中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
题名: Evidence for Paralichthys olivaceus IFITM1 antiviral effect by impeding viral entry into target cells
作者: Zhu, Rong1; Wang, Jun1; Lei, Xiao-Ying1; Gui, Jian-Fang1; Zhang, Qi-Ya1
通讯作者: Zhang, QY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Donghu South Rd 7, Wuhan 430072, Peoples R China.
关键词: Paralichthys olivaceus interferon-inducible transmembrane 1 (PoIFITM1) ; Antiviral effect ; Rana grylio virus (RGV) ; Scophthalmus maximus rhabdovirus (SMRV) ; Golgi localization
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2013-09-01
DOI: 10.1016/j.fsi.2013.07.002
卷: 35, 期:3, 页:918-926
收录类别: SCI
文章类型: Article
部门归属: [Zhu, Rong ; Wang, Jun ; Lei, Xiao-Ying ; Gui, Jian-Fang ; Zhang, Qi-Ya] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Basic Research Program of China [2009CB118704, 2010CB126303]; National Natural Science Foundation of China [30800854, 31072231]
类目[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
摘要: Interferon-inducible transmembrane (IFITM) protein family is novel viral restriction factors with representative transmembrane structure. These proteins also exist in fish, however, their roles in the innate immune response remain unknown. Here, we report a characterization of teleost IFITM1 from flounder Paralichthys olivaceus (PoIFITM1), which exhibits conserved structure characteristic of the IFITM family but comprises a relatively longer N-terminal region. The expression and promoter activity of PoIFITM1 are markedly induced by aquatic animal viruses: Rana grylio virus (RGV) and Scophthalmus maximus rhabdovirus (SMRV). Overexpression and siRNA-mediated knockdown demonstrate that PoIFITM1 exhibits strong antiviral effects against both DNA virus (RGV) and RNA virus (SMRV), expanding the spectrum of viruses inhibited by IFITM proteins. Further analysis shows that PoIFITM1 suppresses viral entry into host cells, confirming that the IFITM-mediated restriction is conserved from lower vertebrates to mammals. Deletion mutagenesis reveals that PoIFITM1 exerts antiviral activity by targeting to Golgi complex and the N-terminal region is required for its subcellular localization, which is not observed in other known IFITM family members. Our current data provide the first evidence that IFITM1 functions as a key effector of the innate immune to restrict virus replication in lower vertebrates, through the action of impeding viral entry. (C) 2013 Elsevier Ltd. All rights reserved.
英文摘要: Interferon-inducible transmembrane (IFITM) protein family is novel viral restriction factors with representative transmembrane structure. These proteins also exist in fish, however, their roles in the innate immune response remain unknown. Here, we report a characterization of teleost IFITM1 from flounder Paralichthys olivaceus (PoIFITM1), which exhibits conserved structure characteristic of the IFITM family but comprises a relatively longer N-terminal region. The expression and promoter activity of PoIFITM1 are markedly induced by aquatic animal viruses: Rana grylio virus (RGV) and Scophthalmus maximus rhabdovirus (SMRV). Overexpression and siRNA-mediated knockdown demonstrate that PoIFITM1 exhibits strong antiviral effects against both DNA virus (RGV) and RNA virus (SMRV), expanding the spectrum of viruses inhibited by IFITM proteins. Further analysis shows that PoIFITM1 suppresses viral entry into host cells, confirming that the IFITM-mediated restriction is conserved from lower vertebrates to mammals. Deletion mutagenesis reveals that PoIFITM1 exerts antiviral activity by targeting to Golgi complex and the N-terminal region is required for its subcellular localization, which is not observed in other known IFITM family members. Our current data provide the first evidence that IFITM1 functions as a key effector of the innate immune to restrict virus replication in lower vertebrates, through the action of impeding viral entry. (C) 2013 Elsevier Ltd. All rights reserved.
关键词[WOS]: VIRUS-REPLICATION ; S-PALMITOYLATION ; INTERFERON ; INFLUENZA ; PROTEINS ; EXPRESSION ; IRIDOVIRUS ; RESISTANCE ; CLONING ; GENES
语种: 英语
WOS记录号: WOS:000324511700035
ISSN号: 1050-4648
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19576
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Evidence for Paralichthys olivaceus IFITM1 antiviral effect by impeding viral entry into target cells.pdf(2949KB)----开放获取View Download

作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

Recommended Citation:
Zhu, Rong; Wang, Jun; Lei, Xiao-Ying; Gui, Jian-Fang; Zhang, Qi-Ya.Evidence for Paralichthys olivaceus IFITM1 antiviral effect by impeding viral entry into target cells,FISH & SHELLFISH IMMUNOLOGY,2013,35(3):918-926
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhu, Rong]'s Articles
[Wang, Jun]'s Articles
[Lei, Xiao-Ying]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhu, Rong]‘s Articles
[Wang, Jun]‘s Articles
[Lei, Xiao-Ying]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Evidence for Paralichthys olivaceus IFITM1 antiviral effect by impeding viral entry into target cells.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院水生生物研究所 - Feedback
Powered by CSpace