| Functional characterization of a short peptidoglycan recognition protein, PGRP5 in grass carp Ctenopharyngodon idella | |
| Li, Jun Hua1,2; Chang, Ming Xian1; Xue, Na Na1,2; Nie, P.1; Chang, MX (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China. | |
| 2013-08-01 | |
| Source Publication | FISH & SHELLFISH IMMUNOLOGY
 |
| ISSN | 1050-4648 |
| Volume | 35Issue:2Pages:221-230 |
| Abstract | Peptidoglycan recognition proteins (PGRPs), which are evolutionarily conserved from insects to mammals, recognize bacterial peptidoglycan (PGN) and function in antibacterial innate immunity. In this study, a short-form PGRP, designated as gcPGRP5 was identified from grass carp Ctenopharyngodon idella. The deduced amino acid sequence of gcPGRP5 is composed of 180 residues with a conserved PGRP domain at the C-terminus. The gcPGRP5 gene consists of four exons and three introns, spacing approximately 2.3 kb in genomic sequence. Phylogenetic analysis demonstrated that the gcPGRP5 is clustered with other PGRP-S identified in teleost fish. The gcPGRP5 is constitutively expressed in all organs/tissues examined, and its expression was significantly induced in CIK cells treated with lipoteichoic acid (LTA), polyinosinic polycytidylic acid (Poly I:C) and PGN. Fluorescence analysis showed that gcPGRP5 is distributed in cytoplasm of CIK cells, and cell lysates from CIK cells transfected with pTurbo-gcPGRP5-GFP and ptGFP1-gcPGRP5 plasmids display the binding activity and peptidoglycan-lytic amidase activity toward Lys-PGN from Staphylococcus aureus and Dap-PGN from Bacillus subtilis. Furthermore, heat-shock protein70 (Hsp70), and MyD88, an adaptor molecule in Toll-like receptor pathway, had an increased expression in CIK cells overexpressed with gcPGRP5. It is thus indicated that gcPGRP5 exhibits amidase activity, and also possesses roles in anti-stress, and in Toll-like receptor signaling pathway. (C) 2013 Elsevier Ltd. All rights reserved.; Peptidoglycan recognition proteins (PGRPs), which are evolutionarily conserved from insects to mammals, recognize bacterial peptidoglycan (PGN) and function in antibacterial innate immunity. In this study, a short-form PGRP, designated as gcPGRP5 was identified from grass carp Ctenopharyngodon idella. The deduced amino acid sequence of gcPGRP5 is composed of 180 residues with a conserved PGRP domain at the C-terminus. The gcPGRP5 gene consists of four exons and three introns, spacing approximately 2.3 kb in genomic sequence. Phylogenetic analysis demonstrated that the gcPGRP5 is clustered with other PGRP-S identified in teleost fish. The gcPGRP5 is constitutively expressed in all organs/tissues examined, and its expression was significantly induced in CIK cells treated with lipoteichoic acid (LTA), polyinosinic polycytidylic acid (Poly I:C) and PGN. Fluorescence analysis showed that gcPGRP5 is distributed in cytoplasm of CIK cells, and cell lysates from CIK cells transfected with pTurbo-gcPGRP5-GFP and ptGFP1-gcPGRP5 plasmids display the binding activity and peptidoglycan-lytic amidase activity toward Lys-PGN from Staphylococcus aureus and Dap-PGN from Bacillus subtilis. Furthermore, heat-shock protein70 (Hsp70), and MyD88, an adaptor molecule in Toll-like receptor pathway, had an increased expression in CIK cells overexpressed with gcPGRP5. It is thus indicated that gcPGRP5 exhibits amidase activity, and also possesses roles in anti-stress, and in Toll-like receptor signaling pathway. (C) 2013 Elsevier Ltd. All rights reserved. |
| Subtype | Article |
| Keyword | Peptidoglycan Recognition Protein Pgrp5 Peptidoglycan-binding Activity Amidase Activity Grass Carp |
| Department | [Li, Jun Hua ; Chang, Ming Xian ; Xue, Na Na ; Nie, P.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China ; [Li, Jun Hua ; Xue, Na Na] Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| DOI | 10.1016/j.fsi.2013.04.025 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | National Natural Science Foundation of China [31172408]; National Basic Research Programme (973 Programme) of China [2009CB118703] ; National Natural Science Foundation of China [31172408]; National Basic Research Programme (973 Programme) of China [2009CB118703] ; National Natural Science Foundation of China [31172408]; National Basic Research Programme (973 Programme) of China [2009CB118703] ; National Natural Science Foundation of China [31172408]; National Basic Research Programme (973 Programme) of China [2009CB118703] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS Subject | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS ID | WOS:000322207500004 |
| WOS Keyword | L-ALANINE AMIDASE ; INNATE IMMUNITY ; POSITIVE BACTERIA ; ANTIBACTERIAL ACTIVITY ; MOLECULAR-CLONING ; DROSOPHILA ; EXPRESSION ; TOLL ; ZEBRAFISH ; RECEPTORS |
| Funding Organization | National Natural Science Foundation of China [31172408]; National Basic Research Programme (973 Programme) of China [2009CB118703] ; National Natural Science Foundation of China [31172408]; National Basic Research Programme (973 Programme) of China [2009CB118703] ; National Natural Science Foundation of China [31172408]; National Basic Research Programme (973 Programme) of China [2009CB118703] ; National Natural Science Foundation of China [31172408]; National Basic Research Programme (973 Programme) of China [2009CB118703] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/19581 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Corresponding Author | Chang, MX (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China. |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| Recommended Citation GB/T 7714 | Li, Jun Hua,Chang, Ming Xian,Xue, Na Na,et al. Functional characterization of a short peptidoglycan recognition protein, PGRP5 in grass carp Ctenopharyngodon idella[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(2):221-230. |
| APA | Li, Jun Hua,Chang, Ming Xian,Xue, Na Na,Nie, P.,&Chang, MX .(2013).Functional characterization of a short peptidoglycan recognition protein, PGRP5 in grass carp Ctenopharyngodon idella.FISH & SHELLFISH IMMUNOLOGY,35(2),221-230. |
| MLA | Li, Jun Hua,et al."Functional characterization of a short peptidoglycan recognition protein, PGRP5 in grass carp Ctenopharyngodon idella".FISH & SHELLFISH IMMUNOLOGY 35.2(2013):221-230. |
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