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Isolation and characterization of a novel antibacterial peptide derived from hemoglobin alpha in the liver of Japanese eel, Anguilla japonica
Zhang, Dong Ling1,2,3; Guan, Rui Zhang1,2; Huang, Wen Shu2; Xiong, Jing2; Guan, RZ (reprint author), Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China.
2013-09-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume35Issue:3Pages:625-631
AbstractWe isolated and characterized a novel antibacterial peptide, AJHb alpha, derived from hemoglobin alpha in the liver of Japanese eel, Anguilla japonica. It with concentration of 11.30 mu M exhibited stronger antibacterial activity against pathogenic bacterium 1 x 10(6) cell ml(-1) Edwardsiella tarda than other two bacteria. The extraction procedure for AJHb alpha included extraction with acetate acid, ultrafiltration, cation-exchange chromatography on HiTrap (TM) CM FF, reverse-phase liquid chromatography on Source 5R RPC and 08 RP-HPLC. MALDI-TOF MS suggested that the peptide had an observed molecular weight of 2388.05 Da. Its amino acid sequence determined by Edman degradation was similar to those of hemoglobin alpha chain in other fish by BLAST analysis. A complete N-terminal amino acid sequence of the AJHba was FAHWPDLGPGSPSVKKHGKVIM corresponding to the cDNA sequence by RACE amplification. Its synthetic peptide had strong antibacterial activities against ten Gram-positive or negative bacteria. To our knowledge, AJHba was the first identified fragment of hemoglobin alpha chain with strong antibacterial activity in fish. (C) 2012 Elsevier Ltd. All rights reserved.; We isolated and characterized a novel antibacterial peptide, AJHb alpha, derived from hemoglobin alpha in the liver of Japanese eel, Anguilla japonica. It with concentration of 11.30 mu M exhibited stronger antibacterial activity against pathogenic bacterium 1 x 10(6) cell ml(-1) Edwardsiella tarda than other two bacteria. The extraction procedure for AJHb alpha included extraction with acetate acid, ultrafiltration, cation-exchange chromatography on HiTrap (TM) CM FF, reverse-phase liquid chromatography on Source 5R RPC and 08 RP-HPLC. MALDI-TOF MS suggested that the peptide had an observed molecular weight of 2388.05 Da. Its amino acid sequence determined by Edman degradation was similar to those of hemoglobin alpha chain in other fish by BLAST analysis. A complete N-terminal amino acid sequence of the AJHba was FAHWPDLGPGSPSVKKHGKVIM corresponding to the cDNA sequence by RACE amplification. Its synthetic peptide had strong antibacterial activities against ten Gram-positive or negative bacteria. To our knowledge, AJHba was the first identified fragment of hemoglobin alpha chain with strong antibacterial activity in fish. (C) 2012 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordAntibacterial Peptide Hemoglobin Alpha Chain Japanese Eel Anguilla Japonica Liver
Department[Zhang, Dong Ling ; Guan, Rui Zhang] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China ; [Zhang, Dong Ling ; Guan, Rui Zhang ; Huang, Wen Shu ; Xiong, Jing] Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China ; [Zhang, Dong Ling] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
DOI10.1016/j.fsi.2012.08.022
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationAgricultural eel Public Welfare research project [nyhyzx07-043]; Department of Education of Fujian Province [JA09157]; Hall of Fujian Province Science and Technology platform construction project [2009N2003-1]; Fujian Province Science and Technology project [2010N0022] ; Agricultural eel Public Welfare research project [nyhyzx07-043]; Department of Education of Fujian Province [JA09157]; Hall of Fujian Province Science and Technology platform construction project [2009N2003-1]; Fujian Province Science and Technology project [2010N0022] ; Agricultural eel Public Welfare research project [nyhyzx07-043]; Department of Education of Fujian Province [JA09157]; Hall of Fujian Province Science and Technology platform construction project [2009N2003-1]; Fujian Province Science and Technology project [2010N0022] ; Agricultural eel Public Welfare research project [nyhyzx07-043]; Department of Education of Fujian Province [JA09157]; Hall of Fujian Province Science and Technology platform construction project [2009N2003-1]; Fujian Province Science and Technology project [2010N0022]
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000324511700001
WOS KeywordRED-SEA BREAM ; ANTIMICROBIAL PEPTIDES ; ONCORHYNCHUS-MYKISS ; RAINBOW-TROUT ; DEFENSE PEPTIDES ; EPIDERMAL MUCUS ; HISTONE H2A ; SKIN MUCUS ; CELLS ; FISH
Funding OrganizationAgricultural eel Public Welfare research project [nyhyzx07-043]; Department of Education of Fujian Province [JA09157]; Hall of Fujian Province Science and Technology platform construction project [2009N2003-1]; Fujian Province Science and Technology project [2010N0022] ; Agricultural eel Public Welfare research project [nyhyzx07-043]; Department of Education of Fujian Province [JA09157]; Hall of Fujian Province Science and Technology platform construction project [2009N2003-1]; Fujian Province Science and Technology project [2010N0022] ; Agricultural eel Public Welfare research project [nyhyzx07-043]; Department of Education of Fujian Province [JA09157]; Hall of Fujian Province Science and Technology platform construction project [2009N2003-1]; Fujian Province Science and Technology project [2010N0022] ; Agricultural eel Public Welfare research project [nyhyzx07-043]; Department of Education of Fujian Province [JA09157]; Hall of Fujian Province Science and Technology platform construction project [2009N2003-1]; Fujian Province Science and Technology project [2010N0022]
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19575
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorGuan, RZ (reprint author), Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
2.Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Dong Ling,Guan, Rui Zhang,Huang, Wen Shu,et al. Isolation and characterization of a novel antibacterial peptide derived from hemoglobin alpha in the liver of Japanese eel, Anguilla japonica[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(3):625-631.
APA Zhang, Dong Ling,Guan, Rui Zhang,Huang, Wen Shu,Xiong, Jing,&Guan, RZ .(2013).Isolation and characterization of a novel antibacterial peptide derived from hemoglobin alpha in the liver of Japanese eel, Anguilla japonica.FISH & SHELLFISH IMMUNOLOGY,35(3),625-631.
MLA Zhang, Dong Ling,et al."Isolation and characterization of a novel antibacterial peptide derived from hemoglobin alpha in the liver of Japanese eel, Anguilla japonica".FISH & SHELLFISH IMMUNOLOGY 35.3(2013):625-631.
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