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题名: Apoptotic responses of zebrafish (Danio rerio) after exposure with microcystin-LR under different ambient temperatures
作者: Ji, Wei1, 2; Liang, Hualei1; Zhou, Wenshan1; Zhang, Xuezhen1
通讯作者: Zhang, XZ (reprint author), Huazhong Agr Univ, Coll Fisheries, Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ, Wuhan 430070, Peoples R China.
关键词: cyanobacterial toxin ; temperature ; ultrastructural changes ; aapoptosis ; real-time PCR
刊名: JOURNAL OF APPLIED TOXICOLOGY
发表日期: 2013-08-01
DOI: 10.1002/jat.2735
卷: 33, 期:8, 页:799-806
收录类别: SCI
文章类型: Article
部门归属: [Ji, Wei ; Liang, Hualei ; Zhou, Wenshan ; Zhang, Xuezhen] Huazhong Agr Univ, Coll Fisheries, Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ, Wuhan 430070, Peoples R China ; [Ji, Wei] Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Science Foundation for Distinguished Young Scholars of Hubei Province [2010CDA095]; Fundamental Research Funds for the Central Universities [2011PY003]
类目[WOS]: Toxicology
研究领域[WOS]: Toxicology
摘要: Microcystins (MCs) can cause evident hepatic apoptosis. In vitro studies indicated that uptake of MC by isolated hepatocytes was dramatically reduced as ambient temperature dropped, and some studies presented a hypothesis that differences in core body temperatures in animals result in diverse uptake of MC, as well as different toxic effects. Thus far, however, few in vivo studies have been conducted to investigate the effects of temperature on MC-induced hepatocyte apoptosis in fish, a typical poikilotherm. In the present study, zebrafish were treated with MC-LR, an MC metabolite, at three water temperatures (12, 22 and 32 degrees C), and evident differences in apoptotic profiles were observed. Damage to liver ultrastructures revealed temperature-dependent early-stage patterns of apoptosis. Flow-cytometric analysis indicated that hepatocyte apoptotic rates varied with a temperature-dependent effect. The transcription levels of some apoptosis-related genes were determined using quantitative real-time polymerase chain reaction, and significantly elevated gene expressions of P53, Bcl-2, Bax and caspase-3 were found in the 12 and 32 degrees C groups. Results of the present study indicate that different ambient temperatures can lead to various toxic effects of MCs on hepatic apoptosis in fish. Copyright (c) 2012 John Wiley & Sons, Ltd.
英文摘要: Microcystins (MCs) can cause evident hepatic apoptosis. In vitro studies indicated that uptake of MC by isolated hepatocytes was dramatically reduced as ambient temperature dropped, and some studies presented a hypothesis that differences in core body temperatures in animals result in diverse uptake of MC, as well as different toxic effects. Thus far, however, few in vivo studies have been conducted to investigate the effects of temperature on MC-induced hepatocyte apoptosis in fish, a typical poikilotherm. In the present study, zebrafish were treated with MC-LR, an MC metabolite, at three water temperatures (12, 22 and 32 degrees C), and evident differences in apoptotic profiles were observed. Damage to liver ultrastructures revealed temperature-dependent early-stage patterns of apoptosis. Flow-cytometric analysis indicated that hepatocyte apoptotic rates varied with a temperature-dependent effect. The transcription levels of some apoptosis-related genes were determined using quantitative real-time polymerase chain reaction, and significantly elevated gene expressions of P53, Bcl-2, Bax and caspase-3 were found in the 12 and 32 degrees C groups. Results of the present study indicate that different ambient temperatures can lead to various toxic effects of MCs on hepatic apoptosis in fish. Copyright (c) 2012 John Wiley & Sons, Ltd.
关键词[WOS]: CARP CYPRINUS-CARPIO ; TROUT ONCORHYNCHUS-MYKISS ; PROGRAMMED CELL-DEATH ; CYCLIC PEPTIDE TOXIN ; BIOCHEMICAL-CHARACTERIZATION ; CYANOBACTERIAL TOXIN ; TUMOR-SUPPRESSOR ; RAT HEPATOCYTES ; IN-VITRO ; P53
语种: 英语
WOS记录号: WOS:000320778400010
ISSN号: 0260-437X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19617
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Huazhong Agr Univ, Coll Fisheries, Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ, Wuhan 430070, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China

Recommended Citation:
Ji, Wei; Liang, Hualei; Zhou, Wenshan; Zhang, Xuezhen.Apoptotic responses of zebrafish (Danio rerio) after exposure with microcystin-LR under different ambient temperatures,JOURNAL OF APPLIED TOXICOLOGY,2013,33(8):799-806
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