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题名: Renal accumulation and effects of intraperitoneal injection of extracted microcystins in omnivorous crucian carp (Carassius auratus)
作者: Li, Li1; Xie, Ping2; Lei, Hehua3; Zhang, Xuezhen1
通讯作者: Li, L (reprint author), Huazhong Agr Univ, Coll Fisheries, Minist Agr, Key Lab Freshwater Anim Breeding, Wuhan 430070, Peoples R China.
关键词: Microcystins ; Nephrotoxic ; Intraperitoneal injection ; Crucian carp ; Ultrastructural changes
刊名: TOXICON
发表日期: 2013-08-01
DOI: 10.1016/j.toxicon.2013.03.022
卷: 70, 页:62-69
收录类别: SCI
文章类型: Article
部门归属: [Li, Li ; Zhang, Xuezhen] Huazhong Agr Univ, Coll Fisheries, Minist Agr, Key Lab Freshwater Anim Breeding, Wuhan 430070, Peoples R China ; [Xie, Ping] Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China ; [Lei, Hehua] Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [31100378]; Fundamental Research Funds for the Central Universities [2010QC022]
类目[WOS]: Pharmacology & Pharmacy ; Toxicology
研究领域[WOS]: Pharmacology & Pharmacy ; Toxicology
摘要: An acute toxicological experiment was designed to characterize the sequence of renal ultrastructural changes with accumulated MCs in crucian carp injected intraperitoneally (i.p.) with extracted microcystins (mainly MC-RR and -LR) at two doses, 50 and 200 mu g MC-LReq. kg(-1) body weight. Quantitative and qualitative determinations of MCs in the kidney were conducted by HPLC and LC-MS, respectively. MC-RR content in kidney of crucian carp showed a time dose-dependent increase within 48 h post-injection, followed by a sharp decline afterward, while no MC-LR in kidney was detectable throughout the experiment. Ultrastructural changes in the kidney of crucian carp progressed with increasing accumulated MCs and exposure times within 48 h post-injection, whereas renal ultrastructural recovery of crucian carp in the 50 mu g MC-LReq. kg(-1) dose group was evident at 168 h post-injection. Our ultrastructural observation suggests that the membranous structure is the main action site of MCs in the kidney, among which mitochondria damage in the tubules is clearly an early, and presumably a critically important effect of MCs. The increases in blood urea nitrogen (BUN) and creatinine (CR) in both dose groups further revealed severe impairment occurred in the kidney of crucian carp. (C) 2013 Elsevier Ltd. All rights reserved.
英文摘要: An acute toxicological experiment was designed to characterize the sequence of renal ultrastructural changes with accumulated MCs in crucian carp injected intraperitoneally (i.p.) with extracted microcystins (mainly MC-RR and -LR) at two doses, 50 and 200 mu g MC-LReq. kg(-1) body weight. Quantitative and qualitative determinations of MCs in the kidney were conducted by HPLC and LC-MS, respectively. MC-RR content in kidney of crucian carp showed a time dose-dependent increase within 48 h post-injection, followed by a sharp decline afterward, while no MC-LR in kidney was detectable throughout the experiment. Ultrastructural changes in the kidney of crucian carp progressed with increasing accumulated MCs and exposure times within 48 h post-injection, whereas renal ultrastructural recovery of crucian carp in the 50 mu g MC-LReq. kg(-1) dose group was evident at 168 h post-injection. Our ultrastructural observation suggests that the membranous structure is the main action site of MCs in the kidney, among which mitochondria damage in the tubules is clearly an early, and presumably a critically important effect of MCs. The increases in blood urea nitrogen (BUN) and creatinine (CR) in both dose groups further revealed severe impairment occurred in the kidney of crucian carp. (C) 2013 Elsevier Ltd. All rights reserved.
关键词[WOS]: PHYTOPLANKTIVOROUS SILVER CARP ; TOXIC CYANOBACTERIAL BLOOMS ; DIFFERENT TROPHIC LEVELS ; FISH OREOCHROMIS SP. ; LABORATORY CONDITIONS ; BIGHEAD CARP ; IN-SITU ; BIOCHEMICAL-CHARACTERIZATION ; TISSUE DISTRIBUTION ; OXIDATIVE STRESS
语种: 英语
WOS记录号: WOS:000321537200008
ISSN号: 0041-0101
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19551
Appears in Collections:淡水生态学研究中心_期刊论文

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作者单位: 1.Huazhong Agr Univ, Coll Fisheries, Minist Agr, Key Lab Freshwater Anim Breeding, Wuhan 430070, Peoples R China
2.Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China
3.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan 430072, Peoples R China

Recommended Citation:
Li, Li; Xie, Ping; Lei, Hehua; Zhang, Xuezhen.Renal accumulation and effects of intraperitoneal injection of extracted microcystins in omnivorous crucian carp (Carassius auratus),TOXICON,2013,70():62-69
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