IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Microsatellite-centromere mapping in bighead carp (Aristichthys nobilis) using gynogenetic diploid families
Liu, Lusha1,2; Tong, Jingou1; Guo, Wenjie1,2; Yu, Xiaomu1; Tong, JO (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
2013-08-01
Source PublicationAQUACULTURE RESEARCH
ISSN1355-557X
Volume44Issue:9Pages:1470-1488
AbstractGene-centromere mapping using half-tetrad analysis is a powerful tool for understanding chromosomal behaviour and determining the position of centromeres in relation to genes or markers in fish. In this study, eight gynogenetic diploid families induced by inhibiting of the second meiotic division were genotyped at 66 microsatellite loci for microsatellite-centromere (M-C) mapping in bighead carp (Aristichthys nobilis). The absence of paternal alleles verified the success of gynogenetic development in all gynogenetic families. All loci were consistent with Mendelian segregation in control families. The estimated recombination frequency (y) ranged from 0.057 to 0.875 with an average of 0.477 +/- 0.222. Seventeen loci (25.76%) showed high M-C recombination frequencies of over 0.667. M-C distances ranged from 2.85 to 43.75cM under the assumption of complete interference. Thus, these loci are distributed from the centromeres to the telomeres of their respective chromosomes, while mainly in the intermediate region. Information on centromere mapping could serve as a starting point to consolidate the genetic linkage groups in bighead carp.; Gene-centromere mapping using half-tetrad analysis is a powerful tool for understanding chromosomal behaviour and determining the position of centromeres in relation to genes or markers in fish. In this study, eight gynogenetic diploid families induced by inhibiting of the second meiotic division were genotyped at 66 microsatellite loci for microsatellite-centromere (M-C) mapping in bighead carp (Aristichthys nobilis). The absence of paternal alleles verified the success of gynogenetic development in all gynogenetic families. All loci were consistent with Mendelian segregation in control families. The estimated recombination frequency (y) ranged from 0.057 to 0.875 with an average of 0.477 +/- 0.222. Seventeen loci (25.76%) showed high M-C recombination frequencies of over 0.667. M-C distances ranged from 2.85 to 43.75cM under the assumption of complete interference. Thus, these loci are distributed from the centromeres to the telomeres of their respective chromosomes, while mainly in the intermediate region. Information on centromere mapping could serve as a starting point to consolidate the genetic linkage groups in bighead carp.
SubtypeArticle
KeywordGynogenesis Half-tetrad Analysis Microsatellites Gene-centromere Mapping Bighead Carp (Aristichthys Nobilis)
Department[Liu, Lusha ; Tong, Jingou ; Guo, Wenjie ; Yu, Xiaomu] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Liu, Lusha ; Guo, Wenjie] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
DOI10.1111/j.1365-2109.2012.03155.x
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationMinistry of Agriculture [200903045, 2011-G12]; Ministry of Science and Technology of China [2010CB126305]; CAS studentship ; Ministry of Agriculture [200903045, 2011-G12]; Ministry of Science and Technology of China [2010CB126305]; CAS studentship ; Ministry of Agriculture [200903045, 2011-G12]; Ministry of Science and Technology of China [2010CB126305]; CAS studentship ; Ministry of Agriculture [200903045, 2011-G12]; Ministry of Science and Technology of China [2010CB126305]; CAS studentship
Indexed BySCI
Language英语
WOS Research AreaFisheries
WOS SubjectFisheries
WOS IDWOS:000322814000014
WOS KeywordHALF-TETRAD ANALYSIS ; CROSS-SPECIES APPLICATION ; ZEBRAFISH DANIO-RERIO ; HYPOPHTHALMICHTHYS-MOLITRIX ; RAINBOW-TROUT ; SEGREGATION DISTORTION ; ORYZIAS-LATIPES ; LINKAGE MAP ; DNA MARKERS ; LOCI
Funding OrganizationMinistry of Agriculture [200903045, 2011-G12]; Ministry of Science and Technology of China [2010CB126305]; CAS studentship ; Ministry of Agriculture [200903045, 2011-G12]; Ministry of Science and Technology of China [2010CB126305]; CAS studentship ; Ministry of Agriculture [200903045, 2011-G12]; Ministry of Science and Technology of China [2010CB126305]; CAS studentship ; Ministry of Agriculture [200903045, 2011-G12]; Ministry of Science and Technology of China [2010CB126305]; CAS studentship
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19580
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorTong, JO (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Liu, Lusha,Tong, Jingou,Guo, Wenjie,et al. Microsatellite-centromere mapping in bighead carp (Aristichthys nobilis) using gynogenetic diploid families[J]. AQUACULTURE RESEARCH,2013,44(9):1470-1488.
APA Liu, Lusha,Tong, Jingou,Guo, Wenjie,Yu, Xiaomu,&Tong, JO .(2013).Microsatellite-centromere mapping in bighead carp (Aristichthys nobilis) using gynogenetic diploid families.AQUACULTURE RESEARCH,44(9),1470-1488.
MLA Liu, Lusha,et al."Microsatellite-centromere mapping in bighead carp (Aristichthys nobilis) using gynogenetic diploid families".AQUACULTURE RESEARCH 44.9(2013):1470-1488.
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