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Renal accumulation and effects of intraperitoneal injection of extracted microcystins in omnivorous crucian carp (Carassius auratus)
Li, Li1; Xie, Ping2; Lei, Hehua3; Zhang, Xuezhen1; Li, L (reprint author), Huazhong Agr Univ, Coll Fisheries, Minist Agr, Key Lab Freshwater Anim Breeding, Wuhan 430070, Peoples R China.
2013-08-01
Source PublicationTOXICON
ISSN0041-0101
Volume70Pages:62-69
AbstractAn acute toxicological experiment was designed to characterize the sequence of renal ultrastructural changes with accumulated MCs in crucian carp injected intraperitoneally (i.p.) with extracted microcystins (mainly MC-RR and -LR) at two doses, 50 and 200 mu g MC-LReq. kg(-1) body weight. Quantitative and qualitative determinations of MCs in the kidney were conducted by HPLC and LC-MS, respectively. MC-RR content in kidney of crucian carp showed a time dose-dependent increase within 48 h post-injection, followed by a sharp decline afterward, while no MC-LR in kidney was detectable throughout the experiment. Ultrastructural changes in the kidney of crucian carp progressed with increasing accumulated MCs and exposure times within 48 h post-injection, whereas renal ultrastructural recovery of crucian carp in the 50 mu g MC-LReq. kg(-1) dose group was evident at 168 h post-injection. Our ultrastructural observation suggests that the membranous structure is the main action site of MCs in the kidney, among which mitochondria damage in the tubules is clearly an early, and presumably a critically important effect of MCs. The increases in blood urea nitrogen (BUN) and creatinine (CR) in both dose groups further revealed severe impairment occurred in the kidney of crucian carp. (C) 2013 Elsevier Ltd. All rights reserved.; An acute toxicological experiment was designed to characterize the sequence of renal ultrastructural changes with accumulated MCs in crucian carp injected intraperitoneally (i.p.) with extracted microcystins (mainly MC-RR and -LR) at two doses, 50 and 200 mu g MC-LReq. kg(-1) body weight. Quantitative and qualitative determinations of MCs in the kidney were conducted by HPLC and LC-MS, respectively. MC-RR content in kidney of crucian carp showed a time dose-dependent increase within 48 h post-injection, followed by a sharp decline afterward, while no MC-LR in kidney was detectable throughout the experiment. Ultrastructural changes in the kidney of crucian carp progressed with increasing accumulated MCs and exposure times within 48 h post-injection, whereas renal ultrastructural recovery of crucian carp in the 50 mu g MC-LReq. kg(-1) dose group was evident at 168 h post-injection. Our ultrastructural observation suggests that the membranous structure is the main action site of MCs in the kidney, among which mitochondria damage in the tubules is clearly an early, and presumably a critically important effect of MCs. The increases in blood urea nitrogen (BUN) and creatinine (CR) in both dose groups further revealed severe impairment occurred in the kidney of crucian carp. (C) 2013 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordMicrocystins Nephrotoxic Intraperitoneal Injection Crucian Carp Ultrastructural Changes
Department[Li, Li ; Zhang, Xuezhen] Huazhong Agr Univ, Coll Fisheries, Minist Agr, Key Lab Freshwater Anim Breeding, Wuhan 430070, Peoples R China ; [Xie, Ping] Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China ; [Lei, Hehua] Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan 430072, Peoples R China
DOI10.1016/j.toxicon.2013.03.022
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [31100378]; Fundamental Research Funds for the Central Universities [2010QC022] ; National Natural Science Foundation of China [31100378]; Fundamental Research Funds for the Central Universities [2010QC022] ; National Natural Science Foundation of China [31100378]; Fundamental Research Funds for the Central Universities [2010QC022] ; National Natural Science Foundation of China [31100378]; Fundamental Research Funds for the Central Universities [2010QC022]
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy ; Toxicology
WOS SubjectPharmacology & Pharmacy ; Toxicology
WOS IDWOS:000321537200008
WOS KeywordPHYTOPLANKTIVOROUS SILVER CARP ; TOXIC CYANOBACTERIAL BLOOMS ; DIFFERENT TROPHIC LEVELS ; FISH OREOCHROMIS SP. ; LABORATORY CONDITIONS ; BIGHEAD CARP ; IN-SITU ; BIOCHEMICAL-CHARACTERIZATION ; TISSUE DISTRIBUTION ; OXIDATIVE STRESS
Funding OrganizationNational Natural Science Foundation of China [31100378]; Fundamental Research Funds for the Central Universities [2010QC022] ; National Natural Science Foundation of China [31100378]; Fundamental Research Funds for the Central Universities [2010QC022] ; National Natural Science Foundation of China [31100378]; Fundamental Research Funds for the Central Universities [2010QC022] ; National Natural Science Foundation of China [31100378]; Fundamental Research Funds for the Central Universities [2010QC022]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19551
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorLi, L (reprint author), Huazhong Agr Univ, Coll Fisheries, Minist Agr, Key Lab Freshwater Anim Breeding, Wuhan 430070, Peoples R China.
Affiliation1.Huazhong Agr Univ, Coll Fisheries, Minist Agr, Key Lab Freshwater Anim Breeding, Wuhan 430070, Peoples R China
2.Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China
3.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Li, Li,Xie, Ping,Lei, Hehua,et al. Renal accumulation and effects of intraperitoneal injection of extracted microcystins in omnivorous crucian carp (Carassius auratus)[J]. TOXICON,2013,70:62-69.
APA Li, Li,Xie, Ping,Lei, Hehua,Zhang, Xuezhen,&Li, L .(2013).Renal accumulation and effects of intraperitoneal injection of extracted microcystins in omnivorous crucian carp (Carassius auratus).TOXICON,70,62-69.
MLA Li, Li,et al."Renal accumulation and effects of intraperitoneal injection of extracted microcystins in omnivorous crucian carp (Carassius auratus)".TOXICON 70(2013):62-69.
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