| Intestinal microbiota of gibel carp (Carassius auratus gibelio) and its origin as revealed by 454 pyrosequencing | |
| Wu, Shan-Gong1; Tian, Jing-Yun2; Gatesoupe, Francois-Joel3; Li, Wen-Xiang1; Zou, Hong1; Yang, Bao-Juan1; Wang, Gui-Tang1; Wang, GT (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China. | |
| 2013-09-01 | |
| Source Publication | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
 |
| ISSN | 0959-3993 |
| Volume | 29Issue:9Pages:1585-1595 |
| Abstract | The intestinal microbiota has received increasing attention, as it influences growth, feed conversion, epithelial development, immunity as well as the intrusion of pathogenic microorganisms in the intestinal tract. In this study, pyrosequencing was used to explore the bacterial community of the intestine in gibel carp (Carassius auratus gibelio), and the origin of these microorganisms. The results disclosed great bacterial diversities in the carp intestines and cultured environments. The gibel carp harbored characteristic intestinal microbiota, where Proteobacteria were predominant, followed by Firmicutes. The analysis on the 10 most abundant bacterial operational taxonomic units (OTUs) revealed a majority of Firmicutes in the intestinal content (by decreasing order: Veilonella sp., Lachnospiraceae, Lactobacillales, Streptococcus sp., and Lactobacillus sp.). The second most abundant OTU was Rothia sp. (Actinobacteria). The most likely potential probiotics (Lactobacillus sp., and Bacillus sp.) and opportunists (Aeromonas sp., and Acinetobacter sp.) were not much abundant. Bacterial community comparisons showed that the intestinal community was closely related to that of the sediment, indicating the importance of sediment as source of gut bacteria in gibel carp. However, 37.95 % of the OTUs detected in feed were retrieved in the intestine, suggesting that food may influence markedly the microbiota of gibel carp, and therefore may be exploited for oral administration of probiotics.; The intestinal microbiota has received increasing attention, as it influences growth, feed conversion, epithelial development, immunity as well as the intrusion of pathogenic microorganisms in the intestinal tract. In this study, pyrosequencing was used to explore the bacterial community of the intestine in gibel carp (Carassius auratus gibelio), and the origin of these microorganisms. The results disclosed great bacterial diversities in the carp intestines and cultured environments. The gibel carp harbored characteristic intestinal microbiota, where Proteobacteria were predominant, followed by Firmicutes. The analysis on the 10 most abundant bacterial operational taxonomic units (OTUs) revealed a majority of Firmicutes in the intestinal content (by decreasing order: Veilonella sp., Lachnospiraceae, Lactobacillales, Streptococcus sp., and Lactobacillus sp.). The second most abundant OTU was Rothia sp. (Actinobacteria). The most likely potential probiotics (Lactobacillus sp., and Bacillus sp.) and opportunists (Aeromonas sp., and Acinetobacter sp.) were not much abundant. Bacterial community comparisons showed that the intestinal community was closely related to that of the sediment, indicating the importance of sediment as source of gut bacteria in gibel carp. However, 37.95 % of the OTUs detected in feed were retrieved in the intestine, suggesting that food may influence markedly the microbiota of gibel carp, and therefore may be exploited for oral administration of probiotics. |
| Subtype | Article |
| Keyword | Gibel Carp Intestine Microbiota Pyrosequencing |
| Department | [Wu, Shan-Gong ; Li, Wen-Xiang ; Zou, Hong ; Yang, Bao-Juan ; Wang, Gui-Tang] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China ; [Tian, Jing-Yun] Natl Oceanog Ctr, Qingdao 266071, Shandong, Peoples R China ; [Gatesoupe, Francois-Joel] IFREMER, Ctr Brest, INRA, UR Nutr Metab & Aquaculture 1067, F-29280 Plouzane, France |
| DOI | 10.1007/s11274-013-1322-4 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | National Natural Science Foundation of China [31272706]; National Basic Research Program of China [2009CB118705]; Natural Science Foundation of Hubei Province [2009CDB331]; earmarked fund for China Agriculture Research System [CARS-46-08] ; National Natural Science Foundation of China [31272706]; National Basic Research Program of China [2009CB118705]; Natural Science Foundation of Hubei Province [2009CDB331]; earmarked fund for China Agriculture Research System [CARS-46-08] ; National Natural Science Foundation of China [31272706]; National Basic Research Program of China [2009CB118705]; Natural Science Foundation of Hubei Province [2009CDB331]; earmarked fund for China Agriculture Research System [CARS-46-08] ; National Natural Science Foundation of China [31272706]; National Basic Research Program of China [2009CB118705]; Natural Science Foundation of Hubei Province [2009CDB331]; earmarked fund for China Agriculture Research System [CARS-46-08] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Biotechnology & Applied Microbiology |
| WOS Subject | Biotechnology & Applied Microbiology |
| WOS ID | WOS:000323223300005 |
| WOS Keyword | BACTERIAL-POPULATIONS ; GUT MICROBIOTA ; DIVERSITY ; IDENTIFICATION ; FISH ; AMPLIFICATION ; MICROFLORA ; LARVAE ; MUCUS ; DNA |
| Funding Organization | National Natural Science Foundation of China [31272706]; National Basic Research Program of China [2009CB118705]; Natural Science Foundation of Hubei Province [2009CDB331]; earmarked fund for China Agriculture Research System [CARS-46-08] ; National Natural Science Foundation of China [31272706]; National Basic Research Program of China [2009CB118705]; Natural Science Foundation of Hubei Province [2009CDB331]; earmarked fund for China Agriculture Research System [CARS-46-08] ; National Natural Science Foundation of China [31272706]; National Basic Research Program of China [2009CB118705]; Natural Science Foundation of Hubei Province [2009CDB331]; earmarked fund for China Agriculture Research System [CARS-46-08] ; National Natural Science Foundation of China [31272706]; National Basic Research Program of China [2009CB118705]; Natural Science Foundation of Hubei Province [2009CDB331]; earmarked fund for China Agriculture Research System [CARS-46-08] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/19579 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Corresponding Author | Wang, GT (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China. |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China 2.Natl Oceanog Ctr, Qingdao 266071, Shandong, Peoples R China 3.IFREMER, Ctr Brest, INRA, UR Nutr Metab & Aquaculture 1067, F-29280 Plouzane, France |
| Recommended Citation GB/T 7714 | Wu, Shan-Gong,Tian, Jing-Yun,Gatesoupe, Francois-Joel,et al. Intestinal microbiota of gibel carp (Carassius auratus gibelio) and its origin as revealed by 454 pyrosequencing[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2013,29(9):1585-1595. |
| APA | Wu, Shan-Gong.,Tian, Jing-Yun.,Gatesoupe, Francois-Joel.,Li, Wen-Xiang.,Zou, Hong.,...&Wang, GT .(2013).Intestinal microbiota of gibel carp (Carassius auratus gibelio) and its origin as revealed by 454 pyrosequencing.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,29(9),1585-1595. |
| MLA | Wu, Shan-Gong,et al."Intestinal microbiota of gibel carp (Carassius auratus gibelio) and its origin as revealed by 454 pyrosequencing".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 29.9(2013):1585-1595. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Intestinal microbiot（588KB） | 开放获取 | CC BY-NC-SA | View Application Full Text | |||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment