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MOTU analysis of ichthyoplankton biodiversity in the upper Yangtze River, China
Cheng, F.1,2; Li, W.2; Wu, Q. J.1; Murphy, B. R.3; Xie, S. G.1,4; Xie, SG (reprint author), Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organisms, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China.
2013-08-01
Source PublicationJOURNAL OF APPLIED ICHTHYOLOGY
ISSN0175-8659
Volume29Issue:4Pages:872-876
AbstractIdentification of ichthyoplankton is difficult because fish during early life stages often lack stable morphological characteristics; such difficulty in species identification can be a major hindrance in conducting ichthyoplankton surveys for fish biodiversity investigations. Here, we evaluated the feasibility of a molecular operational taxonomic unit (MOTU) approach for ichthyoplankton investigations, and describe fish biodiversity in the Jinshajiang section of the upper Yangtze River, China. The MOTUs were established by grouping specimens diverging less than 1.00% Kimura two-parameter (K2P) distance units from their nearest neighbor within the same MOTU, based on previous work on between-species divergences of the mitochondrial cytochrome C oxidase subunit I (COI) gene. Taxonomic assignment of the MOTUs was performed by comparing the MOTU sequences with the COI sequences of taxonomic species. Sixty-eight MOTUs were inferred from 818 COI sequences of ichthyoplankton in the Jinshajiang river section. Among those, one MOTU was composed of two identified taxonomic species, and each of the other MOTUs was linked to a single, identified taxonomic species. Only 26 MOTUs were successfully identified to taxonomic species due to the limited reference database. Our results demonstrate that the MOTU approach can be applied successfully for analyzing biodiversity and identifying species of freshwater ichthyoplankton. Compared with previous ichthyoplankton investigations the richness of ichthyoplankton was very high. High diversity of ichthyoplankton noted in our study suggests that the Jinshajiang section should be an important target for fish biodiversity conservation in the Yangtze River.; Identification of ichthyoplankton is difficult because fish during early life stages often lack stable morphological characteristics; such difficulty in species identification can be a major hindrance in conducting ichthyoplankton surveys for fish biodiversity investigations. Here, we evaluated the feasibility of a molecular operational taxonomic unit (MOTU) approach for ichthyoplankton investigations, and describe fish biodiversity in the Jinshajiang section of the upper Yangtze River, China. The MOTUs were established by grouping specimens diverging less than 1.00% Kimura two-parameter (K2P) distance units from their nearest neighbor within the same MOTU, based on previous work on between-species divergences of the mitochondrial cytochrome C oxidase subunit I (COI) gene. Taxonomic assignment of the MOTUs was performed by comparing the MOTU sequences with the COI sequences of taxonomic species. Sixty-eight MOTUs were inferred from 818 COI sequences of ichthyoplankton in the Jinshajiang river section. Among those, one MOTU was composed of two identified taxonomic species, and each of the other MOTUs was linked to a single, identified taxonomic species. Only 26 MOTUs were successfully identified to taxonomic species due to the limited reference database. Our results demonstrate that the MOTU approach can be applied successfully for analyzing biodiversity and identifying species of freshwater ichthyoplankton. Compared with previous ichthyoplankton investigations the richness of ichthyoplankton was very high. High diversity of ichthyoplankton noted in our study suggests that the Jinshajiang section should be an important target for fish biodiversity conservation in the Yangtze River.
SubtypeArticle
KeywordMolecular-identification Dna Barcodes Fish Eggs Conservation Patterns Larvae Strategies Diversity Sturgeon
Department[Cheng, F. ; Wu, Q. J. ; Xie, S. G.] Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China ; [Cheng, F. ; Li, W.] Chinese Acad Sci, Key Lab Aquat Bot & Watershed Ecol, Wuhan Bot Garden, Wuhan 430072, Hubei, Peoples R China ; [Murphy, B. R.] Virginia Polytech Inst & State Univ, Dept Fish & Wildlife Conservat, Blacksburg, VA 24061 USA ; [Xie, S. G.] Chinese Acad Sci, Key Lab Sustainable Fisheries & Environm Protect, Huaian Res Ctr, Inst Hydrobiol, Huaian, Jiangsu, Peoples R China
DOI10.1111/jai.12207
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Basic Research Program of China (Program 973) [2010CB429005]; Postdoctoral Natural Science Foundation of China [20100480924]; State Key Laboratory of Freshwater Ecology and Biotechnology [Y15C01]; Nature Conservancy; Chinese Academy of Sciences [2010T2S25]; One-Hundred Talents Program of the Chinese Academy of Sciences ; National Basic Research Program of China (Program 973) [2010CB429005]; Postdoctoral Natural Science Foundation of China [20100480924]; State Key Laboratory of Freshwater Ecology and Biotechnology [Y15C01]; Nature Conservancy; Chinese Academy of Sciences [2010T2S25]; One-Hundred Talents Program of the Chinese Academy of Sciences ; National Basic Research Program of China (Program 973) [2010CB429005]; Postdoctoral Natural Science Foundation of China [20100480924]; State Key Laboratory of Freshwater Ecology and Biotechnology [Y15C01]; Nature Conservancy; Chinese Academy of Sciences [2010T2S25]; One-Hundred Talents Program of the Chinese Academy of Sciences ; National Basic Research Program of China (Program 973) [2010CB429005]; Postdoctoral Natural Science Foundation of China [20100480924]; State Key Laboratory of Freshwater Ecology and Biotechnology [Y15C01]; Nature Conservancy; Chinese Academy of Sciences [2010T2S25]; One-Hundred Talents Program of the Chinese Academy of Sciences
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000321436500029
WOS KeywordMOLECULAR-IDENTIFICATION ; DNA BARCODES ; FISH EGGS ; CONSERVATION ; PATTERNS ; LARVAE ; STRATEGIES ; DIVERSITY ; STURGEON
Funding OrganizationNational Basic Research Program of China (Program 973) [2010CB429005]; Postdoctoral Natural Science Foundation of China [20100480924]; State Key Laboratory of Freshwater Ecology and Biotechnology [Y15C01]; Nature Conservancy; Chinese Academy of Sciences [2010T2S25]; One-Hundred Talents Program of the Chinese Academy of Sciences ; National Basic Research Program of China (Program 973) [2010CB429005]; Postdoctoral Natural Science Foundation of China [20100480924]; State Key Laboratory of Freshwater Ecology and Biotechnology [Y15C01]; Nature Conservancy; Chinese Academy of Sciences [2010T2S25]; One-Hundred Talents Program of the Chinese Academy of Sciences ; National Basic Research Program of China (Program 973) [2010CB429005]; Postdoctoral Natural Science Foundation of China [20100480924]; State Key Laboratory of Freshwater Ecology and Biotechnology [Y15C01]; Nature Conservancy; Chinese Academy of Sciences [2010T2S25]; One-Hundred Talents Program of the Chinese Academy of Sciences ; National Basic Research Program of China (Program 973) [2010CB429005]; Postdoctoral Natural Science Foundation of China [20100480924]; State Key Laboratory of Freshwater Ecology and Biotechnology [Y15C01]; Nature Conservancy; Chinese Academy of Sciences [2010T2S25]; One-Hundred Talents Program of the Chinese Academy of Sciences
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Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19550
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorXie, SG (reprint author), Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organisms, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China.
Affiliation1.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Key Lab Aquat Bot & Watershed Ecol, Wuhan Bot Garden, Wuhan 430072, Hubei, Peoples R China
3.Virginia Polytech Inst & State Univ, Dept Fish & Wildlife Conservat, Blacksburg, VA 24061 USA
4.Chinese Acad Sci, Key Lab Sustainable Fisheries & Environm Protect, Huaian Res Ctr, Inst Hydrobiol, Huaian, Jiangsu, Peoples R China
Recommended Citation
GB/T 7714
Cheng, F.,Li, W.,Wu, Q. J.,et al. MOTU analysis of ichthyoplankton biodiversity in the upper Yangtze River, China[J]. JOURNAL OF APPLIED ICHTHYOLOGY,2013,29(4):872-876.
APA Cheng, F.,Li, W.,Wu, Q. J.,Murphy, B. R.,Xie, S. G.,&Xie, SG .(2013).MOTU analysis of ichthyoplankton biodiversity in the upper Yangtze River, China.JOURNAL OF APPLIED ICHTHYOLOGY,29(4),872-876.
MLA Cheng, F.,et al."MOTU analysis of ichthyoplankton biodiversity in the upper Yangtze River, China".JOURNAL OF APPLIED ICHTHYOLOGY 29.4(2013):872-876.
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