中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 藻类生物学及应用研究中心  > 期刊论文
题名: RNase III-dependent down-regulation of ftsH by an artificial internal sense RNA in Anabaena sp PCC 7120
作者: Gao, Yuan1; Gong, Yangmin1; Xu, Xudong1
通讯作者: Xu, XD (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China.
关键词: RNase III activity ; aaftsH interference ; Anabaena
刊名: FEMS MICROBIOLOGY LETTERS
发表日期: 2013-07-01
DOI: 10.1111/1574-6968.12165
卷: 344, 期:2, 页:130-137
收录类别: SCI
文章类型: Article
部门归属: [Gao, Yuan ; Gong, Yangmin ; Xu, Xudong] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
摘要: RNase III is a group of dsRNA-specific ribonucleases that play important roles in RNA processing and metabolism. Alr0280 and All4107 in Anabaena sp. PCC7120 are highly similar to RNase III enzymes. In vitro, recombinant Alr0280 showed RNase III activity. In the same cyanobacterium, the expression of ftsH (FtsH protease) could be suppressed by overexpression of an artificial sense RNA (aaftsH) that was complementary to aftsH, an internal antisense RNA. The aaftsH interference was abolished by inactivation of alr0280, the RNase III-encoding gene, and restored by complementation of the mutant. A cyanobacterial homolog to hen1, an RNA methyltransferase gene, may also be required for the aaftsH interference. This is the first report of RNase III-dependent sense RNA interference in cyanobacteria, and the underlying mechanism remains to be elucidated.
英文摘要: RNase III is a group of dsRNA-specific ribonucleases that play important roles in RNA processing and metabolism. Alr0280 and All4107 in Anabaena sp. PCC7120 are highly similar to RNase III enzymes. In vitro, recombinant Alr0280 showed RNase III activity. In the same cyanobacterium, the expression of ftsH (FtsH protease) could be suppressed by overexpression of an artificial sense RNA (aaftsH) that was complementary to aftsH, an internal antisense RNA. The aaftsH interference was abolished by inactivation of alr0280, the RNase III-encoding gene, and restored by complementation of the mutant. A cyanobacterial homolog to hen1, an RNA methyltransferase gene, may also be required for the aaftsH interference. This is the first report of RNase III-dependent sense RNA interference in cyanobacteria, and the underlying mechanism remains to be elucidated.
关键词[WOS]: DOUBLE-STRANDED-RNA ; SP-STRAIN PCC-7120 ; COLI RIBONUCLEASE-III ; ANTISENSE-RNA ; GENE-EXPRESSION ; IN-VITRO ; HETEROCYST DIFFERENTIATION ; KINASE GENE ; MECHANISM ; DOMAIN
语种: 英语
WOS记录号: WOS:000320722600005
ISSN号: 0378-1097
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19636
Appears in Collections:藻类生物学及应用研究中心_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
RNase III-dependent down-regulation of ftsH by an artificial internal sense RNA in Anabaena sp PCC 7120.pdf(311KB)----开放获取View 联系获取全文

作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China

Recommended Citation:
Gao, Yuan; Gong, Yangmin; Xu, Xudong.RNase III-dependent down-regulation of ftsH by an artificial internal sense RNA in Anabaena sp PCC 7120,FEMS MICROBIOLOGY LETTERS,2013,344(2):130-137
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Gao, Yuan]'s Articles
[Gong, Yangmin]'s Articles
[Xu, Xudong]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Gao, Yuan]‘s Articles
[Gong, Yangmin]‘s Articles
[Xu, Xudong]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: RNase III-dependent down-regulation of ftsH by an artificial internal sense RNA in Anabaena sp PCC 7120.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2016  中国科学院水生生物研究所 - Feedback
Powered by CSpace