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题名: The divergence of cpcBA-IGS sequences between Dolichospermum and Aphanizomenon (Cyanobacteria) and the molecular detection of Dolichospermum flos-aquae in Taihu Lake, China
作者: Wang, Zhongjie1, 2; Liu, Yang1, 2; Xu, Yao1; Xiao, Peng1, 2; Li, Renhui1
通讯作者: Li, RH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China.
关键词: cpcBA-IGS ; Cyanobacteria ; Dolichospermum flos-aquae ; Real-time PCR ; Taihu Lake
刊名: PHYCOLOGIA
发表日期: 2013-09-01
DOI: 10.2216/12-125.1
卷: 52, 期:5, 页:447-454
收录类别: SCI
文章类型: Article
部门归属: [Wang, Zhongjie ; Liu, Yang ; Xu, Yao ; Xiao, Peng ; Li, Renhui] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China ; [Wang, Zhongjie ; Liu, Yang ; Xiao, Peng] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: NSFC-JST joint project [41261140337]; State Key Basic Research and Development Plan of China [2008CB418002]
类目[WOS]: Plant Sciences ; Marine & Freshwater Biology
研究领域[WOS]: Plant Sciences ; Marine & Freshwater Biology
摘要: The occurrence of bloom-forming heterocystous cyanobacteria such as Dolichospermum flos-aquae has become increasingly serious. This study was conducted to provide a detailed understanding of diversity, distribution and seasonal dynamics of Dolichospermum flos-aquae populations in Taihu Lake, China. Morphological identifications showed Dolichospermum flos-aquae and Aphanizomnon flos-aquae were the two main heterocystous cyanobacteria in Taihu Lake. Phylogenetic analysis based on cpcBA-IGS sequences, the highly variable intergenic spacer (IGS) region between two phycobilisome subunits (cpcB and cpcA), distinguished Dolichospermum from other filametous cyanobacterial genera. Therefore, the real-time PCR detection of Dolichospermum flos-aquae based on cpcBA-IGS sequences was developed to study the distribution and abundance of Dolichospermum flos-aquae. The investigation began in August 2008 and extended through September 2010. Dolichospermum flos-aquae occurred throughout the entire lake. There was an average abundance of 2.0 x 10(6) gene copies L-1, and the peak abundance was in the western part of Taihu Lake during late spring when it reached 4.0 x 10(7) copies L-1. Dolichospermum flos-aquae had a regular annual cycle; it began appearing in late autumn and it reached a peak in late spring.
英文摘要: The occurrence of bloom-forming heterocystous cyanobacteria such as Dolichospermum flos-aquae has become increasingly serious. This study was conducted to provide a detailed understanding of diversity, distribution and seasonal dynamics of Dolichospermum flos-aquae populations in Taihu Lake, China. Morphological identifications showed Dolichospermum flos-aquae and Aphanizomnon flos-aquae were the two main heterocystous cyanobacteria in Taihu Lake. Phylogenetic analysis based on cpcBA-IGS sequences, the highly variable intergenic spacer (IGS) region between two phycobilisome subunits (cpcB and cpcA), distinguished Dolichospermum from other filametous cyanobacterial genera. Therefore, the real-time PCR detection of Dolichospermum flos-aquae based on cpcBA-IGS sequences was developed to study the distribution and abundance of Dolichospermum flos-aquae. The investigation began in August 2008 and extended through September 2010. Dolichospermum flos-aquae occurred throughout the entire lake. There was an average abundance of 2.0 x 10(6) gene copies L-1, and the peak abundance was in the western part of Taihu Lake during late spring when it reached 4.0 x 10(7) copies L-1. Dolichospermum flos-aquae had a regular annual cycle; it began appearing in late autumn and it reached a peak in late spring.
关键词[WOS]: REAL-TIME PCR ; LARGE SHALLOW LAKE ; ENVIRONMENTAL-FACTORS ; MICROCYSTIS BLOOMS ; ANATOXIN-A ; ANABAENA ; WATER ; PHOSPHORUS ; TOXICITY
语种: 英语
WOS记录号: WOS:000325965100007
ISSN号: 0031-8884
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19625
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Wang, Zhongjie; Liu, Yang; Xu, Yao; Xiao, Peng; Li, Renhui.The divergence of cpcBA-IGS sequences between Dolichospermum and Aphanizomenon (Cyanobacteria) and the molecular detection of Dolichospermum flos-aquae in Taihu Lake, China,PHYCOLOGIA,2013,52(5):447-454
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