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题名: Development of eighteen polymorphic microsatellite markers in Schizopygopsis younghusbandi Regan, 1905 (Ostariophysi: Cyprinidae) in Tibet, China
作者: He, Dekui1; Tang, Weixing1; Li, Chunhua1, 2; Chen, Yifeng1
通讯作者: Chen, YF (reprint author), Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan 430072, Peoples R China.
关键词: Schizopygopsis ; Schizothoracinae ; Enriched genomic library ; Population genetics ; Tibet
刊名: CONSERVATION GENETICS RESOURCES
发表日期: 2013-12-01
DOI: 10.1007/s12686-013-9927-3
卷: 5, 期:4, 页:895-897
收录类别: SCI
文章类型: Article
部门归属: [He, Dekui; Tang, Weixing; Li, Chunhua; Chen, Yifeng] Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Li, Chunhua] Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: NSFC [41030208, 31071900]; Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy Of Sciences
类目[WOS]: Biodiversity Conservation ; Genetics & Heredity
研究领域[WOS]: Biodiversity & Conservation ; Genetics & Heredity
摘要: Eighteen polymorphic microsatellite primers were developed for Schizopygopsis younghusbandi using an enrichment genomic library and evaluated for PCR using the genomic DNA of 29 individuals from the Lhasa River in Tibet. Eighteen of 70 loci amplified reliable bands revealed a useful degree of polymorphism with a typical multi-banded pattern of polyploid species. The total number of bands per locus ranged from 3 to 30. Average A (i) and H (o) were 3.563 and 0.916, respectively. The heterozygosity expected varied from 0.595 to 0.946 (RCeS) and from 0.580 to 0.923 (RCdS), respectively. These microsatellite primers would be useful in population genetics studies for this species and related species of genus Schizopygopsis.
英文摘要: Eighteen polymorphic microsatellite primers were developed for Schizopygopsis younghusbandi using an enrichment genomic library and evaluated for PCR using the genomic DNA of 29 individuals from the Lhasa River in Tibet. Eighteen of 70 loci amplified reliable bands revealed a useful degree of polymorphism with a typical multi-banded pattern of polyploid species. The total number of bands per locus ranged from 3 to 30. Average A (i) and H (o) were 3.563 and 0.916, respectively. The heterozygosity expected varied from 0.595 to 0.946 (RCeS) and from 0.580 to 0.923 (RCdS), respectively. These microsatellite primers would be useful in population genetics studies for this species and related species of genus Schizopygopsis.
关键词[WOS]: SCHIZOTHORACINE FISHES TELEOSTEI ; MOLECULAR PHYLOGENY ; STRATEGIES ; SEQUENCE ; PROGRAM ; PLATEAU ; RIVER
语种: 英语
WOS记录号: WOS:000326387800002
ISSN号: 1877-7252
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19756
Appears in Collections:水生生物多样性与资源保护研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
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文件名: Development of eighteen polymorphic microsatellite markers in Schizopygopsis younghusbandi Regan, 1905 (Ostariophysi Cyprinidae) in Tibet, China.pdf
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