| How much metagenomic sequencing is enough to achieve a given goal? | |
| Ni, Jiajia1,2; Yan, Qingyun1; Yu, Yuhe1; Yu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China. | |
| 2013-06-11 | |
| Source Publication | SCIENTIFIC REPORTS
 |
| ISSN | 2045-2322 |
| Volume | 3Issue:- |
| Abstract | Metagenomic studies have dramatically expanded our knowledge of the microbial world. Furthermore, the amount of sample for sequencing has significantly increased with the development of high-throughput sequencing technologies. However, fully capturing all DNA sequences carried by every microorganism in the environment is still impossible. Therefore, estimating a reasonable and practical amount for sequencing to achieve the objectives is particularly necessary. In the present study, we introduce a novel method for estimating the required minimum amount for metagenomic sequencing for a given goal. We also calculated the genomic proportion of each operational taxonomic unit and the detection efficiency of a specific gene (we have used SSU rRNA gene as an example) based on a given amount for random metagenomic sequencing. The reasonable and practical estimated amount for sequencing in metagenomic studies will provide good reference information when applying high-throughput sequencing for a given goal.; Metagenomic studies have dramatically expanded our knowledge of the microbial world. Furthermore, the amount of sample for sequencing has significantly increased with the development of high-throughput sequencing technologies. However, fully capturing all DNA sequences carried by every microorganism in the environment is still impossible. Therefore, estimating a reasonable and practical amount for sequencing to achieve the objectives is particularly necessary. In the present study, we introduce a novel method for estimating the required minimum amount for metagenomic sequencing for a given goal. We also calculated the genomic proportion of each operational taxonomic unit and the detection efficiency of a specific gene (we have used SSU rRNA gene as an example) based on a given amount for random metagenomic sequencing. The reasonable and practical estimated amount for sequencing in metagenomic studies will provide good reference information when applying high-throughput sequencing for a given goal. |
| Subtype | Article |
| Keyword | Microbial Diversity Gut Microbiome Deep-sea Biosphere Communities |
| Department | [Ni, Jiajia; Yan, Qingyun; Yu, Yuhe] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China; [Ni, Jiajia] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| DOI | 10.1038/srep01968 |
| WOS Headings | Science & Technology |
| Funding Organization | Major State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [30970358, 31172084] ; Major State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [30970358, 31172084] ; Major State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [30970358, 31172084] ; Major State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [30970358, 31172084] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Science & Technology - Other Topics |
| WOS Subject | Multidisciplinary Sciences |
| WOS ID | WOS:000320130300007 |
| WOS Keyword | MICROBIAL DIVERSITY ; GUT MICROBIOME ; DEEP-SEA ; BIOSPHERE ; COMMUNITIES |
| Funding Organization | Major State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [30970358, 31172084] ; Major State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [30970358, 31172084] ; Major State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [30970358, 31172084] ; Major State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [30970358, 31172084] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/19556 |
| Collection | 淡水生态学研究中心_期刊论文 |
| Corresponding Author | Yu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China. |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Ni, Jiajia,Yan, Qingyun,Yu, Yuhe,et al. How much metagenomic sequencing is enough to achieve a given goal?[J]. SCIENTIFIC REPORTS,2013,3(-). |
| APA | Ni, Jiajia,Yan, Qingyun,Yu, Yuhe,&Yu, YH .(2013).How much metagenomic sequencing is enough to achieve a given goal?.SCIENTIFIC REPORTS,3(-). |
| MLA | Ni, Jiajia,et al."How much metagenomic sequencing is enough to achieve a given goal?".SCIENTIFIC REPORTS 3.-(2013). |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| How much metagenomic（995KB） | 开放获取 | CC BY-NC-SA | View Application Full Text | |||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment