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Development of eighteen polymorphic microsatellite markers in Schizopygopsis younghusbandi Regan, 1905 (Ostariophysi: Cyprinidae) in Tibet, China
He, Dekui1; Tang, Weixing1; Li, Chunhua1,2; Chen, Yifeng1; Chen, YF (reprint author), Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan 430072, Peoples R China.
2013-12-01
Source PublicationCONSERVATION GENETICS RESOURCES
ISSN1877-7252
Volume5Issue:4Pages:895-897
AbstractEighteen polymorphic microsatellite primers were developed for Schizopygopsis younghusbandi using an enrichment genomic library and evaluated for PCR using the genomic DNA of 29 individuals from the Lhasa River in Tibet. Eighteen of 70 loci amplified reliable bands revealed a useful degree of polymorphism with a typical multi-banded pattern of polyploid species. The total number of bands per locus ranged from 3 to 30. Average A (i) and H (o) were 3.563 and 0.916, respectively. The heterozygosity expected varied from 0.595 to 0.946 (RCeS) and from 0.580 to 0.923 (RCdS), respectively. These microsatellite primers would be useful in population genetics studies for this species and related species of genus Schizopygopsis.; Eighteen polymorphic microsatellite primers were developed for Schizopygopsis younghusbandi using an enrichment genomic library and evaluated for PCR using the genomic DNA of 29 individuals from the Lhasa River in Tibet. Eighteen of 70 loci amplified reliable bands revealed a useful degree of polymorphism with a typical multi-banded pattern of polyploid species. The total number of bands per locus ranged from 3 to 30. Average A (i) and H (o) were 3.563 and 0.916, respectively. The heterozygosity expected varied from 0.595 to 0.946 (RCeS) and from 0.580 to 0.923 (RCdS), respectively. These microsatellite primers would be useful in population genetics studies for this species and related species of genus Schizopygopsis.
SubtypeArticle
KeywordSchizopygopsis Schizothoracinae Enriched Genomic Library Population Genetics Tibet
Department[He, Dekui; Tang, Weixing; Li, Chunhua; Chen, Yifeng] Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Li, Chunhua] Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
DOI10.1007/s12686-013-9927-3
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNSFC [41030208, 31071900]; Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy Of Sciences ; NSFC [41030208, 31071900]; Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy Of Sciences ; NSFC [41030208, 31071900]; Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy Of Sciences ; NSFC [41030208, 31071900]; Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy Of Sciences
Indexed BySCI
Language英语
WOS Research AreaBiodiversity & Conservation ; Genetics & Heredity
WOS SubjectBiodiversity Conservation ; Genetics & Heredity
WOS IDWOS:000326387800002
WOS KeywordSCHIZOTHORACINE FISHES TELEOSTEI ; MOLECULAR PHYLOGENY ; STRATEGIES ; SEQUENCE ; PROGRAM ; PLATEAU ; RIVER
Funding OrganizationNSFC [41030208, 31071900]; Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy Of Sciences ; NSFC [41030208, 31071900]; Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy Of Sciences ; NSFC [41030208, 31071900]; Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy Of Sciences ; NSFC [41030208, 31071900]; Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy Of Sciences
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19756
Collection水生生物多样性与资源保护研究中心_期刊论文
Corresponding AuthorChen, YF (reprint author), Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
He, Dekui,Tang, Weixing,Li, Chunhua,et al. Development of eighteen polymorphic microsatellite markers in Schizopygopsis younghusbandi Regan, 1905 (Ostariophysi: Cyprinidae) in Tibet, China[J]. CONSERVATION GENETICS RESOURCES,2013,5(4):895-897.
APA He, Dekui,Tang, Weixing,Li, Chunhua,Chen, Yifeng,&Chen, YF .(2013).Development of eighteen polymorphic microsatellite markers in Schizopygopsis younghusbandi Regan, 1905 (Ostariophysi: Cyprinidae) in Tibet, China.CONSERVATION GENETICS RESOURCES,5(4),895-897.
MLA He, Dekui,et al."Development of eighteen polymorphic microsatellite markers in Schizopygopsis younghusbandi Regan, 1905 (Ostariophysi: Cyprinidae) in Tibet, China".CONSERVATION GENETICS RESOURCES 5.4(2013):895-897.
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