| Microsatellite-centromere mapping in bighead carp (Aristichthys nobilis) using gynogenetic diploid families | |
| Liu, Lusha1,2; Tong, Jingou1; Guo, Wenjie1,2; Yu, Xiaomu1; Tong, JO (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China. | |
| 2013-08-01 | |
| Source Publication | AQUACULTURE RESEARCH
 |
| ISSN | 1355-557X |
| Volume | 44Issue:9Pages:1470-1488 |
| Abstract | Gene-centromere mapping using half-tetrad analysis is a powerful tool for understanding chromosomal behaviour and determining the position of centromeres in relation to genes or markers in fish. In this study, eight gynogenetic diploid families induced by inhibiting of the second meiotic division were genotyped at 66 microsatellite loci for microsatellite-centromere (M-C) mapping in bighead carp (Aristichthys nobilis). The absence of paternal alleles verified the success of gynogenetic development in all gynogenetic families. All loci were consistent with Mendelian segregation in control families. The estimated recombination frequency (y) ranged from 0.057 to 0.875 with an average of 0.477 +/- 0.222. Seventeen loci (25.76%) showed high M-C recombination frequencies of over 0.667. M-C distances ranged from 2.85 to 43.75cM under the assumption of complete interference. Thus, these loci are distributed from the centromeres to the telomeres of their respective chromosomes, while mainly in the intermediate region. Information on centromere mapping could serve as a starting point to consolidate the genetic linkage groups in bighead carp.; Gene-centromere mapping using half-tetrad analysis is a powerful tool for understanding chromosomal behaviour and determining the position of centromeres in relation to genes or markers in fish. In this study, eight gynogenetic diploid families induced by inhibiting of the second meiotic division were genotyped at 66 microsatellite loci for microsatellite-centromere (M-C) mapping in bighead carp (Aristichthys nobilis). The absence of paternal alleles verified the success of gynogenetic development in all gynogenetic families. All loci were consistent with Mendelian segregation in control families. The estimated recombination frequency (y) ranged from 0.057 to 0.875 with an average of 0.477 +/- 0.222. Seventeen loci (25.76%) showed high M-C recombination frequencies of over 0.667. M-C distances ranged from 2.85 to 43.75cM under the assumption of complete interference. Thus, these loci are distributed from the centromeres to the telomeres of their respective chromosomes, while mainly in the intermediate region. Information on centromere mapping could serve as a starting point to consolidate the genetic linkage groups in bighead carp. |
| Subtype | Article |
| Keyword | Gynogenesis Half-tetrad Analysis Microsatellites Gene-centromere Mapping Bighead Carp (Aristichthys Nobilis) |
| Department | [Liu, Lusha ; Tong, Jingou ; Guo, Wenjie ; Yu, Xiaomu] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Liu, Lusha ; Guo, Wenjie] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| DOI | 10.1111/j.1365-2109.2012.03155.x |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | Ministry of Agriculture [200903045, 2011-G12]; Ministry of Science and Technology of China [2010CB126305]; CAS studentship ; Ministry of Agriculture [200903045, 2011-G12]; Ministry of Science and Technology of China [2010CB126305]; CAS studentship ; Ministry of Agriculture [200903045, 2011-G12]; Ministry of Science and Technology of China [2010CB126305]; CAS studentship ; Ministry of Agriculture [200903045, 2011-G12]; Ministry of Science and Technology of China [2010CB126305]; CAS studentship |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Fisheries |
| WOS Subject | Fisheries |
| WOS ID | WOS:000322814000014 |
| WOS Keyword | HALF-TETRAD ANALYSIS ; CROSS-SPECIES APPLICATION ; ZEBRAFISH DANIO-RERIO ; HYPOPHTHALMICHTHYS-MOLITRIX ; RAINBOW-TROUT ; SEGREGATION DISTORTION ; ORYZIAS-LATIPES ; LINKAGE MAP ; DNA MARKERS ; LOCI |
| Funding Organization | Ministry of Agriculture [200903045, 2011-G12]; Ministry of Science and Technology of China [2010CB126305]; CAS studentship ; Ministry of Agriculture [200903045, 2011-G12]; Ministry of Science and Technology of China [2010CB126305]; CAS studentship ; Ministry of Agriculture [200903045, 2011-G12]; Ministry of Science and Technology of China [2010CB126305]; CAS studentship ; Ministry of Agriculture [200903045, 2011-G12]; Ministry of Science and Technology of China [2010CB126305]; CAS studentship |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/19580 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Corresponding Author | Tong, JO (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China. |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| Recommended Citation GB/T 7714 | Liu, Lusha,Tong, Jingou,Guo, Wenjie,et al. Microsatellite-centromere mapping in bighead carp (Aristichthys nobilis) using gynogenetic diploid families[J]. AQUACULTURE RESEARCH,2013,44(9):1470-1488. |
| APA | Liu, Lusha,Tong, Jingou,Guo, Wenjie,Yu, Xiaomu,&Tong, JO .(2013).Microsatellite-centromere mapping in bighead carp (Aristichthys nobilis) using gynogenetic diploid families.AQUACULTURE RESEARCH,44(9),1470-1488. |
| MLA | Liu, Lusha,et al."Microsatellite-centromere mapping in bighead carp (Aristichthys nobilis) using gynogenetic diploid families".AQUACULTURE RESEARCH 44.9(2013):1470-1488. |
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