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题名: UVB Radiation as a Potential Selective Factor Favoring Microcystin Producing Bloom Forming Cyanobacteria
作者: Ding, Yi1, 2; Song, Lirong1; Sedmak, Bojan3
通讯作者: Sedmak, B (reprint author), Natl Inst Biol, Dept Genet Toxicol & Canc Biol, Ljubljana, Slovenia.
关键词: ULTRAVIOLET-RADIATION ; LIGHT ; AERUGINOSA ; SOLAR ; BIOSYNTHESIS ; EVOLUTION ; GROWTH ; DAMAGE ; GENES
刊名: PLOS ONE
发表日期: 2013-09-13
DOI: 10.1371/journal.pone.0073919
卷: 8, 期:9, 页:e73919
收录类别: SCI
文章类型: Article
部门归属: [Ding, Yi ; Song, Lirong] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Peoples R China ; [Ding, Yi] Univ Chinese Acad Sci, Beijing, Peoples R China ; [Sedmak, Bojan] Natl Inst Biol, Dept Genet Toxicol & Canc Biol, Ljubljana, Slovenia
WOS标题词: Science & Technology
资助者: Slovenian Research Agency
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
摘要: Due to the stratospheric ozone depletion, several organisms will become exposed to increased biologically active UVB (280-320nm) radiation, not only at polar but also at temperate and tropical latitudes. Bloom forming cyanobacteria are exposed to UVB radiation on a mass scale, particularly during the surface bloom and scum formation that can persist for long periods of time. All buoyant species of cyanobacteria are at least periodically exposed to higher irradiation during their vertical migration to the surface that usually occurs several times a day. The aim of this study is to assess the influence on cyanobacteria of UVB radiation at realistic environmental intensities. The effects of two UVB intensities of 0.5 and 0.99 W/m(2) in up to 0.5 cm water depth were studied in vitro on Microcystis aeruginosa strains, two microcystin producing and one non-producing. After UVB exposure their ability to proliferate was estimated by cell counting, while cell fitness and integrity were evaluated using light microscopy, autofluorescence and immunofluorescence. Gene damage was assessed by TUNEL assay and SYBR Green staining of the nucleoide area. We conclude that UVB exposure causes damage to the genetic material, cytoskeletal elements, higher sedimentation rates and consequent cell death. In contrast to microcystin producers (PCC7806 and FACHB905), the microcystin non-producing strain PCC7005 is more susceptible to the deleterious effects of radiation, with weak recovery ability. The ecological relevance of the results is discussed using data from eleven years' continuous UVB radiation measurements within the area of Ljubljana city (Slovenia, Central Europe). Our results suggest that increased solar radiation in temperate latitudes can have its strongest effect during cyanobacterial bloom formation in spring and early summer. UVB radiation in this period may significantly influence strain composition of cyanobacterial blooms in favor of microcystin producers.
英文摘要: Due to the stratospheric ozone depletion, several organisms will become exposed to increased biologically active UVB (280-320nm) radiation, not only at polar but also at temperate and tropical latitudes. Bloom forming cyanobacteria are exposed to UVB radiation on a mass scale, particularly during the surface bloom and scum formation that can persist for long periods of time. All buoyant species of cyanobacteria are at least periodically exposed to higher irradiation during their vertical migration to the surface that usually occurs several times a day. The aim of this study is to assess the influence on cyanobacteria of UVB radiation at realistic environmental intensities. The effects of two UVB intensities of 0.5 and 0.99 W/m(2) in up to 0.5 cm water depth were studied in vitro on Microcystis aeruginosa strains, two microcystin producing and one non-producing. After UVB exposure their ability to proliferate was estimated by cell counting, while cell fitness and integrity were evaluated using light microscopy, autofluorescence and immunofluorescence. Gene damage was assessed by TUNEL assay and SYBR Green staining of the nucleoide area. We conclude that UVB exposure causes damage to the genetic material, cytoskeletal elements, higher sedimentation rates and consequent cell death. In contrast to microcystin producers (PCC7806 and FACHB905), the microcystin non-producing strain PCC7005 is more susceptible to the deleterious effects of radiation, with weak recovery ability. The ecological relevance of the results is discussed using data from eleven years' continuous UVB radiation measurements within the area of Ljubljana city (Slovenia, Central Europe). Our results suggest that increased solar radiation in temperate latitudes can have its strongest effect during cyanobacterial bloom formation in spring and early summer. UVB radiation in this period may significantly influence strain composition of cyanobacterial blooms in favor of microcystin producers.
关键词[WOS]: ULTRAVIOLET-RADIATION ; LIGHT ; AERUGINOSA ; SOLAR ; BIOSYNTHESIS ; EVOLUTION ; GROWTH ; DAMAGE ; GENES
语种: 英语
WOS记录号: WOS:000324408400043
ISSN号: 1932-6203
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19624
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Natl Inst Biol, Dept Genet Toxicol & Canc Biol, Ljubljana, Slovenia

Recommended Citation:
Ding, Yi; Song, Lirong; Sedmak, Bojan.UVB Radiation as a Potential Selective Factor Favoring Microcystin Producing Bloom Forming Cyanobacteria,PLOS ONE,2013,8(9):e73919
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