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RNase III-dependent down-regulation of ftsH by an artificial internal sense RNA in Anabaena sp PCC 7120
Gao, Yuan; Gong, Yangmin; Xu, Xudong; Xu, XD (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China.
2013-07-01
Source PublicationFEMS MICROBIOLOGY LETTERS
ISSN0378-1097
Volume344Issue:2Pages:130-137
AbstractRNase III is a group of dsRNA-specific ribonucleases that play important roles in RNA processing and metabolism. Alr0280 and All4107 in Anabaena sp. PCC7120 are highly similar to RNase III enzymes. In vitro, recombinant Alr0280 showed RNase III activity. In the same cyanobacterium, the expression of ftsH (FtsH protease) could be suppressed by overexpression of an artificial sense RNA (aaftsH) that was complementary to aftsH, an internal antisense RNA. The aaftsH interference was abolished by inactivation of alr0280, the RNase III-encoding gene, and restored by complementation of the mutant. A cyanobacterial homolog to hen1, an RNA methyltransferase gene, may also be required for the aaftsH interference. This is the first report of RNase III-dependent sense RNA interference in cyanobacteria, and the underlying mechanism remains to be elucidated.; RNase III is a group of dsRNA-specific ribonucleases that play important roles in RNA processing and metabolism. Alr0280 and All4107 in Anabaena sp. PCC7120 are highly similar to RNase III enzymes. In vitro, recombinant Alr0280 showed RNase III activity. In the same cyanobacterium, the expression of ftsH (FtsH protease) could be suppressed by overexpression of an artificial sense RNA (aaftsH) that was complementary to aftsH, an internal antisense RNA. The aaftsH interference was abolished by inactivation of alr0280, the RNase III-encoding gene, and restored by complementation of the mutant. A cyanobacterial homolog to hen1, an RNA methyltransferase gene, may also be required for the aaftsH interference. This is the first report of RNase III-dependent sense RNA interference in cyanobacteria, and the underlying mechanism remains to be elucidated.
SubtypeArticle
KeywordRnase Iii Activity Aaftsh Interference Anabaena
Department[Gao, Yuan ; Gong, Yangmin ; Xu, Xudong] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
DOI10.1111/1574-6968.12165
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000320722600005
WOS KeywordDOUBLE-STRANDED-RNA ; SP-STRAIN PCC-7120 ; COLI RIBONUCLEASE-III ; ANTISENSE-RNA ; GENE-EXPRESSION ; IN-VITRO ; HETEROCYST DIFFERENTIATION ; KINASE GENE ; MECHANISM ; DOMAIN
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19636
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorXu, XD (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China.
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Gao, Yuan,Gong, Yangmin,Xu, Xudong,et al. RNase III-dependent down-regulation of ftsH by an artificial internal sense RNA in Anabaena sp PCC 7120[J]. FEMS MICROBIOLOGY LETTERS,2013,344(2):130-137.
APA Gao, Yuan,Gong, Yangmin,Xu, Xudong,&Xu, XD .(2013).RNase III-dependent down-regulation of ftsH by an artificial internal sense RNA in Anabaena sp PCC 7120.FEMS MICROBIOLOGY LETTERS,344(2),130-137.
MLA Gao, Yuan,et al."RNase III-dependent down-regulation of ftsH by an artificial internal sense RNA in Anabaena sp PCC 7120".FEMS MICROBIOLOGY LETTERS 344.2(2013):130-137.
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