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Congruence between mcy based genetic type and microcystin composition within the populations of toxic Microcystis in a plateau lake, China
Jiang, Yongguang1,2; Yu, Gongliang1; Chai, Wenbo3; Song, Gaofei1,2; Li, Renhui1; Li, RH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China.
2013-10-01
Source PublicationENVIRONMENTAL MICROBIOLOGY REPORTS
ISSN1758-2229
Volume5Issue:5Pages:637-647
AbstractGenetic diversity and differential microcystin contributions within the populations of toxic Microcystis in freshwater ecosystems have not been fully discussed. To illustrate this issue, we sequenced clone libraries of the first adenylation domain of mcyB (mcyBA1) from Microcystis populations in a plateau lake. Phylogenetic analysis revealed two divergent groups of McyBA1 with two closely related subgroups within Group I. While neutral evolution was indicated on the whole McyBA1 domain, 1-5 recombination breakpoints and several codons under positive or negative selections were found. Significant seasonal changes of McyBA1 diversity were observed. Group I composed the major proportion of the McyBA1 pool throughout the growing season in the whole lake. Group IA and Group II denominated Microcystis strains isolated from this lake were characterized by preference production of microcystin-RR (62-85%) and microcystin-LR (>98%) respectively. We detected the intracellular microcystins in lake water and microcystin-RR was a main variant (mostly>50%). In summary, McyBA1 subgroups were dominant within the population of toxic Microcystis and contributed the predominance of microcystin-RR in the lake. The differences of substrate preference in microcystin biosynthesis among groups were caused by neutral evolution and homologous recombination.; Genetic diversity and differential microcystin contributions within the populations of toxic Microcystis in freshwater ecosystems have not been fully discussed. To illustrate this issue, we sequenced clone libraries of the first adenylation domain of mcyB (mcyBA1) from Microcystis populations in a plateau lake. Phylogenetic analysis revealed two divergent groups of McyBA1 with two closely related subgroups within Group I. While neutral evolution was indicated on the whole McyBA1 domain, 1-5 recombination breakpoints and several codons under positive or negative selections were found. Significant seasonal changes of McyBA1 diversity were observed. Group I composed the major proportion of the McyBA1 pool throughout the growing season in the whole lake. Group IA and Group II denominated Microcystis strains isolated from this lake were characterized by preference production of microcystin-RR (62-85%) and microcystin-LR (>98%) respectively. We detected the intracellular microcystins in lake water and microcystin-RR was a main variant (mostly>50%). In summary, McyBA1 subgroups were dominant within the population of toxic Microcystis and contributed the predominance of microcystin-RR in the lake. The differences of substrate preference in microcystin biosynthesis among groups were caused by neutral evolution and homologous recombination.
SubtypeArticle
KeywordMaximum-likelihood Environmental-factors Adenylation Domains Positive Selection Cyanobacteria Aeruginosa Diversity Blooms Water Biosynthesis
Department[Jiang, Yongguang ; Yu, Gongliang ; Song, Gaofei ; Li, Renhui] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China ; [Jiang, Yongguang ; Song, Gaofei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Chai, Wenbo] Jiangxi Normal Univ, Sch Life Sci, Nanchang 330022, Peoples R China
DOI10.1111/1758-2229.12062
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Water Science and Technology Projects [2012ZX07105-004]; National Natural Science Foundation of China [31170189]; Talent Scientist Program of the Chinese Academy of Sciences [082303-1-501] ; National Water Science and Technology Projects [2012ZX07105-004]; National Natural Science Foundation of China [31170189]; Talent Scientist Program of the Chinese Academy of Sciences [082303-1-501] ; National Water Science and Technology Projects [2012ZX07105-004]; National Natural Science Foundation of China [31170189]; Talent Scientist Program of the Chinese Academy of Sciences [082303-1-501] ; National Water Science and Technology Projects [2012ZX07105-004]; National Natural Science Foundation of China [31170189]; Talent Scientist Program of the Chinese Academy of Sciences [082303-1-501]
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Microbiology
WOS SubjectEnvironmental Sciences ; Microbiology
WOS IDWOS:000325142700002
WOS KeywordMAXIMUM-LIKELIHOOD ; ENVIRONMENTAL-FACTORS ; ADENYLATION DOMAINS ; POSITIVE SELECTION ; CYANOBACTERIA ; AERUGINOSA ; DIVERSITY ; BLOOMS ; WATER ; BIOSYNTHESIS
Funding OrganizationNational Water Science and Technology Projects [2012ZX07105-004]; National Natural Science Foundation of China [31170189]; Talent Scientist Program of the Chinese Academy of Sciences [082303-1-501] ; National Water Science and Technology Projects [2012ZX07105-004]; National Natural Science Foundation of China [31170189]; Talent Scientist Program of the Chinese Academy of Sciences [082303-1-501] ; National Water Science and Technology Projects [2012ZX07105-004]; National Natural Science Foundation of China [31170189]; Talent Scientist Program of the Chinese Academy of Sciences [082303-1-501] ; National Water Science and Technology Projects [2012ZX07105-004]; National Natural Science Foundation of China [31170189]; Talent Scientist Program of the Chinese Academy of Sciences [082303-1-501]
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Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19618
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorLi, RH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Jiangxi Normal Univ, Sch Life Sci, Nanchang 330022, Peoples R China
Recommended Citation
GB/T 7714
Jiang, Yongguang,Yu, Gongliang,Chai, Wenbo,et al. Congruence between mcy based genetic type and microcystin composition within the populations of toxic Microcystis in a plateau lake, China[J]. ENVIRONMENTAL MICROBIOLOGY REPORTS,2013,5(5):637-647.
APA Jiang, Yongguang,Yu, Gongliang,Chai, Wenbo,Song, Gaofei,Li, Renhui,&Li, RH .(2013).Congruence between mcy based genetic type and microcystin composition within the populations of toxic Microcystis in a plateau lake, China.ENVIRONMENTAL MICROBIOLOGY REPORTS,5(5),637-647.
MLA Jiang, Yongguang,et al."Congruence between mcy based genetic type and microcystin composition within the populations of toxic Microcystis in a plateau lake, China".ENVIRONMENTAL MICROBIOLOGY REPORTS 5.5(2013):637-647.
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