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Zebrafish locomotor capacity and brain acetylcholinesterase activity is altered by Aphanizomenon flos-aquae DC-1 aphantoxins
Zhang, De Lu1,2; Hu, Chun Xiang2; Li, Dun Hai2; Liu, Yong Ding2; Hu, CX (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
2013-08-15
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume138Pages:139-149
AbstractAphanizomenon flos-aquae (A. flos-aquae) is a source of neurotoxins known as aphantoxins or paralytic shellfish poisons (PSPs) that present a major threat to the environment and to human health. Generally, altered neurological function is reflected in behavior. Although the molecular mechanism of action of PSPs is well known, its neurobehavioral effects on adult zebrafish and its relationship with altered neurological functions are poorly understood. Aphantoxins purified from a natural isolate of A. flos-aquae DC-1 were analyzed by HPLC. The major analogs found in the toxins were the gonyautoxins 1 and 5 (GTX1 and GTX5; 34.04% and 21.28%, respectively) and the neosaxitoxin (neoSTX, 12.77%). Zebrafish (Danio rerio) were intraperitoneally injected with 5.3 and 7.61 mu g STXeq/kg (low and high dose, respectively) of A. flos-aquae DC-1 aphantoxins. The swimming activity was investigated by observation combined with video at 6 timepoints from 1 to 24 h post-exposure. Both aphantoxin doses were associated with delayed touch responses, reduced head tail locomotory abilities, inflexible turning of head, and a tailward-shifted center of gravity. The normal S-pattern (or undulating) locomotor trajectory was replaced by a mechanical motor pattern of swinging the head after wagging the tail. Finally, these fish principally distributed at the top and/or bottom water of the aquarium, and showed a clear polarized distribution pattern at 12 h post-exposure. Further analysis of neurological function demonstrated that both aphantoxin doses inhibited brain acetylcholinesterase activity. All these changes were dose- and time-dependent. These results demonstrate that aphantoxins can alter locomotor capacity, touch responses and distribution patterns by damaging the cholinergic system of zebrafish, and suggest that zebrafish locomotor behavior and acetylcholinesterase can be used as indicators for investigating aphantoxins and blooms in nature. Published by Elsevier B.V.; Aphanizomenon flos-aquae (A. flos-aquae) is a source of neurotoxins known as aphantoxins or paralytic shellfish poisons (PSPs) that present a major threat to the environment and to human health. Generally, altered neurological function is reflected in behavior. Although the molecular mechanism of action of PSPs is well known, its neurobehavioral effects on adult zebrafish and its relationship with altered neurological functions are poorly understood. Aphantoxins purified from a natural isolate of A. flos-aquae DC-1 were analyzed by HPLC. The major analogs found in the toxins were the gonyautoxins 1 and 5 (GTX1 and GTX5; 34.04% and 21.28%, respectively) and the neosaxitoxin (neoSTX, 12.77%). Zebrafish (Danio rerio) were intraperitoneally injected with 5.3 and 7.61 mu g STXeq/kg (low and high dose, respectively) of A. flos-aquae DC-1 aphantoxins. The swimming activity was investigated by observation combined with video at 6 timepoints from 1 to 24 h post-exposure. Both aphantoxin doses were associated with delayed touch responses, reduced head tail locomotory abilities, inflexible turning of head, and a tailward-shifted center of gravity. The normal S-pattern (or undulating) locomotor trajectory was replaced by a mechanical motor pattern of swinging the head after wagging the tail. Finally, these fish principally distributed at the top and/or bottom water of the aquarium, and showed a clear polarized distribution pattern at 12 h post-exposure. Further analysis of neurological function demonstrated that both aphantoxin doses inhibited brain acetylcholinesterase activity. All these changes were dose- and time-dependent. These results demonstrate that aphantoxins can alter locomotor capacity, touch responses and distribution patterns by damaging the cholinergic system of zebrafish, and suggest that zebrafish locomotor behavior and acetylcholinesterase can be used as indicators for investigating aphantoxins and blooms in nature. Published by Elsevier B.V.
SubtypeArticle
KeywordAphantoxins Mechanosensory Change Locomotor Abnormality Acetylcholinesterase Brain Zebrafish
Department[Zhang, De Lu] Wuhan Univ Technol, Coll Sci, Dept Lifesci & Biotechnol, Wuhan 430070, Peoples R China ; [Zhang, De Lu ; Hu, Chun Xiang ; Li, Dun Hai ; Liu, Yong Ding] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
DOI10.1016/j.aquatox.2013.04.016
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Basic Research Program of China [2008CB418001]; National High-tech Research and Development Program of China [2013AA065804] ; National Basic Research Program of China [2008CB418001]; National High-tech Research and Development Program of China [2013AA065804] ; National Basic Research Program of China [2008CB418001]; National High-tech Research and Development Program of China [2013AA065804] ; National Basic Research Program of China [2008CB418001]; National High-tech Research and Development Program of China [2013AA065804]
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology ; Toxicology
WOS SubjectMarine & Freshwater Biology ; Toxicology
WOS IDWOS:000322293600016
WOS KeywordPARALYTIC SHELLFISH TOXINS ; INDUCED BEHAVIORAL-CHANGES ; TROUT ONCORHYNCHUS-MYKISS ; HARMFUL ALGAL BLOOMS ; DANIO-RERIO ; GYMNODINIUM-CATENATUM ; SWIMMING ACTIVITY ; ETHANOL EXPOSURE ; POISONING TOXINS ; ADULT ZEBRAFISH
Funding OrganizationNational Basic Research Program of China [2008CB418001]; National High-tech Research and Development Program of China [2013AA065804] ; National Basic Research Program of China [2008CB418001]; National High-tech Research and Development Program of China [2013AA065804] ; National Basic Research Program of China [2008CB418001]; National High-tech Research and Development Program of China [2013AA065804] ; National Basic Research Program of China [2008CB418001]; National High-tech Research and Development Program of China [2013AA065804]
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19628
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorHu, CX (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
Affiliation1.Wuhan Univ Technol, Coll Sci, Dept Lifesci & Biotechnol, Wuhan 430070, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhang, De Lu,Hu, Chun Xiang,Li, Dun Hai,et al. Zebrafish locomotor capacity and brain acetylcholinesterase activity is altered by Aphanizomenon flos-aquae DC-1 aphantoxins[J]. AQUATIC TOXICOLOGY,2013,138:139-149.
APA Zhang, De Lu,Hu, Chun Xiang,Li, Dun Hai,Liu, Yong Ding,&Hu, CX .(2013).Zebrafish locomotor capacity and brain acetylcholinesterase activity is altered by Aphanizomenon flos-aquae DC-1 aphantoxins.AQUATIC TOXICOLOGY,138,139-149.
MLA Zhang, De Lu,et al."Zebrafish locomotor capacity and brain acetylcholinesterase activity is altered by Aphanizomenon flos-aquae DC-1 aphantoxins".AQUATIC TOXICOLOGY 138(2013):139-149.
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