IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Intestinal microbiota of gibel carp (Carassius auratus gibelio) and its origin as revealed by 454 pyrosequencing
Wu, Shan-Gong1; Tian, Jing-Yun2; Gatesoupe, Francois-Joel3; Li, Wen-Xiang1; Zou, Hong1; Yang, Bao-Juan1; Wang, Gui-Tang1; Wang, GT (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China.
2013-09-01
Source PublicationWORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
ISSN0959-3993
Volume29Issue:9Pages:1585-1595
AbstractThe intestinal microbiota has received increasing attention, as it influences growth, feed conversion, epithelial development, immunity as well as the intrusion of pathogenic microorganisms in the intestinal tract. In this study, pyrosequencing was used to explore the bacterial community of the intestine in gibel carp (Carassius auratus gibelio), and the origin of these microorganisms. The results disclosed great bacterial diversities in the carp intestines and cultured environments. The gibel carp harbored characteristic intestinal microbiota, where Proteobacteria were predominant, followed by Firmicutes. The analysis on the 10 most abundant bacterial operational taxonomic units (OTUs) revealed a majority of Firmicutes in the intestinal content (by decreasing order: Veilonella sp., Lachnospiraceae, Lactobacillales, Streptococcus sp., and Lactobacillus sp.). The second most abundant OTU was Rothia sp. (Actinobacteria). The most likely potential probiotics (Lactobacillus sp., and Bacillus sp.) and opportunists (Aeromonas sp., and Acinetobacter sp.) were not much abundant. Bacterial community comparisons showed that the intestinal community was closely related to that of the sediment, indicating the importance of sediment as source of gut bacteria in gibel carp. However, 37.95 % of the OTUs detected in feed were retrieved in the intestine, suggesting that food may influence markedly the microbiota of gibel carp, and therefore may be exploited for oral administration of probiotics.; The intestinal microbiota has received increasing attention, as it influences growth, feed conversion, epithelial development, immunity as well as the intrusion of pathogenic microorganisms in the intestinal tract. In this study, pyrosequencing was used to explore the bacterial community of the intestine in gibel carp (Carassius auratus gibelio), and the origin of these microorganisms. The results disclosed great bacterial diversities in the carp intestines and cultured environments. The gibel carp harbored characteristic intestinal microbiota, where Proteobacteria were predominant, followed by Firmicutes. The analysis on the 10 most abundant bacterial operational taxonomic units (OTUs) revealed a majority of Firmicutes in the intestinal content (by decreasing order: Veilonella sp., Lachnospiraceae, Lactobacillales, Streptococcus sp., and Lactobacillus sp.). The second most abundant OTU was Rothia sp. (Actinobacteria). The most likely potential probiotics (Lactobacillus sp., and Bacillus sp.) and opportunists (Aeromonas sp., and Acinetobacter sp.) were not much abundant. Bacterial community comparisons showed that the intestinal community was closely related to that of the sediment, indicating the importance of sediment as source of gut bacteria in gibel carp. However, 37.95 % of the OTUs detected in feed were retrieved in the intestine, suggesting that food may influence markedly the microbiota of gibel carp, and therefore may be exploited for oral administration of probiotics.
SubtypeArticle
KeywordGibel Carp Intestine Microbiota Pyrosequencing
Department[Wu, Shan-Gong ; Li, Wen-Xiang ; Zou, Hong ; Yang, Bao-Juan ; Wang, Gui-Tang] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China ; [Tian, Jing-Yun] Natl Oceanog Ctr, Qingdao 266071, Shandong, Peoples R China ; [Gatesoupe, Francois-Joel] IFREMER, Ctr Brest, INRA, UR Nutr Metab & Aquaculture 1067, F-29280 Plouzane, France
DOI10.1007/s11274-013-1322-4
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [31272706]; National Basic Research Program of China [2009CB118705]; Natural Science Foundation of Hubei Province [2009CDB331]; earmarked fund for China Agriculture Research System [CARS-46-08] ; National Natural Science Foundation of China [31272706]; National Basic Research Program of China [2009CB118705]; Natural Science Foundation of Hubei Province [2009CDB331]; earmarked fund for China Agriculture Research System [CARS-46-08] ; National Natural Science Foundation of China [31272706]; National Basic Research Program of China [2009CB118705]; Natural Science Foundation of Hubei Province [2009CDB331]; earmarked fund for China Agriculture Research System [CARS-46-08] ; National Natural Science Foundation of China [31272706]; National Basic Research Program of China [2009CB118705]; Natural Science Foundation of Hubei Province [2009CDB331]; earmarked fund for China Agriculture Research System [CARS-46-08]
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000323223300005
WOS KeywordBACTERIAL-POPULATIONS ; GUT MICROBIOTA ; DIVERSITY ; IDENTIFICATION ; FISH ; AMPLIFICATION ; MICROFLORA ; LARVAE ; MUCUS ; DNA
Funding OrganizationNational Natural Science Foundation of China [31272706]; National Basic Research Program of China [2009CB118705]; Natural Science Foundation of Hubei Province [2009CDB331]; earmarked fund for China Agriculture Research System [CARS-46-08] ; National Natural Science Foundation of China [31272706]; National Basic Research Program of China [2009CB118705]; Natural Science Foundation of Hubei Province [2009CDB331]; earmarked fund for China Agriculture Research System [CARS-46-08] ; National Natural Science Foundation of China [31272706]; National Basic Research Program of China [2009CB118705]; Natural Science Foundation of Hubei Province [2009CDB331]; earmarked fund for China Agriculture Research System [CARS-46-08] ; National Natural Science Foundation of China [31272706]; National Basic Research Program of China [2009CB118705]; Natural Science Foundation of Hubei Province [2009CDB331]; earmarked fund for China Agriculture Research System [CARS-46-08]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19579
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorWang, GT (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
2.Natl Oceanog Ctr, Qingdao 266071, Shandong, Peoples R China
3.IFREMER, Ctr Brest, INRA, UR Nutr Metab & Aquaculture 1067, F-29280 Plouzane, France
Recommended Citation
GB/T 7714
Wu, Shan-Gong,Tian, Jing-Yun,Gatesoupe, Francois-Joel,et al. Intestinal microbiota of gibel carp (Carassius auratus gibelio) and its origin as revealed by 454 pyrosequencing[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2013,29(9):1585-1595.
APA Wu, Shan-Gong.,Tian, Jing-Yun.,Gatesoupe, Francois-Joel.,Li, Wen-Xiang.,Zou, Hong.,...&Wang, GT .(2013).Intestinal microbiota of gibel carp (Carassius auratus gibelio) and its origin as revealed by 454 pyrosequencing.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,29(9),1585-1595.
MLA Wu, Shan-Gong,et al."Intestinal microbiota of gibel carp (Carassius auratus gibelio) and its origin as revealed by 454 pyrosequencing".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 29.9(2013):1585-1595.
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