It has previously been shown that the Shewanella putrefaciens W3-18-1 strain produces remarkably high current in microbial fuel cells (MFCs) and can form magnetite at 0 degrees C. To explore the underlying mechanisms, we developed a genetic manipulation method by deleting the restriction-modification system genes of the SGI1 (Salmonella genome island 1)-like prophage and analyzed the key genes involved in bacterial respiration. W3-18-1 has less respiratory flexibility than the well-characterized S. oneidensis MR-1 strain, as it possesses fewer cytochrome c genes and lacks the ability to oxidize sulfite or reduce dimethyl sulfoxide (DMSO) and timethylamine oxide (TMAO). W3-18-1 lacks the hydrogen-producing Fe-only hydrogenase, and the hydrogen-oxidizing Ni-Fe hydrogenase genes were split into two separate clusters. Two periplasmic nitrate reductases (NapDAGHB and NapDABC) were functionally redundant in anaerobic growth of W3-18-1 with nitrate as the electron acceptor, though napDABC was not regulated by Crp. Moreover, nitrate respiration started earlier in W3-18-1 than in MR-1 (with NapDAGHB only) under microoxic conditions. These results indicate that Shewanella putrefaciens W3-18-1 is well adapted to habitats with higher oxygen levels. Taken together, the results of this study provide valuable insights into bacterial genome evolution.
英文摘要:
It has previously been shown that the Shewanella putrefaciens W3-18-1 strain produces remarkably high current in microbial fuel cells (MFCs) and can form magnetite at 0 degrees C. To explore the underlying mechanisms, we developed a genetic manipulation method by deleting the restriction-modification system genes of the SGI1 (Salmonella genome island 1)-like prophage and analyzed the key genes involved in bacterial respiration. W3-18-1 has less respiratory flexibility than the well-characterized S. oneidensis MR-1 strain, as it possesses fewer cytochrome c genes and lacks the ability to oxidize sulfite or reduce dimethyl sulfoxide (DMSO) and timethylamine oxide (TMAO). W3-18-1 lacks the hydrogen-producing Fe-only hydrogenase, and the hydrogen-oxidizing Ni-Fe hydrogenase genes were split into two separate clusters. Two periplasmic nitrate reductases (NapDAGHB and NapDABC) were functionally redundant in anaerobic growth of W3-18-1 with nitrate as the electron acceptor, though napDABC was not regulated by Crp. Moreover, nitrate respiration started earlier in W3-18-1 than in MR-1 (with NapDAGHB only) under microoxic conditions. These results indicate that Shewanella putrefaciens W3-18-1 is well adapted to habitats with higher oxygen levels. Taken together, the results of this study provide valuable insights into bacterial genome evolution.
关键词[WOS]:
C-TYPE CYTOCHROME
; PERIPLASMIC NITRATE REDUCTASE
; ONEIDENSIS MR-1
; ANAEROBIC RESPIRATION
; FUMARATE REDUCTASE
; ESCHERICHIA-COLI
; PSEUDOMONAS-AERUGINOSA
; GENUS SHEWANELLA
; SYSTEMS BIOLOGY
; SP. NOV
语种:
英语
项目资助者:
U.S. Department of Energy (DOE)(DE-FG02-07ER64383)
; Chinese Academy of Science(Y15103-1-401)
; ENIGMA under the Office of Science, Office of Biological and Environmental Research, of the U.S. Department of Energy(DE-AC02-05CH11231)
1.Univ Oklahoma, Inst Environm Genom, Norman, OK 73019 USA 2.Univ Oklahoma, Dept Microbiol & Plant Biol, Norman, OK 73019 USA 3.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China 4.Chinese Acad Sci, Grad Univ, Wuhan, Peoples R China 5.Tsinghua Univ, Sch Environm, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100084, Peoples R China 6.Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Div Earth Sci, Berkeley, CA 94720 USA
Recommended Citation:
Qiu, Dongru; Wei, Hehong; Tu, Qichao; Yang, Yunfeng; Xie, Ming; Chen, Jingrong; Pinkerton, Mark H., Jr.; Liang, Yili; He, Zhili; Zhou, Jizhong.Combined Genomics and Experimental Analyses of Respiratory Characteristics of Shewanella putrefaciens W3-18-1,APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2013,79(17):5250-5257
