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Identification of a Novel Gig2 Gene Family Specific to Non-Amniote Vertebrates
Zhang, Yi-Bing; Liu, Ting-Kai; Jiang, Jun; Shi, Jun; Liu, Ying; Li, Shun; Gui, Jian-Fang; Zhang, YB (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China.
2013-04-04
Source PublicationPLOS ONE
ISSN1932-6203
Volume8Issue:4Pages:e60588
AbstractGig2 (grass carp reovirus (GCRV)-induced gene 2) is first identified as a novel fish interferon (IFN)-stimulated gene (ISG). Overexpression of a zebrafish Gig2 gene can protect cultured fish cells from virus infection. In the present study, we identify a novel gene family that is comprised of genes homologous to the previously characterized Gig2. EST/GSS search and in silico cloning identify 190 Gig2 homologous genes in 51 vertebrate species ranged from lampreys to amphibians. Further large-scale search of vertebrate and invertebrate genome databases indicate that Gig2 gene family is specific to non-amniotes including lampreys, sharks/rays, ray-finned fishes and amphibians. Phylogenetic analysis and synteny analysis reveal lineage-specific expansion of Gig2 gene family and also provide valuable evidence for the fish-specific genome duplication (FSGD) hypothesis. Although Gig2 family proteins exhibit no significant sequence similarity to any known proteins, a typical Gig2 protein appears to consist of two conserved parts: an N-terminus that bears very low homology to the catalytic domains of poly(ADP-ribose) polymerases (PARPs), and a novel C-terminal domain that is unique to this gene family. Expression profiling of zebrafish Gig2 family genes shows that some duplicate pairs have diverged in function via acquisition of novel spatial and/or temporal expression under stresses. The specificity of this gene family to non-amniotes might contribute to a large extent to distinct physiology in non-amniote vertebrates.; Gig2 (grass carp reovirus (GCRV)-induced gene 2) is first identified as a novel fish interferon (IFN)-stimulated gene (ISG). Overexpression of a zebrafish Gig2 gene can protect cultured fish cells from virus infection. In the present study, we identify a novel gene family that is comprised of genes homologous to the previously characterized Gig2. EST/GSS search and in silico cloning identify 190 Gig2 homologous genes in 51 vertebrate species ranged from lampreys to amphibians. Further large-scale search of vertebrate and invertebrate genome databases indicate that Gig2 gene family is specific to non-amniotes including lampreys, sharks/rays, ray-finned fishes and amphibians. Phylogenetic analysis and synteny analysis reveal lineage-specific expansion of Gig2 gene family and also provide valuable evidence for the fish-specific genome duplication (FSGD) hypothesis. Although Gig2 family proteins exhibit no significant sequence similarity to any known proteins, a typical Gig2 protein appears to consist of two conserved parts: an N-terminus that bears very low homology to the catalytic domains of poly(ADP-ribose) polymerases (PARPs), and a novel C-terminal domain that is unique to this gene family. Expression profiling of zebrafish Gig2 family genes shows that some duplicate pairs have diverged in function via acquisition of novel spatial and/or temporal expression under stresses. The specificity of this gene family to non-amniotes might contribute to a large extent to distinct physiology in non-amniote vertebrates.
SubtypeArticle
KeywordDependent Protein-kinase Atlantic Salmon Genome Duplication Antiviral Response Teleost Fish Cell-line Evolution Rna Virus Poly(Adp-ribose)
Department[Zhang, Yi-Bing ; Liu, Ting-Kai ; Jiang, Jun ; Shi, Jun ; Liu, Ying ; Li, Shun ; Gui, Jian-Fang] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
DOI10.1371/journal.pone.0060588
WOS HeadingsScience & Technology
Funding Organization973 National Basic Research Program of China [2010CB126303]; National Natural Science Foundation of China [31272690, 31172435] ; 973 National Basic Research Program of China [2010CB126303]; National Natural Science Foundation of China [31272690, 31172435] ; 973 National Basic Research Program of China [2010CB126303]; National Natural Science Foundation of China [31272690, 31172435] ; 973 National Basic Research Program of China [2010CB126303]; National Natural Science Foundation of China [31272690, 31172435]
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000319108100056
WOS KeywordDEPENDENT PROTEIN-KINASE ; ATLANTIC SALMON ; GENOME DUPLICATION ; ANTIVIRAL RESPONSE ; TELEOST FISH ; CELL-LINE ; EVOLUTION ; RNA ; VIRUS ; POLY(ADP-RIBOSE)
Funding Organization973 National Basic Research Program of China [2010CB126303]; National Natural Science Foundation of China [31272690, 31172435] ; 973 National Basic Research Program of China [2010CB126303]; National Natural Science Foundation of China [31272690, 31172435] ; 973 National Basic Research Program of China [2010CB126303]; National Natural Science Foundation of China [31272690, 31172435] ; 973 National Basic Research Program of China [2010CB126303]; National Natural Science Foundation of China [31272690, 31172435]
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Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19587
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorZhang, YB (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China.
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Yi-Bing,Liu, Ting-Kai,Jiang, Jun,et al. Identification of a Novel Gig2 Gene Family Specific to Non-Amniote Vertebrates[J]. PLOS ONE,2013,8(4):e60588.
APA Zhang, Yi-Bing.,Liu, Ting-Kai.,Jiang, Jun.,Shi, Jun.,Liu, Ying.,...&Zhang, YB .(2013).Identification of a Novel Gig2 Gene Family Specific to Non-Amniote Vertebrates.PLOS ONE,8(4),e60588.
MLA Zhang, Yi-Bing,et al."Identification of a Novel Gig2 Gene Family Specific to Non-Amniote Vertebrates".PLOS ONE 8.4(2013):e60588.
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