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Eafs Control Erythroid Cell Fate by Regulating c-myb Expression through Wnt Signaling
Ma, Xufa1; Liu, Jing-Xia2; Liu, JX (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China.
2013-05-22
Source PublicationPLOS ONE
ISSN1932-6203
Volume8Issue:5Pages:e64576
AbstractELL associated factor 1 and ELL associated factor 2 (EAF1/2 factors) are reported to play important roles in tumor suppression and embryogenesis. Our previous studies showed that eaf factors mediated effective convergence and extension (C&E) movements and modulated mesoderm and neural patterning by regulating both non-canonical and canonical Wnt signaling in the early embryonic process. In this study, through knockdown of both eaf1 and eaf2 in embryos, we found that differentiation of primary erythroid cells was blocked, but hematopoietic precursor cells maintained in eafs morphants. Co-injection of c-myb-MO rescued the erythroid differentiation in eafs morphants, as indicated by the restored expression of the erythroid-specific gene, beta e3 globin. In addition, low dosage of c-myb effectively blocked the beta e3 globin expression in embryos, and did not affect the expression of markers of hematopoietic progenitor cells and other mesoderm, which was similar to the phenotypes we observed in eafs morphants. We also revealed that knockdown Wnt signaling by transiently inducing dn-Tcf in embryos at the bud stage down-regulated the increased c-myb to normal level and also restored beta e3 globin expression in eafs morphants. Our evidence points to a novel role for eaf factors in controlling erythroid cell fate by regulating c-Myb expression through canonic Wnt signaling.; ELL associated factor 1 and ELL associated factor 2 (EAF1/2 factors) are reported to play important roles in tumor suppression and embryogenesis. Our previous studies showed that eaf factors mediated effective convergence and extension (C&E) movements and modulated mesoderm and neural patterning by regulating both non-canonical and canonical Wnt signaling in the early embryonic process. In this study, through knockdown of both eaf1 and eaf2 in embryos, we found that differentiation of primary erythroid cells was blocked, but hematopoietic precursor cells maintained in eafs morphants. Co-injection of c-myb-MO rescued the erythroid differentiation in eafs morphants, as indicated by the restored expression of the erythroid-specific gene, beta e3 globin. In addition, low dosage of c-myb effectively blocked the beta e3 globin expression in embryos, and did not affect the expression of markers of hematopoietic progenitor cells and other mesoderm, which was similar to the phenotypes we observed in eafs morphants. We also revealed that knockdown Wnt signaling by transiently inducing dn-Tcf in embryos at the bud stage down-regulated the increased c-myb to normal level and also restored beta e3 globin expression in eafs morphants. Our evidence points to a novel role for eaf factors in controlling erythroid cell fate by regulating c-Myb expression through canonic Wnt signaling.
SubtypeArticle
KeywordHematopoietic Stem-cells Transcription Factor Self-renewal Zebrafish Embryos Dna-binding Differentiation Gata-1 Specification Activation Gene
Department[Ma, Xufa] Huazhong Agr Univ, Coll Fisheries, Wuhan, Peoples R China ; [Liu, Jing-Xia] Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
DOI10.1371/journal.pone.0064576
WOS HeadingsScience & Technology
Funding OrganizationChinese Academy of Sciences [KSCX2-EW-Q-12] ; Chinese Academy of Sciences [KSCX2-EW-Q-12] ; Chinese Academy of Sciences [KSCX2-EW-Q-12] ; Chinese Academy of Sciences [KSCX2-EW-Q-12]
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000320362700156
WOS KeywordHEMATOPOIETIC STEM-CELLS ; TRANSCRIPTION FACTOR ; SELF-RENEWAL ; ZEBRAFISH EMBRYOS ; DNA-BINDING ; DIFFERENTIATION ; GATA-1 ; SPECIFICATION ; ACTIVATION ; GENE
Funding OrganizationChinese Academy of Sciences [KSCX2-EW-Q-12] ; Chinese Academy of Sciences [KSCX2-EW-Q-12] ; Chinese Academy of Sciences [KSCX2-EW-Q-12] ; Chinese Academy of Sciences [KSCX2-EW-Q-12]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19615
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorLiu, JX (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China.
Affiliation1.Huazhong Agr Univ, Coll Fisheries, Wuhan, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Ma, Xufa,Liu, Jing-Xia,Liu, JX . Eafs Control Erythroid Cell Fate by Regulating c-myb Expression through Wnt Signaling[J]. PLOS ONE,2013,8(5):e64576.
APA Ma, Xufa,Liu, Jing-Xia,&Liu, JX .(2013).Eafs Control Erythroid Cell Fate by Regulating c-myb Expression through Wnt Signaling.PLOS ONE,8(5),e64576.
MLA Ma, Xufa,et al."Eafs Control Erythroid Cell Fate by Regulating c-myb Expression through Wnt Signaling".PLOS ONE 8.5(2013):e64576.
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