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题名: Sulforaphane inhibits CYP1A1 activity and promotes genotoxicity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in vitro
作者: Yang, Fangxing1; Zhuang, Shulin1; Zhang, Chao2; Dai, Heping2; Liu, Weiping1
通讯作者: Yang, FX (reprint author), Zhejiang Univ, Coll Environm & Resource Sci, 388 Yuhangtang Rd, Hangzhou 310058, Peoples R China.
关键词: Sulforaphane ; 2,3,7,8-Tetrachlorodibenzo-p-dioxin ; CYP1A1 activity ; H4IIE cell ; Genotoxicity ; Yeast
刊名: TOXICOLOGY AND APPLIED PHARMACOLOGY
发表日期: 2013-06-15
DOI: 10.1016/j.taap.2013.03.024
卷: 269, 期:3, 页:226-232
收录类别: SCI
文章类型: Article
部门归属: [Yang, Fangxing ; Zhuang, Shulin ; Liu, Weiping] Zhejiang Univ, Coll Environm & Resource Sci, MOE Key Lab Environm Remediat & Ecosyst Hlth, Hangzhou 310058, Peoples R China ; [Zhang, Chao ; Dai, Heping] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Nature Science Foundation of China [20890113, 21037004, 21277122]; Zhejiang Provincial Natural Science Foundation of China [Y13B070003]; 863 Research Project [2009AA06Z407]; FEBL [2011FBZ10]
类目[WOS]: Pharmacology & Pharmacy ; Toxicology
研究领域[WOS]: Pharmacology & Pharmacy ; Toxicology
摘要: Increasing environmental pollution by carcinogens such as some of persistent organic pollutants (POPs) has prompted growing interest in searching for chemopreventive compounds which are readily obtainable. Sulforaphane (SFN) is isolated from cruciferous vegetables and has the potentials to reduce carcinogenesis through various pathways. In this study, we studied the effects of SFN on CYP1A1 activity and genotoxicity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The results showed that SFN inhibited TCDD-induced CYP1A1 activity in H4IIE cells by directly inhibiting CYP1A1 activity, probably through binding to aryl hydrocarbon receptor and/or CYP1A1 revealed by molecular docking. However, SFN promoted TCDD-induced DNA damage in yeast cells and reduced the viability of initiated yeast cells. Besides, it is surprising that SFN also failed to reduce genotoxicity induced by other genotoxic reagents which possess different mechanisms to lead to DNA damage. Currently, it is difficult to predict whether SFN has the potentials to reduce the risk of TCDD based on the conflicting observations in the study. Therefore, further studies should be urgent to reveal the function and mechanism of SFN in the stress of such POPs on human health. (C) 2013 Elsevier Inc. All rights reserved.
英文摘要: Increasing environmental pollution by carcinogens such as some of persistent organic pollutants (POPs) has prompted growing interest in searching for chemopreventive compounds which are readily obtainable. Sulforaphane (SFN) is isolated from cruciferous vegetables and has the potentials to reduce carcinogenesis through various pathways. In this study, we studied the effects of SFN on CYP1A1 activity and genotoxicity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The results showed that SFN inhibited TCDD-induced CYP1A1 activity in H4IIE cells by directly inhibiting CYP1A1 activity, probably through binding to aryl hydrocarbon receptor and/or CYP1A1 revealed by molecular docking. However, SFN promoted TCDD-induced DNA damage in yeast cells and reduced the viability of initiated yeast cells. Besides, it is surprising that SFN also failed to reduce genotoxicity induced by other genotoxic reagents which possess different mechanisms to lead to DNA damage. Currently, it is difficult to predict whether SFN has the potentials to reduce the risk of TCDD based on the conflicting observations in the study. Therefore, further studies should be urgent to reveal the function and mechanism of SFN in the stress of such POPs on human health. (C) 2013 Elsevier Inc. All rights reserved.
关键词[WOS]: ARYL-HYDROCARBON RECEPTOR ; SACCHAROMYCES-CEREVISIAE ; POLYCHLORINATED BIPHENYL ; TESTING SYSTEM ; CANCER-RISK ; CELLS ; EXPRESSION ; YEAST ; TCDD ; ETHOXYRESORUFIN
语种: 英语
WOS记录号: WOS:000319373100003
ISSN号: 0041-008X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19614
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Zhejiang Univ, Coll Environm & Resource Sci, MOE Key Lab Environm Remediat & Ecosyst Hlth, Hangzhou 310058, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

Recommended Citation:
Yang, Fangxing; Zhuang, Shulin; Zhang, Chao; Dai, Heping; Liu, Weiping.Sulforaphane inhibits CYP1A1 activity and promotes genotoxicity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in vitro,TOXICOLOGY AND APPLIED PHARMACOLOGY,2013,269(3):226-232
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