer to this virus as Monopterus albus rhabdovirus (MoARV). Molecular characterization of MoARV, including sequence analysis of the nucleoprotein (N), phosphoprotein (P), and glycoprotein (G) genes, revealed 94.5 to 97.3% amino acid similarity to that of Siniperca chuatsi rhabdovirus. Phylogenetic analysis based on the amino acid sequences of N and G proteins indicated that MoARV should be a member of the genus Vesiculovirus. Koch's postulates were fulfilled by infecting healthy rice field eels with MoARV, which produced an acute infection. RT-PCR analysis demonstrated that MoARV RNA could be detected in both naturally and experimentally infected fish. The data suggest that MoARV was the causative pathogen of the disease.
英文摘要: We provide the first description of a virus responsible for a systemic hemorrhagic disease causing high mortality in farmed rice field eels Monopterus albus in China. Typical signs exhibited by the diseased fish were extensive hemorrhages in the skin and viscera and some neuro logical signs, such as loss of equilibrium and disorganized swimming. Histopathological examination revealed various degrees of necrosis within the spleen and liver. Virus isolation was attempted from visceral tissues of diseased fish by inoculation on 6 fish cell lines. Typical cytopathic effects (CPE) were produced in bluegill fry (BF2) cells, so this cell line was chosen for further isolation and propagation of the virus. Electron microscopy observation showed that the negative stained viral particles had the characteristic bullet shape of rhabdoviruses and an estimated size of 60 x 120 nm. We therefore tentatively refer to this virus as Monopterus albus rhabdovirus (MoARV). Molecular characterization of MoARV, including sequence analysis of the nucleoprotein (N), phosphoprotein (P), and glycoprotein (G) genes, revealed 94.5 to 97.3% amino acid similarity to that of Siniperca chuatsi rhabdovirus. Phylogenetic analysis based on the amino acid sequences of N and G proteins indicated that MoARV should be a member of the genus Vesiculovirus. Koch's postulates were fulfilled by infecting healthy rice field eels with MoARV, which produced an acute infection. RT-PCR analysis demonstrated that MoARV RNA could be detected in both naturally and experimentally infected fish. The data suggest that MoARV was the causative pathogen of the disease.
关键词[WOS]: HEMORRHAGIC SEPTICEMIA VIRUS ; MANDARIN FISH ; GENOMIC SEQUENCE ; SPRING VIREMIA ; CARP VIRUS ; EUROPEAN X ; MORTALITY ; POSITION ; PERCH ; GENE
语种: 英语
WOS记录号: WOS:000326653800002
ISSN号: 0177-5103
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19769
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

Recommended Citation:
Ou, Tong; Zhu, Ruo-Lin; Chen, Zhong-Yuan; Zhang, Qi-Ya.Isolation and identification of a lethal rhabdovirus from farmed rice field eels Monopterus albus,DISEASES OF AQUATIC ORGANISMS,2013,106(3):197-206
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