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题名: Genome architecture changes and major gene variations of Andrias davidianus ranavirus (ADRV)
作者: Chen, Zhongyuan1; Gui, Jianfang1; Gao, Xiaochan1; Pei, Chao1; Hong, Yijiang2; Zhang, Qiya1
通讯作者: Zhang, QY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
关键词: RANA-GRYLIO VIRUS ; FISH CELL-LINE ; FAMILY-IRIDOVIRIDAE ; VIRAL REPLICATION ; NUCLEAR IMPORT ; FROG ; SEQUENCE ; PROTEIN ; EXPORT ; NUCLEOPROTEIN
刊名: VETERINARY RESEARCH
发表日期: 2013-10-21
DOI: 10.1186/1297-9716-44-101
卷: 44, 页:101
收录类别: SCI
文章类型: Article
部门归属: [Chen, Zhongyuan ; Gui, Jianfang ; Gao, Xiaochan ; Pei, Chao ; Zhang, Qiya] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; [Hong, Yijiang] Nanchang Univ, Coll Life Sci & Food Engn, Nanchang 330031, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Major Basic Research Program [2010CB126303]; National Natural Science Foundation of China [31072239, 31270213, 31202028]; Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; Key Projects in the National Science and Technology Pillar Program during the Twelve Five-Year Plan Period [2012BAD25B0202]
类目[WOS]: Veterinary Sciences
研究领域[WOS]: Veterinary Sciences
摘要: Ranaviruses are emerging pathogens that have led to global impact and public concern. As a rarely endangered species and the largest amphibian in the world, the Chinese giant salamander, Andrias davidianus, has recently undergone outbreaks of epidemic diseases with high mortality. In this study, we isolated and identified a novel ranavirus from the Chinese giant salamanders that exhibited systemic hemorrhage and swelling syndrome with high death rate in China during May 2011 to August 2012. The isolate, designated Andrias davidianus ranavirus (ADRV), not only could induce cytopathic effects in different fish cell lines and yield high viral titers, but also caused severely hemorrhagic lesions and resulted in 100% mortality in experimental infections of salamanders. The complete genome of ADRV was sequenced and compared with other sequenced amphibian ranaviruses. Gene content and phylogenetic analyses revealed that ADRV should belong to an amphibian subgroup in genus Ranavirus, and is more closely related to frog ranaviruses than to other salamander ranaviruses. Homologous gene comparisons show that ADRV contains 99%, 97%, 94%, 93% and 85% homologues in RGV, FV3, CMTV, TFV and ATV genomes respectively. In addition, several variable major genes, such as duplicate US22 family-like genes, viral eukaryotic translation initiation factor 2 alpha gene and novel 75L gene with both motifs of nuclear localization signal (NLS) and nuclear export signal (NES), were predicted to contribute to pathogen virulence and host susceptibility. These findings confirm the etiologic role of ADRV in epidemic diseases of Chinese giant salamanders, and broaden our understanding of evolutionary emergence of ranaviruses.
英文摘要: Ranaviruses are emerging pathogens that have led to global impact and public concern. As a rarely endangered species and the largest amphibian in the world, the Chinese giant salamander, Andrias davidianus, has recently undergone outbreaks of epidemic diseases with high mortality. In this study, we isolated and identified a novel ranavirus from the Chinese giant salamanders that exhibited systemic hemorrhage and swelling syndrome with high death rate in China during May 2011 to August 2012. The isolate, designated Andrias davidianus ranavirus (ADRV), not only could induce cytopathic effects in different fish cell lines and yield high viral titers, but also caused severely hemorrhagic lesions and resulted in 100% mortality in experimental infections of salamanders. The complete genome of ADRV was sequenced and compared with other sequenced amphibian ranaviruses. Gene content and phylogenetic analyses revealed that ADRV should belong to an amphibian subgroup in genus Ranavirus, and is more closely related to frog ranaviruses than to other salamander ranaviruses. Homologous gene comparisons show that ADRV contains 99%, 97%, 94%, 93% and 85% homologues in RGV, FV3, CMTV, TFV and ATV genomes respectively. In addition, several variable major genes, such as duplicate US22 family-like genes, viral eukaryotic translation initiation factor 2 alpha gene and novel 75L gene with both motifs of nuclear localization signal (NLS) and nuclear export signal (NES), were predicted to contribute to pathogen virulence and host susceptibility. These findings confirm the etiologic role of ADRV in epidemic diseases of Chinese giant salamanders, and broaden our understanding of evolutionary emergence of ranaviruses.
关键词[WOS]: RANA-GRYLIO VIRUS ; FISH CELL-LINE ; FAMILY-IRIDOVIRIDAE ; VIRAL REPLICATION ; NUCLEAR IMPORT ; FROG ; SEQUENCE ; PROTEIN ; EXPORT ; NUCLEOPROTEIN
语种: 英语
项目资助者: National Major Basic Research Program(2010CB126303) ; National Natural Science Foundation of China(31072239 ; Program of the Chinese Academy of Sciences(KSCX2-EW-Z-3) ; Key Projects in the National Science and Technology Pillar Program during the Twelve Five-Year Plan Period(2012BAD25B0202) ; 31270213 ; 31202028)
WOS记录号: WOS:000326186900001
ISSN号: 0928-4249
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19570
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Nanchang Univ, Coll Life Sci & Food Engn, Nanchang 330031, Peoples R China

Recommended Citation:
Chen, Zhongyuan; Gui, Jianfang; Gao, Xiaochan; Pei, Chao; Hong, Yijiang; Zhang, Qiya.Genome architecture changes and major gene variations of Andrias davidianus ranavirus (ADRV),VETERINARY RESEARCH,2013,44():101
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