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Efficacy of a dual fluorescence method in detecting the viability of overwintering cyanobacteria
Zhu, T.; Xu, X.; Xu, X (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China.
2013-09-01
Source PublicationLETTERS IN APPLIED MICROBIOLOGY
ISSN0266-8254
Volume57Issue:3Pages:174-180
AbstractChill in the light is the major environmental stress that cyanobacteria encounter in winter. Cyanobacterial cells may acquire chill-light tolerance upon exposure to low temperature in autumn and early winter. We sought to establish the efficacy of the dual fluorescence method in detecting the viability of overwintering cyanobacteria and to provide further evidence for the chill-light tolerance of preconditioned cyanobacteria. Synechocystis sp. PCC 6803 and Microcystis aeruginosa PCC 7806 were exposed to chill (5 degrees C)-light stress with or without pretreatment at 15 degrees C and stained with SYTO 9 and propidium iodide. Live and dead cells were observed under a fluorescence microscope, and the percentage of viable cells was quantified on a microplate reader. The dual fluorescence method showed consistent results with tests of the ability to reinitiate growth. Cell viability was quantitatively correlated with ratio of SYTO 9/propidium iodide fluorescence. Previously, Microcystis colonies in Lake Taihu had been found to accumulate RNA-binding protein 1 in autumn and winter. Use of this method directly showed the viability of such Microcystis colonies throughout the winter.; Chill in the light is the major environmental stress that cyanobacteria encounter in winter. Cyanobacterial cells may acquire chill-light tolerance upon exposure to low temperature in autumn and early winter. We sought to establish the efficacy of the dual fluorescence method in detecting the viability of overwintering cyanobacteria and to provide further evidence for the chill-light tolerance of preconditioned cyanobacteria. Synechocystis sp. PCC 6803 and Microcystis aeruginosa PCC 7806 were exposed to chill (5 degrees C)-light stress with or without pretreatment at 15 degrees C and stained with SYTO 9 and propidium iodide. Live and dead cells were observed under a fluorescence microscope, and the percentage of viable cells was quantified on a microplate reader. The dual fluorescence method showed consistent results with tests of the ability to reinitiate growth. Cell viability was quantitatively correlated with ratio of SYTO 9/propidium iodide fluorescence. Previously, Microcystis colonies in Lake Taihu had been found to accumulate RNA-binding protein 1 in autumn and winter. Use of this method directly showed the viability of such Microcystis colonies throughout the winter.
SubtypeArticle
KeywordAcquired Chill-light Tolerance Dual Fluorescence Assay Overwintering Cyanobacteria Rna-binding Protein 1 Viability
Department[Zhu, T. ; Xu, X.] Chinese Acad Sci, Inst Hydrobiol, CAS Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
DOI10.1111/lam.12095
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [30825003, 31123001]; State Key Basic Research Development Program of China [2008CB418001] ; National Natural Science Foundation of China [30825003, 31123001]; State Key Basic Research Development Program of China [2008CB418001] ; National Natural Science Foundation of China [30825003, 31123001]; State Key Basic Research Development Program of China [2008CB418001] ; National Natural Science Foundation of China [30825003, 31123001]; State Key Basic Research Development Program of China [2008CB418001]
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Microbiology
WOS SubjectBiotechnology & Applied Microbiology ; Microbiology
WOS IDWOS:000322954700002
WOS KeywordCHILL-LIGHT TOLERANCE ; FLOW-CYTOMETRIC ASSESSMENT ; BACTERIAL VIABILITY ; DRINKING-WATER ; MICROCYSTIS ; MICROORGANISMS ; RHODAMINE-123 ; PCC-6803 ; CULTURES ; LAKE
Funding OrganizationNational Natural Science Foundation of China [30825003, 31123001]; State Key Basic Research Development Program of China [2008CB418001] ; National Natural Science Foundation of China [30825003, 31123001]; State Key Basic Research Development Program of China [2008CB418001] ; National Natural Science Foundation of China [30825003, 31123001]; State Key Basic Research Development Program of China [2008CB418001] ; National Natural Science Foundation of China [30825003, 31123001]; State Key Basic Research Development Program of China [2008CB418001]
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19639
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorXu, X (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China.
AffiliationChinese Acad Sci, Inst Hydrobiol, CAS Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Zhu, T.,Xu, X.,Xu, X . Efficacy of a dual fluorescence method in detecting the viability of overwintering cyanobacteria[J]. LETTERS IN APPLIED MICROBIOLOGY,2013,57(3):174-180.
APA Zhu, T.,Xu, X.,&Xu, X .(2013).Efficacy of a dual fluorescence method in detecting the viability of overwintering cyanobacteria.LETTERS IN APPLIED MICROBIOLOGY,57(3),174-180.
MLA Zhu, T.,et al."Efficacy of a dual fluorescence method in detecting the viability of overwintering cyanobacteria".LETTERS IN APPLIED MICROBIOLOGY 57.3(2013):174-180.
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