IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Genome architecture changes and major gene variations of Andrias davidianus ranavirus (ADRV)
Chen, Zhongyuan1; Gui, Jianfang1; Gao, Xiaochan1; Pei, Chao1; Hong, Yijiang2; Zhang, Qiya1; Zhang, QY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
2013-10-21
Source PublicationVETERINARY RESEARCH
ISSN0928-4249
Volume44Pages:101
AbstractRanaviruses are emerging pathogens that have led to global impact and public concern. As a rarely endangered species and the largest amphibian in the world, the Chinese giant salamander, Andrias davidianus, has recently undergone outbreaks of epidemic diseases with high mortality. In this study, we isolated and identified a novel ranavirus from the Chinese giant salamanders that exhibited systemic hemorrhage and swelling syndrome with high death rate in China during May 2011 to August 2012. The isolate, designated Andrias davidianus ranavirus (ADRV), not only could induce cytopathic effects in different fish cell lines and yield high viral titers, but also caused severely hemorrhagic lesions and resulted in 100% mortality in experimental infections of salamanders. The complete genome of ADRV was sequenced and compared with other sequenced amphibian ranaviruses. Gene content and phylogenetic analyses revealed that ADRV should belong to an amphibian subgroup in genus Ranavirus, and is more closely related to frog ranaviruses than to other salamander ranaviruses. Homologous gene comparisons show that ADRV contains 99%, 97%, 94%, 93% and 85% homologues in RGV, FV3, CMTV, TFV and ATV genomes respectively. In addition, several variable major genes, such as duplicate US22 family-like genes, viral eukaryotic translation initiation factor 2 alpha gene and novel 75L gene with both motifs of nuclear localization signal (NLS) and nuclear export signal (NES), were predicted to contribute to pathogen virulence and host susceptibility. These findings confirm the etiologic role of ADRV in epidemic diseases of Chinese giant salamanders, and broaden our understanding of evolutionary emergence of ranaviruses.; Ranaviruses are emerging pathogens that have led to global impact and public concern. As a rarely endangered species and the largest amphibian in the world, the Chinese giant salamander, Andrias davidianus, has recently undergone outbreaks of epidemic diseases with high mortality. In this study, we isolated and identified a novel ranavirus from the Chinese giant salamanders that exhibited systemic hemorrhage and swelling syndrome with high death rate in China during May 2011 to August 2012. The isolate, designated Andrias davidianus ranavirus (ADRV), not only could induce cytopathic effects in different fish cell lines and yield high viral titers, but also caused severely hemorrhagic lesions and resulted in 100% mortality in experimental infections of salamanders. The complete genome of ADRV was sequenced and compared with other sequenced amphibian ranaviruses. Gene content and phylogenetic analyses revealed that ADRV should belong to an amphibian subgroup in genus Ranavirus, and is more closely related to frog ranaviruses than to other salamander ranaviruses. Homologous gene comparisons show that ADRV contains 99%, 97%, 94%, 93% and 85% homologues in RGV, FV3, CMTV, TFV and ATV genomes respectively. In addition, several variable major genes, such as duplicate US22 family-like genes, viral eukaryotic translation initiation factor 2 alpha gene and novel 75L gene with both motifs of nuclear localization signal (NLS) and nuclear export signal (NES), were predicted to contribute to pathogen virulence and host susceptibility. These findings confirm the etiologic role of ADRV in epidemic diseases of Chinese giant salamanders, and broaden our understanding of evolutionary emergence of ranaviruses.
SubtypeArticle
KeywordRana-grylio Virus Fish Cell-line Family-iridoviridae Viral Replication Nuclear Import Frog Sequence Protein Export Nucleoprotein
Department[Chen, Zhongyuan ; Gui, Jianfang ; Gao, Xiaochan ; Pei, Chao ; Zhang, Qiya] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Hong, Yijiang] Nanchang Univ, Coll Life Sci & Food Engn, Nanchang 330031, Peoples R China
DOI10.1186/1297-9716-44-101
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Major Basic Research Program [2010CB126303]; National Natural Science Foundation of China [31072239, 31270213, 31202028]; Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; Key Projects in the National Science and Technology Pillar Program during the Twelve Five-Year Plan Period [2012BAD25B0202] ; National Major Basic Research Program(2010CB126303) ; National Major Basic Research Program [2010CB126303]; National Natural Science Foundation of China [31072239, 31270213, 31202028]; Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; Key Projects in the National Science and Technology Pillar Program during the Twelve Five-Year Plan Period [2012BAD25B0202] ; National Major Basic Research Program(2010CB126303) ; National Natural Science Foundation of China(31072239 ; National Natural Science Foundation of China(31072239 ; Program of the Chinese Academy of Sciences(KSCX2-EW-Z-3) ; Program of the Chinese Academy of Sciences(KSCX2-EW-Z-3) ; Key Projects in the National Science and Technology Pillar Program during the Twelve Five-Year Plan Period(2012BAD25B0202) ; Key Projects in the National Science and Technology Pillar Program during the Twelve Five-Year Plan Period(2012BAD25B0202) ; 31270213 ; 31270213 ; 31202028) ; 31202028) ; National Major Basic Research Program [2010CB126303]; National Natural Science Foundation of China [31072239, 31270213, 31202028]; Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; Key Projects in the National Science and Technology Pillar Program during the Twelve Five-Year Plan Period [2012BAD25B0202] ; National Major Basic Research Program(2010CB126303) ; National Major Basic Research Program [2010CB126303]; National Natural Science Foundation of China [31072239, 31270213, 31202028]; Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; Key Projects in the National Science and Technology Pillar Program during the Twelve Five-Year Plan Period [2012BAD25B0202] ; National Major Basic Research Program(2010CB126303) ; National Natural Science Foundation of China(31072239 ; National Natural Science Foundation of China(31072239 ; Program of the Chinese Academy of Sciences(KSCX2-EW-Z-3) ; Program of the Chinese Academy of Sciences(KSCX2-EW-Z-3) ; Key Projects in the National Science and Technology Pillar Program during the Twelve Five-Year Plan Period(2012BAD25B0202) ; Key Projects in the National Science and Technology Pillar Program during the Twelve Five-Year Plan Period(2012BAD25B0202) ; 31270213 ; 31270213 ; 31202028) ; 31202028)
Indexed BySCI
Language英语
WOS Research AreaVeterinary Sciences
WOS SubjectVeterinary Sciences
WOS IDWOS:000326186900001
WOS KeywordRANA-GRYLIO VIRUS ; FISH CELL-LINE ; FAMILY-IRIDOVIRIDAE ; VIRAL REPLICATION ; NUCLEAR IMPORT ; FROG ; SEQUENCE ; PROTEIN ; EXPORT ; NUCLEOPROTEIN
Funding OrganizationNational Major Basic Research Program [2010CB126303]; National Natural Science Foundation of China [31072239, 31270213, 31202028]; Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; Key Projects in the National Science and Technology Pillar Program during the Twelve Five-Year Plan Period [2012BAD25B0202] ; National Major Basic Research Program(2010CB126303) ; National Major Basic Research Program [2010CB126303]; National Natural Science Foundation of China [31072239, 31270213, 31202028]; Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; Key Projects in the National Science and Technology Pillar Program during the Twelve Five-Year Plan Period [2012BAD25B0202] ; National Major Basic Research Program(2010CB126303) ; National Natural Science Foundation of China(31072239 ; National Natural Science Foundation of China(31072239 ; Program of the Chinese Academy of Sciences(KSCX2-EW-Z-3) ; Program of the Chinese Academy of Sciences(KSCX2-EW-Z-3) ; Key Projects in the National Science and Technology Pillar Program during the Twelve Five-Year Plan Period(2012BAD25B0202) ; Key Projects in the National Science and Technology Pillar Program during the Twelve Five-Year Plan Period(2012BAD25B0202) ; 31270213 ; 31270213 ; 31202028) ; 31202028) ; National Major Basic Research Program [2010CB126303]; National Natural Science Foundation of China [31072239, 31270213, 31202028]; Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; Key Projects in the National Science and Technology Pillar Program during the Twelve Five-Year Plan Period [2012BAD25B0202] ; National Major Basic Research Program(2010CB126303) ; National Major Basic Research Program [2010CB126303]; National Natural Science Foundation of China [31072239, 31270213, 31202028]; Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; Key Projects in the National Science and Technology Pillar Program during the Twelve Five-Year Plan Period [2012BAD25B0202] ; National Major Basic Research Program(2010CB126303) ; National Natural Science Foundation of China(31072239 ; National Natural Science Foundation of China(31072239 ; Program of the Chinese Academy of Sciences(KSCX2-EW-Z-3) ; Program of the Chinese Academy of Sciences(KSCX2-EW-Z-3) ; Key Projects in the National Science and Technology Pillar Program during the Twelve Five-Year Plan Period(2012BAD25B0202) ; Key Projects in the National Science and Technology Pillar Program during the Twelve Five-Year Plan Period(2012BAD25B0202) ; 31270213 ; 31270213 ; 31202028) ; 31202028)
Citation statistics
Cited Times:39[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19570
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorZhang, QY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Nanchang Univ, Coll Life Sci & Food Engn, Nanchang 330031, Peoples R China
Recommended Citation
GB/T 7714
Chen, Zhongyuan,Gui, Jianfang,Gao, Xiaochan,et al. Genome architecture changes and major gene variations of Andrias davidianus ranavirus (ADRV)[J]. VETERINARY RESEARCH,2013,44:101.
APA Chen, Zhongyuan.,Gui, Jianfang.,Gao, Xiaochan.,Pei, Chao.,Hong, Yijiang.,...&Zhang, QY .(2013).Genome architecture changes and major gene variations of Andrias davidianus ranavirus (ADRV).VETERINARY RESEARCH,44,101.
MLA Chen, Zhongyuan,et al."Genome architecture changes and major gene variations of Andrias davidianus ranavirus (ADRV)".VETERINARY RESEARCH 44(2013):101.
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