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Roles of plasmid-encoded proteins, EseH, EseI and EscD in invasion, replication and virulence of Edwardsiella ictaluri
Zhao, Li Juan1,2; Lu, Jin Fang2; Nie, P.2,3; Li, Ai Hua2; Xiong, Bang Xi1; Xie, Hai Xia2; Nie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China.
2013-09-27
Source PublicationVETERINARY MICROBIOLOGY
ISSN0378-1135
Volume166Issue:1-2Pages:233-241
AbstractNative plasmids pEI1 and pEI2 were detected in Edwardsiella ictaluri HSN-1 isolated from diseased yellow catfish (Pelteobagrus fulvidraco). EseH encoded by pEI1 and other two proteins, EseI and EscD, encoded by pEI2, were found with homology to type III secretion system (T3SS) proteins. To investigate their roles in pathogenesis, the native plasmids were cured based on plasmid incompatibility by introducing a Kan positive and SacB negative selection marker into gene spacer of the native plasmids. Mutants with the deletion of the target genes were obtained by reverse PCR and self-ligation, and all mutants were examined for their virulence effect in yellow catfish. Compared with the HSN-1 strain, the two mutants Delta eseH and Delta eseI were attenuated, while mutant Delta escD had increased virulence with higher Competitive Index (CI) value. The adherence and invasion assays on fish EPC cells indicated that Delta eseH and Delta eseI had decreased ability in adherence. Using E. tarda as surrogate, EseH and EseI were detected in culture supernatants, but EscD was not, with the secretion of EseH depending on T3SS. In addition, EseH and EseI were found translocated into host cells, and by means of subcellular fractionation, EseH was localized in membrane fraction of ZF4 cells, and EseI in the cytosol fraction. Hence, the role of these three genes in adherence, invasion and cellular replication was revealed from the pathogenic bacterium E. ictaluri. (C) 2013 Elsevier B.V. All rights reserved.; Native plasmids pEI1 and pEI2 were detected in Edwardsiella ictaluri HSN-1 isolated from diseased yellow catfish (Pelteobagrus fulvidraco). EseH encoded by pEI1 and other two proteins, EseI and EscD, encoded by pEI2, were found with homology to type III secretion system (T3SS) proteins. To investigate their roles in pathogenesis, the native plasmids were cured based on plasmid incompatibility by introducing a Kan positive and SacB negative selection marker into gene spacer of the native plasmids. Mutants with the deletion of the target genes were obtained by reverse PCR and self-ligation, and all mutants were examined for their virulence effect in yellow catfish. Compared with the HSN-1 strain, the two mutants Delta eseH and Delta eseI were attenuated, while mutant Delta escD had increased virulence with higher Competitive Index (CI) value. The adherence and invasion assays on fish EPC cells indicated that Delta eseH and Delta eseI had decreased ability in adherence. Using E. tarda as surrogate, EseH and EseI were detected in culture supernatants, but EscD was not, with the secretion of EseH depending on T3SS. In addition, EseH and EseI were found translocated into host cells, and by means of subcellular fractionation, EseH was localized in membrane fraction of ZF4 cells, and EseI in the cytosol fraction. Hence, the role of these three genes in adherence, invasion and cellular replication was revealed from the pathogenic bacterium E. ictaluri. (C) 2013 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordPlasmid Eseh Esei Escd Virulence Edwardsiella Ictaluri
Department[Zhao, Li Juan ; Xiong, Bang Xi] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei Province, Peoples R China ; [Zhao, Li Juan ; Lu, Jin Fang ; Nie, P. ; Li, Ai Hua ; Xie, Hai Xia] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China ; [Nie, P.] Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China
DOI10.1016/j.vetmic.2013.05.023
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China (NSFC) [30972278, 31172442]; National Basic Research Program of China (973program) [2009CB118703]; Jimei University of Fujian Province ; National Natural Science Foundation of China (NSFC) [30972278, 31172442]; National Basic Research Program of China (973program) [2009CB118703]; Jimei University of Fujian Province ; National Natural Science Foundation of China (NSFC) [30972278, 31172442]; National Basic Research Program of China (973program) [2009CB118703]; Jimei University of Fujian Province ; National Natural Science Foundation of China (NSFC) [30972278, 31172442]; National Basic Research Program of China (973program) [2009CB118703]; Jimei University of Fujian Province
Indexed BySCI
Language英语
WOS Research AreaMicrobiology ; Veterinary Sciences
WOS SubjectMicrobiology ; Veterinary Sciences
WOS IDWOS:000322848100026
WOS KeywordIII SECRETION SYSTEM ; CATFISH PELTEOBAGRUS-FULVIDRACO ; COMPLETE DNA-SEQUENCE ; EPC CELL-LINE ; ENTERIC SEPTICEMIA ; CHANNEL CATFISH ; BETA-LACTAMASE ; EFFECTOR ; TARDA ; TRANSLOCATION
Funding OrganizationNational Natural Science Foundation of China (NSFC) [30972278, 31172442]; National Basic Research Program of China (973program) [2009CB118703]; Jimei University of Fujian Province ; National Natural Science Foundation of China (NSFC) [30972278, 31172442]; National Basic Research Program of China (973program) [2009CB118703]; Jimei University of Fujian Province ; National Natural Science Foundation of China (NSFC) [30972278, 31172442]; National Basic Research Program of China (973program) [2009CB118703]; Jimei University of Fujian Province ; National Natural Science Foundation of China (NSFC) [30972278, 31172442]; National Basic Research Program of China (973program) [2009CB118703]; Jimei University of Fujian Province
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Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19574
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorNie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China.
Affiliation1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei Province, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
3.Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Li Juan,Lu, Jin Fang,Nie, P.,et al. Roles of plasmid-encoded proteins, EseH, EseI and EscD in invasion, replication and virulence of Edwardsiella ictaluri[J]. VETERINARY MICROBIOLOGY,2013,166(1-2):233-241.
APA Zhao, Li Juan.,Lu, Jin Fang.,Nie, P..,Li, Ai Hua.,Xiong, Bang Xi.,...&Nie, P .(2013).Roles of plasmid-encoded proteins, EseH, EseI and EscD in invasion, replication and virulence of Edwardsiella ictaluri.VETERINARY MICROBIOLOGY,166(1-2),233-241.
MLA Zhao, Li Juan,et al."Roles of plasmid-encoded proteins, EseH, EseI and EscD in invasion, replication and virulence of Edwardsiella ictaluri".VETERINARY MICROBIOLOGY 166.1-2(2013):233-241.
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