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题名: Eafs Control Erythroid Cell Fate by Regulating c-myb Expression through Wnt Signaling
作者: Ma, Xufa1; Liu, Jing-Xia2
通讯作者: Liu, JX (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China.
关键词: HEMATOPOIETIC STEM-CELLS ; TRANSCRIPTION FACTOR ; SELF-RENEWAL ; ZEBRAFISH EMBRYOS ; DNA-BINDING ; DIFFERENTIATION ; GATA-1 ; SPECIFICATION ; ACTIVATION ; GENE
刊名: PLOS ONE
发表日期: 2013-05-22
DOI: 10.1371/journal.pone.0064576
卷: 8, 期:5, 页:e64576
收录类别: SCI
文章类型: Article
部门归属: [Ma, Xufa] Huazhong Agr Univ, Coll Fisheries, Wuhan, Peoples R China ; [Liu, Jing-Xia] Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
WOS标题词: Science & Technology
资助者: Chinese Academy of Sciences [KSCX2-EW-Q-12]
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
摘要: ELL associated factor 1 and ELL associated factor 2 (EAF1/2 factors) are reported to play important roles in tumor suppression and embryogenesis. Our previous studies showed that eaf factors mediated effective convergence and extension (C&E) movements and modulated mesoderm and neural patterning by regulating both non-canonical and canonical Wnt signaling in the early embryonic process. In this study, through knockdown of both eaf1 and eaf2 in embryos, we found that differentiation of primary erythroid cells was blocked, but hematopoietic precursor cells maintained in eafs morphants. Co-injection of c-myb-MO rescued the erythroid differentiation in eafs morphants, as indicated by the restored expression of the erythroid-specific gene, beta e3 globin. In addition, low dosage of c-myb effectively blocked the beta e3 globin expression in embryos, and did not affect the expression of markers of hematopoietic progenitor cells and other mesoderm, which was similar to the phenotypes we observed in eafs morphants. We also revealed that knockdown Wnt signaling by transiently inducing dn-Tcf in embryos at the bud stage down-regulated the increased c-myb to normal level and also restored beta e3 globin expression in eafs morphants. Our evidence points to a novel role for eaf factors in controlling erythroid cell fate by regulating c-Myb expression through canonic Wnt signaling.
英文摘要: ELL associated factor 1 and ELL associated factor 2 (EAF1/2 factors) are reported to play important roles in tumor suppression and embryogenesis. Our previous studies showed that eaf factors mediated effective convergence and extension (C&E) movements and modulated mesoderm and neural patterning by regulating both non-canonical and canonical Wnt signaling in the early embryonic process. In this study, through knockdown of both eaf1 and eaf2 in embryos, we found that differentiation of primary erythroid cells was blocked, but hematopoietic precursor cells maintained in eafs morphants. Co-injection of c-myb-MO rescued the erythroid differentiation in eafs morphants, as indicated by the restored expression of the erythroid-specific gene, beta e3 globin. In addition, low dosage of c-myb effectively blocked the beta e3 globin expression in embryos, and did not affect the expression of markers of hematopoietic progenitor cells and other mesoderm, which was similar to the phenotypes we observed in eafs morphants. We also revealed that knockdown Wnt signaling by transiently inducing dn-Tcf in embryos at the bud stage down-regulated the increased c-myb to normal level and also restored beta e3 globin expression in eafs morphants. Our evidence points to a novel role for eaf factors in controlling erythroid cell fate by regulating c-Myb expression through canonic Wnt signaling.
关键词[WOS]: HEMATOPOIETIC STEM-CELLS ; TRANSCRIPTION FACTOR ; SELF-RENEWAL ; ZEBRAFISH EMBRYOS ; DNA-BINDING ; DIFFERENTIATION ; GATA-1 ; SPECIFICATION ; ACTIVATION ; GENE
语种: 英语
WOS记录号: WOS:000320362700156
ISSN号: 1932-6203
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19615
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Huazhong Agr Univ, Coll Fisheries, Wuhan, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China

Recommended Citation:
Ma, Xufa; Liu, Jing-Xia.Eafs Control Erythroid Cell Fate by Regulating c-myb Expression through Wnt Signaling,PLOS ONE,2013,8(5):e64576
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