中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 淡水生态学研究中心  > 期刊论文
题名: How much metagenomic sequencing is enough to achieve a given goal?
作者: Ni, Jiajia1, 2; Yan, Qingyun1; Yu, Yuhe1
通讯作者: Yu, YH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China.
关键词: MICROBIAL DIVERSITY ; GUT MICROBIOME ; DEEP-SEA ; BIOSPHERE ; COMMUNITIES
刊名: SCIENTIFIC REPORTS
发表日期: 2013-06-11
DOI: 10.1038/srep01968
卷: 3, 期:-
收录类别: SCI
文章类型: Article
部门归属: [Ni, Jiajia; Yan, Qingyun; Yu, Yuhe] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China; [Ni, Jiajia] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
WOS标题词: Science & Technology
资助者: Major State Basic Research Development Program of China [2009CB118705]; National Natural Science Foundation of China [30970358, 31172084]
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
摘要: Metagenomic studies have dramatically expanded our knowledge of the microbial world. Furthermore, the amount of sample for sequencing has significantly increased with the development of high-throughput sequencing technologies. However, fully capturing all DNA sequences carried by every microorganism in the environment is still impossible. Therefore, estimating a reasonable and practical amount for sequencing to achieve the objectives is particularly necessary. In the present study, we introduce a novel method for estimating the required minimum amount for metagenomic sequencing for a given goal. We also calculated the genomic proportion of each operational taxonomic unit and the detection efficiency of a specific gene (we have used SSU rRNA gene as an example) based on a given amount for random metagenomic sequencing. The reasonable and practical estimated amount for sequencing in metagenomic studies will provide good reference information when applying high-throughput sequencing for a given goal.
英文摘要: Metagenomic studies have dramatically expanded our knowledge of the microbial world. Furthermore, the amount of sample for sequencing has significantly increased with the development of high-throughput sequencing technologies. However, fully capturing all DNA sequences carried by every microorganism in the environment is still impossible. Therefore, estimating a reasonable and practical amount for sequencing to achieve the objectives is particularly necessary. In the present study, we introduce a novel method for estimating the required minimum amount for metagenomic sequencing for a given goal. We also calculated the genomic proportion of each operational taxonomic unit and the detection efficiency of a specific gene (we have used SSU rRNA gene as an example) based on a given amount for random metagenomic sequencing. The reasonable and practical estimated amount for sequencing in metagenomic studies will provide good reference information when applying high-throughput sequencing for a given goal.
关键词[WOS]: MICROBIAL DIVERSITY ; GUT MICROBIOME ; DEEP-SEA ; BIOSPHERE ; COMMUNITIES
语种: 英语
项目资助者: Major State Basic Research Development Program of China(2009CB118705) ; National Natural Science Foundation of China(30970358 ; 31172084)
WOS记录号: WOS:000320130300007
ISSN号: 2045-2322
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19556
Appears in Collections:淡水生态学研究中心_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
How much metagenomic sequencing is enough to achieve a given goal_.pdf(995KB)----开放获取View 联系获取全文

作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Ni, Jiajia]'s Articles
[Yan, Qingyun]'s Articles
[Yu, Yuhe]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Ni, Jiajia]‘s Articles
[Yan, Qingyun]‘s Articles
[Yu, Yuhe]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: How much metagenomic sequencing is enough to achieve a given goal_.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院水生生物研究所 - Feedback
Powered by CSpace