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题名: Cloning and preliminary functional studies of the JAM-A gene in grass carp (Ctenopharyngodon idellus)
作者: Du, Fukuan1, 2; Su, Jianguo3; Huang, Rong1; Liao, Lanjie1; Zhu, Zuoyan1; Wang, Yaping1
通讯作者: Wang, YP (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 Donghu South Rd, Wuhan 430072, Peoples R China.
关键词: Ctenopharyngodon idellus ; JAM-A ; Grass carp hemorrhage
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2013-06-01
DOI: 10.1016/j.fsi.2013.03.352
卷: 34, 期:6, 页:1476-1484
收录类别: SCI
文章类型: Article
部门归属: [Du, Fukuan ; Huang, Rong ; Liao, Lanjie ; Zhu, Zuoyan ; Wang, Yaping] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Du, Fukuan] Univ Chinese Acad Sci, Beijing 100039, Peoples R China ; [Su, Jianguo] Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [31101922]; '863' High Technology Project [2011AA100403]
类目[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
摘要: Grass carp (Ctenopharyngodon idellus) is a very important aquaculture species in China and other South-East Asian countries; however, disease outbreaks in this species are frequent, resulting in huge economic losses. Grass carp hemorrhage caused by grass carp reovirus (GCRV) is one of the most serious diseases. Junction adhesion molecule A (JAM-A) is the mammalian receptor for reovirus, and has been well studied. However, the JAM-A gene in grass carp has not been studied so far. In this study, we cloned and elucidated the structure of the JAM-A gene in grass carp (GcJAM-A) and then studied its functions during grass carp hemorrhage. GcJAM-A is composed of 10 exons and 9 introns, and its full-length cDNA is 1833 bp long, with an 888 bp open reading frame (ORE) that encodes a 295 amino acid protein. The GcJAM-A protein is predicted to contain a typical transmembrane domain. Maternal expression pattern of GcJAM-A is observed during early embryogenesis, while zygote expression occurs at 8 h after hatching. GcJAM-A is expressed strongly in the gill, liver, intestine and kidney, while it is expressed poorly in the blood, brain, spleen and head kidney. Moreover, lower expression is observed in the gill, liver, intestine, brain, spleen and kidney of 30-month-old individuals, compared with 6-month-old. In a GcJAM-A-knockdown cell line (CIK) infected with GCRV, the expression of genes involved in the interferon and apoptosis pathways was significantly inhibited. These results suggest that GcJAM-A could be a receptor for GCRV. We have therefore managed to characterize the GcJAM-A gene and provide evidence for its role as a receptor for GCRV. Crown Copyright (C) 2013 Published by Elsevier Ltd. All rights reserved.
英文摘要: Grass carp (Ctenopharyngodon idellus) is a very important aquaculture species in China and other South-East Asian countries; however, disease outbreaks in this species are frequent, resulting in huge economic losses. Grass carp hemorrhage caused by grass carp reovirus (GCRV) is one of the most serious diseases. Junction adhesion molecule A (JAM-A) is the mammalian receptor for reovirus, and has been well studied. However, the JAM-A gene in grass carp has not been studied so far. In this study, we cloned and elucidated the structure of the JAM-A gene in grass carp (GcJAM-A) and then studied its functions during grass carp hemorrhage. GcJAM-A is composed of 10 exons and 9 introns, and its full-length cDNA is 1833 bp long, with an 888 bp open reading frame (ORE) that encodes a 295 amino acid protein. The GcJAM-A protein is predicted to contain a typical transmembrane domain. Maternal expression pattern of GcJAM-A is observed during early embryogenesis, while zygote expression occurs at 8 h after hatching. GcJAM-A is expressed strongly in the gill, liver, intestine and kidney, while it is expressed poorly in the blood, brain, spleen and head kidney. Moreover, lower expression is observed in the gill, liver, intestine, brain, spleen and kidney of 30-month-old individuals, compared with 6-month-old. In a GcJAM-A-knockdown cell line (CIK) infected with GCRV, the expression of genes involved in the interferon and apoptosis pathways was significantly inhibited. These results suggest that GcJAM-A could be a receptor for GCRV. We have therefore managed to characterize the GcJAM-A gene and provide evidence for its role as a receptor for GCRV. Crown Copyright (C) 2013 Published by Elsevier Ltd. All rights reserved.
关键词[WOS]: JUNCTIONAL ADHESION MOLECULE ; APOPTOSIS-INDUCING LIGAND ; INNATE IMMUNE-RESPONSE ; KINASE PKR PROMOTER ; REOVIRUS ATTACHMENT ; CRYSTAL-STRUCTURE ; INTERFERON-GAMMA ; VIRAL-INFECTIONS ; TUMOR-CELLS ; EXPRESSION
语种: 英语
WOS记录号: WOS:000319646100011
ISSN号: 1050-4648
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19585
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
3.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China

Recommended Citation:
Du, Fukuan; Su, Jianguo; Huang, Rong; Liao, Lanjie; Zhu, Zuoyan; Wang, Yaping.Cloning and preliminary functional studies of the JAM-A gene in grass carp (Ctenopharyngodon idellus),FISH & SHELLFISH IMMUNOLOGY,2013,34(6):1476-1484
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