IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Evidence for Paralichthys olivaceus IFITM1 antiviral effect by impeding viral entry into target cells
Zhu, Rong; Wang, Jun; Lei, Xiao-Ying; Gui, Jian-Fang; Zhang, Qi-Ya; Zhang, QY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Donghu South Rd 7, Wuhan 430072, Peoples R China.
2013-09-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume35Issue:3Pages:918-926
AbstractInterferon-inducible transmembrane (IFITM) protein family is novel viral restriction factors with representative transmembrane structure. These proteins also exist in fish, however, their roles in the innate immune response remain unknown. Here, we report a characterization of teleost IFITM1 from flounder Paralichthys olivaceus (PoIFITM1), which exhibits conserved structure characteristic of the IFITM family but comprises a relatively longer N-terminal region. The expression and promoter activity of PoIFITM1 are markedly induced by aquatic animal viruses: Rana grylio virus (RGV) and Scophthalmus maximus rhabdovirus (SMRV). Overexpression and siRNA-mediated knockdown demonstrate that PoIFITM1 exhibits strong antiviral effects against both DNA virus (RGV) and RNA virus (SMRV), expanding the spectrum of viruses inhibited by IFITM proteins. Further analysis shows that PoIFITM1 suppresses viral entry into host cells, confirming that the IFITM-mediated restriction is conserved from lower vertebrates to mammals. Deletion mutagenesis reveals that PoIFITM1 exerts antiviral activity by targeting to Golgi complex and the N-terminal region is required for its subcellular localization, which is not observed in other known IFITM family members. Our current data provide the first evidence that IFITM1 functions as a key effector of the innate immune to restrict virus replication in lower vertebrates, through the action of impeding viral entry. (C) 2013 Elsevier Ltd. All rights reserved.; Interferon-inducible transmembrane (IFITM) protein family is novel viral restriction factors with representative transmembrane structure. These proteins also exist in fish, however, their roles in the innate immune response remain unknown. Here, we report a characterization of teleost IFITM1 from flounder Paralichthys olivaceus (PoIFITM1), which exhibits conserved structure characteristic of the IFITM family but comprises a relatively longer N-terminal region. The expression and promoter activity of PoIFITM1 are markedly induced by aquatic animal viruses: Rana grylio virus (RGV) and Scophthalmus maximus rhabdovirus (SMRV). Overexpression and siRNA-mediated knockdown demonstrate that PoIFITM1 exhibits strong antiviral effects against both DNA virus (RGV) and RNA virus (SMRV), expanding the spectrum of viruses inhibited by IFITM proteins. Further analysis shows that PoIFITM1 suppresses viral entry into host cells, confirming that the IFITM-mediated restriction is conserved from lower vertebrates to mammals. Deletion mutagenesis reveals that PoIFITM1 exerts antiviral activity by targeting to Golgi complex and the N-terminal region is required for its subcellular localization, which is not observed in other known IFITM family members. Our current data provide the first evidence that IFITM1 functions as a key effector of the innate immune to restrict virus replication in lower vertebrates, through the action of impeding viral entry. (C) 2013 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordParalichthys Olivaceus Interferon-inducible Transmembrane 1 (Poifitm1) Antiviral Effect Rana Grylio Virus (Rgv) Scophthalmus Maximus Rhabdovirus (Smrv) Golgi Localization
Department[Zhu, Rong ; Wang, Jun ; Lei, Xiao-Ying ; Gui, Jian-Fang ; Zhang, Qi-Ya] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
DOI10.1016/j.fsi.2013.07.002
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Basic Research Program of China [2009CB118704, 2010CB126303]; National Natural Science Foundation of China [30800854, 31072231] ; National Basic Research Program of China [2009CB118704, 2010CB126303]; National Natural Science Foundation of China [30800854, 31072231] ; National Basic Research Program of China [2009CB118704, 2010CB126303]; National Natural Science Foundation of China [30800854, 31072231] ; National Basic Research Program of China [2009CB118704, 2010CB126303]; National Natural Science Foundation of China [30800854, 31072231]
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000324511700035
WOS KeywordVIRUS-REPLICATION ; S-PALMITOYLATION ; INTERFERON ; INFLUENZA ; PROTEINS ; EXPRESSION ; IRIDOVIRUS ; RESISTANCE ; CLONING ; GENES
Funding OrganizationNational Basic Research Program of China [2009CB118704, 2010CB126303]; National Natural Science Foundation of China [30800854, 31072231] ; National Basic Research Program of China [2009CB118704, 2010CB126303]; National Natural Science Foundation of China [30800854, 31072231] ; National Basic Research Program of China [2009CB118704, 2010CB126303]; National Natural Science Foundation of China [30800854, 31072231] ; National Basic Research Program of China [2009CB118704, 2010CB126303]; National Natural Science Foundation of China [30800854, 31072231]
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19576
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorZhang, QY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Donghu South Rd 7, Wuhan 430072, Peoples R China.
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhu, Rong,Wang, Jun,Lei, Xiao-Ying,et al. Evidence for Paralichthys olivaceus IFITM1 antiviral effect by impeding viral entry into target cells[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(3):918-926.
APA Zhu, Rong,Wang, Jun,Lei, Xiao-Ying,Gui, Jian-Fang,Zhang, Qi-Ya,&Zhang, QY .(2013).Evidence for Paralichthys olivaceus IFITM1 antiviral effect by impeding viral entry into target cells.FISH & SHELLFISH IMMUNOLOGY,35(3),918-926.
MLA Zhu, Rong,et al."Evidence for Paralichthys olivaceus IFITM1 antiviral effect by impeding viral entry into target cells".FISH & SHELLFISH IMMUNOLOGY 35.3(2013):918-926.
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