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题名: Tetrahymena-specific biomass evaluation with a DNA-based method
作者: Yan, Qingyun1; Yu, Yuhe1; Dai, Lili1, 2
通讯作者: Yu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China.
关键词: genomic DNA ; real-time quantitative PCR ; molecular methods
刊名: SCIENCEASIA
发表日期: 2013-02-01
DOI: 10.2306/scienceasia1513-1874.2013.39.036
卷: 39, 期:1, 页:36-41
收录类别: SCI
文章类型: Article
部门归属: [Yan, Qingyun ; Yu, Yuhe ; Dai, Lili] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China ; [Dai, Lili] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
WOS标题词: Science & Technology
资助者: National Natural Science Foundation of China [31071896]; Knowledge Innovation Program of the Chinese Academy of Sciences [Y15E04]; Key Laboratory of Marine and Estuarine Fisheries Resources and Ecology, Ministry of Agriculture [201007]; Youth Innovation Promotion Association, CAS [Y22Z07]
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
摘要: Evaluating the biomass of a specific taxon or that of a certain nutritional level is especially important for understanding an ecosystem. Although various methods are available, there is still a lack of a universally accepted approach for taxa-specific biomass evaluation. Taking cultured unicellular Tetrahymena as an example, the present study is aimed at exploring and highlighting a DNA-based method for taxa-specific biomass estimation. Results indicated that the Tetrahymena DNA yield increased linearly with increasing number of Tetrahymena cells (p<0.001). The intra and inter real-time quantitative PCR (Q-PCR) assays were highly reproducible, and Q-PCR quantifying 18S rDNA could detect an equivalent quantity of a single cell or less. Tetrahymena-specific biomass then can be determined according to the rDNA copies quantified by Q-PCR (p < 0.002), considering both of species richness and evenness (indicated by the pooled cells of Tetrahymena species with different ratios). Observations of the present study together with some others suggest that it is possible to quantify a target micro-biomass in natural ambience via specifically-amplified genes.
英文摘要: Evaluating the biomass of a specific taxon or that of a certain nutritional level is especially important for understanding an ecosystem. Although various methods are available, there is still a lack of a universally accepted approach for taxa-specific biomass evaluation. Taking cultured unicellular Tetrahymena as an example, the present study is aimed at exploring and highlighting a DNA-based method for taxa-specific biomass estimation. Results indicated that the Tetrahymena DNA yield increased linearly with increasing number of Tetrahymena cells (p<0.001). The intra and inter real-time quantitative PCR (Q-PCR) assays were highly reproducible, and Q-PCR quantifying 18S rDNA could detect an equivalent quantity of a single cell or less. Tetrahymena-specific biomass then can be determined according to the rDNA copies quantified by Q-PCR (p < 0.002), considering both of species richness and evenness (indicated by the pooled cells of Tetrahymena species with different ratios). Observations of the present study together with some others suggest that it is possible to quantify a target micro-biomass in natural ambience via specifically-amplified genes.
关键词[WOS]: REAL-TIME PCR ; QUANTITATIVE PCR ; ENVIRONMENTAL-SAMPLES ; MOLECULAR ECOLOGY ; MICROBIAL BIOMASS ; BACTERIAL-DNA ; QUANTIFICATION ; GENE ; SOIL ; SEA
语种: 英语
WOS记录号: WOS:000324222400005
ISSN号: 1513-1874
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19562
Appears in Collections:淡水生态学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

Recommended Citation:
Yan, Qingyun; Yu, Yuhe; Dai, Lili.Tetrahymena-specific biomass evaluation with a DNA-based method,SCIENCEASIA,2013,39(1):36-41
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