| Reproductive and Locomotory Capacities of Caenorhabditis elegans Were Not Affected by Simulated Variable Gravities and Spaceflight During the Shenzhou-8 Mission | |
| Qiao, Liang1; Luo, Sang1; Liu, Yongding2; Li, Xiaoyan2; Wang, Gaohong2; Huang, Zebo1; Wang, GH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China. | |
| 2013-07-01 | |
| Source Publication | ASTROBIOLOGY
 |
| ISSN | 1531-1074 |
| Volume | 13Issue:7Pages:617-625 |
| Abstract | Reproduction and locomotion are essential features of animals that help to facilitate their interaction with the surrounding environment. Previous studies have produced inconsistent results on behavioral response to spaceflight by the model animal Caenorhabditis elegans (C. elegans) in liquid culture. Using standard agar-based nematode growth medium (NGM), we show here that both reproductive and locomotory capacities of C. elegans were not significantly changed by centrifuge-produced hypergravity or clinostat-simulated microgravity. To investigate the effect of actual spaceflight on C. elegans, a nematode test unit was specifically designed to maintain its normal growth on solid NGM slides and to allow automatic RNA fixation on board the Shenzhou-8 spaceflight. We did not detect alteration in either brood size of immediate progenies from postflight nematodes or locomotory behavior, including speed of locomotion, frequency of reversals, and rate of body bends of space-flown nematodes collected directly from nematode test units. Our results provide clear evidence that the nematode test unit is an appropriate apparatus for nematode growth on standard NGM and can be used for on-orbit analysis of C. elegans, including onboard RNA fixation for molecular analysis and real-time video acquisition for behavioral analysis, which are critical for further studies in unmanned spaceflight and outer space exploration.; Reproduction and locomotion are essential features of animals that help to facilitate their interaction with the surrounding environment. Previous studies have produced inconsistent results on behavioral response to spaceflight by the model animal Caenorhabditis elegans (C. elegans) in liquid culture. Using standard agar-based nematode growth medium (NGM), we show here that both reproductive and locomotory capacities of C. elegans were not significantly changed by centrifuge-produced hypergravity or clinostat-simulated microgravity. To investigate the effect of actual spaceflight on C. elegans, a nematode test unit was specifically designed to maintain its normal growth on solid NGM slides and to allow automatic RNA fixation on board the Shenzhou-8 spaceflight. We did not detect alteration in either brood size of immediate progenies from postflight nematodes or locomotory behavior, including speed of locomotion, frequency of reversals, and rate of body bends of space-flown nematodes collected directly from nematode test units. Our results provide clear evidence that the nematode test unit is an appropriate apparatus for nematode growth on standard NGM and can be used for on-orbit analysis of C. elegans, including onboard RNA fixation for molecular analysis and real-time video acquisition for behavioral analysis, which are critical for further studies in unmanned spaceflight and outer space exploration. |
| Subtype | Article |
| Keyword | Spaceflight Hypergravity Microgravity Caenorhabditis Elegans Behavior Reproduction |
| Department | [Qiao, Liang ; Luo, Sang ; Huang, Zebo] Wuhan Univ, Sch Pharmaceut Sci, Key Lab Combinatorial Biosynthesis & Drug Discove, Minist Educ, Wuhan 430071, Peoples R China ; [Liu, Yongding ; Li, Xiaoyan ; Wang, Gaohong] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| DOI | 10.1089/ast.2012.0962 |
| WOS Headings | Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine |
| Funding Organization | Project of Chinese Manned Spaceflight; National Natural Science Foundation of China [30970688, 81274048]; Fundamental Research Funds for the Central Universities [20083060101000071]; National Institutes of Health National Center for Research Resources ; Project of Chinese Manned Spaceflight; National Natural Science Foundation of China [30970688, 81274048]; Fundamental Research Funds for the Central Universities [20083060101000071]; National Institutes of Health National Center for Research Resources ; Project of Chinese Manned Spaceflight; National Natural Science Foundation of China [30970688, 81274048]; Fundamental Research Funds for the Central Universities [20083060101000071]; National Institutes of Health National Center for Research Resources ; Project of Chinese Manned Spaceflight; National Natural Science Foundation of China [30970688, 81274048]; Fundamental Research Funds for the Central Universities [20083060101000071]; National Institutes of Health National Center for Research Resources |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Astronomy & Astrophysics ; Life Sciences & Biomedicine - Other Topics ; Geology |
| WOS Subject | Astronomy & Astrophysics ; Biology ; Geosciences, Multidisciplinary |
| WOS ID | WOS:000321896100003 |
| WOS Keyword | LOW-EARTH-ORBIT ; RADIATION ; SPACE ; NEMATODES ; BEHAVIOR ; DESICCATION ; STRESS ; CELLS |
| Funding Organization | Project of Chinese Manned Spaceflight; National Natural Science Foundation of China [30970688, 81274048]; Fundamental Research Funds for the Central Universities [20083060101000071]; National Institutes of Health National Center for Research Resources ; Project of Chinese Manned Spaceflight; National Natural Science Foundation of China [30970688, 81274048]; Fundamental Research Funds for the Central Universities [20083060101000071]; National Institutes of Health National Center for Research Resources ; Project of Chinese Manned Spaceflight; National Natural Science Foundation of China [30970688, 81274048]; Fundamental Research Funds for the Central Universities [20083060101000071]; National Institutes of Health National Center for Research Resources ; Project of Chinese Manned Spaceflight; National Natural Science Foundation of China [30970688, 81274048]; Fundamental Research Funds for the Central Universities [20083060101000071]; National Institutes of Health National Center for Research Resources |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/19597 |
| Collection | 水环境工程研究中心_期刊论文 |
| Corresponding Author | Wang, GH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China. |
| Affiliation | 1.Wuhan Univ, Sch Pharmaceut Sci, Key Lab Combinatorial Biosynthesis & Drug Discove, Minist Educ, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Qiao, Liang,Luo, Sang,Liu, Yongding,et al. Reproductive and Locomotory Capacities of Caenorhabditis elegans Were Not Affected by Simulated Variable Gravities and Spaceflight During the Shenzhou-8 Mission[J]. ASTROBIOLOGY,2013,13(7):617-625. |
| APA | Qiao, Liang.,Luo, Sang.,Liu, Yongding.,Li, Xiaoyan.,Wang, Gaohong.,...&Wang, GH .(2013).Reproductive and Locomotory Capacities of Caenorhabditis elegans Were Not Affected by Simulated Variable Gravities and Spaceflight During the Shenzhou-8 Mission.ASTROBIOLOGY,13(7),617-625. |
| MLA | Qiao, Liang,et al."Reproductive and Locomotory Capacities of Caenorhabditis elegans Were Not Affected by Simulated Variable Gravities and Spaceflight During the Shenzhou-8 Mission".ASTROBIOLOGY 13.7(2013):617-625. |
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