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Acute Toxicity of Heavy Metals to Tetrahymena in an In Vitro Experiment and Envelope Damage Study
Zhang, Tian1; Li, Xi1; Lu, Yang1; Wu, Chunyun1; Fang, Tingting1; Liu, Peng1; Zhang, Chaocan2; Liang, Wei3; Li, X (reprint author), Wuhan Univ Technol, Sch Sci, Dept Chem, Wuhan 430070, Peoples R China.
2013-07-01
Source PublicationBULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
ISSN0007-4861
Volume91Issue:1Pages:62-68
AbstractThe toxicity of Cr3+, Cu2+ and Cd2+ to Tetrahymena growth metabolism was studied by microcalorimetry at 28A degrees C, and the growth constant (k), peak time (T) and generation times (T (G)) were calculated. The metal ion concentrations that resulted in 50 % inhibition (IC (50)) of population growth were obtained through the dynamic parameters. The results indicated that the order of toxicity was Cd2+ > Cr3+ > Cu2+. Inductively coupled plasma-atomic emission spectrometry results suggested that the metal ions affected the permeability of the cell membrane. Observations of the Cd-exposed organisms by scanning electron microscopy revealed damage to the cell membrane in the form of an altered surface appearance. The cells suffered serious damage after sufficient acting time. Attenuated total reflection Fourier transform infrared spectra revealed that amide groups and PO2 (-) of the phospholipid phospho-diester, both located in the hydrophobic end of the outer layer of the cell membrane, were most readily affected.; The toxicity of Cr3+, Cu2+ and Cd2+ to Tetrahymena growth metabolism was studied by microcalorimetry at 28A degrees C, and the growth constant (k), peak time (T) and generation times (T (G)) were calculated. The metal ion concentrations that resulted in 50 % inhibition (IC (50)) of population growth were obtained through the dynamic parameters. The results indicated that the order of toxicity was Cd2+ > Cr3+ > Cu2+. Inductively coupled plasma-atomic emission spectrometry results suggested that the metal ions affected the permeability of the cell membrane. Observations of the Cd-exposed organisms by scanning electron microscopy revealed damage to the cell membrane in the form of an altered surface appearance. The cells suffered serious damage after sufficient acting time. Attenuated total reflection Fourier transform infrared spectra revealed that amide groups and PO2 (-) of the phospholipid phospho-diester, both located in the hydrophobic end of the outer layer of the cell membrane, were most readily affected.
SubtypeArticle
KeywordMetal Tetrahymena Microcalorimetry Toxicity Membrane
Department[Zhang, Tian ; Li, Xi ; Lu, Yang ; Wu, Chunyun ; Fang, Tingting ; Liu, Peng] Wuhan Univ Technol, Sch Sci, Dept Chem, Wuhan 430070, Peoples R China ; [Zhang, Chaocan] Wuhan Univ Technol, Sch Mat Sci & Engn, Wuhan 430070, Peoples R China ; [Liang, Wei] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
DOI10.1007/s00128-013-1004-9
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationMajor Science and Technology Program for Water Pollution Control and Treatment [2009ZX07106-003] ; Major Science and Technology Program for Water Pollution Control and Treatment [2009ZX07106-003] ; Major Science and Technology Program for Water Pollution Control and Treatment [2009ZX07106-003] ; Major Science and Technology Program for Water Pollution Control and Treatment [2009ZX07106-003]
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Toxicology
WOS SubjectEnvironmental Sciences ; Toxicology
WOS IDWOS:000320881100011
WOS KeywordACCUMULATION ; SOILS
Funding OrganizationMajor Science and Technology Program for Water Pollution Control and Treatment [2009ZX07106-003] ; Major Science and Technology Program for Water Pollution Control and Treatment [2009ZX07106-003] ; Major Science and Technology Program for Water Pollution Control and Treatment [2009ZX07106-003] ; Major Science and Technology Program for Water Pollution Control and Treatment [2009ZX07106-003]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19598
Collection水环境工程研究中心_期刊论文
Corresponding AuthorLi, X (reprint author), Wuhan Univ Technol, Sch Sci, Dept Chem, Wuhan 430070, Peoples R China.
Affiliation1.Wuhan Univ Technol, Sch Sci, Dept Chem, Wuhan 430070, Peoples R China
2.Wuhan Univ Technol, Sch Mat Sci & Engn, Wuhan 430070, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Tian,Li, Xi,Lu, Yang,et al. Acute Toxicity of Heavy Metals to Tetrahymena in an In Vitro Experiment and Envelope Damage Study[J]. BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY,2013,91(1):62-68.
APA Zhang, Tian.,Li, Xi.,Lu, Yang.,Wu, Chunyun.,Fang, Tingting.,...&Li, X .(2013).Acute Toxicity of Heavy Metals to Tetrahymena in an In Vitro Experiment and Envelope Damage Study.BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY,91(1),62-68.
MLA Zhang, Tian,et al."Acute Toxicity of Heavy Metals to Tetrahymena in an In Vitro Experiment and Envelope Damage Study".BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY 91.1(2013):62-68.
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