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题名: Microsatellite-centromere mapping in bighead carp (Aristichthys nobilis) using gynogenetic diploid families
作者: Liu, Lusha1, 2; Tong, Jingou1; Guo, Wenjie1, 2; Yu, Xiaomu1
通讯作者: Tong, JO (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
关键词: gynogenesis ; half-tetrad analysis ; microsatellites ; gene-centromere mapping ; bighead carp (Aristichthys nobilis)
刊名: AQUACULTURE RESEARCH
发表日期: 2013-08-01
DOI: 10.1111/j.1365-2109.2012.03155.x
卷: 44, 期:9, 页:1470-1488
收录类别: SCI
文章类型: Article
部门归属: [Liu, Lusha ; Tong, Jingou ; Guo, Wenjie ; Yu, Xiaomu] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Liu, Lusha ; Guo, Wenjie] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: Ministry of Agriculture [200903045, 2011-G12]; Ministry of Science and Technology of China [2010CB126305]; CAS studentship
类目[WOS]: Fisheries
研究领域[WOS]: Fisheries
摘要: Gene-centromere mapping using half-tetrad analysis is a powerful tool for understanding chromosomal behaviour and determining the position of centromeres in relation to genes or markers in fish. In this study, eight gynogenetic diploid families induced by inhibiting of the second meiotic division were genotyped at 66 microsatellite loci for microsatellite-centromere (M-C) mapping in bighead carp (Aristichthys nobilis). The absence of paternal alleles verified the success of gynogenetic development in all gynogenetic families. All loci were consistent with Mendelian segregation in control families. The estimated recombination frequency (y) ranged from 0.057 to 0.875 with an average of 0.477 +/- 0.222. Seventeen loci (25.76%) showed high M-C recombination frequencies of over 0.667. M-C distances ranged from 2.85 to 43.75cM under the assumption of complete interference. Thus, these loci are distributed from the centromeres to the telomeres of their respective chromosomes, while mainly in the intermediate region. Information on centromere mapping could serve as a starting point to consolidate the genetic linkage groups in bighead carp.
英文摘要: Gene-centromere mapping using half-tetrad analysis is a powerful tool for understanding chromosomal behaviour and determining the position of centromeres in relation to genes or markers in fish. In this study, eight gynogenetic diploid families induced by inhibiting of the second meiotic division were genotyped at 66 microsatellite loci for microsatellite-centromere (M-C) mapping in bighead carp (Aristichthys nobilis). The absence of paternal alleles verified the success of gynogenetic development in all gynogenetic families. All loci were consistent with Mendelian segregation in control families. The estimated recombination frequency (y) ranged from 0.057 to 0.875 with an average of 0.477 +/- 0.222. Seventeen loci (25.76%) showed high M-C recombination frequencies of over 0.667. M-C distances ranged from 2.85 to 43.75cM under the assumption of complete interference. Thus, these loci are distributed from the centromeres to the telomeres of their respective chromosomes, while mainly in the intermediate region. Information on centromere mapping could serve as a starting point to consolidate the genetic linkage groups in bighead carp.
关键词[WOS]: HALF-TETRAD ANALYSIS ; CROSS-SPECIES APPLICATION ; ZEBRAFISH DANIO-RERIO ; HYPOPHTHALMICHTHYS-MOLITRIX ; RAINBOW-TROUT ; SEGREGATION DISTORTION ; ORYZIAS-LATIPES ; LINKAGE MAP ; DNA MARKERS ; LOCI
语种: 英语
WOS记录号: WOS:000322814000014
ISSN号: 1355-557X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19580
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China

Recommended Citation:
Liu, Lusha; Tong, Jingou; Guo, Wenjie; Yu, Xiaomu.Microsatellite-centromere mapping in bighead carp (Aristichthys nobilis) using gynogenetic diploid families,AQUACULTURE RESEARCH,2013,44(9):1470-1488
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