IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Nonstructural Protein NS80 Is Crucial in Recruiting Viral Components to Form Aquareoviral Factories
Ke, Fei; He, Li-Bo; Zhang, Qi-Ya; Zhang, QY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China.
2013-05-06
Source PublicationPLOS ONE
ISSN1932-6203
Volume8Issue:5Pages:e63737
AbstractBackground: Replication and assembly of vertebrate reoviruses occur in specific intracellular compartments known as viral factories. Recently, NS88 and NS80, the nonstructural proteins from aquareoviruses, have been proposed to share common traits with mu NS from orthoreoviruses, which are involved in the formation of viral factories. Methodology/Principal Findings: In this study, the NS80 characteristics and its interactions with other viral components were investigated. We observed that the NS80 structure ensured its self-aggregation and selective recruitment of viral proteins to viral factories like structures (VFLS). The minimum amino acids (aa) of NS80 required for VFLS formation included 193 aa at the C-terminal. However, this truncated protein only contained one aa coil and located in the nucleus. Its N-terminal residual regions, aa 1-55 and aa 55-85, were required for recruiting viral nonstructural protein NS38 and structural protein VP3, respectively. A conserved N-terminal region of NS38, which was responsible for the interaction with NS80, was also identified. Moreover, the minimal region of C-terminal residues, aa 506-742 (Delta 505), required for NS80 self-aggregation in the cytoplasm, and aa 550-742 (Delta 549), which are sufficient for recruiting viral structure proteins VP1, VP2, and VP4 were also identified. Conclusions/Significance: The present study shows detailed interactions between NS80 and NS38 or other viral proteins. Sequence and structure characteristics of NS80 ensures its self-aggregation to form VFLS (either in the cytoplasm or nucleus) and recruitment of viral structural or nonstructural proteins.; Background: Replication and assembly of vertebrate reoviruses occur in specific intracellular compartments known as viral factories. Recently, NS88 and NS80, the nonstructural proteins from aquareoviruses, have been proposed to share common traits with mu NS from orthoreoviruses, which are involved in the formation of viral factories.
SubtypeArticle
KeywordRana-grylio-virus Grass Carp Reovirus Mu-ns Sigma-ns Fish Cells Paralichthys-olivaceus Functional Domains Genus Aquareovirus Family Reoviridae Inclusions
Department[Ke, Fei ; He, Li-Bo ; Zhang, Qi-Ya] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
DOI10.1371/journal.pone.0063737
WOS HeadingsScience & Technology
Funding OrganizationNational Major Basic Research Program [2009CB118704, 2010CB126303]; National Natural Science Foundation of China [30871938, 31072239]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ12, 2013FB13] ; National Major Basic Research Program [2009CB118704, 2010CB126303]; National Natural Science Foundation of China [30871938, 31072239]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ12, 2013FB13] ; National Major Basic Research Program [2009CB118704, 2010CB126303]; National Natural Science Foundation of China [30871938, 31072239]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ12, 2013FB13] ; National Major Basic Research Program [2009CB118704, 2010CB126303]; National Natural Science Foundation of China [30871938, 31072239]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ12, 2013FB13]
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000321390200059
WOS KeywordRANA-GRYLIO-VIRUS ; GRASS CARP REOVIRUS ; MU-NS ; SIGMA-NS ; FISH CELLS ; PARALICHTHYS-OLIVACEUS ; FUNCTIONAL DOMAINS ; GENUS AQUAREOVIRUS ; FAMILY REOVIRIDAE ; INCLUSIONS
Funding OrganizationNational Major Basic Research Program [2009CB118704, 2010CB126303]; National Natural Science Foundation of China [30871938, 31072239]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ12, 2013FB13] ; National Major Basic Research Program [2009CB118704, 2010CB126303]; National Natural Science Foundation of China [30871938, 31072239]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ12, 2013FB13] ; National Major Basic Research Program [2009CB118704, 2010CB126303]; National Natural Science Foundation of China [30871938, 31072239]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ12, 2013FB13] ; National Major Basic Research Program [2009CB118704, 2010CB126303]; National Natural Science Foundation of China [30871938, 31072239]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ12, 2013FB13]
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Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19586
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorZhang, QY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China.
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Ke, Fei,He, Li-Bo,Zhang, Qi-Ya,et al. Nonstructural Protein NS80 Is Crucial in Recruiting Viral Components to Form Aquareoviral Factories[J]. PLOS ONE,2013,8(5):e63737.
APA Ke, Fei,He, Li-Bo,Zhang, Qi-Ya,&Zhang, QY .(2013).Nonstructural Protein NS80 Is Crucial in Recruiting Viral Components to Form Aquareoviral Factories.PLOS ONE,8(5),e63737.
MLA Ke, Fei,et al."Nonstructural Protein NS80 Is Crucial in Recruiting Viral Components to Form Aquareoviral Factories".PLOS ONE 8.5(2013):e63737.
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