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Nitrogen Starvation Induced Oxidative Stress in an Oil-Producing Green Alga Chlorella sorokiniana C3
Zhang, Yun-Ming1,2; Chen, Hui1; He, Chen-Liu1; Wang, Qiang1; Wang, Q (reprint author), Chinese Acad Sci, Key Lab Algal Biol, Inst Hydrobiol, Wuhan, Hubei Province, Peoples R China.
2013-07-16
Source PublicationPLOS ONE
ISSN1932-6203
Volume8Issue:7Pages:e69225
AbstractMicroalgal lipid is one of the most promising feedstocks for biodiesel production. Chlorella appears to be a particularly good option, and nitrogen (N) starvation is an efficient environmental pressure used to increase lipid accumulation in Chlorella cells. The effects of N starvation of an oil-producing wild microalga, Chlorella sorokiniana C3, on lipid accumulation were investigated using thin layer chromatography (TLC), confocal laser scanning microscopy (CLSM) and flow cytometry (FCM). The results showed that N starvation resulted in lipid accumulation in C. sorokiniana C3 cells, oil droplet (OD) formation and significant lipid accumulation in cells were detected after 2 d and 8 d of N starvation, respectively. During OD formation, reduced photosynthetic rate, respiration rate and photochemistry efficiency accompanied by increased damage to PSII were observed, demonstrated by chlorophyll (Chl) fluorescence, 77K fluorescence and oxygen evolution tests. In the mean time the rate of cyclic electron transportation increased correspondingly to produce more ATP for triacylglycerols (TAGs) synthesis. And 0.5 d was found to be the turning point for the early stress response and acclimation of cells to N starvation. Increased level of membrane peroxidation was also observed during OD formation, and superoxide dismutase (SOD), peroxide dismutase (POD) and catalase (CAT) enzyme activity assays suggested impaired reactive oxygen species (ROS) scavenging ability. Significant neutral lipid accumulation was also observed by artificial oxidative stress induced by H2O2 treatment. These results suggested coupled neutral lipid accumulation and oxidative stress during N starvation in C. sorokiniana C3.; Microalgal lipid is one of the most promising feedstocks for biodiesel production. Chlorella appears to be a particularly good option, and nitrogen (N) starvation is an efficient environmental pressure used to increase lipid accumulation in Chlorella cells. The effects of N starvation of an oil-producing wild microalga, Chlorella sorokiniana C3, on lipid accumulation were investigated using thin layer chromatography (TLC), confocal laser scanning microscopy (CLSM) and flow cytometry (FCM). The results showed that N starvation resulted in lipid accumulation in C. sorokiniana C3 cells, oil droplet (OD) formation and significant lipid accumulation in cells were detected after 2 d and 8 d of N starvation, respectively. During OD formation, reduced photosynthetic rate, respiration rate and photochemistry efficiency accompanied by increased damage to PSII were observed, demonstrated by chlorophyll (Chl) fluorescence, 77K fluorescence and oxygen evolution tests. In the mean time the rate of cyclic electron transportation increased correspondingly to produce more ATP for triacylglycerols (TAGs) synthesis. And 0.5 d was found to be the turning point for the early stress response and acclimation of cells to N starvation. Increased level of membrane peroxidation was also observed during OD formation, and superoxide dismutase (SOD), peroxide dismutase (POD) and catalase (CAT) enzyme activity assays suggested impaired reactive oxygen species (ROS) scavenging ability. Significant neutral lipid accumulation was also observed by artificial oxidative stress induced by H2O2 treatment. These results suggested coupled neutral lipid accumulation and oxidative stress during N starvation in C. sorokiniana C3.
SubtypeArticle
KeywordBiodiesel Production Lipid-accumulation Photosystem-i Microalgae Fluorescence Vulgaris Energy Light Antioxidants Carotenoids
Department[Zhang, Yun-Ming ; Chen, Hui ; He, Chen-Liu ; Wang, Qiang] Chinese Acad Sci, Key Lab Algal Biol, Inst Hydrobiol, Wuhan, Hubei Province, Peoples R China ; [Zhang, Yun-Ming] Univ Chinese Acad Sci, Beijing, Peoples R China
DOI10.1371/journal.pone.0069225
WOS HeadingsScience & Technology
Funding OrganizationNational Program on Key Basic Research Project [2012CB224803]; Chinese Academy of Sciences [Y05101-1-601] ; National Program on Key Basic Research Project [2012CB224803]; Chinese Academy of Sciences [Y05101-1-601] ; National Program on Key Basic Research Project [2012CB224803]; Chinese Academy of Sciences [Y05101-1-601] ; National Program on Key Basic Research Project [2012CB224803]; Chinese Academy of Sciences [Y05101-1-601]
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000322064300093
WOS KeywordBIODIESEL PRODUCTION ; LIPID-ACCUMULATION ; PHOTOSYSTEM-I ; MICROALGAE ; FLUORESCENCE ; VULGARIS ; ENERGY ; LIGHT ; ANTIOXIDANTS ; CAROTENOIDS
Funding OrganizationNational Program on Key Basic Research Project [2012CB224803]; Chinese Academy of Sciences [Y05101-1-601] ; National Program on Key Basic Research Project [2012CB224803]; Chinese Academy of Sciences [Y05101-1-601] ; National Program on Key Basic Research Project [2012CB224803]; Chinese Academy of Sciences [Y05101-1-601] ; National Program on Key Basic Research Project [2012CB224803]; Chinese Academy of Sciences [Y05101-1-601]
Citation statistics
Cited Times:92[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19632
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorWang, Q (reprint author), Chinese Acad Sci, Key Lab Algal Biol, Inst Hydrobiol, Wuhan, Hubei Province, Peoples R China.
Affiliation1.Chinese Acad Sci, Key Lab Algal Biol, Inst Hydrobiol, Wuhan, Hubei Province, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Yun-Ming,Chen, Hui,He, Chen-Liu,et al. Nitrogen Starvation Induced Oxidative Stress in an Oil-Producing Green Alga Chlorella sorokiniana C3[J]. PLOS ONE,2013,8(7):e69225.
APA Zhang, Yun-Ming,Chen, Hui,He, Chen-Liu,Wang, Qiang,&Wang, Q .(2013).Nitrogen Starvation Induced Oxidative Stress in an Oil-Producing Green Alga Chlorella sorokiniana C3.PLOS ONE,8(7),e69225.
MLA Zhang, Yun-Ming,et al."Nitrogen Starvation Induced Oxidative Stress in an Oil-Producing Green Alga Chlorella sorokiniana C3".PLOS ONE 8.7(2013):e69225.
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