IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Isolation and identification of a lethal rhabdovirus from farmed rice field eels Monopterus albus
Ou, Tong; Zhu, Ruo-Lin; Chen, Zhong-Yuan; Zhang, Qi-Ya; Zhang, QY (reprint author), Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
2013-11-06
Source PublicationDISEASES OF AQUATIC ORGANISMS
ISSN0177-5103
Volume106Issue:3Pages:197-206
AbstractWe provide the first description of a virus responsible for a systemic hemorrhagic disease causing high mortality in farmed rice field eels Monopterus albus in China. Typical signs exhibited by the diseased fish were extensive hemorrhages in the skin and viscera and some neuro logical signs, such as loss of equilibrium and disorganized swimming. Histopathological examination revealed various degrees of necrosis within the spleen and liver. Virus isolation was attempted from visceral tissues of diseased fish by inoculation on 6 fish cell lines. Typical cytopathic effects (CPE) were produced in bluegill fry (BF2) cells, so this cell line was chosen for further isolation and propagation of the virus. Electron microscopy observation showed that the negative stained viral particles had the characteristic bullet shape of rhabdoviruses and an estimated size of 60 x 120 nm. We therefore tentatively refer to this virus as Monopterus albus rhabdovirus (MoARV). Molecular characterization of MoARV, including sequence analysis of the nucleoprotein (N), phosphoprotein (P), and glycoprotein (G) genes, revealed 94.5 to 97.3% amino acid similarity to that of Siniperca chuatsi rhabdovirus. Phylogenetic analysis based on the amino acid sequences of N and G proteins indicated that MoARV should be a member of the genus Vesiculovirus. Koch's postulates were fulfilled by infecting healthy rice field eels with MoARV, which produced an acute infection. RT-PCR analysis demonstrated that MoARV RNA could be detected in both naturally and experimentally infected fish. The data suggest that MoARV was the causative pathogen of the disease.; We provide the first description of a virus responsible for a systemic hemorrhagic disease causing high mortality in farmed rice field eels Monopterus albus in China. Typical signs exhibited by the diseased fish were extensive hemorrhages in the skin and viscera and some neuro logical signs, such as loss of equilibrium and disorganized swimming. Histopathological examination revealed various degrees of necrosis within the spleen and liver. Virus isolation was attempted from visceral tissues of diseased fish by inoculation on 6 fish cell lines. Typical cytopathic effects (CPE) were produced in bluegill fry (BF2) cells, so this cell line was chosen for further isolation and propagation of the virus. Electron microscopy observation showed that the negative stained viral particles had the characteristic bullet shape of rhabdoviruses and an estimated size of 60 x 120 nm. We therefore tentatively refer to this virus as Monopterus albus rhabdovirus (MoARV). Molecular characterization of MoARV, including sequence analysis of the nucleoprotein (N), phosphoprotein (P), and glycoprotein (G) genes, revealed 94.5 to 97.3% amino acid similarity to that of Siniperca chuatsi rhabdovirus. Phylogenetic analysis based on the amino acid sequences of N and G proteins indicated that MoARV should be a member of the genus Vesiculovirus. Koch's postulates were fulfilled by infecting healthy rice field eels with MoARV, which produced an acute infection. RT-PCR analysis demonstrated that MoARV RNA could be detected in both naturally and experimentally infected fish. The data suggest that MoARV was the causative pathogen of the disease.
SubtypeArticle
KeywordFish Viral Disease Rice Field Eel Monopterus Albus Rhabdovirus Moarv Histopathology Electron Microscopy Phylogenetic Analysis
Department[Ou, Tong ; Zhu, Ruo-Lin ; Chen, Zhong-Yuan ; Zhang, Qi-Ya] Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
DOI10.3354/dao02660
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Major Basic Research Program [2010CB-126303]; National Natural Science Foundation of China [31072239, 31270213]; FEBL research grant [2011-FBZ12, 2008FBZ16] ; National Major Basic Research Program [2010CB-126303]; National Natural Science Foundation of China [31072239, 31270213]; FEBL research grant [2011-FBZ12, 2008FBZ16] ; National Major Basic Research Program [2010CB-126303]; National Natural Science Foundation of China [31072239, 31270213]; FEBL research grant [2011-FBZ12, 2008FBZ16] ; National Major Basic Research Program [2010CB-126303]; National Natural Science Foundation of China [31072239, 31270213]; FEBL research grant [2011-FBZ12, 2008FBZ16]
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Veterinary Sciences
WOS SubjectFisheries ; Veterinary Sciences
WOS IDWOS:000326653800002
WOS KeywordHEMORRHAGIC SEPTICEMIA VIRUS ; MANDARIN FISH ; GENOMIC SEQUENCE ; SPRING VIREMIA ; CARP VIRUS ; EUROPEAN X ; MORTALITY ; POSITION ; PERCH ; GENE
Funding OrganizationNational Major Basic Research Program [2010CB-126303]; National Natural Science Foundation of China [31072239, 31270213]; FEBL research grant [2011-FBZ12, 2008FBZ16] ; National Major Basic Research Program [2010CB-126303]; National Natural Science Foundation of China [31072239, 31270213]; FEBL research grant [2011-FBZ12, 2008FBZ16] ; National Major Basic Research Program [2010CB-126303]; National Natural Science Foundation of China [31072239, 31270213]; FEBL research grant [2011-FBZ12, 2008FBZ16] ; National Major Basic Research Program [2010CB-126303]; National Natural Science Foundation of China [31072239, 31270213]; FEBL research grant [2011-FBZ12, 2008FBZ16]
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19769
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorZhang, QY (reprint author), Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
AffiliationChinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Ou, Tong,Zhu, Ruo-Lin,Chen, Zhong-Yuan,et al. Isolation and identification of a lethal rhabdovirus from farmed rice field eels Monopterus albus[J]. DISEASES OF AQUATIC ORGANISMS,2013,106(3):197-206.
APA Ou, Tong,Zhu, Ruo-Lin,Chen, Zhong-Yuan,Zhang, Qi-Ya,&Zhang, QY .(2013).Isolation and identification of a lethal rhabdovirus from farmed rice field eels Monopterus albus.DISEASES OF AQUATIC ORGANISMS,106(3),197-206.
MLA Ou, Tong,et al."Isolation and identification of a lethal rhabdovirus from farmed rice field eels Monopterus albus".DISEASES OF AQUATIC ORGANISMS 106.3(2013):197-206.
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