IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Development of 201 tri- and tetra-nucleotide repeat microsatellites for bighead carp (Aristichthys nobilis)
Zhu, Chuankun1,2; Yu, Xiaomu1; Fu, B.1; Tong, Jingou1; Tong, JG (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.
2013-09-01
Source PublicationCONSERVATION GENETICS RESOURCES
ISSN1877-7252
Volume5Issue:3Pages:755-758
AbstractIn this study we developed 201 tri- and tetra-nucleotide repeat microsatellites for bighead carp (Aristichthys nobilis), and 135 of them showed polymorphism. Thirty-seven of polymorphic loci were randomly selected to characterize genetic diversity in a test population with 36 individuals. The number of alleles per locus ranged from 2 to 11 (mean 5.6), and the average observed and expected heterozygosity were 0.53 and 0.61, respectively. Twenty-four of the 37 loci were highly informative (PIC > 0.5) in the test population. Six loci deviated from the Hardy-Weinberg Equilibrium, and no pairs of loci were in linkage disequilibrium after Bonferroni correction. All 201 loci could be successfully amplified in silver carp (Hypophthalmichthys molitrix) with 64 loci being polymorphic, indicating a high universality of these microsatellites across species. These novel polynucleotide microsatellites would be useful tools for further population and conservation genetics studies in bighead carp and its closely-related silver carp.; In this study we developed 201 tri- and tetra-nucleotide repeat microsatellites for bighead carp (Aristichthys nobilis), and 135 of them showed polymorphism. Thirty-seven of polymorphic loci were randomly selected to characterize genetic diversity in a test population with 36 individuals. The number of alleles per locus ranged from 2 to 11 (mean 5.6), and the average observed and expected heterozygosity were 0.53 and 0.61, respectively. Twenty-four of the 37 loci were highly informative (PIC > 0.5) in the test population. Six loci deviated from the Hardy-Weinberg Equilibrium, and no pairs of loci were in linkage disequilibrium after Bonferroni correction. All 201 loci could be successfully amplified in silver carp (Hypophthalmichthys molitrix) with 64 loci being polymorphic, indicating a high universality of these microsatellites across species. These novel polynucleotide microsatellites would be useful tools for further population and conservation genetics studies in bighead carp and its closely-related silver carp.
SubtypeArticle
KeywordBighead Carp (Aristichthys Nobilis) Microsatellite Polymorphism Silver Carp (Hypophthalmichthys Molitrix) Cross-species Amplification
Department[Zhu, Chuankun ; Yu, Xiaomu ; Fu, B. ; Tong, Jingou] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China ; [Zhu, Chuankun] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
DOI10.1007/s12686-013-9898-4
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationMOA [200903045]; NSFC [31272647]; MOST 973 [2010CB126305]; TGC [CT-12-08-01]; FEBL of China [2011FBZ20] ; MOA [200903045]; NSFC [31272647]; MOST 973 [2010CB126305]; TGC [CT-12-08-01]; FEBL of China [2011FBZ20] ; MOA [200903045]; NSFC [31272647]; MOST 973 [2010CB126305]; TGC [CT-12-08-01]; FEBL of China [2011FBZ20] ; MOA [200903045]; NSFC [31272647]; MOST 973 [2010CB126305]; TGC [CT-12-08-01]; FEBL of China [2011FBZ20]
Indexed BySCI
Language英语
WOS Research AreaBiodiversity & Conservation ; Genetics & Heredity
WOS SubjectBiodiversity Conservation ; Genetics & Heredity
WOS IDWOS:000322619900037
Funding OrganizationMOA [200903045]; NSFC [31272647]; MOST 973 [2010CB126305]; TGC [CT-12-08-01]; FEBL of China [2011FBZ20] ; MOA [200903045]; NSFC [31272647]; MOST 973 [2010CB126305]; TGC [CT-12-08-01]; FEBL of China [2011FBZ20] ; MOA [200903045]; NSFC [31272647]; MOST 973 [2010CB126305]; TGC [CT-12-08-01]; FEBL of China [2011FBZ20] ; MOA [200903045]; NSFC [31272647]; MOST 973 [2010CB126305]; TGC [CT-12-08-01]; FEBL of China [2011FBZ20]
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Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19578
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorTong, JG (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Chuankun,Yu, Xiaomu,Fu, B.,et al. Development of 201 tri- and tetra-nucleotide repeat microsatellites for bighead carp (Aristichthys nobilis)[J]. CONSERVATION GENETICS RESOURCES,2013,5(3):755-758.
APA Zhu, Chuankun,Yu, Xiaomu,Fu, B.,Tong, Jingou,&Tong, JG .(2013).Development of 201 tri- and tetra-nucleotide repeat microsatellites for bighead carp (Aristichthys nobilis).CONSERVATION GENETICS RESOURCES,5(3),755-758.
MLA Zhu, Chuankun,et al."Development of 201 tri- and tetra-nucleotide repeat microsatellites for bighead carp (Aristichthys nobilis)".CONSERVATION GENETICS RESOURCES 5.3(2013):755-758.
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