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题名: Efficacy of a dual fluorescence method in detecting the viability of overwintering cyanobacteria
作者: Zhu, T.1; Xu, X.1
通讯作者: Xu, X (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China.
关键词: acquired chill-light tolerance ; dual fluorescence assay ; overwintering cyanobacteria ; RNA-binding protein 1 ; viability
刊名: LETTERS IN APPLIED MICROBIOLOGY
发表日期: 2013-09-01
DOI: 10.1111/lam.12095
卷: 57, 期:3, 页:174-180
收录类别: SCI
文章类型: Article
部门归属: [Zhu, T. ; Xu, X.] Chinese Acad Sci, Inst Hydrobiol, CAS Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [30825003, 31123001]; State Key Basic Research Development Program of China [2008CB418001]
类目[WOS]: Biotechnology & Applied Microbiology ; Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology ; Microbiology
摘要: Chill in the light is the major environmental stress that cyanobacteria encounter in winter. Cyanobacterial cells may acquire chill-light tolerance upon exposure to low temperature in autumn and early winter. We sought to establish the efficacy of the dual fluorescence method in detecting the viability of overwintering cyanobacteria and to provide further evidence for the chill-light tolerance of preconditioned cyanobacteria. Synechocystis sp. PCC 6803 and Microcystis aeruginosa PCC 7806 were exposed to chill (5 degrees C)-light stress with or without pretreatment at 15 degrees C and stained with SYTO 9 and propidium iodide. Live and dead cells were observed under a fluorescence microscope, and the percentage of viable cells was quantified on a microplate reader. The dual fluorescence method showed consistent results with tests of the ability to reinitiate growth. Cell viability was quantitatively correlated with ratio of SYTO 9/propidium iodide fluorescence. Previously, Microcystis colonies in Lake Taihu had been found to accumulate RNA-binding protein 1 in autumn and winter. Use of this method directly showed the viability of such Microcystis colonies throughout the winter.
英文摘要: Chill in the light is the major environmental stress that cyanobacteria encounter in winter. Cyanobacterial cells may acquire chill-light tolerance upon exposure to low temperature in autumn and early winter. We sought to establish the efficacy of the dual fluorescence method in detecting the viability of overwintering cyanobacteria and to provide further evidence for the chill-light tolerance of preconditioned cyanobacteria. Synechocystis sp. PCC 6803 and Microcystis aeruginosa PCC 7806 were exposed to chill (5 degrees C)-light stress with or without pretreatment at 15 degrees C and stained with SYTO 9 and propidium iodide. Live and dead cells were observed under a fluorescence microscope, and the percentage of viable cells was quantified on a microplate reader. The dual fluorescence method showed consistent results with tests of the ability to reinitiate growth. Cell viability was quantitatively correlated with ratio of SYTO 9/propidium iodide fluorescence. Previously, Microcystis colonies in Lake Taihu had been found to accumulate RNA-binding protein 1 in autumn and winter. Use of this method directly showed the viability of such Microcystis colonies throughout the winter.
关键词[WOS]: CHILL-LIGHT TOLERANCE ; FLOW-CYTOMETRIC ASSESSMENT ; BACTERIAL VIABILITY ; DRINKING-WATER ; MICROCYSTIS ; MICROORGANISMS ; RHODAMINE-123 ; PCC-6803 ; CULTURES ; LAKE
语种: 英语
WOS记录号: WOS:000322954700002
ISSN号: 0266-8254
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19639
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, CAS Key Lab Algal Biol, Wuhan 430072, Hubei, Peoples R China

Recommended Citation:
Zhu, T.; Xu, X..Efficacy of a dual fluorescence method in detecting the viability of overwintering cyanobacteria,LETTERS IN APPLIED MICROBIOLOGY,2013,57(3):174-180
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