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Tetrahymena-specific biomass evaluation with a DNA-based method
Yan, Qingyun1; Yu, Yuhe1; Dai, Lili1,2; Yu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China.
2013-02-01
Source PublicationSCIENCEASIA
ISSN1513-1874
Volume39Issue:1Pages:36-41
AbstractEvaluating the biomass of a specific taxon or that of a certain nutritional level is especially important for understanding an ecosystem. Although various methods are available, there is still a lack of a universally accepted approach for taxa-specific biomass evaluation. Taking cultured unicellular Tetrahymena as an example, the present study is aimed at exploring and highlighting a DNA-based method for taxa-specific biomass estimation. Results indicated that the Tetrahymena DNA yield increased linearly with increasing number of Tetrahymena cells (p<0.001). The intra and inter real-time quantitative PCR (Q-PCR) assays were highly reproducible, and Q-PCR quantifying 18S rDNA could detect an equivalent quantity of a single cell or less. Tetrahymena-specific biomass then can be determined according to the rDNA copies quantified by Q-PCR (p < 0.002), considering both of species richness and evenness (indicated by the pooled cells of Tetrahymena species with different ratios). Observations of the present study together with some others suggest that it is possible to quantify a target micro-biomass in natural ambience via specifically-amplified genes.; Evaluating the biomass of a specific taxon or that of a certain nutritional level is especially important for understanding an ecosystem. Although various methods are available, there is still a lack of a universally accepted approach for taxa-specific biomass evaluation. Taking cultured unicellular Tetrahymena as an example, the present study is aimed at exploring and highlighting a DNA-based method for taxa-specific biomass estimation. Results indicated that the Tetrahymena DNA yield increased linearly with increasing number of Tetrahymena cells (p<0.001). The intra and inter real-time quantitative PCR (Q-PCR) assays were highly reproducible, and Q-PCR quantifying 18S rDNA could detect an equivalent quantity of a single cell or less. Tetrahymena-specific biomass then can be determined according to the rDNA copies quantified by Q-PCR (p < 0.002), considering both of species richness and evenness (indicated by the pooled cells of Tetrahymena species with different ratios). Observations of the present study together with some others suggest that it is possible to quantify a target micro-biomass in natural ambience via specifically-amplified genes.
SubtypeArticle
KeywordGenomic Dna Real-time Quantitative Pcr Molecular Methods
Department[Yan, Qingyun ; Yu, Yuhe ; Dai, Lili] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China ; [Dai, Lili] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
DOI10.2306/scienceasia1513-1874.2013.39.036
WOS HeadingsScience & Technology
Funding OrganizationNational Natural Science Foundation of China [31071896]; Knowledge Innovation Program of the Chinese Academy of Sciences [Y15E04]; Key Laboratory of Marine and Estuarine Fisheries Resources and Ecology, Ministry of Agriculture [201007]; Youth Innovation Promotion Association, CAS [Y22Z07] ; National Natural Science Foundation of China [31071896]; Knowledge Innovation Program of the Chinese Academy of Sciences [Y15E04]; Key Laboratory of Marine and Estuarine Fisheries Resources and Ecology, Ministry of Agriculture [201007]; Youth Innovation Promotion Association, CAS [Y22Z07] ; National Natural Science Foundation of China [31071896]; Knowledge Innovation Program of the Chinese Academy of Sciences [Y15E04]; Key Laboratory of Marine and Estuarine Fisheries Resources and Ecology, Ministry of Agriculture [201007]; Youth Innovation Promotion Association, CAS [Y22Z07] ; National Natural Science Foundation of China [31071896]; Knowledge Innovation Program of the Chinese Academy of Sciences [Y15E04]; Key Laboratory of Marine and Estuarine Fisheries Resources and Ecology, Ministry of Agriculture [201007]; Youth Innovation Promotion Association, CAS [Y22Z07]
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000324222400005
WOS KeywordREAL-TIME PCR ; QUANTITATIVE PCR ; ENVIRONMENTAL-SAMPLES ; MOLECULAR ECOLOGY ; MICROBIAL BIOMASS ; BACTERIAL-DNA ; QUANTIFICATION ; GENE ; SOIL ; SEA
Funding OrganizationNational Natural Science Foundation of China [31071896]; Knowledge Innovation Program of the Chinese Academy of Sciences [Y15E04]; Key Laboratory of Marine and Estuarine Fisheries Resources and Ecology, Ministry of Agriculture [201007]; Youth Innovation Promotion Association, CAS [Y22Z07] ; National Natural Science Foundation of China [31071896]; Knowledge Innovation Program of the Chinese Academy of Sciences [Y15E04]; Key Laboratory of Marine and Estuarine Fisheries Resources and Ecology, Ministry of Agriculture [201007]; Youth Innovation Promotion Association, CAS [Y22Z07] ; National Natural Science Foundation of China [31071896]; Knowledge Innovation Program of the Chinese Academy of Sciences [Y15E04]; Key Laboratory of Marine and Estuarine Fisheries Resources and Ecology, Ministry of Agriculture [201007]; Youth Innovation Promotion Association, CAS [Y22Z07] ; National Natural Science Foundation of China [31071896]; Knowledge Innovation Program of the Chinese Academy of Sciences [Y15E04]; Key Laboratory of Marine and Estuarine Fisheries Resources and Ecology, Ministry of Agriculture [201007]; Youth Innovation Promotion Association, CAS [Y22Z07]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19562
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorYu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Yan, Qingyun,Yu, Yuhe,Dai, Lili,et al. Tetrahymena-specific biomass evaluation with a DNA-based method[J]. SCIENCEASIA,2013,39(1):36-41.
APA Yan, Qingyun,Yu, Yuhe,Dai, Lili,&Yu, YH .(2013).Tetrahymena-specific biomass evaluation with a DNA-based method.SCIENCEASIA,39(1),36-41.
MLA Yan, Qingyun,et al."Tetrahymena-specific biomass evaluation with a DNA-based method".SCIENCEASIA 39.1(2013):36-41.
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