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Endocrine disruption by di-(2-ethylhexyl)-phthalate in Chinese rare minnow (Gobiocypris rarus)
Wang, Xiaofang1,2; Yang, Yuanjin3; Zhang, Liping1; Ma, Yanbo1; Han, Jian1; Yang, Lihua1; Zhou, Bingsheng1; Zhou, BS (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China.
2013-08-01
Source PublicationENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
ISSN0730-7268
Volume32Issue:8Pages:1846-1854
AbstractGreat concern has been raised over the potential impact of environmental contaminants on fish populations that inhabit the Three Gorge Reservoir. The present study investigated the endocrine-disrupting effects of di-(2-ethylhexyl)-phthalate (DEHP) on the Chinese rare minnow (Gobiocypris rarus), an endemic fish distributed in upstream waters in the Yangtze River. Adult rare minnow were exposed to environmentally relevant concentrations of DEHP (0 mu g/L, 3.6 mu g/L, 12.8 mu g/L, 39.4 mu g/L, and 117.6 mu g/L) for a 21-d period. Then, concentrations of sex hormones in the plasma and relative transcription of various associated genes were measured in the hypothalamic-pituitary-gonadal (HPG) axis and liver of the fish. Exposure to DEHP resulted in greater circulating concentrations of testosterone (T) and lower concentrations of estradiol (E2), which were accompanied by upregulation of Cyp17 mRNA and downregulation of Cyp19a mRNA in the gonads of females. In males, increases of T and E2 levels were consistent with upregulation of Cyp17 and Cyp19a in the gonads. Furthermore, the T/E2 ratio was increased in females but reduced in males. A significant increase in the levels of hepatic vitellogenin (VTG) gene transcription was observed in both females and males. The present study showed that waterborne exposure to DEHP altered plasma sex hormone levels and modulated gene transcription profiles of associated genes in the HPG axis and liver, occurring mostly at higher concentrations (>39.4 mu g/L), which suggests that environmental concentration of DEHP (5.4 mu g/L) alone might not disturb the endocrine system of the rare minnow in the TGR. Environ Toxicol Chem 2013;32:1846-1854. (c) 2013 SETAC; Great concern has been raised over the potential impact of environmental contaminants on fish populations that inhabit the Three Gorge Reservoir. The present study investigated the endocrine-disrupting effects of di-(2-ethylhexyl)-phthalate (DEHP) on the Chinese rare minnow (Gobiocypris rarus), an endemic fish distributed in upstream waters in the Yangtze River. Adult rare minnow were exposed to environmentally relevant concentrations of DEHP (0 mu g/L, 3.6 mu g/L, 12.8 mu g/L, 39.4 mu g/L, and 117.6 mu g/L) for a 21-d period. Then, concentrations of sex hormones in the plasma and relative transcription of various associated genes were measured in the hypothalamic-pituitary-gonadal (HPG) axis and liver of the fish. Exposure to DEHP resulted in greater circulating concentrations of testosterone (T) and lower concentrations of estradiol (E2), which were accompanied by upregulation of Cyp17 mRNA and downregulation of Cyp19a mRNA in the gonads of females. In males, increases of T and E2 levels were consistent with upregulation of Cyp17 and Cyp19a in the gonads. Furthermore, the T/E2 ratio was increased in females but reduced in males. A significant increase in the levels of hepatic vitellogenin (VTG) gene transcription was observed in both females and males. The present study showed that waterborne exposure to DEHP altered plasma sex hormone levels and modulated gene transcription profiles of associated genes in the HPG axis and liver, occurring mostly at higher concentrations (>39.4 mu g/L), which suggests that environmental concentration of DEHP (5.4 mu g/L) alone might not disturb the endocrine system of the rare minnow in the TGR. Environ Toxicol Chem 2013;32:1846-1854. (c) 2013 SETAC
SubtypeArticle
KeywordDi-(2-ethylhexyl)-phthalate (Dehp) Hypothalamic-pituitary-gonadal (Hpg) Axis Sex Hormones Endocrine Disruption Chinese Rare Minnow
Department[Wang, Xiaofang ; Zhang, Liping ; Ma, Yanbo ; Han, Jian ; Yang, Lihua ; Zhou, Bingsheng] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China ; [Wang, Xiaofang] Univ Chinese Acad Sci, Beijing, Peoples R China ; [Yang, Yuanjin] China Three Gorges Corp, Inst Chinese Sturgeon Res, Yichang, Hubei, Peoples R China
DOI10.1002/etc.2261
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationScience and Technology Research Project of China Three Gorges Corporation [07011034] ; Science and Technology Research Project of China Three Gorges Corporation [07011034] ; Science and Technology Research Project of China Three Gorges Corporation [07011034] ; Science and Technology Research Project of China Three Gorges Corporation [07011034]
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Toxicology
WOS SubjectEnvironmental Sciences ; Toxicology
WOS IDWOS:000321549200022
WOS KeywordPHTHALIC-ACID ESTERS ; PITUITARY-GONADAL AXIS ; GENE-EXPRESSION ; PIMEPHALES-PROMELAS ; CYPRINUS-CARPIO ; FATHEAD MINNOWS ; BISPHENOL-A ; TOXICITY ; RECEPTOR ; RATS
Funding OrganizationScience and Technology Research Project of China Three Gorges Corporation [07011034] ; Science and Technology Research Project of China Three Gorges Corporation [07011034] ; Science and Technology Research Project of China Three Gorges Corporation [07011034] ; Science and Technology Research Project of China Three Gorges Corporation [07011034]
Citation statistics
Cited Times:32[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19595
Collection水环境工程研究中心_期刊论文
Corresponding AuthorZhou, BS (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.China Three Gorges Corp, Inst Chinese Sturgeon Res, Yichang, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Wang, Xiaofang,Yang, Yuanjin,Zhang, Liping,et al. Endocrine disruption by di-(2-ethylhexyl)-phthalate in Chinese rare minnow (Gobiocypris rarus)[J]. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY,2013,32(8):1846-1854.
APA Wang, Xiaofang.,Yang, Yuanjin.,Zhang, Liping.,Ma, Yanbo.,Han, Jian.,...&Zhou, BS .(2013).Endocrine disruption by di-(2-ethylhexyl)-phthalate in Chinese rare minnow (Gobiocypris rarus).ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY,32(8),1846-1854.
MLA Wang, Xiaofang,et al."Endocrine disruption by di-(2-ethylhexyl)-phthalate in Chinese rare minnow (Gobiocypris rarus)".ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY 32.8(2013):1846-1854.
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