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题名: Reproductive and Locomotory Capacities of Caenorhabditis elegans Were Not Affected by Simulated Variable Gravities and Spaceflight During the Shenzhou-8 Mission
作者: Qiao, Liang1; Luo, Sang1; Liu, Yongding2; Li, Xiaoyan2; Wang, Gaohong2; Huang, Zebo1
通讯作者: Wang, GH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
关键词: Spaceflight ; Hypergravity ; Microgravity ; Caenorhabditis elegans ; Behavior ; Reproduction
刊名: ASTROBIOLOGY
发表日期: 2013-07-01
DOI: 10.1089/ast.2012.0962
卷: 13, 期:7, 页:617-625
收录类别: SCI
文章类型: Article
部门归属: [Qiao, Liang ; Luo, Sang ; Huang, Zebo] Wuhan Univ, Sch Pharmaceut Sci, Key Lab Combinatorial Biosynthesis & Drug Discove, Minist Educ, Wuhan 430071, Peoples R China ; [Liu, Yongding ; Li, Xiaoyan ; Wang, Gaohong] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine
资助者: Project of Chinese Manned Spaceflight; National Natural Science Foundation of China [30970688, 81274048]; Fundamental Research Funds for the Central Universities [20083060101000071]; National Institutes of Health National Center for Research Resources
类目[WOS]: Astronomy & Astrophysics ; Biology ; Geosciences, Multidisciplinary
研究领域[WOS]: Astronomy & Astrophysics ; Life Sciences & Biomedicine - Other Topics ; Geology
摘要: Reproduction and locomotion are essential features of animals that help to facilitate their interaction with the surrounding environment. Previous studies have produced inconsistent results on behavioral response to spaceflight by the model animal Caenorhabditis elegans (C. elegans) in liquid culture. Using standard agar-based nematode growth medium (NGM), we show here that both reproductive and locomotory capacities of C. elegans were not significantly changed by centrifuge-produced hypergravity or clinostat-simulated microgravity. To investigate the effect of actual spaceflight on C. elegans, a nematode test unit was specifically designed to maintain its normal growth on solid NGM slides and to allow automatic RNA fixation on board the Shenzhou-8 spaceflight. We did not detect alteration in either brood size of immediate progenies from postflight nematodes or locomotory behavior, including speed of locomotion, frequency of reversals, and rate of body bends of space-flown nematodes collected directly from nematode test units. Our results provide clear evidence that the nematode test unit is an appropriate apparatus for nematode growth on standard NGM and can be used for on-orbit analysis of C. elegans, including onboard RNA fixation for molecular analysis and real-time video acquisition for behavioral analysis, which are critical for further studies in unmanned spaceflight and outer space exploration.
英文摘要: Reproduction and locomotion are essential features of animals that help to facilitate their interaction with the surrounding environment. Previous studies have produced inconsistent results on behavioral response to spaceflight by the model animal Caenorhabditis elegans (C. elegans) in liquid culture. Using standard agar-based nematode growth medium (NGM), we show here that both reproductive and locomotory capacities of C. elegans were not significantly changed by centrifuge-produced hypergravity or clinostat-simulated microgravity. To investigate the effect of actual spaceflight on C. elegans, a nematode test unit was specifically designed to maintain its normal growth on solid NGM slides and to allow automatic RNA fixation on board the Shenzhou-8 spaceflight. We did not detect alteration in either brood size of immediate progenies from postflight nematodes or locomotory behavior, including speed of locomotion, frequency of reversals, and rate of body bends of space-flown nematodes collected directly from nematode test units. Our results provide clear evidence that the nematode test unit is an appropriate apparatus for nematode growth on standard NGM and can be used for on-orbit analysis of C. elegans, including onboard RNA fixation for molecular analysis and real-time video acquisition for behavioral analysis, which are critical for further studies in unmanned spaceflight and outer space exploration.
关键词[WOS]: LOW-EARTH-ORBIT ; RADIATION ; SPACE ; NEMATODES ; BEHAVIOR ; DESICCATION ; STRESS ; CELLS
语种: 英语
WOS记录号: WOS:000321896100003
ISSN号: 1531-1074
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19597
Appears in Collections:水环境工程研究中心_期刊论文

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作者单位: 1.Wuhan Univ, Sch Pharmaceut Sci, Key Lab Combinatorial Biosynthesis & Drug Discove, Minist Educ, Wuhan 430071, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

Recommended Citation:
Qiao, Liang; Luo, Sang; Liu, Yongding; Li, Xiaoyan; Wang, Gaohong; Huang, Zebo.Reproductive and Locomotory Capacities of Caenorhabditis elegans Were Not Affected by Simulated Variable Gravities and Spaceflight During the Shenzhou-8 Mission,ASTROBIOLOGY,2013,13(7):617-625
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