IHB OpenIR  > 水环境工程研究中心  > 期刊论文
Reproductive and Locomotory Capacities of Caenorhabditis elegans Were Not Affected by Simulated Variable Gravities and Spaceflight During the Shenzhou-8 Mission
Qiao, Liang1; Luo, Sang1; Liu, Yongding2; Li, Xiaoyan2; Wang, Gaohong2; Huang, Zebo1; Wang, GH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
2013-07-01
Source PublicationASTROBIOLOGY
ISSN1531-1074
Volume13Issue:7Pages:617-625
AbstractReproduction and locomotion are essential features of animals that help to facilitate their interaction with the surrounding environment. Previous studies have produced inconsistent results on behavioral response to spaceflight by the model animal Caenorhabditis elegans (C. elegans) in liquid culture. Using standard agar-based nematode growth medium (NGM), we show here that both reproductive and locomotory capacities of C. elegans were not significantly changed by centrifuge-produced hypergravity or clinostat-simulated microgravity. To investigate the effect of actual spaceflight on C. elegans, a nematode test unit was specifically designed to maintain its normal growth on solid NGM slides and to allow automatic RNA fixation on board the Shenzhou-8 spaceflight. We did not detect alteration in either brood size of immediate progenies from postflight nematodes or locomotory behavior, including speed of locomotion, frequency of reversals, and rate of body bends of space-flown nematodes collected directly from nematode test units. Our results provide clear evidence that the nematode test unit is an appropriate apparatus for nematode growth on standard NGM and can be used for on-orbit analysis of C. elegans, including onboard RNA fixation for molecular analysis and real-time video acquisition for behavioral analysis, which are critical for further studies in unmanned spaceflight and outer space exploration.; Reproduction and locomotion are essential features of animals that help to facilitate their interaction with the surrounding environment. Previous studies have produced inconsistent results on behavioral response to spaceflight by the model animal Caenorhabditis elegans (C. elegans) in liquid culture. Using standard agar-based nematode growth medium (NGM), we show here that both reproductive and locomotory capacities of C. elegans were not significantly changed by centrifuge-produced hypergravity or clinostat-simulated microgravity. To investigate the effect of actual spaceflight on C. elegans, a nematode test unit was specifically designed to maintain its normal growth on solid NGM slides and to allow automatic RNA fixation on board the Shenzhou-8 spaceflight. We did not detect alteration in either brood size of immediate progenies from postflight nematodes or locomotory behavior, including speed of locomotion, frequency of reversals, and rate of body bends of space-flown nematodes collected directly from nematode test units. Our results provide clear evidence that the nematode test unit is an appropriate apparatus for nematode growth on standard NGM and can be used for on-orbit analysis of C. elegans, including onboard RNA fixation for molecular analysis and real-time video acquisition for behavioral analysis, which are critical for further studies in unmanned spaceflight and outer space exploration.
SubtypeArticle
KeywordSpaceflight Hypergravity Microgravity Caenorhabditis Elegans Behavior Reproduction
Department[Qiao, Liang ; Luo, Sang ; Huang, Zebo] Wuhan Univ, Sch Pharmaceut Sci, Key Lab Combinatorial Biosynthesis & Drug Discove, Minist Educ, Wuhan 430071, Peoples R China ; [Liu, Yongding ; Li, Xiaoyan ; Wang, Gaohong] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
DOI10.1089/ast.2012.0962
WOS HeadingsScience & Technology ; Physical Sciences ; Life Sciences & Biomedicine
Funding OrganizationProject of Chinese Manned Spaceflight; National Natural Science Foundation of China [30970688, 81274048]; Fundamental Research Funds for the Central Universities [20083060101000071]; National Institutes of Health National Center for Research Resources ; Project of Chinese Manned Spaceflight; National Natural Science Foundation of China [30970688, 81274048]; Fundamental Research Funds for the Central Universities [20083060101000071]; National Institutes of Health National Center for Research Resources ; Project of Chinese Manned Spaceflight; National Natural Science Foundation of China [30970688, 81274048]; Fundamental Research Funds for the Central Universities [20083060101000071]; National Institutes of Health National Center for Research Resources ; Project of Chinese Manned Spaceflight; National Natural Science Foundation of China [30970688, 81274048]; Fundamental Research Funds for the Central Universities [20083060101000071]; National Institutes of Health National Center for Research Resources
Indexed BySCI
Language英语
WOS Research AreaAstronomy & Astrophysics ; Life Sciences & Biomedicine - Other Topics ; Geology
WOS SubjectAstronomy & Astrophysics ; Biology ; Geosciences, Multidisciplinary
WOS IDWOS:000321896100003
WOS KeywordLOW-EARTH-ORBIT ; RADIATION ; SPACE ; NEMATODES ; BEHAVIOR ; DESICCATION ; STRESS ; CELLS
Funding OrganizationProject of Chinese Manned Spaceflight; National Natural Science Foundation of China [30970688, 81274048]; Fundamental Research Funds for the Central Universities [20083060101000071]; National Institutes of Health National Center for Research Resources ; Project of Chinese Manned Spaceflight; National Natural Science Foundation of China [30970688, 81274048]; Fundamental Research Funds for the Central Universities [20083060101000071]; National Institutes of Health National Center for Research Resources ; Project of Chinese Manned Spaceflight; National Natural Science Foundation of China [30970688, 81274048]; Fundamental Research Funds for the Central Universities [20083060101000071]; National Institutes of Health National Center for Research Resources ; Project of Chinese Manned Spaceflight; National Natural Science Foundation of China [30970688, 81274048]; Fundamental Research Funds for the Central Universities [20083060101000071]; National Institutes of Health National Center for Research Resources
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19597
Collection水环境工程研究中心_期刊论文
Corresponding AuthorWang, GH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
Affiliation1.Wuhan Univ, Sch Pharmaceut Sci, Key Lab Combinatorial Biosynthesis & Drug Discove, Minist Educ, Wuhan 430071, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Qiao, Liang,Luo, Sang,Liu, Yongding,et al. Reproductive and Locomotory Capacities of Caenorhabditis elegans Were Not Affected by Simulated Variable Gravities and Spaceflight During the Shenzhou-8 Mission[J]. ASTROBIOLOGY,2013,13(7):617-625.
APA Qiao, Liang.,Luo, Sang.,Liu, Yongding.,Li, Xiaoyan.,Wang, Gaohong.,...&Wang, GH .(2013).Reproductive and Locomotory Capacities of Caenorhabditis elegans Were Not Affected by Simulated Variable Gravities and Spaceflight During the Shenzhou-8 Mission.ASTROBIOLOGY,13(7),617-625.
MLA Qiao, Liang,et al."Reproductive and Locomotory Capacities of Caenorhabditis elegans Were Not Affected by Simulated Variable Gravities and Spaceflight During the Shenzhou-8 Mission".ASTROBIOLOGY 13.7(2013):617-625.
Files in This Item:
File Name/Size DocType Version Access License
Reproductive and Loc(746KB) 开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Qiao, Liang]'s Articles
[Luo, Sang]'s Articles
[Liu, Yongding]'s Articles
Baidu academic
Similar articles in Baidu academic
[Qiao, Liang]'s Articles
[Luo, Sang]'s Articles
[Liu, Yongding]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Qiao, Liang]'s Articles
[Luo, Sang]'s Articles
[Liu, Yongding]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Reproductive and Locomotory Capacities of Caenorhabditis elegans Were Not Affected by Simulated Variable Gravities and Spaceflight During the Shenzhou-8 Mission.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.