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Phylogenetic analysis of vertebrate CXC chemokines reveals novel lineage specific groups in teleost fish
Chen, Jun1,2; Xu, Qiaoqing1,3; Wang, Tiehui1; Collet, Bertrand4; Corripio-Miyar, Yolanda1; Bird, Steve1,5; Xie, Ping2; Nie, Pin2; Secombes, Christopher J.1; Zou, Jun1; Secombes, CJ (reprint author), Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2TZ, Scotland.
2013-10-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume41Issue:2Pages:137-152
AbstractIn this study, we have identified 421 molecules across the vertebrate spectrum and propose a unified nomenclature for CXC chemokines in fish, amphibians and reptiles based on phylogenetic analysis. Expanding on earlier studies in teleost fish, lineage specific CXC chemokines that have no apparent homologues in mammals were confirmed. Furthermore, in addition to the two subgroups of the CXCL8 homologues known in teleost fish, a third group was identified (termed CXCL8_L3), as was a further subgroup of the fish CXC genes related to CXCL11. Expression of the CXC chemokines found in rainbow trout, Oncorhynchus mykiss, was studied in response to stimulation with inflammatory and antiviral cytokines, and bacterial. Tissue distribution analysis revealed distinct expression profiles for these trout CXC chemokines. Lastly three of the trout chemokines, including two novel fish specific CXC chemokines containing three pairs of cysteines, were produced as recombinant proteins and their effect on trout leucocyte migration studied. These molecules increased the relative expression of CD4 and MCSFR in migrated cells in an in vitro chemotaxis assay. (C) 2013 Elsevier Ltd. All rights reserved.; In this study, we have identified 421 molecules across the vertebrate spectrum and propose a unified nomenclature for CXC chemokines in fish, amphibians and reptiles based on phylogenetic analysis. Expanding on earlier studies in teleost fish, lineage specific CXC chemokines that have no apparent homologues in mammals were confirmed. Furthermore, in addition to the two subgroups of the CXCL8 homologues known in teleost fish, a third group was identified (termed CXCL8_L3), as was a further subgroup of the fish CXC genes related to CXCL11. Expression of the CXC chemokines found in rainbow trout, Oncorhynchus mykiss, was studied in response to stimulation with inflammatory and antiviral cytokines, and bacterial. Tissue distribution analysis revealed distinct expression profiles for these trout CXC chemokines. Lastly three of the trout chemokines, including two novel fish specific CXC chemokines containing three pairs of cysteines, were produced as recombinant proteins and their effect on trout leucocyte migration studied. These molecules increased the relative expression of CD4 and MCSFR in migrated cells in an in vitro chemotaxis assay. (C) 2013 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordFish Teleosts Vertebrates Cxc Chemokines
Department[Chen, Jun ; Xu, Qiaoqing ; Wang, Tiehui ; Corripio-Miyar, Yolanda ; Bird, Steve ; Secombes, Christopher J. ; Zou, Jun] Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2TZ, Scotland ; [Chen, Jun ; Xie, Ping ; Nie, Pin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China ; [Xu, Qiaoqing] Yangtze Univ, Sch Anim Sci, Jinzhou 434020, Hubei Province, Peoples R China ; [Collet, Bertrand] Marine Scotland Sci, Aberdeen AB11 9DB, Scotland ; [Bird, Steve] Univ Waikato, Sch Biol Sci, Dept Sci & Engn, Hamilton 3240, New Zealand
DOI10.1016/j.dci.2013.05.006
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationRoyal Society of Edinburgh; National Natural Science Foundation of China; Leverhulme Trust; 973 National Basic Research Program of China [2009CB118703] ; Royal Society of Edinburgh; National Natural Science Foundation of China; Leverhulme Trust; 973 National Basic Research Program of China [2009CB118703] ; Royal Society of Edinburgh; National Natural Science Foundation of China; Leverhulme Trust; 973 National Basic Research Program of China [2009CB118703] ; Royal Society of Edinburgh; National Natural Science Foundation of China; Leverhulme Trust; 973 National Basic Research Program of China [2009CB118703]
Indexed BySCI
Language英语
WOS Research AreaImmunology ; Zoology
WOS SubjectImmunology ; Zoology
WOS IDWOS:000323870800005
WOS KeywordTROUT ONCORHYNCHUS-MYKISS ; PRO-INFLAMMATORY CYTOKINES ; RAINBOW-TROUT ; MOLECULAR CHARACTERIZATION ; EDWARDSIELLA-ICTALURI ; EXPRESSION ANALYSIS ; CC CHEMOKINES ; IFN-GAMMA ; IDENTIFICATION ; INTERLEUKIN-8
Funding OrganizationRoyal Society of Edinburgh; National Natural Science Foundation of China; Leverhulme Trust; 973 National Basic Research Program of China [2009CB118703] ; Royal Society of Edinburgh; National Natural Science Foundation of China; Leverhulme Trust; 973 National Basic Research Program of China [2009CB118703] ; Royal Society of Edinburgh; National Natural Science Foundation of China; Leverhulme Trust; 973 National Basic Research Program of China [2009CB118703] ; Royal Society of Edinburgh; National Natural Science Foundation of China; Leverhulme Trust; 973 National Basic Research Program of China [2009CB118703]
Citation statistics
Cited Times:31[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19569
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorSecombes, CJ (reprint author), Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2TZ, Scotland.
Affiliation1.Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2TZ, Scotland
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
3.Yangtze Univ, Sch Anim Sci, Jinzhou 434020, Hubei Province, Peoples R China
4.Marine Scotland Sci, Aberdeen AB11 9DB, Scotland
5.Univ Waikato, Sch Biol Sci, Dept Sci & Engn, Hamilton 3240, New Zealand
Recommended Citation
GB/T 7714
Chen, Jun,Xu, Qiaoqing,Wang, Tiehui,et al. Phylogenetic analysis of vertebrate CXC chemokines reveals novel lineage specific groups in teleost fish[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2013,41(2):137-152.
APA Chen, Jun.,Xu, Qiaoqing.,Wang, Tiehui.,Collet, Bertrand.,Corripio-Miyar, Yolanda.,...&Secombes, CJ .(2013).Phylogenetic analysis of vertebrate CXC chemokines reveals novel lineage specific groups in teleost fish.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,41(2),137-152.
MLA Chen, Jun,et al."Phylogenetic analysis of vertebrate CXC chemokines reveals novel lineage specific groups in teleost fish".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 41.2(2013):137-152.
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