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题名: Gut Microbiota Contributes to the Growth of Fast-Growing Transgenic Common Carp (Cyprinus carpio L.)
作者: Li, Xuemei1, 4; Yan, Qingyun1, 2; Xie, Shouqi2; Hu, Wei2; Yu, Yuhe1; Hu, Zihua3
通讯作者: Yu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China.
关键词: GRADIENT GEL-ELECTROPHORESIS ; DIET-INDUCED OBESITY ; 16S RIBOSOMAL-RNA ; HOST METABOLISM ; WEIGHT-LOSS ; ZEBRAFISH ; ENERGY ; MICE ; COMMUNITIES ; ABSORPTION
刊名: PLOS ONE
发表日期: 2013-05-31
DOI: 10.1371/journal.pone.0064577
卷: 8, 期:5, 页:e64577
收录类别: SCI
文章类型: Article
部门归属: [Li, Xuemei ; Yan, Qingyun ; Yu, Yuhe] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China ; [Yan, Qingyun ; Xie, Shouqi ; Hu, Wei] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China ; [Hu, Zihua] SUNY Buffalo, New York State Ctr Excellence Bioinformat & Life, Ctr Computat Res, Dept Ophthalmol,Dept Biostat,Dept Med, Buffalo, NY 14260 USA ; [Li, Xuemei] Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Minist Agr China, Key Lab Freshwater Biodivers Conservat, Wuhan, Peoples R China
WOS标题词: Science & Technology
资助者: Major State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [31172084, 31071896]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB03]; Knowledge Innovation Program of the Chinese Academy of Sciences [Y15E04]; Youth Innovation Promotion Association, CAS [Y22Z07]
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
摘要: Gut microbiota has shown tight and coordinated connection with various functions of its host such as metabolism, immunity, energy utilization, and health maintenance. To gain insight into whether gut microbes affect the metabolism of fish, we employed fast-growing transgenic common carp (Cyprinus carpio L.) to study the connections between its large body feature and gut microbes. Metagenome-based fingerprinting and high-throughput sequencing on bacterial 16S rRNA genes indicated that fish gut was dominated by Proteobacteria, Fusobacteria, Bacteroidetes and Firmicutes, which displayed significant differences between transgenic fish and wild-type controls. Analyses to study the association of gut microbes with the fish metabolism discovered three major phyla having significant relationships with the host metabolic factors. Biochemical and histological analyses indicated transgenic fish had increased carbohydrate but decreased lipid metabolisms. Additionally, transgenic fish has a significantly lower Bacteroidetes: Firmicutes ratio than that of wild-type controls, which is similar to mammals between obese and lean individuals. These findings suggest that gut microbiotas are associated with the growth of fast growing transgenic fish, and the relative abundance of Firmicutes over Bacteroidetes could be one of the factors contributing to its fast growth. Since the large body size of transgenic fish displays a proportional body growth, which is unlike obesity inhuman, the results together with the findings from others also suggest that the link between obesity and gut microbiota is likely more complex than a simple Bacteroidetes: Firmicutes ratio change.
英文摘要: Gut microbiota has shown tight and coordinated connection with various functions of its host such as metabolism, immunity, energy utilization, and health maintenance. To gain insight into whether gut microbes affect the metabolism of fish, we employed fast-growing transgenic common carp (Cyprinus carpio L.) to study the connections between its large body feature and gut microbes. Metagenome-based fingerprinting and high-throughput sequencing on bacterial 16S rRNA genes indicated that fish gut was dominated by Proteobacteria, Fusobacteria, Bacteroidetes and Firmicutes, which displayed significant differences between transgenic fish and wild-type controls. Analyses to study the association of gut microbes with the fish metabolism discovered three major phyla having significant relationships with the host metabolic factors. Biochemical and histological analyses indicated transgenic fish had increased carbohydrate but decreased lipid metabolisms. Additionally, transgenic fish has a significantly lower Bacteroidetes: Firmicutes ratio than that of wild-type controls, which is similar to mammals between obese and lean individuals. These findings suggest that gut microbiotas are associated with the growth of fast growing transgenic fish, and the relative abundance of Firmicutes over Bacteroidetes could be one of the factors contributing to its fast growth. Since the large body size of transgenic fish displays a proportional body growth, which is unlike obesity inhuman, the results together with the findings from others also suggest that the link between obesity and gut microbiota is likely more complex than a simple Bacteroidetes: Firmicutes ratio change.
关键词[WOS]: GRADIENT GEL-ELECTROPHORESIS ; DIET-INDUCED OBESITY ; 16S RIBOSOMAL-RNA ; HOST METABOLISM ; WEIGHT-LOSS ; ZEBRAFISH ; ENERGY ; MICE ; COMMUNITIES ; ABSORPTION
语种: 英语
WOS记录号: WOS:000319799900057
ISSN号: 1932-6203
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19558
Appears in Collections:淡水生态学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
3.SUNY Buffalo, New York State Ctr Excellence Bioinformat & Life, Ctr Computat Res, Dept Ophthalmol,Dept Biostat,Dept Med, Buffalo, NY 14260 USA
4.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Minist Agr China, Key Lab Freshwater Biodivers Conservat, Wuhan, Peoples R China

Recommended Citation:
Li, Xuemei; Yan, Qingyun; Xie, Shouqi; Hu, Wei; Yu, Yuhe; Hu, Zihua.Gut Microbiota Contributes to the Growth of Fast-Growing Transgenic Common Carp (Cyprinus carpio L.),PLOS ONE,2013,8(5):e64577
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