| Nitrogen Starvation Induced Oxidative Stress in an Oil-Producing Green Alga Chlorella sorokiniana C3 | |
| Zhang, Yun-Ming1,2; Chen, Hui1; He, Chen-Liu1; Wang, Qiang1; Wang, Q (reprint author), Chinese Acad Sci, Key Lab Algal Biol, Inst Hydrobiol, Wuhan, Hubei Province, Peoples R China. | |
| 2013-07-16 | |
| Source Publication | PLOS ONE
 |
| ISSN | 1932-6203 |
| Volume | 8Issue:7Pages:e69225 |
| Abstract | Microalgal lipid is one of the most promising feedstocks for biodiesel production. Chlorella appears to be a particularly good option, and nitrogen (N) starvation is an efficient environmental pressure used to increase lipid accumulation in Chlorella cells. The effects of N starvation of an oil-producing wild microalga, Chlorella sorokiniana C3, on lipid accumulation were investigated using thin layer chromatography (TLC), confocal laser scanning microscopy (CLSM) and flow cytometry (FCM). The results showed that N starvation resulted in lipid accumulation in C. sorokiniana C3 cells, oil droplet (OD) formation and significant lipid accumulation in cells were detected after 2 d and 8 d of N starvation, respectively. During OD formation, reduced photosynthetic rate, respiration rate and photochemistry efficiency accompanied by increased damage to PSII were observed, demonstrated by chlorophyll (Chl) fluorescence, 77K fluorescence and oxygen evolution tests. In the mean time the rate of cyclic electron transportation increased correspondingly to produce more ATP for triacylglycerols (TAGs) synthesis. And 0.5 d was found to be the turning point for the early stress response and acclimation of cells to N starvation. Increased level of membrane peroxidation was also observed during OD formation, and superoxide dismutase (SOD), peroxide dismutase (POD) and catalase (CAT) enzyme activity assays suggested impaired reactive oxygen species (ROS) scavenging ability. Significant neutral lipid accumulation was also observed by artificial oxidative stress induced by H2O2 treatment. These results suggested coupled neutral lipid accumulation and oxidative stress during N starvation in C. sorokiniana C3.; Microalgal lipid is one of the most promising feedstocks for biodiesel production. Chlorella appears to be a particularly good option, and nitrogen (N) starvation is an efficient environmental pressure used to increase lipid accumulation in Chlorella cells. The effects of N starvation of an oil-producing wild microalga, Chlorella sorokiniana C3, on lipid accumulation were investigated using thin layer chromatography (TLC), confocal laser scanning microscopy (CLSM) and flow cytometry (FCM). The results showed that N starvation resulted in lipid accumulation in C. sorokiniana C3 cells, oil droplet (OD) formation and significant lipid accumulation in cells were detected after 2 d and 8 d of N starvation, respectively. During OD formation, reduced photosynthetic rate, respiration rate and photochemistry efficiency accompanied by increased damage to PSII were observed, demonstrated by chlorophyll (Chl) fluorescence, 77K fluorescence and oxygen evolution tests. In the mean time the rate of cyclic electron transportation increased correspondingly to produce more ATP for triacylglycerols (TAGs) synthesis. And 0.5 d was found to be the turning point for the early stress response and acclimation of cells to N starvation. Increased level of membrane peroxidation was also observed during OD formation, and superoxide dismutase (SOD), peroxide dismutase (POD) and catalase (CAT) enzyme activity assays suggested impaired reactive oxygen species (ROS) scavenging ability. Significant neutral lipid accumulation was also observed by artificial oxidative stress induced by H2O2 treatment. These results suggested coupled neutral lipid accumulation and oxidative stress during N starvation in C. sorokiniana C3. |
| Subtype | Article |
| Keyword | Biodiesel Production Lipid-accumulation Photosystem-i Microalgae Fluorescence Vulgaris Energy Light Antioxidants Carotenoids |
| Department | [Zhang, Yun-Ming ; Chen, Hui ; He, Chen-Liu ; Wang, Qiang] Chinese Acad Sci, Key Lab Algal Biol, Inst Hydrobiol, Wuhan, Hubei Province, Peoples R China ; [Zhang, Yun-Ming] Univ Chinese Acad Sci, Beijing, Peoples R China |
| DOI | 10.1371/journal.pone.0069225 |
| WOS Headings | Science & Technology |
| Funding Organization | National Program on Key Basic Research Project [2012CB224803]; Chinese Academy of Sciences [Y05101-1-601] ; National Program on Key Basic Research Project [2012CB224803]; Chinese Academy of Sciences [Y05101-1-601] ; National Program on Key Basic Research Project [2012CB224803]; Chinese Academy of Sciences [Y05101-1-601] ; National Program on Key Basic Research Project [2012CB224803]; Chinese Academy of Sciences [Y05101-1-601] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Science & Technology - Other Topics |
| WOS Subject | Multidisciplinary Sciences |
| WOS ID | WOS:000322064300093 |
| WOS Keyword | BIODIESEL PRODUCTION ; LIPID-ACCUMULATION ; PHOTOSYSTEM-I ; MICROALGAE ; FLUORESCENCE ; VULGARIS ; ENERGY ; LIGHT ; ANTIOXIDANTS ; CAROTENOIDS |
| Funding Organization | National Program on Key Basic Research Project [2012CB224803]; Chinese Academy of Sciences [Y05101-1-601] ; National Program on Key Basic Research Project [2012CB224803]; Chinese Academy of Sciences [Y05101-1-601] ; National Program on Key Basic Research Project [2012CB224803]; Chinese Academy of Sciences [Y05101-1-601] ; National Program on Key Basic Research Project [2012CB224803]; Chinese Academy of Sciences [Y05101-1-601] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/19632 |
| Collection | 藻类生物学及应用研究中心_期刊论文 |
| Corresponding Author | Wang, Q (reprint author), Chinese Acad Sci, Key Lab Algal Biol, Inst Hydrobiol, Wuhan, Hubei Province, Peoples R China. |
| Affiliation | 1.Chinese Acad Sci, Key Lab Algal Biol, Inst Hydrobiol, Wuhan, Hubei Province, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| Recommended Citation GB/T 7714 | Zhang, Yun-Ming,Chen, Hui,He, Chen-Liu,et al. Nitrogen Starvation Induced Oxidative Stress in an Oil-Producing Green Alga Chlorella sorokiniana C3[J]. PLOS ONE,2013,8(7):e69225. |
| APA | Zhang, Yun-Ming,Chen, Hui,He, Chen-Liu,Wang, Qiang,&Wang, Q .(2013).Nitrogen Starvation Induced Oxidative Stress in an Oil-Producing Green Alga Chlorella sorokiniana C3.PLOS ONE,8(7),e69225. |
| MLA | Zhang, Yun-Ming,et al."Nitrogen Starvation Induced Oxidative Stress in an Oil-Producing Green Alga Chlorella sorokiniana C3".PLOS ONE 8.7(2013):e69225. |
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