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题名: Congruence between mcy based genetic type and microcystin composition within the populations of toxic Microcystis in a plateau lake, China
作者: Jiang, Yongguang1, 2; Yu, Gongliang1; Chai, Wenbo3; Song, Gaofei1, 2; Li, Renhui1
通讯作者: Li, RH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China.
关键词: MAXIMUM-LIKELIHOOD ; ENVIRONMENTAL-FACTORS ; ADENYLATION DOMAINS ; POSITIVE SELECTION ; CYANOBACTERIA ; AERUGINOSA ; DIVERSITY ; BLOOMS ; WATER ; BIOSYNTHESIS
刊名: ENVIRONMENTAL MICROBIOLOGY REPORTS
发表日期: 2013-10-01
DOI: 10.1111/1758-2229.12062
卷: 5, 期:5, 页:637-647
收录类别: SCI
文章类型: Article
部门归属: [Jiang, Yongguang ; Yu, Gongliang ; Song, Gaofei ; Li, Renhui] Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China ; [Jiang, Yongguang ; Song, Gaofei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Chai, Wenbo] Jiangxi Normal Univ, Sch Life Sci, Nanchang 330022, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Water Science and Technology Projects [2012ZX07105-004]; National Natural Science Foundation of China [31170189]; Talent Scientist Program of the Chinese Academy of Sciences [082303-1-501]
类目[WOS]: Environmental Sciences ; Microbiology
研究领域[WOS]: Environmental Sciences & Ecology ; Microbiology
摘要: Genetic diversity and differential microcystin contributions within the populations of toxic Microcystis in freshwater ecosystems have not been fully discussed. To illustrate this issue, we sequenced clone libraries of the first adenylation domain of mcyB (mcyBA1) from Microcystis populations in a plateau lake. Phylogenetic analysis revealed two divergent groups of McyBA1 with two closely related subgroups within Group I. While neutral evolution was indicated on the whole McyBA1 domain, 1-5 recombination breakpoints and several codons under positive or negative selections were found. Significant seasonal changes of McyBA1 diversity were observed. Group I composed the major proportion of the McyBA1 pool throughout the growing season in the whole lake. Group IA and Group II denominated Microcystis strains isolated from this lake were characterized by preference production of microcystin-RR (62-85%) and microcystin-LR (>98%) respectively. We detected the intracellular microcystins in lake water and microcystin-RR was a main variant (mostly>50%). In summary, McyBA1 subgroups were dominant within the population of toxic Microcystis and contributed the predominance of microcystin-RR in the lake. The differences of substrate preference in microcystin biosynthesis among groups were caused by neutral evolution and homologous recombination.
英文摘要: Genetic diversity and differential microcystin contributions within the populations of toxic Microcystis in freshwater ecosystems have not been fully discussed. To illustrate this issue, we sequenced clone libraries of the first adenylation domain of mcyB (mcyBA1) from Microcystis populations in a plateau lake. Phylogenetic analysis revealed two divergent groups of McyBA1 with two closely related subgroups within Group I. While neutral evolution was indicated on the whole McyBA1 domain, 1-5 recombination breakpoints and several codons under positive or negative selections were found. Significant seasonal changes of McyBA1 diversity were observed. Group I composed the major proportion of the McyBA1 pool throughout the growing season in the whole lake. Group IA and Group II denominated Microcystis strains isolated from this lake were characterized by preference production of microcystin-RR (62-85%) and microcystin-LR (>98%) respectively. We detected the intracellular microcystins in lake water and microcystin-RR was a main variant (mostly>50%). In summary, McyBA1 subgroups were dominant within the population of toxic Microcystis and contributed the predominance of microcystin-RR in the lake. The differences of substrate preference in microcystin biosynthesis among groups were caused by neutral evolution and homologous recombination.
关键词[WOS]: MAXIMUM-LIKELIHOOD ; ENVIRONMENTAL-FACTORS ; ADENYLATION DOMAINS ; POSITIVE SELECTION ; CYANOBACTERIA ; AERUGINOSA ; DIVERSITY ; BLOOMS ; WATER ; BIOSYNTHESIS
语种: 英语
WOS记录号: WOS:000325142700002
ISSN号: 1758-2229
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19618
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Jiangxi Normal Univ, Sch Life Sci, Nanchang 330022, Peoples R China

Recommended Citation:
Jiang, Yongguang; Yu, Gongliang; Chai, Wenbo; Song, Gaofei; Li, Renhui.Congruence between mcy based genetic type and microcystin composition within the populations of toxic Microcystis in a plateau lake, China,ENVIRONMENTAL MICROBIOLOGY REPORTS,2013,5(5):637-647
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