| MOTU analysis of ichthyoplankton biodiversity in the upper Yangtze River, China | |
| Cheng, F.1,2; Li, W.2; Wu, Q. J.1; Murphy, B. R.3; Xie, S. G.1,4; Xie, SG (reprint author), Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organisms, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China. | |
| 2013-08-01 | |
| Source Publication | JOURNAL OF APPLIED ICHTHYOLOGY
 |
| ISSN | 0175-8659 |
| Volume | 29Issue:4Pages:872-876 |
| Abstract | Identification of ichthyoplankton is difficult because fish during early life stages often lack stable morphological characteristics; such difficulty in species identification can be a major hindrance in conducting ichthyoplankton surveys for fish biodiversity investigations. Here, we evaluated the feasibility of a molecular operational taxonomic unit (MOTU) approach for ichthyoplankton investigations, and describe fish biodiversity in the Jinshajiang section of the upper Yangtze River, China. The MOTUs were established by grouping specimens diverging less than 1.00% Kimura two-parameter (K2P) distance units from their nearest neighbor within the same MOTU, based on previous work on between-species divergences of the mitochondrial cytochrome C oxidase subunit I (COI) gene. Taxonomic assignment of the MOTUs was performed by comparing the MOTU sequences with the COI sequences of taxonomic species. Sixty-eight MOTUs were inferred from 818 COI sequences of ichthyoplankton in the Jinshajiang river section. Among those, one MOTU was composed of two identified taxonomic species, and each of the other MOTUs was linked to a single, identified taxonomic species. Only 26 MOTUs were successfully identified to taxonomic species due to the limited reference database. Our results demonstrate that the MOTU approach can be applied successfully for analyzing biodiversity and identifying species of freshwater ichthyoplankton. Compared with previous ichthyoplankton investigations the richness of ichthyoplankton was very high. High diversity of ichthyoplankton noted in our study suggests that the Jinshajiang section should be an important target for fish biodiversity conservation in the Yangtze River.; Identification of ichthyoplankton is difficult because fish during early life stages often lack stable morphological characteristics; such difficulty in species identification can be a major hindrance in conducting ichthyoplankton surveys for fish biodiversity investigations. Here, we evaluated the feasibility of a molecular operational taxonomic unit (MOTU) approach for ichthyoplankton investigations, and describe fish biodiversity in the Jinshajiang section of the upper Yangtze River, China. The MOTUs were established by grouping specimens diverging less than 1.00% Kimura two-parameter (K2P) distance units from their nearest neighbor within the same MOTU, based on previous work on between-species divergences of the mitochondrial cytochrome C oxidase subunit I (COI) gene. Taxonomic assignment of the MOTUs was performed by comparing the MOTU sequences with the COI sequences of taxonomic species. Sixty-eight MOTUs were inferred from 818 COI sequences of ichthyoplankton in the Jinshajiang river section. Among those, one MOTU was composed of two identified taxonomic species, and each of the other MOTUs was linked to a single, identified taxonomic species. Only 26 MOTUs were successfully identified to taxonomic species due to the limited reference database. Our results demonstrate that the MOTU approach can be applied successfully for analyzing biodiversity and identifying species of freshwater ichthyoplankton. Compared with previous ichthyoplankton investigations the richness of ichthyoplankton was very high. High diversity of ichthyoplankton noted in our study suggests that the Jinshajiang section should be an important target for fish biodiversity conservation in the Yangtze River. |
| Subtype | Article |
| Keyword | Molecular-identification Dna Barcodes Fish Eggs Conservation Patterns Larvae Strategies Diversity Sturgeon |
| Department | [Cheng, F. ; Wu, Q. J. ; Xie, S. G.] Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China ; [Cheng, F. ; Li, W.] Chinese Acad Sci, Key Lab Aquat Bot & Watershed Ecol, Wuhan Bot Garden, Wuhan 430072, Hubei, Peoples R China ; [Murphy, B. R.] Virginia Polytech Inst & State Univ, Dept Fish & Wildlife Conservat, Blacksburg, VA 24061 USA ; [Xie, S. G.] Chinese Acad Sci, Key Lab Sustainable Fisheries & Environm Protect, Huaian Res Ctr, Inst Hydrobiol, Huaian, Jiangsu, Peoples R China |
| DOI | 10.1111/jai.12207 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | National Basic Research Program of China (Program 973) [2010CB429005]; Postdoctoral Natural Science Foundation of China [20100480924]; State Key Laboratory of Freshwater Ecology and Biotechnology [Y15C01]; Nature Conservancy; Chinese Academy of Sciences [2010T2S25]; One-Hundred Talents Program of the Chinese Academy of Sciences ; National Basic Research Program of China (Program 973) [2010CB429005]; Postdoctoral Natural Science Foundation of China [20100480924]; State Key Laboratory of Freshwater Ecology and Biotechnology [Y15C01]; Nature Conservancy; Chinese Academy of Sciences [2010T2S25]; One-Hundred Talents Program of the Chinese Academy of Sciences ; National Basic Research Program of China (Program 973) [2010CB429005]; Postdoctoral Natural Science Foundation of China [20100480924]; State Key Laboratory of Freshwater Ecology and Biotechnology [Y15C01]; Nature Conservancy; Chinese Academy of Sciences [2010T2S25]; One-Hundred Talents Program of the Chinese Academy of Sciences ; National Basic Research Program of China (Program 973) [2010CB429005]; Postdoctoral Natural Science Foundation of China [20100480924]; State Key Laboratory of Freshwater Ecology and Biotechnology [Y15C01]; Nature Conservancy; Chinese Academy of Sciences [2010T2S25]; One-Hundred Talents Program of the Chinese Academy of Sciences |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Fisheries ; Marine & Freshwater Biology |
| WOS Subject | Fisheries ; Marine & Freshwater Biology |
| WOS ID | WOS:000321436500029 |
| WOS Keyword | MOLECULAR-IDENTIFICATION ; DNA BARCODES ; FISH EGGS ; CONSERVATION ; PATTERNS ; LARVAE ; STRATEGIES ; DIVERSITY ; STURGEON |
| Funding Organization | National Basic Research Program of China (Program 973) [2010CB429005]; Postdoctoral Natural Science Foundation of China [20100480924]; State Key Laboratory of Freshwater Ecology and Biotechnology [Y15C01]; Nature Conservancy; Chinese Academy of Sciences [2010T2S25]; One-Hundred Talents Program of the Chinese Academy of Sciences ; National Basic Research Program of China (Program 973) [2010CB429005]; Postdoctoral Natural Science Foundation of China [20100480924]; State Key Laboratory of Freshwater Ecology and Biotechnology [Y15C01]; Nature Conservancy; Chinese Academy of Sciences [2010T2S25]; One-Hundred Talents Program of the Chinese Academy of Sciences ; National Basic Research Program of China (Program 973) [2010CB429005]; Postdoctoral Natural Science Foundation of China [20100480924]; State Key Laboratory of Freshwater Ecology and Biotechnology [Y15C01]; Nature Conservancy; Chinese Academy of Sciences [2010T2S25]; One-Hundred Talents Program of the Chinese Academy of Sciences ; National Basic Research Program of China (Program 973) [2010CB429005]; Postdoctoral Natural Science Foundation of China [20100480924]; State Key Laboratory of Freshwater Ecology and Biotechnology [Y15C01]; Nature Conservancy; Chinese Academy of Sciences [2010T2S25]; One-Hundred Talents Program of the Chinese Academy of Sciences |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/19550 |
| Collection | 淡水生态学研究中心_期刊论文 |
| Corresponding Author | Xie, SG (reprint author), Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organisms, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China. |
| Affiliation | 1.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Key Lab Aquat Bot & Watershed Ecol, Wuhan Bot Garden, Wuhan 430072, Hubei, Peoples R China 3.Virginia Polytech Inst & State Univ, Dept Fish & Wildlife Conservat, Blacksburg, VA 24061 USA 4.Chinese Acad Sci, Key Lab Sustainable Fisheries & Environm Protect, Huaian Res Ctr, Inst Hydrobiol, Huaian, Jiangsu, Peoples R China |
| Recommended Citation GB/T 7714 | Cheng, F.,Li, W.,Wu, Q. J.,et al. MOTU analysis of ichthyoplankton biodiversity in the upper Yangtze River, China[J]. JOURNAL OF APPLIED ICHTHYOLOGY,2013,29(4):872-876. |
| APA | Cheng, F.,Li, W.,Wu, Q. J.,Murphy, B. R.,Xie, S. G.,&Xie, SG .(2013).MOTU analysis of ichthyoplankton biodiversity in the upper Yangtze River, China.JOURNAL OF APPLIED ICHTHYOLOGY,29(4),872-876. |
| MLA | Cheng, F.,et al."MOTU analysis of ichthyoplankton biodiversity in the upper Yangtze River, China".JOURNAL OF APPLIED ICHTHYOLOGY 29.4(2013):872-876. |
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