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Predominance of ammonia-oxidizing archaea and nirK-gene-bearing denitrifiers among ammonia-oxidizing and denitrifying populations in sediments of a large urban eutrophic lake (Lake Donghu)
Hou, Jie1,2; Cao, Xiuyun1; Song, Chunlei1; Zhou, Yiyong1; Zhou, YY (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, 7 Donghu South Rd, Wuhan, Hubei, Peoples R China.
2013-07-01
Source PublicationCANADIAN JOURNAL OF MICROBIOLOGY
ISSN0008-4166
Volume59Issue:7Pages:456-464
AbstractThe coupled nitrification-denitrification process plays a pivotal role in cycling and removal of nitrogen in aquatic ecosystems. In the present study, the communities of ammonia oxidizers and denitrifiers in the sediments of 2 basins (Guozhenghu Basin and Tuanhu Basin) of a large urban eutrophic lake (Lake Donghu) were determined using the ammonia monooxygenase subunit A (amoA) gene and the nitrite reductase gene. At all sites of this study, the archaeal amoA gene predominated over the bacterial amoA gene, whereas the functional gene for denitrification nirK gene far outnumbered the nirS gene. Spatially, compared with the Tuanhu Basin, the Guozhenghu Basin showed a significantly greater abundance of the archaeal amoA gene but less abundance of the nirK and nirS genes, while there was no significant difference of bacterial amoA gene copy numbers between the 2 basins. Unlike the archaeal amoA gene, the nirK gene showed a significant difference in community structure between the 2 basins. Archaeal amoA diversity was limited to the water-sediment cluster of Crenarchaeota, in sharp contrast with nirK for which 22 distinct operational taxonomic units were found. Accumulation of organic substances were found to be positively related to nirK and nirS gene copy numbers but negatively related to archaeal amoA gene copy numbers, whereas the abundance of the bacterial amoA gene was related to ammonia concentration.; The coupled nitrification-denitrification process plays a pivotal role in cycling and removal of nitrogen in aquatic ecosystems. In the present study, the communities of ammonia oxidizers and denitrifiers in the sediments of 2 basins (Guozhenghu Basin and Tuanhu Basin) of a large urban eutrophic lake (Lake Donghu) were determined using the ammonia monooxygenase subunit A (amoA) gene and the nitrite reductase gene. At all sites of this study, the archaeal amoA gene predominated over the bacterial amoA gene, whereas the functional gene for denitrification nirK gene far outnumbered the nirS gene. Spatially, compared with the Tuanhu Basin, the Guozhenghu Basin showed a significantly greater abundance of the archaeal amoA gene but less abundance of the nirK and nirS genes, while there was no significant difference of bacterial amoA gene copy numbers between the 2 basins. Unlike the archaeal amoA gene, the nirK gene showed a significant difference in community structure between the 2 basins. Archaeal amoA diversity was limited to the water-sediment cluster of Crenarchaeota, in sharp contrast with nirK for which 22 distinct operational taxonomic units were found. Accumulation of organic substances were found to be positively related to nirK and nirS gene copy numbers but negatively related to archaeal amoA gene copy numbers, whereas the abundance of the bacterial amoA gene was related to ammonia concentration.
SubtypeArticle
KeywordUrban Eutrophic Lake Amoa Nirk Nirs Qpcr
Department[Hou, Jie ; Cao, Xiuyun ; Song, Chunlei ; Zhou, Yiyong] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Hubei, Peoples R China ; [Hou, Jie] Univ Chinese Acad Sci, Beijing 100039, Peoples R China
DOI10.1139/cjm-2013-0083
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Science Foundation of China [41230748, 41073066, 41173081]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ15] ; National Science Foundation of China [41230748, 41073066, 41173081]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ15] ; National Science Foundation of China [41230748, 41073066, 41173081]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ15] ; National Science Foundation of China [41230748, 41073066, 41173081]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ15]
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology ; Microbiology
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology ; Microbiology
WOS IDWOS:000321330800003
WOS KeywordGRADIENT GEL-ELECTROPHORESIS ; FRANCISCO BAY ESTUARY ; COMMUNITY COMPOSITION ; ABUNDANCE ; BACTERIA ; DIVERSITY ; CHINA ; REDUCTASE ; SOIL ; HETEROGENEITY
Funding OrganizationNational Science Foundation of China [41230748, 41073066, 41173081]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ15] ; National Science Foundation of China [41230748, 41073066, 41173081]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ15] ; National Science Foundation of China [41230748, 41073066, 41173081]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ15] ; National Science Foundation of China [41230748, 41073066, 41173081]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ15]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19600
Collection水环境工程研究中心_期刊论文
Corresponding AuthorZhou, YY (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, 7 Donghu South Rd, Wuhan, Hubei, Peoples R China.
Affiliation1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Hou, Jie,Cao, Xiuyun,Song, Chunlei,et al. Predominance of ammonia-oxidizing archaea and nirK-gene-bearing denitrifiers among ammonia-oxidizing and denitrifying populations in sediments of a large urban eutrophic lake (Lake Donghu)[J]. CANADIAN JOURNAL OF MICROBIOLOGY,2013,59(7):456-464.
APA Hou, Jie,Cao, Xiuyun,Song, Chunlei,Zhou, Yiyong,&Zhou, YY .(2013).Predominance of ammonia-oxidizing archaea and nirK-gene-bearing denitrifiers among ammonia-oxidizing and denitrifying populations in sediments of a large urban eutrophic lake (Lake Donghu).CANADIAN JOURNAL OF MICROBIOLOGY,59(7),456-464.
MLA Hou, Jie,et al."Predominance of ammonia-oxidizing archaea and nirK-gene-bearing denitrifiers among ammonia-oxidizing and denitrifying populations in sediments of a large urban eutrophic lake (Lake Donghu)".CANADIAN JOURNAL OF MICROBIOLOGY 59.7(2013):456-464.
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