IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Sulforaphane inhibits CYP1A1 activity and promotes genotoxicity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in vitro
Yang, Fangxing1; Zhuang, Shulin1; Zhang, Chao2; Dai, Heping2; Liu, Weiping1; Yang, FX (reprint author), Zhejiang Univ, Coll Environm & Resource Sci, 388 Yuhangtang Rd, Hangzhou 310058, Peoples R China.
2013-06-15
Source PublicationTOXICOLOGY AND APPLIED PHARMACOLOGY
ISSN0041-008X
Volume269Issue:3Pages:226-232
AbstractIncreasing environmental pollution by carcinogens such as some of persistent organic pollutants (POPs) has prompted growing interest in searching for chemopreventive compounds which are readily obtainable. Sulforaphane (SFN) is isolated from cruciferous vegetables and has the potentials to reduce carcinogenesis through various pathways. In this study, we studied the effects of SFN on CYP1A1 activity and genotoxicity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The results showed that SFN inhibited TCDD-induced CYP1A1 activity in H4IIE cells by directly inhibiting CYP1A1 activity, probably through binding to aryl hydrocarbon receptor and/or CYP1A1 revealed by molecular docking. However, SFN promoted TCDD-induced DNA damage in yeast cells and reduced the viability of initiated yeast cells. Besides, it is surprising that SFN also failed to reduce genotoxicity induced by other genotoxic reagents which possess different mechanisms to lead to DNA damage. Currently, it is difficult to predict whether SFN has the potentials to reduce the risk of TCDD based on the conflicting observations in the study. Therefore, further studies should be urgent to reveal the function and mechanism of SFN in the stress of such POPs on human health. (C) 2013 Elsevier Inc. All rights reserved.; Increasing environmental pollution by carcinogens such as some of persistent organic pollutants (POPs) has prompted growing interest in searching for chemopreventive compounds which are readily obtainable. Sulforaphane (SFN) is isolated from cruciferous vegetables and has the potentials to reduce carcinogenesis through various pathways. In this study, we studied the effects of SFN on CYP1A1 activity and genotoxicity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The results showed that SFN inhibited TCDD-induced CYP1A1 activity in H4IIE cells by directly inhibiting CYP1A1 activity, probably through binding to aryl hydrocarbon receptor and/or CYP1A1 revealed by molecular docking. However, SFN promoted TCDD-induced DNA damage in yeast cells and reduced the viability of initiated yeast cells. Besides, it is surprising that SFN also failed to reduce genotoxicity induced by other genotoxic reagents which possess different mechanisms to lead to DNA damage. Currently, it is difficult to predict whether SFN has the potentials to reduce the risk of TCDD based on the conflicting observations in the study. Therefore, further studies should be urgent to reveal the function and mechanism of SFN in the stress of such POPs on human health. (C) 2013 Elsevier Inc. All rights reserved.
SubtypeArticle
KeywordSulforaphane 2,3,7,8-tetrachlorodibenzo-p-dioxin Cyp1a1 Activity H4iie Cell Genotoxicity Yeast
Department[Yang, Fangxing ; Zhuang, Shulin ; Liu, Weiping] Zhejiang Univ, Coll Environm & Resource Sci, MOE Key Lab Environm Remediat & Ecosyst Hlth, Hangzhou 310058, Peoples R China ; [Zhang, Chao ; Dai, Heping] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
DOI10.1016/j.taap.2013.03.024
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Nature Science Foundation of China [20890113, 21037004, 21277122]; Zhejiang Provincial Natural Science Foundation of China [Y13B070003]; 863 Research Project [2009AA06Z407]; FEBL [2011FBZ10] ; National Nature Science Foundation of China [20890113, 21037004, 21277122]; Zhejiang Provincial Natural Science Foundation of China [Y13B070003]; 863 Research Project [2009AA06Z407]; FEBL [2011FBZ10] ; National Nature Science Foundation of China [20890113, 21037004, 21277122]; Zhejiang Provincial Natural Science Foundation of China [Y13B070003]; 863 Research Project [2009AA06Z407]; FEBL [2011FBZ10] ; National Nature Science Foundation of China [20890113, 21037004, 21277122]; Zhejiang Provincial Natural Science Foundation of China [Y13B070003]; 863 Research Project [2009AA06Z407]; FEBL [2011FBZ10]
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy ; Toxicology
WOS SubjectPharmacology & Pharmacy ; Toxicology
WOS IDWOS:000319373100003
WOS KeywordARYL-HYDROCARBON RECEPTOR ; SACCHAROMYCES-CEREVISIAE ; POLYCHLORINATED BIPHENYL ; TESTING SYSTEM ; CANCER-RISK ; CELLS ; EXPRESSION ; YEAST ; TCDD ; ETHOXYRESORUFIN
Funding OrganizationNational Nature Science Foundation of China [20890113, 21037004, 21277122]; Zhejiang Provincial Natural Science Foundation of China [Y13B070003]; 863 Research Project [2009AA06Z407]; FEBL [2011FBZ10] ; National Nature Science Foundation of China [20890113, 21037004, 21277122]; Zhejiang Provincial Natural Science Foundation of China [Y13B070003]; 863 Research Project [2009AA06Z407]; FEBL [2011FBZ10] ; National Nature Science Foundation of China [20890113, 21037004, 21277122]; Zhejiang Provincial Natural Science Foundation of China [Y13B070003]; 863 Research Project [2009AA06Z407]; FEBL [2011FBZ10] ; National Nature Science Foundation of China [20890113, 21037004, 21277122]; Zhejiang Provincial Natural Science Foundation of China [Y13B070003]; 863 Research Project [2009AA06Z407]; FEBL [2011FBZ10]
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19614
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorYang, FX (reprint author), Zhejiang Univ, Coll Environm & Resource Sci, 388 Yuhangtang Rd, Hangzhou 310058, Peoples R China.
Affiliation1.Zhejiang Univ, Coll Environm & Resource Sci, MOE Key Lab Environm Remediat & Ecosyst Hlth, Hangzhou 310058, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Yang, Fangxing,Zhuang, Shulin,Zhang, Chao,et al. Sulforaphane inhibits CYP1A1 activity and promotes genotoxicity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in vitro[J]. TOXICOLOGY AND APPLIED PHARMACOLOGY,2013,269(3):226-232.
APA Yang, Fangxing,Zhuang, Shulin,Zhang, Chao,Dai, Heping,Liu, Weiping,&Yang, FX .(2013).Sulforaphane inhibits CYP1A1 activity and promotes genotoxicity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in vitro.TOXICOLOGY AND APPLIED PHARMACOLOGY,269(3),226-232.
MLA Yang, Fangxing,et al."Sulforaphane inhibits CYP1A1 activity and promotes genotoxicity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in vitro".TOXICOLOGY AND APPLIED PHARMACOLOGY 269.3(2013):226-232.
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