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题名: Intestinal microbiota of gibel carp (Carassius auratus gibelio) and its origin as revealed by 454 pyrosequencing
作者: Wu, Shan-Gong1; Tian, Jing-Yun2; Gatesoupe, Francois-Joel3; Li, Wen-Xiang1; Zou, Hong1; Yang, Bao-Juan1; Wang, Gui-Tang1
通讯作者: Wang, GT (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China.
关键词: Gibel carp ; Intestine ; Microbiota ; Pyrosequencing
刊名: WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
发表日期: 2013-09-01
DOI: 10.1007/s11274-013-1322-4
卷: 29, 期:9, 页:1585-1595
收录类别: SCI
文章类型: Article
部门归属: [Wu, Shan-Gong ; Li, Wen-Xiang ; Zou, Hong ; Yang, Bao-Juan ; Wang, Gui-Tang] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China ; [Tian, Jing-Yun] Natl Oceanog Ctr, Qingdao 266071, Shandong, Peoples R China ; [Gatesoupe, Francois-Joel] IFREMER, Ctr Brest, INRA, UR Nutr Metab & Aquaculture 1067, F-29280 Plouzane, France
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [31272706]; National Basic Research Program of China [2009CB118705]; Natural Science Foundation of Hubei Province [2009CDB331]; earmarked fund for China Agriculture Research System [CARS-46-08]
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
摘要: The intestinal microbiota has received increasing attention, as it influences growth, feed conversion, epithelial development, immunity as well as the intrusion of pathogenic microorganisms in the intestinal tract. In this study, pyrosequencing was used to explore the bacterial community of the intestine in gibel carp (Carassius auratus gibelio), and the origin of these microorganisms. The results disclosed great bacterial diversities in the carp intestines and cultured environments. The gibel carp harbored characteristic intestinal microbiota, where Proteobacteria were predominant, followed by Firmicutes. The analysis on the 10 most abundant bacterial operational taxonomic units (OTUs) revealed a majority of Firmicutes in the intestinal content (by decreasing order: Veilonella sp., Lachnospiraceae, Lactobacillales, Streptococcus sp., and Lactobacillus sp.). The second most abundant OTU was Rothia sp. (Actinobacteria). The most likely potential probiotics (Lactobacillus sp., and Bacillus sp.) and opportunists (Aeromonas sp., and Acinetobacter sp.) were not much abundant. Bacterial community comparisons showed that the intestinal community was closely related to that of the sediment, indicating the importance of sediment as source of gut bacteria in gibel carp. However, 37.95 % of the OTUs detected in feed were retrieved in the intestine, suggesting that food may influence markedly the microbiota of gibel carp, and therefore may be exploited for oral administration of probiotics.
英文摘要: The intestinal microbiota has received increasing attention, as it influences growth, feed conversion, epithelial development, immunity as well as the intrusion of pathogenic microorganisms in the intestinal tract. In this study, pyrosequencing was used to explore the bacterial community of the intestine in gibel carp (Carassius auratus gibelio), and the origin of these microorganisms. The results disclosed great bacterial diversities in the carp intestines and cultured environments. The gibel carp harbored characteristic intestinal microbiota, where Proteobacteria were predominant, followed by Firmicutes. The analysis on the 10 most abundant bacterial operational taxonomic units (OTUs) revealed a majority of Firmicutes in the intestinal content (by decreasing order: Veilonella sp., Lachnospiraceae, Lactobacillales, Streptococcus sp., and Lactobacillus sp.). The second most abundant OTU was Rothia sp. (Actinobacteria). The most likely potential probiotics (Lactobacillus sp., and Bacillus sp.) and opportunists (Aeromonas sp., and Acinetobacter sp.) were not much abundant. Bacterial community comparisons showed that the intestinal community was closely related to that of the sediment, indicating the importance of sediment as source of gut bacteria in gibel carp. However, 37.95 % of the OTUs detected in feed were retrieved in the intestine, suggesting that food may influence markedly the microbiota of gibel carp, and therefore may be exploited for oral administration of probiotics.
关键词[WOS]: BACTERIAL-POPULATIONS ; GUT MICROBIOTA ; DIVERSITY ; IDENTIFICATION ; FISH ; AMPLIFICATION ; MICROFLORA ; LARVAE ; MUCUS ; DNA
语种: 英语
WOS记录号: WOS:000323223300005
ISSN号: 0959-3993
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19579
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
2.Natl Oceanog Ctr, Qingdao 266071, Shandong, Peoples R China
3.IFREMER, Ctr Brest, INRA, UR Nutr Metab & Aquaculture 1067, F-29280 Plouzane, France

Recommended Citation:
Wu, Shan-Gong; Tian, Jing-Yun; Gatesoupe, Francois-Joel; Li, Wen-Xiang; Zou, Hong; Yang, Bao-Juan; Wang, Gui-Tang.Intestinal microbiota of gibel carp (Carassius auratus gibelio) and its origin as revealed by 454 pyrosequencing,WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2013,29(9):1585-1595
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