| Sulforaphane inhibits CYP1A1 activity and promotes genotoxicity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in vitro | |
| Yang, Fangxing1; Zhuang, Shulin1; Zhang, Chao2; Dai, Heping2; Liu, Weiping1; Yang, FX (reprint author), Zhejiang Univ, Coll Environm & Resource Sci, 388 Yuhangtang Rd, Hangzhou 310058, Peoples R China. | |
| 2013-06-15 | |
| Source Publication | TOXICOLOGY AND APPLIED PHARMACOLOGY
 |
| ISSN | 0041-008X |
| Volume | 269Issue:3Pages:226-232 |
| Abstract | Increasing environmental pollution by carcinogens such as some of persistent organic pollutants (POPs) has prompted growing interest in searching for chemopreventive compounds which are readily obtainable. Sulforaphane (SFN) is isolated from cruciferous vegetables and has the potentials to reduce carcinogenesis through various pathways. In this study, we studied the effects of SFN on CYP1A1 activity and genotoxicity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The results showed that SFN inhibited TCDD-induced CYP1A1 activity in H4IIE cells by directly inhibiting CYP1A1 activity, probably through binding to aryl hydrocarbon receptor and/or CYP1A1 revealed by molecular docking. However, SFN promoted TCDD-induced DNA damage in yeast cells and reduced the viability of initiated yeast cells. Besides, it is surprising that SFN also failed to reduce genotoxicity induced by other genotoxic reagents which possess different mechanisms to lead to DNA damage. Currently, it is difficult to predict whether SFN has the potentials to reduce the risk of TCDD based on the conflicting observations in the study. Therefore, further studies should be urgent to reveal the function and mechanism of SFN in the stress of such POPs on human health. (C) 2013 Elsevier Inc. All rights reserved.; Increasing environmental pollution by carcinogens such as some of persistent organic pollutants (POPs) has prompted growing interest in searching for chemopreventive compounds which are readily obtainable. Sulforaphane (SFN) is isolated from cruciferous vegetables and has the potentials to reduce carcinogenesis through various pathways. In this study, we studied the effects of SFN on CYP1A1 activity and genotoxicity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The results showed that SFN inhibited TCDD-induced CYP1A1 activity in H4IIE cells by directly inhibiting CYP1A1 activity, probably through binding to aryl hydrocarbon receptor and/or CYP1A1 revealed by molecular docking. However, SFN promoted TCDD-induced DNA damage in yeast cells and reduced the viability of initiated yeast cells. Besides, it is surprising that SFN also failed to reduce genotoxicity induced by other genotoxic reagents which possess different mechanisms to lead to DNA damage. Currently, it is difficult to predict whether SFN has the potentials to reduce the risk of TCDD based on the conflicting observations in the study. Therefore, further studies should be urgent to reveal the function and mechanism of SFN in the stress of such POPs on human health. (C) 2013 Elsevier Inc. All rights reserved. |
| Subtype | Article |
| Keyword | Sulforaphane 2,3,7,8-tetrachlorodibenzo-p-dioxin Cyp1a1 Activity H4iie Cell Genotoxicity Yeast |
| Department | [Yang, Fangxing ; Zhuang, Shulin ; Liu, Weiping] Zhejiang Univ, Coll Environm & Resource Sci, MOE Key Lab Environm Remediat & Ecosyst Hlth, Hangzhou 310058, Peoples R China ; [Zhang, Chao ; Dai, Heping] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| DOI | 10.1016/j.taap.2013.03.024 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | National Nature Science Foundation of China [20890113, 21037004, 21277122]; Zhejiang Provincial Natural Science Foundation of China [Y13B070003]; 863 Research Project [2009AA06Z407]; FEBL [2011FBZ10] ; National Nature Science Foundation of China [20890113, 21037004, 21277122]; Zhejiang Provincial Natural Science Foundation of China [Y13B070003]; 863 Research Project [2009AA06Z407]; FEBL [2011FBZ10] ; National Nature Science Foundation of China [20890113, 21037004, 21277122]; Zhejiang Provincial Natural Science Foundation of China [Y13B070003]; 863 Research Project [2009AA06Z407]; FEBL [2011FBZ10] ; National Nature Science Foundation of China [20890113, 21037004, 21277122]; Zhejiang Provincial Natural Science Foundation of China [Y13B070003]; 863 Research Project [2009AA06Z407]; FEBL [2011FBZ10] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Pharmacology & Pharmacy ; Toxicology |
| WOS Subject | Pharmacology & Pharmacy ; Toxicology |
| WOS ID | WOS:000319373100003 |
| WOS Keyword | ARYL-HYDROCARBON RECEPTOR ; SACCHAROMYCES-CEREVISIAE ; POLYCHLORINATED BIPHENYL ; TESTING SYSTEM ; CANCER-RISK ; CELLS ; EXPRESSION ; YEAST ; TCDD ; ETHOXYRESORUFIN |
| Funding Organization | National Nature Science Foundation of China [20890113, 21037004, 21277122]; Zhejiang Provincial Natural Science Foundation of China [Y13B070003]; 863 Research Project [2009AA06Z407]; FEBL [2011FBZ10] ; National Nature Science Foundation of China [20890113, 21037004, 21277122]; Zhejiang Provincial Natural Science Foundation of China [Y13B070003]; 863 Research Project [2009AA06Z407]; FEBL [2011FBZ10] ; National Nature Science Foundation of China [20890113, 21037004, 21277122]; Zhejiang Provincial Natural Science Foundation of China [Y13B070003]; 863 Research Project [2009AA06Z407]; FEBL [2011FBZ10] ; National Nature Science Foundation of China [20890113, 21037004, 21277122]; Zhejiang Provincial Natural Science Foundation of China [Y13B070003]; 863 Research Project [2009AA06Z407]; FEBL [2011FBZ10] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/19614 |
| Collection | 水生生物分子与细胞生物学研究中心_期刊论文 |
| Corresponding Author | Yang, FX (reprint author), Zhejiang Univ, Coll Environm & Resource Sci, 388 Yuhangtang Rd, Hangzhou 310058, Peoples R China. |
| Affiliation | 1.Zhejiang Univ, Coll Environm & Resource Sci, MOE Key Lab Environm Remediat & Ecosyst Hlth, Hangzhou 310058, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Yang, Fangxing,Zhuang, Shulin,Zhang, Chao,et al. Sulforaphane inhibits CYP1A1 activity and promotes genotoxicity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in vitro[J]. TOXICOLOGY AND APPLIED PHARMACOLOGY,2013,269(3):226-232. |
| APA | Yang, Fangxing,Zhuang, Shulin,Zhang, Chao,Dai, Heping,Liu, Weiping,&Yang, FX .(2013).Sulforaphane inhibits CYP1A1 activity and promotes genotoxicity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in vitro.TOXICOLOGY AND APPLIED PHARMACOLOGY,269(3),226-232. |
| MLA | Yang, Fangxing,et al."Sulforaphane inhibits CYP1A1 activity and promotes genotoxicity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in vitro".TOXICOLOGY AND APPLIED PHARMACOLOGY 269.3(2013):226-232. |
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