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Gut Microbiota Contributes to the Growth of Fast-Growing Transgenic Common Carp (Cyprinus carpio L.)
Li, Xuemei1,4; Yan, Qingyun1,2; Xie, Shouqi2; Hu, Wei2; Yu, Yuhe1; Hu, Zihua3; Yu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China.
2013-05-31
Source PublicationPLOS ONE
ISSN1932-6203
Volume8Issue:5Pages:e64577
AbstractGut microbiota has shown tight and coordinated connection with various functions of its host such as metabolism, immunity, energy utilization, and health maintenance. To gain insight into whether gut microbes affect the metabolism of fish, we employed fast-growing transgenic common carp (Cyprinus carpio L.) to study the connections between its large body feature and gut microbes. Metagenome-based fingerprinting and high-throughput sequencing on bacterial 16S rRNA genes indicated that fish gut was dominated by Proteobacteria, Fusobacteria, Bacteroidetes and Firmicutes, which displayed significant differences between transgenic fish and wild-type controls. Analyses to study the association of gut microbes with the fish metabolism discovered three major phyla having significant relationships with the host metabolic factors. Biochemical and histological analyses indicated transgenic fish had increased carbohydrate but decreased lipid metabolisms. Additionally, transgenic fish has a significantly lower Bacteroidetes: Firmicutes ratio than that of wild-type controls, which is similar to mammals between obese and lean individuals. These findings suggest that gut microbiotas are associated with the growth of fast growing transgenic fish, and the relative abundance of Firmicutes over Bacteroidetes could be one of the factors contributing to its fast growth. Since the large body size of transgenic fish displays a proportional body growth, which is unlike obesity inhuman, the results together with the findings from others also suggest that the link between obesity and gut microbiota is likely more complex than a simple Bacteroidetes: Firmicutes ratio change.; Gut microbiota has shown tight and coordinated connection with various functions of its host such as metabolism, immunity, energy utilization, and health maintenance. To gain insight into whether gut microbes affect the metabolism of fish, we employed fast-growing transgenic common carp (Cyprinus carpio L.) to study the connections between its large body feature and gut microbes. Metagenome-based fingerprinting and high-throughput sequencing on bacterial 16S rRNA genes indicated that fish gut was dominated by Proteobacteria, Fusobacteria, Bacteroidetes and Firmicutes, which displayed significant differences between transgenic fish and wild-type controls. Analyses to study the association of gut microbes with the fish metabolism discovered three major phyla having significant relationships with the host metabolic factors. Biochemical and histological analyses indicated transgenic fish had increased carbohydrate but decreased lipid metabolisms. Additionally, transgenic fish has a significantly lower Bacteroidetes: Firmicutes ratio than that of wild-type controls, which is similar to mammals between obese and lean individuals. These findings suggest that gut microbiotas are associated with the growth of fast growing transgenic fish, and the relative abundance of Firmicutes over Bacteroidetes could be one of the factors contributing to its fast growth. Since the large body size of transgenic fish displays a proportional body growth, which is unlike obesity inhuman, the results together with the findings from others also suggest that the link between obesity and gut microbiota is likely more complex than a simple Bacteroidetes: Firmicutes ratio change.
SubtypeArticle
KeywordGradient Gel-electrophoresis Diet-induced Obesity 16s Ribosomal-rna Host Metabolism Weight-loss Zebrafish Energy Mice Communities Absorption
Department[Li, Xuemei ; Yan, Qingyun ; Yu, Yuhe] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China ; [Yan, Qingyun ; Xie, Shouqi ; Hu, Wei] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China ; [Hu, Zihua] SUNY Buffalo, New York State Ctr Excellence Bioinformat & Life, Ctr Computat Res, Dept Ophthalmol,Dept Biostat,Dept Med, Buffalo, NY 14260 USA ; [Li, Xuemei] Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Minist Agr China, Key Lab Freshwater Biodivers Conservat, Wuhan, Peoples R China
DOI10.1371/journal.pone.0064577
WOS HeadingsScience & Technology
Funding OrganizationMajor State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [31172084, 31071896]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB03]; Knowledge Innovation Program of the Chinese Academy of Sciences [Y15E04]; Youth Innovation Promotion Association, CAS [Y22Z07] ; Major State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [31172084, 31071896]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB03]; Knowledge Innovation Program of the Chinese Academy of Sciences [Y15E04]; Youth Innovation Promotion Association, CAS [Y22Z07] ; Major State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [31172084, 31071896]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB03]; Knowledge Innovation Program of the Chinese Academy of Sciences [Y15E04]; Youth Innovation Promotion Association, CAS [Y22Z07] ; Major State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [31172084, 31071896]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB03]; Knowledge Innovation Program of the Chinese Academy of Sciences [Y15E04]; Youth Innovation Promotion Association, CAS [Y22Z07]
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000319799900057
WOS KeywordGRADIENT GEL-ELECTROPHORESIS ; DIET-INDUCED OBESITY ; 16S RIBOSOMAL-RNA ; HOST METABOLISM ; WEIGHT-LOSS ; ZEBRAFISH ; ENERGY ; MICE ; COMMUNITIES ; ABSORPTION
Funding OrganizationMajor State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [31172084, 31071896]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB03]; Knowledge Innovation Program of the Chinese Academy of Sciences [Y15E04]; Youth Innovation Promotion Association, CAS [Y22Z07] ; Major State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [31172084, 31071896]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB03]; Knowledge Innovation Program of the Chinese Academy of Sciences [Y15E04]; Youth Innovation Promotion Association, CAS [Y22Z07] ; Major State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [31172084, 31071896]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB03]; Knowledge Innovation Program of the Chinese Academy of Sciences [Y15E04]; Youth Innovation Promotion Association, CAS [Y22Z07] ; Major State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [31172084, 31071896]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB03]; Knowledge Innovation Program of the Chinese Academy of Sciences [Y15E04]; Youth Innovation Promotion Association, CAS [Y22Z07]
Citation statistics
Cited Times:39[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19558
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorYu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
3.SUNY Buffalo, New York State Ctr Excellence Bioinformat & Life, Ctr Computat Res, Dept Ophthalmol,Dept Biostat,Dept Med, Buffalo, NY 14260 USA
4.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Minist Agr China, Key Lab Freshwater Biodivers Conservat, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Li, Xuemei,Yan, Qingyun,Xie, Shouqi,et al. Gut Microbiota Contributes to the Growth of Fast-Growing Transgenic Common Carp (Cyprinus carpio L.)[J]. PLOS ONE,2013,8(5):e64577.
APA Li, Xuemei.,Yan, Qingyun.,Xie, Shouqi.,Hu, Wei.,Yu, Yuhe.,...&Yu, YH .(2013).Gut Microbiota Contributes to the Growth of Fast-Growing Transgenic Common Carp (Cyprinus carpio L.).PLOS ONE,8(5),e64577.
MLA Li, Xuemei,et al."Gut Microbiota Contributes to the Growth of Fast-Growing Transgenic Common Carp (Cyprinus carpio L.)".PLOS ONE 8.5(2013):e64577.
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