IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Cloning and preliminary functional studies of the JAM-A gene in grass carp (Ctenopharyngodon idellus)
Du, Fukuan1,2; Su, Jianguo3; Huang, Rong1; Liao, Lanjie1; Zhu, Zuoyan1; Wang, Yaping1; Wang, YP (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 Donghu South Rd, Wuhan 430072, Peoples R China.
2013-06-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume34Issue:6Pages:1476-1484
AbstractGrass carp (Ctenopharyngodon idellus) is a very important aquaculture species in China and other South-East Asian countries; however, disease outbreaks in this species are frequent, resulting in huge economic losses. Grass carp hemorrhage caused by grass carp reovirus (GCRV) is one of the most serious diseases. Junction adhesion molecule A (JAM-A) is the mammalian receptor for reovirus, and has been well studied. However, the JAM-A gene in grass carp has not been studied so far. In this study, we cloned and elucidated the structure of the JAM-A gene in grass carp (GcJAM-A) and then studied its functions during grass carp hemorrhage. GcJAM-A is composed of 10 exons and 9 introns, and its full-length cDNA is 1833 bp long, with an 888 bp open reading frame (ORE) that encodes a 295 amino acid protein. The GcJAM-A protein is predicted to contain a typical transmembrane domain. Maternal expression pattern of GcJAM-A is observed during early embryogenesis, while zygote expression occurs at 8 h after hatching. GcJAM-A is expressed strongly in the gill, liver, intestine and kidney, while it is expressed poorly in the blood, brain, spleen and head kidney. Moreover, lower expression is observed in the gill, liver, intestine, brain, spleen and kidney of 30-month-old individuals, compared with 6-month-old. In a GcJAM-A-knockdown cell line (CIK) infected with GCRV, the expression of genes involved in the interferon and apoptosis pathways was significantly inhibited. These results suggest that GcJAM-A could be a receptor for GCRV. We have therefore managed to characterize the GcJAM-A gene and provide evidence for its role as a receptor for GCRV. Crown Copyright (C) 2013 Published by Elsevier Ltd. All rights reserved.; Grass carp (Ctenopharyngodon idellus) is a very important aquaculture species in China and other South-East Asian countries; however, disease outbreaks in this species are frequent, resulting in huge economic losses. Grass carp hemorrhage caused by grass carp reovirus (GCRV) is one of the most serious diseases. Junction adhesion molecule A (JAM-A) is the mammalian receptor for reovirus, and has been well studied. However, the JAM-A gene in grass carp has not been studied so far. In this study, we cloned and elucidated the structure of the JAM-A gene in grass carp (GcJAM-A) and then studied its functions during grass carp hemorrhage. GcJAM-A is composed of 10 exons and 9 introns, and its full-length cDNA is 1833 bp long, with an 888 bp open reading frame (ORE) that encodes a 295 amino acid protein. The GcJAM-A protein is predicted to contain a typical transmembrane domain. Maternal expression pattern of GcJAM-A is observed during early embryogenesis, while zygote expression occurs at 8 h after hatching. GcJAM-A is expressed strongly in the gill, liver, intestine and kidney, while it is expressed poorly in the blood, brain, spleen and head kidney. Moreover, lower expression is observed in the gill, liver, intestine, brain, spleen and kidney of 30-month-old individuals, compared with 6-month-old. In a GcJAM-A-knockdown cell line (CIK) infected with GCRV, the expression of genes involved in the interferon and apoptosis pathways was significantly inhibited. These results suggest that GcJAM-A could be a receptor for GCRV. We have therefore managed to characterize the GcJAM-A gene and provide evidence for its role as a receptor for GCRV. Crown Copyright (C) 2013 Published by Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordCtenopharyngodon Idellus Jam-a Grass Carp Hemorrhage
Department[Du, Fukuan ; Huang, Rong ; Liao, Lanjie ; Zhu, Zuoyan ; Wang, Yaping] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Du, Fukuan] Univ Chinese Acad Sci, Beijing 100039, Peoples R China ; [Su, Jianguo] Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China
DOI10.1016/j.fsi.2013.03.352
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [31101922]; '863' High Technology Project [2011AA100403] ; National Natural Science Foundation of China [31101922]; '863' High Technology Project [2011AA100403] ; National Natural Science Foundation of China [31101922]; '863' High Technology Project [2011AA100403] ; National Natural Science Foundation of China [31101922]; '863' High Technology Project [2011AA100403]
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000319646100011
WOS KeywordJUNCTIONAL ADHESION MOLECULE ; APOPTOSIS-INDUCING LIGAND ; INNATE IMMUNE-RESPONSE ; KINASE PKR PROMOTER ; REOVIRUS ATTACHMENT ; CRYSTAL-STRUCTURE ; INTERFERON-GAMMA ; VIRAL-INFECTIONS ; TUMOR-CELLS ; EXPRESSION
Funding OrganizationNational Natural Science Foundation of China [31101922]; '863' High Technology Project [2011AA100403] ; National Natural Science Foundation of China [31101922]; '863' High Technology Project [2011AA100403] ; National Natural Science Foundation of China [31101922]; '863' High Technology Project [2011AA100403] ; National Natural Science Foundation of China [31101922]; '863' High Technology Project [2011AA100403]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19585
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorWang, YP (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 Donghu South Rd, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
3.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China
Recommended Citation
GB/T 7714
Du, Fukuan,Su, Jianguo,Huang, Rong,et al. Cloning and preliminary functional studies of the JAM-A gene in grass carp (Ctenopharyngodon idellus)[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(6):1476-1484.
APA Du, Fukuan.,Su, Jianguo.,Huang, Rong.,Liao, Lanjie.,Zhu, Zuoyan.,...&Wang, YP .(2013).Cloning and preliminary functional studies of the JAM-A gene in grass carp (Ctenopharyngodon idellus).FISH & SHELLFISH IMMUNOLOGY,34(6),1476-1484.
MLA Du, Fukuan,et al."Cloning and preliminary functional studies of the JAM-A gene in grass carp (Ctenopharyngodon idellus)".FISH & SHELLFISH IMMUNOLOGY 34.6(2013):1476-1484.
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