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The divergence of cpcBA-IGS sequences between Dolichospermum and Aphanizomenon (Cyanobacteria) and the molecular detection of Dolichospermum flos-aquae in Taihu Lake, China
Wang, Zhongjie1,2; Liu, Yang1,2; Xu, Yao1; Xiao, Peng1,2; Li, Renhui1; Li, RH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China.
2013-09-01
Source PublicationPHYCOLOGIA
ISSN0031-8884
Volume52Issue:5Pages:447-454
AbstractThe occurrence of bloom-forming heterocystous cyanobacteria such as Dolichospermum flos-aquae has become increasingly serious. This study was conducted to provide a detailed understanding of diversity, distribution and seasonal dynamics of Dolichospermum flos-aquae populations in Taihu Lake, China. Morphological identifications showed Dolichospermum flos-aquae and Aphanizomnon flos-aquae were the two main heterocystous cyanobacteria in Taihu Lake. Phylogenetic analysis based on cpcBA-IGS sequences, the highly variable intergenic spacer (IGS) region between two phycobilisome subunits (cpcB and cpcA), distinguished Dolichospermum from other filametous cyanobacterial genera. Therefore, the real-time PCR detection of Dolichospermum flos-aquae based on cpcBA-IGS sequences was developed to study the distribution and abundance of Dolichospermum flos-aquae. The investigation began in August 2008 and extended through September 2010. Dolichospermum flos-aquae occurred throughout the entire lake. There was an average abundance of 2.0 x 10(6) gene copies L-1, and the peak abundance was in the western part of Taihu Lake during late spring when it reached 4.0 x 10(7) copies L-1. Dolichospermum flos-aquae had a regular annual cycle; it began appearing in late autumn and it reached a peak in late spring.; The occurrence of bloom-forming heterocystous cyanobacteria such as Dolichospermum flos-aquae has become increasingly serious. This study was conducted to provide a detailed understanding of diversity, distribution and seasonal dynamics of Dolichospermum flos-aquae populations in Taihu Lake, China. Morphological identifications showed Dolichospermum flos-aquae and Aphanizomnon flos-aquae were the two main heterocystous cyanobacteria in Taihu Lake. Phylogenetic analysis based on cpcBA-IGS sequences, the highly variable intergenic spacer (IGS) region between two phycobilisome subunits (cpcB and cpcA), distinguished Dolichospermum from other filametous cyanobacterial genera. Therefore, the real-time PCR detection of Dolichospermum flos-aquae based on cpcBA-IGS sequences was developed to study the distribution and abundance of Dolichospermum flos-aquae. The investigation began in August 2008 and extended through September 2010. Dolichospermum flos-aquae occurred throughout the entire lake. There was an average abundance of 2.0 x 10(6) gene copies L-1, and the peak abundance was in the western part of Taihu Lake during late spring when it reached 4.0 x 10(7) copies L-1. Dolichospermum flos-aquae had a regular annual cycle; it began appearing in late autumn and it reached a peak in late spring.
SubtypeArticle
KeywordCpcba-igs Cyanobacteria Dolichospermum Flos-aquae Real-time Pcr Taihu Lake
Department[Wang, Zhongjie ; Liu, Yang ; Xu, Yao ; Xiao, Peng ; Li, Renhui] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China ; [Wang, Zhongjie ; Liu, Yang ; Xiao, Peng] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
DOI10.2216/12-125.1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNSFC-JST joint project [41261140337]; State Key Basic Research and Development Plan of China [2008CB418002] ; NSFC-JST joint project [41261140337]; State Key Basic Research and Development Plan of China [2008CB418002] ; NSFC-JST joint project [41261140337]; State Key Basic Research and Development Plan of China [2008CB418002] ; NSFC-JST joint project [41261140337]; State Key Basic Research and Development Plan of China [2008CB418002]
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences ; Marine & Freshwater Biology
WOS SubjectPlant Sciences ; Marine & Freshwater Biology
WOS IDWOS:000325965100007
WOS KeywordREAL-TIME PCR ; LARGE SHALLOW LAKE ; ENVIRONMENTAL-FACTORS ; MICROCYSTIS BLOOMS ; ANATOXIN-A ; ANABAENA ; WATER ; PHOSPHORUS ; TOXICITY
Funding OrganizationNSFC-JST joint project [41261140337]; State Key Basic Research and Development Plan of China [2008CB418002] ; NSFC-JST joint project [41261140337]; State Key Basic Research and Development Plan of China [2008CB418002] ; NSFC-JST joint project [41261140337]; State Key Basic Research and Development Plan of China [2008CB418002] ; NSFC-JST joint project [41261140337]; State Key Basic Research and Development Plan of China [2008CB418002]
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19625
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorLi, RH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wang, Zhongjie,Liu, Yang,Xu, Yao,et al. The divergence of cpcBA-IGS sequences between Dolichospermum and Aphanizomenon (Cyanobacteria) and the molecular detection of Dolichospermum flos-aquae in Taihu Lake, China[J]. PHYCOLOGIA,2013,52(5):447-454.
APA Wang, Zhongjie,Liu, Yang,Xu, Yao,Xiao, Peng,Li, Renhui,&Li, RH .(2013).The divergence of cpcBA-IGS sequences between Dolichospermum and Aphanizomenon (Cyanobacteria) and the molecular detection of Dolichospermum flos-aquae in Taihu Lake, China.PHYCOLOGIA,52(5),447-454.
MLA Wang, Zhongjie,et al."The divergence of cpcBA-IGS sequences between Dolichospermum and Aphanizomenon (Cyanobacteria) and the molecular detection of Dolichospermum flos-aquae in Taihu Lake, China".PHYCOLOGIA 52.5(2013):447-454.
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