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题名: Development of 201 tri- and tetra-nucleotide repeat microsatellites for bighead carp (Aristichthys nobilis)
作者: Zhu, Chuankun1, 2; Yu, Xiaomu1; Fu, B.1; Tong, Jingou1
通讯作者: Tong, JG (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.
关键词: Bighead carp (Aristichthys nobilis) ; Microsatellite ; Polymorphism ; Silver carp (Hypophthalmichthys molitrix) ; Cross-species amplification
刊名: CONSERVATION GENETICS RESOURCES
发表日期: 2013-09-01
DOI: 10.1007/s12686-013-9898-4
卷: 5, 期:3, 页:755-758
收录类别: SCI
文章类型: Article
部门归属: [Zhu, Chuankun ; Yu, Xiaomu ; Fu, B. ; Tong, Jingou] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China ; [Zhu, Chuankun] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: MOA [200903045]; NSFC [31272647]; MOST 973 [2010CB126305]; TGC [CT-12-08-01]; FEBL of China [2011FBZ20]
类目[WOS]: Biodiversity Conservation ; Genetics & Heredity
研究领域[WOS]: Biodiversity & Conservation ; Genetics & Heredity
摘要: In this study we developed 201 tri- and tetra-nucleotide repeat microsatellites for bighead carp (Aristichthys nobilis), and 135 of them showed polymorphism. Thirty-seven of polymorphic loci were randomly selected to characterize genetic diversity in a test population with 36 individuals. The number of alleles per locus ranged from 2 to 11 (mean 5.6), and the average observed and expected heterozygosity were 0.53 and 0.61, respectively. Twenty-four of the 37 loci were highly informative (PIC > 0.5) in the test population. Six loci deviated from the Hardy-Weinberg Equilibrium, and no pairs of loci were in linkage disequilibrium after Bonferroni correction. All 201 loci could be successfully amplified in silver carp (Hypophthalmichthys molitrix) with 64 loci being polymorphic, indicating a high universality of these microsatellites across species. These novel polynucleotide microsatellites would be useful tools for further population and conservation genetics studies in bighead carp and its closely-related silver carp.
英文摘要: In this study we developed 201 tri- and tetra-nucleotide repeat microsatellites for bighead carp (Aristichthys nobilis), and 135 of them showed polymorphism. Thirty-seven of polymorphic loci were randomly selected to characterize genetic diversity in a test population with 36 individuals. The number of alleles per locus ranged from 2 to 11 (mean 5.6), and the average observed and expected heterozygosity were 0.53 and 0.61, respectively. Twenty-four of the 37 loci were highly informative (PIC > 0.5) in the test population. Six loci deviated from the Hardy-Weinberg Equilibrium, and no pairs of loci were in linkage disequilibrium after Bonferroni correction. All 201 loci could be successfully amplified in silver carp (Hypophthalmichthys molitrix) with 64 loci being polymorphic, indicating a high universality of these microsatellites across species. These novel polynucleotide microsatellites would be useful tools for further population and conservation genetics studies in bighead carp and its closely-related silver carp.
语种: 英语
WOS记录号: WOS:000322619900037
ISSN号: 1877-7252
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19578
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Zhu, Chuankun; Yu, Xiaomu; Fu, B.; Tong, Jingou.Development of 201 tri- and tetra-nucleotide repeat microsatellites for bighead carp (Aristichthys nobilis),CONSERVATION GENETICS RESOURCES,2013,5(3):755-758
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