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题名: Combined Genomics and Experimental Analyses of Respiratory Characteristics of Shewanella putrefaciens W3-18-1
作者: Qiu, Dongru1, 2, 3, 4; Wei, Hehong3, 4; Tu, Qichao1, 2; Yang, Yunfeng5, 6; Xie, Ming1, 2; Chen, Jingrong1, 2; Pinkerton, Mark H., Jr.1, 2; Liang, Yili1, 2; He, Zhili1, 2; Zhou, Jizhong1, 2
通讯作者: Zhou, JZ (reprint author), Univ Oklahoma, Inst Environm Genom, Norman, OK 73019 USA.
关键词: C-TYPE CYTOCHROME ; PERIPLASMIC NITRATE REDUCTASE ; ONEIDENSIS MR-1 ; ANAEROBIC RESPIRATION ; FUMARATE REDUCTASE ; ESCHERICHIA-COLI ; PSEUDOMONAS-AERUGINOSA ; GENUS SHEWANELLA ; SYSTEMS BIOLOGY ; SP. NOV
刊名: APPLIED AND ENVIRONMENTAL MICROBIOLOGY
发表日期: 2013-09-01
DOI: 10.1128/AEM.00619-13
卷: 79, 期:17, 页:5250-5257
收录类别: SCI
文章类型: Article
部门归属: [Qiu, Dongru ; Tu, Qichao ; Xie, Ming ; Chen, Jingrong ; Pinkerton, Mark H., Jr. ; Liang, Yili ; He, Zhili ; Zhou, Jizhong] Univ Oklahoma, Inst Environm Genom, Norman, OK 73019 USA ; [Qiu, Dongru ; Tu, Qichao ; Xie, Ming ; Chen, Jingrong ; Pinkerton, Mark H., Jr. ; Liang, Yili ; He, Zhili ; Zhou, Jizhong] Univ Oklahoma, Dept Microbiol & Plant Biol, Norman, OK 73019 USA ; [Qiu, Dongru ; Wei, Hehong] Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China ; [Qiu, Dongru ; Wei, Hehong] Chinese Acad Sci, Grad Univ, Wuhan, Peoples R China ; [Yang, Yunfeng] Tsinghua Univ, Sch Environm, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100084, Peoples R China ; [Yang, Yunfeng] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Div Earth Sci, Berkeley, CA 94720 USA
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: U.S. Department of Energy (DOE) [DE-FG02-07ER64383]; Chinese Academy of Science [Y15103-1-401]; ENIGMA under the Office of Science, Office of Biological and Environmental Research, of the U.S. Department of Energy [DE-AC02-05CH11231]
类目[WOS]: Biotechnology & Applied Microbiology ; Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology ; Microbiology
摘要: It has previously been shown that the Shewanella putrefaciens W3-18-1 strain produces remarkably high current in microbial fuel cells (MFCs) and can form magnetite at 0 degrees C. To explore the underlying mechanisms, we developed a genetic manipulation method by deleting the restriction-modification system genes of the SGI1 (Salmonella genome island 1)-like prophage and analyzed the key genes involved in bacterial respiration. W3-18-1 has less respiratory flexibility than the well-characterized S. oneidensis MR-1 strain, as it possesses fewer cytochrome c genes and lacks the ability to oxidize sulfite or reduce dimethyl sulfoxide (DMSO) and timethylamine oxide (TMAO). W3-18-1 lacks the hydrogen-producing Fe-only hydrogenase, and the hydrogen-oxidizing Ni-Fe hydrogenase genes were split into two separate clusters. Two periplasmic nitrate reductases (NapDAGHB and NapDABC) were functionally redundant in anaerobic growth of W3-18-1 with nitrate as the electron acceptor, though napDABC was not regulated by Crp. Moreover, nitrate respiration started earlier in W3-18-1 than in MR-1 (with NapDAGHB only) under microoxic conditions. These results indicate that Shewanella putrefaciens W3-18-1 is well adapted to habitats with higher oxygen levels. Taken together, the results of this study provide valuable insights into bacterial genome evolution.
英文摘要: It has previously been shown that the Shewanella putrefaciens W3-18-1 strain produces remarkably high current in microbial fuel cells (MFCs) and can form magnetite at 0 degrees C. To explore the underlying mechanisms, we developed a genetic manipulation method by deleting the restriction-modification system genes of the SGI1 (Salmonella genome island 1)-like prophage and analyzed the key genes involved in bacterial respiration. W3-18-1 has less respiratory flexibility than the well-characterized S. oneidensis MR-1 strain, as it possesses fewer cytochrome c genes and lacks the ability to oxidize sulfite or reduce dimethyl sulfoxide (DMSO) and timethylamine oxide (TMAO). W3-18-1 lacks the hydrogen-producing Fe-only hydrogenase, and the hydrogen-oxidizing Ni-Fe hydrogenase genes were split into two separate clusters. Two periplasmic nitrate reductases (NapDAGHB and NapDABC) were functionally redundant in anaerobic growth of W3-18-1 with nitrate as the electron acceptor, though napDABC was not regulated by Crp. Moreover, nitrate respiration started earlier in W3-18-1 than in MR-1 (with NapDAGHB only) under microoxic conditions. These results indicate that Shewanella putrefaciens W3-18-1 is well adapted to habitats with higher oxygen levels. Taken together, the results of this study provide valuable insights into bacterial genome evolution.
关键词[WOS]: C-TYPE CYTOCHROME ; PERIPLASMIC NITRATE REDUCTASE ; ONEIDENSIS MR-1 ; ANAEROBIC RESPIRATION ; FUMARATE REDUCTASE ; ESCHERICHIA-COLI ; PSEUDOMONAS-AERUGINOSA ; GENUS SHEWANELLA ; SYSTEMS BIOLOGY ; SP. NOV
语种: 英语
项目资助者: U.S. Department of Energy (DOE)(DE-FG02-07ER64383) ; Chinese Academy of Science(Y15103-1-401) ; ENIGMA under the Office of Science, Office of Biological and Environmental Research, of the U.S. Department of Energy(DE-AC02-05CH11231)
WOS记录号: WOS:000322828100019
ISSN号: 0099-2240
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19593
Appears in Collections:水环境工程研究中心_期刊论文

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作者单位: 1.Univ Oklahoma, Inst Environm Genom, Norman, OK 73019 USA
2.Univ Oklahoma, Dept Microbiol & Plant Biol, Norman, OK 73019 USA
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
4.Chinese Acad Sci, Grad Univ, Wuhan, Peoples R China
5.Tsinghua Univ, Sch Environm, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100084, Peoples R China
6.Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Div Earth Sci, Berkeley, CA 94720 USA

Recommended Citation:
Qiu, Dongru; Wei, Hehong; Tu, Qichao; Yang, Yunfeng; Xie, Ming; Chen, Jingrong; Pinkerton, Mark H., Jr.; Liang, Yili; He, Zhili; Zhou, Jizhong.Combined Genomics and Experimental Analyses of Respiratory Characteristics of Shewanella putrefaciens W3-18-1,APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2013,79(17):5250-5257
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