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IFN-gamma and its receptors in a reptile reveal the evolutionary conservation of type II IFNs in vertebrates
Chen, Shan Nan1,2; Huang, Bei3; Zhang, Xiao Wen1; Li, Ye1; Zhao, Li Juan1; Li, Nan1; Gao, Qian1; Nie, P.1,3; Nie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China.
2013-12-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume41Issue:4Pages:587-596
AbstractIn this study, interferon gamma (IFN-gamma) and interferon gamma receptor (IFN-gamma R) genes have been identified in non-avian reptile, the North American green anole lizard (Anolis carolinensis). Like their counterparts from other jawed vertebrates, lizard IFN-gamma, IFN-gamma R1 and IFN-gamma R2 show conserved features in genomic organizations, gene loci and protein sequences. The IFN-gamma gene has the full cDNA sequence of 936 bp, with 522 bp open reading frame (ORF) encoding 174 amino acids, and has the genomic organization of four exons and three introns as observed in IFN-gamma genes of other classes of vertebrates. The receptors, IFN-gamma R1 and IFN-gamma R2 have the ORF of 1278 and 984 bp, coding for 425 and 327 aa, respectively, with the genome organization of seven exons and six introns. In the gene loci of IFN-gamma, DYRK2, IL22, IL26 and MDM1 are found with conserved synteny in vertebrates, and similar genes adjacent to IFN-gamma R1 and IFN-gamma R2 were also found. These receptors also contain conserved motifs, such as the membrane-proximal region and the C-terminal five residue motif in IFN-gamma R1, and intracellular conservative sequence in IFN-gamma R2, which have been confirmed to mediate down-stream JAK-STAT signaling pathway in mammals. IFN-gamma and its receptors, IFN-gamma R1 and IFN-gamma R2 were constitutively expressed in organs/tissues examined in the lizard, and up-regulated expression of IFN-gamma was observed in organs/tissues examined following the poly(I:C) stimulation, suggesting its antiviral role in lizards. The conserved features of IFN-gamma and its receptors, IFN-gamma R1 and IFN-gamma R2, in gene organization and gene locus as well as in functional domain or motif may imply that the function of type II IFN system is evolutionarily conserved in the green anole lizard, as observed in other classes of vertebrates. (C) 2013 Elsevier Ltd. All rights reserved.; In this study, interferon gamma (IFN-gamma) and interferon gamma receptor (IFN-gamma R) genes have been identified in non-avian reptile, the North American green anole lizard (Anolis carolinensis). Like their counterparts from other jawed vertebrates, lizard IFN-gamma, IFN-gamma R1 and IFN-gamma R2 show conserved features in genomic organizations, gene loci and protein sequences. The IFN-gamma gene has the full cDNA sequence of 936 bp, with 522 bp open reading frame (ORF) encoding 174 amino acids, and has the genomic organization of four exons and three introns as observed in IFN-gamma genes of other classes of vertebrates. The receptors, IFN-gamma R1 and IFN-gamma R2 have the ORF of 1278 and 984 bp, coding for 425 and 327 aa, respectively, with the genome organization of seven exons and six introns. In the gene loci of IFN-gamma, DYRK2, IL22, IL26 and MDM1 are found with conserved synteny in vertebrates, and similar genes adjacent to IFN-gamma R1 and IFN-gamma R2 were also found. These receptors also contain conserved motifs, such as the membrane-proximal region and the C-terminal five residue motif in IFN-gamma R1, and intracellular conservative sequence in IFN-gamma R2, which have been confirmed to mediate down-stream JAK-STAT signaling pathway in mammals. IFN-gamma and its receptors, IFN-gamma R1 and IFN-gamma R2 were constitutively expressed in organs/tissues examined in the lizard, and up-regulated expression of IFN-gamma was observed in organs/tissues examined following the poly(I:C) stimulation, suggesting its antiviral role in lizards. The conserved features of IFN-gamma and its receptors, IFN-gamma R1 and IFN-gamma R2, in gene organization and gene locus as well as in functional domain or motif may imply that the function of type II IFN system is evolutionarily conserved in the green anole lizard, as observed in other classes of vertebrates. (C) 2013 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordIfn-gamma Ifng Ifngr1 Ifngr2 Lizard
Department[Chen, Shan Nan ; Zhang, Xiao Wen ; Li, Ye ; Zhao, Li Juan ; Li, Nan ; Gao, Qian ; Nie, P.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China ; [Chen, Shan Nan] Chinese Acad Sci, Grad Univ, Beijing 10049, Peoples R China ; [Huang, Bei ; Nie, P.] Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China
DOI10.1016/j.dci.2013.07.002
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [31230075]; National Basic Research Programme (973 Programme) of China [2009CB118703]; Jimei University in Xiamen of Fujian Province ; National Natural Science Foundation of China [31230075]; National Basic Research Programme (973 Programme) of China [2009CB118703]; Jimei University in Xiamen of Fujian Province ; National Natural Science Foundation of China [31230075]; National Basic Research Programme (973 Programme) of China [2009CB118703]; Jimei University in Xiamen of Fujian Province ; National Natural Science Foundation of China [31230075]; National Basic Research Programme (973 Programme) of China [2009CB118703]; Jimei University in Xiamen of Fujian Province
Indexed BySCI
Language英语
WOS Research AreaImmunology ; Zoology
WOS SubjectImmunology ; Zoology
WOS IDWOS:000326258500015
WOS KeywordMURINE INTERFERON-GAMMA ; TROUT ONCORHYNCHUS-MYKISS ; EXPRESSION ANALYSIS ; MOLECULAR CHARACTERIZATION ; ALPHA-CHAIN ; IDENTIFICATION ; CLONING ; GENE ; SEQUENCE ; CDNA
Funding OrganizationNational Natural Science Foundation of China [31230075]; National Basic Research Programme (973 Programme) of China [2009CB118703]; Jimei University in Xiamen of Fujian Province ; National Natural Science Foundation of China [31230075]; National Basic Research Programme (973 Programme) of China [2009CB118703]; Jimei University in Xiamen of Fujian Province ; National Natural Science Foundation of China [31230075]; National Basic Research Programme (973 Programme) of China [2009CB118703]; Jimei University in Xiamen of Fujian Province ; National Natural Science Foundation of China [31230075]; National Basic Research Programme (973 Programme) of China [2009CB118703]; Jimei University in Xiamen of Fujian Province
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19768
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorNie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 10049, Peoples R China
3.Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China
Recommended Citation
GB/T 7714
Chen, Shan Nan,Huang, Bei,Zhang, Xiao Wen,et al. IFN-gamma and its receptors in a reptile reveal the evolutionary conservation of type II IFNs in vertebrates[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2013,41(4):587-596.
APA Chen, Shan Nan.,Huang, Bei.,Zhang, Xiao Wen.,Li, Ye.,Zhao, Li Juan.,...&Nie, P .(2013).IFN-gamma and its receptors in a reptile reveal the evolutionary conservation of type II IFNs in vertebrates.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,41(4),587-596.
MLA Chen, Shan Nan,et al."IFN-gamma and its receptors in a reptile reveal the evolutionary conservation of type II IFNs in vertebrates".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 41.4(2013):587-596.
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