IHB OpenIR  > 藻类生物学及应用研究中心  > 期刊论文
UVB Radiation as a Potential Selective Factor Favoring Microcystin Producing Bloom Forming Cyanobacteria
Ding, Yi1,2; Song, Lirong1; Sedmak, Bojan3; Sedmak, B (reprint author), Natl Inst Biol, Dept Genet Toxicol & Canc Biol, Ljubljana, Slovenia.
2013-09-13
Source PublicationPLOS ONE
ISSN1932-6203
Volume8Issue:9Pages:e73919
AbstractDue to the stratospheric ozone depletion, several organisms will become exposed to increased biologically active UVB (280-320nm) radiation, not only at polar but also at temperate and tropical latitudes. Bloom forming cyanobacteria are exposed to UVB radiation on a mass scale, particularly during the surface bloom and scum formation that can persist for long periods of time. All buoyant species of cyanobacteria are at least periodically exposed to higher irradiation during their vertical migration to the surface that usually occurs several times a day. The aim of this study is to assess the influence on cyanobacteria of UVB radiation at realistic environmental intensities. The effects of two UVB intensities of 0.5 and 0.99 W/m(2) in up to 0.5 cm water depth were studied in vitro on Microcystis aeruginosa strains, two microcystin producing and one non-producing. After UVB exposure their ability to proliferate was estimated by cell counting, while cell fitness and integrity were evaluated using light microscopy, autofluorescence and immunofluorescence. Gene damage was assessed by TUNEL assay and SYBR Green staining of the nucleoide area. We conclude that UVB exposure causes damage to the genetic material, cytoskeletal elements, higher sedimentation rates and consequent cell death. In contrast to microcystin producers (PCC7806 and FACHB905), the microcystin non-producing strain PCC7005 is more susceptible to the deleterious effects of radiation, with weak recovery ability. The ecological relevance of the results is discussed using data from eleven years' continuous UVB radiation measurements within the area of Ljubljana city (Slovenia, Central Europe). Our results suggest that increased solar radiation in temperate latitudes can have its strongest effect during cyanobacterial bloom formation in spring and early summer. UVB radiation in this period may significantly influence strain composition of cyanobacterial blooms in favor of microcystin producers.; Due to the stratospheric ozone depletion, several organisms will become exposed to increased biologically active UVB (280-320nm) radiation, not only at polar but also at temperate and tropical latitudes. Bloom forming cyanobacteria are exposed to UVB radiation on a mass scale, particularly during the surface bloom and scum formation that can persist for long periods of time. All buoyant species of cyanobacteria are at least periodically exposed to higher irradiation during their vertical migration to the surface that usually occurs several times a day. The aim of this study is to assess the influence on cyanobacteria of UVB radiation at realistic environmental intensities. The effects of two UVB intensities of 0.5 and 0.99 W/m(2) in up to 0.5 cm water depth were studied in vitro on Microcystis aeruginosa strains, two microcystin producing and one non-producing. After UVB exposure their ability to proliferate was estimated by cell counting, while cell fitness and integrity were evaluated using light microscopy, autofluorescence and immunofluorescence. Gene damage was assessed by TUNEL assay and SYBR Green staining of the nucleoide area. We conclude that UVB exposure causes damage to the genetic material, cytoskeletal elements, higher sedimentation rates and consequent cell death. In contrast to microcystin producers (PCC7806 and FACHB905), the microcystin non-producing strain PCC7005 is more susceptible to the deleterious effects of radiation, with weak recovery ability. The ecological relevance of the results is discussed using data from eleven years' continuous UVB radiation measurements within the area of Ljubljana city (Slovenia, Central Europe). Our results suggest that increased solar radiation in temperate latitudes can have its strongest effect during cyanobacterial bloom formation in spring and early summer. UVB radiation in this period may significantly influence strain composition of cyanobacterial blooms in favor of microcystin producers.
SubtypeArticle
KeywordUltraviolet-radiation Light Aeruginosa Solar Biosynthesis Evolution Growth Damage Genes
Department[Ding, Yi ; Song, Lirong] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Peoples R China ; [Ding, Yi] Univ Chinese Acad Sci, Beijing, Peoples R China ; [Sedmak, Bojan] Natl Inst Biol, Dept Genet Toxicol & Canc Biol, Ljubljana, Slovenia
DOI10.1371/journal.pone.0073919
WOS HeadingsScience & Technology
Funding OrganizationSlovenian Research Agency ; Slovenian Research Agency ; Slovenian Research Agency ; Slovenian Research Agency
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000324408400043
WOS KeywordULTRAVIOLET-RADIATION ; LIGHT ; AERUGINOSA ; SOLAR ; BIOSYNTHESIS ; EVOLUTION ; GROWTH ; DAMAGE ; GENES
Funding OrganizationSlovenian Research Agency ; Slovenian Research Agency ; Slovenian Research Agency ; Slovenian Research Agency
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19624
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorSedmak, B (reprint author), Natl Inst Biol, Dept Genet Toxicol & Canc Biol, Ljubljana, Slovenia.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Natl Inst Biol, Dept Genet Toxicol & Canc Biol, Ljubljana, Slovenia
Recommended Citation
GB/T 7714
Ding, Yi,Song, Lirong,Sedmak, Bojan,et al. UVB Radiation as a Potential Selective Factor Favoring Microcystin Producing Bloom Forming Cyanobacteria[J]. PLOS ONE,2013,8(9):e73919.
APA Ding, Yi,Song, Lirong,Sedmak, Bojan,&Sedmak, B .(2013).UVB Radiation as a Potential Selective Factor Favoring Microcystin Producing Bloom Forming Cyanobacteria.PLOS ONE,8(9),e73919.
MLA Ding, Yi,et al."UVB Radiation as a Potential Selective Factor Favoring Microcystin Producing Bloom Forming Cyanobacteria".PLOS ONE 8.9(2013):e73919.
Files in This Item:
File Name/Size DocType Version Access License
UVB Radiation as a P(1128KB) 开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Ding, Yi]'s Articles
[Song, Lirong]'s Articles
[Sedmak, Bojan]'s Articles
Baidu academic
Similar articles in Baidu academic
[Ding, Yi]'s Articles
[Song, Lirong]'s Articles
[Sedmak, Bojan]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Ding, Yi]'s Articles
[Song, Lirong]'s Articles
[Sedmak, Bojan]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: UVB Radiation as a Potential Selective Factor Favoring Microcystin Producing Bloom Forming Cyanobacteria.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.