IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Apoptotic responses of zebrafish (Danio rerio) after exposure with microcystin-LR under different ambient temperatures
Ji, Wei1,2; Liang, Hualei1; Zhou, Wenshan1; Zhang, Xuezhen1; Zhang, XZ (reprint author), Huazhong Agr Univ, Coll Fisheries, Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ, Wuhan 430070, Peoples R China.
2013-08-01
Source PublicationJOURNAL OF APPLIED TOXICOLOGY
ISSN0260-437X
Volume33Issue:8Pages:799-806
AbstractMicrocystins (MCs) can cause evident hepatic apoptosis. In vitro studies indicated that uptake of MC by isolated hepatocytes was dramatically reduced as ambient temperature dropped, and some studies presented a hypothesis that differences in core body temperatures in animals result in diverse uptake of MC, as well as different toxic effects. Thus far, however, few in vivo studies have been conducted to investigate the effects of temperature on MC-induced hepatocyte apoptosis in fish, a typical poikilotherm. In the present study, zebrafish were treated with MC-LR, an MC metabolite, at three water temperatures (12, 22 and 32 degrees C), and evident differences in apoptotic profiles were observed. Damage to liver ultrastructures revealed temperature-dependent early-stage patterns of apoptosis. Flow-cytometric analysis indicated that hepatocyte apoptotic rates varied with a temperature-dependent effect. The transcription levels of some apoptosis-related genes were determined using quantitative real-time polymerase chain reaction, and significantly elevated gene expressions of P53, Bcl-2, Bax and caspase-3 were found in the 12 and 32 degrees C groups. Results of the present study indicate that different ambient temperatures can lead to various toxic effects of MCs on hepatic apoptosis in fish. Copyright (c) 2012 John Wiley & Sons, Ltd.; Microcystins (MCs) can cause evident hepatic apoptosis. In vitro studies indicated that uptake of MC by isolated hepatocytes was dramatically reduced as ambient temperature dropped, and some studies presented a hypothesis that differences in core body temperatures in animals result in diverse uptake of MC, as well as different toxic effects. Thus far, however, few in vivo studies have been conducted to investigate the effects of temperature on MC-induced hepatocyte apoptosis in fish, a typical poikilotherm. In the present study, zebrafish were treated with MC-LR, an MC metabolite, at three water temperatures (12, 22 and 32 degrees C), and evident differences in apoptotic profiles were observed. Damage to liver ultrastructures revealed temperature-dependent early-stage patterns of apoptosis. Flow-cytometric analysis indicated that hepatocyte apoptotic rates varied with a temperature-dependent effect. The transcription levels of some apoptosis-related genes were determined using quantitative real-time polymerase chain reaction, and significantly elevated gene expressions of P53, Bcl-2, Bax and caspase-3 were found in the 12 and 32 degrees C groups. Results of the present study indicate that different ambient temperatures can lead to various toxic effects of MCs on hepatic apoptosis in fish. Copyright (c) 2012 John Wiley & Sons, Ltd.
SubtypeArticle
KeywordCyanobacterial Toxin Temperature Ultrastructural Changes Aapoptosis Real-time Pcr
Department[Ji, Wei ; Liang, Hualei ; Zhou, Wenshan ; Zhang, Xuezhen] Huazhong Agr Univ, Coll Fisheries, Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ, Wuhan 430070, Peoples R China ; [Ji, Wei] Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China
DOI10.1002/jat.2735
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Science Foundation for Distinguished Young Scholars of Hubei Province [2010CDA095]; Fundamental Research Funds for the Central Universities [2011PY003] ; National Science Foundation for Distinguished Young Scholars of Hubei Province [2010CDA095]; Fundamental Research Funds for the Central Universities [2011PY003] ; National Science Foundation for Distinguished Young Scholars of Hubei Province [2010CDA095]; Fundamental Research Funds for the Central Universities [2011PY003] ; National Science Foundation for Distinguished Young Scholars of Hubei Province [2010CDA095]; Fundamental Research Funds for the Central Universities [2011PY003]
Indexed BySCI
Language英语
WOS Research AreaToxicology
WOS SubjectToxicology
WOS IDWOS:000320778400010
WOS KeywordCARP CYPRINUS-CARPIO ; TROUT ONCORHYNCHUS-MYKISS ; PROGRAMMED CELL-DEATH ; CYCLIC PEPTIDE TOXIN ; BIOCHEMICAL-CHARACTERIZATION ; CYANOBACTERIAL TOXIN ; TUMOR-SUPPRESSOR ; RAT HEPATOCYTES ; IN-VITRO ; P53
Funding OrganizationNational Science Foundation for Distinguished Young Scholars of Hubei Province [2010CDA095]; Fundamental Research Funds for the Central Universities [2011PY003] ; National Science Foundation for Distinguished Young Scholars of Hubei Province [2010CDA095]; Fundamental Research Funds for the Central Universities [2011PY003] ; National Science Foundation for Distinguished Young Scholars of Hubei Province [2010CDA095]; Fundamental Research Funds for the Central Universities [2011PY003] ; National Science Foundation for Distinguished Young Scholars of Hubei Province [2010CDA095]; Fundamental Research Funds for the Central Universities [2011PY003]
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19617
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorZhang, XZ (reprint author), Huazhong Agr Univ, Coll Fisheries, Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ, Wuhan 430070, Peoples R China.
Affiliation1.Huazhong Agr Univ, Coll Fisheries, Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ, Wuhan 430070, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Ji, Wei,Liang, Hualei,Zhou, Wenshan,et al. Apoptotic responses of zebrafish (Danio rerio) after exposure with microcystin-LR under different ambient temperatures[J]. JOURNAL OF APPLIED TOXICOLOGY,2013,33(8):799-806.
APA Ji, Wei,Liang, Hualei,Zhou, Wenshan,Zhang, Xuezhen,&Zhang, XZ .(2013).Apoptotic responses of zebrafish (Danio rerio) after exposure with microcystin-LR under different ambient temperatures.JOURNAL OF APPLIED TOXICOLOGY,33(8),799-806.
MLA Ji, Wei,et al."Apoptotic responses of zebrafish (Danio rerio) after exposure with microcystin-LR under different ambient temperatures".JOURNAL OF APPLIED TOXICOLOGY 33.8(2013):799-806.
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