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Why mammals more susceptible to the hepatotoxic microcystins than fish: evidences from plasma and albumin protein binding through equilibrium dialysis
Zhang, Wei1,2; Liang, Gaodao1; Wu, Laiyan1; Tuo, Xun1; Wang, Wenjing1; Chen, Jun1; Xie, Ping1; Xie, P (reprint author), Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China.
2013-08-01
Source PublicationECOTOXICOLOGY
ISSN0963-9292
Volume22Issue:6Pages:1012-1019
AbstractTo elucidate the interspecies variation of susceptibility to microcystins (MCs), fresh plasma and purified albumin from six kinds of mammals and fish were used in toxins-substances binding test. Protein contents in the test plasma were analyzed and the binding characteristics to MCs were compared. Two kinds of widely observed MCs, microcystin-LR (MC-LR) and microcystin-RR (MC-RR) were tested and data were collected through the method of equilibrium dialysis. It was found that total plasma protein and albumin content in mammals were nearly two times and four times higher than that in fish, respectively. In the test range of 0-100 mu g/mL, binding rates of fish plasma to MCs were considered significant lower (p < 0.01) than that of mammals. And human plasma demonstrated the highest binding rate in mammals. In all the test species, plasma protein binding rates of MC-RR were significantly higher than MC-LR (p < 0.01). Besides, binding profiles of albumin were acquired under the protein content of 0.67 mg/mL. Human serum albumin demonstrated the highest affinity to MCs throughout the six species and differences among the other five species were considered not significant (p > 0.05). From the view of protein binding, it is concluded that both the variation of plasma protein composition and albumin binding characteristic could influence the existing form of MCs in circulation, change MCs utilization, alter MCs half-life and further contribute to the difference of susceptibility between mammals and fish.; To elucidate the interspecies variation of susceptibility to microcystins (MCs), fresh plasma and purified albumin from six kinds of mammals and fish were used in toxins-substances binding test. Protein contents in the test plasma were analyzed and the binding characteristics to MCs were compared. Two kinds of widely observed MCs, microcystin-LR (MC-LR) and microcystin-RR (MC-RR) were tested and data were collected through the method of equilibrium dialysis. It was found that total plasma protein and albumin content in mammals were nearly two times and four times higher than that in fish, respectively. In the test range of 0-100 mu g/mL, binding rates of fish plasma to MCs were considered significant lower (p < 0.01) than that of mammals. And human plasma demonstrated the highest binding rate in mammals. In all the test species, plasma protein binding rates of MC-RR were significantly higher than MC-LR (p < 0.01). Besides, binding profiles of albumin were acquired under the protein content of 0.67 mg/mL. Human serum albumin demonstrated the highest affinity to MCs throughout the six species and differences among the other five species were considered not significant (p > 0.05). From the view of protein binding, it is concluded that both the variation of plasma protein composition and albumin binding characteristic could influence the existing form of MCs in circulation, change MCs utilization, alter MCs half-life and further contribute to the difference of susceptibility between mammals and fish.
SubtypeArticle
KeywordMicrocystins Plasma Protein Albumin Equilibrium Dialysis
Department[Zhang, Wei; Liang, Gaodao; Wu, Laiyan; Tuo, Xun; Wang, Wenjing; Chen, Jun; Xie, Ping] Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Zhang, Wei] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
DOI10.1007/s10646-013-1086-5
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationState Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ07] ; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ07] ; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ07] ; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ07]
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Toxicology
WOS SubjectEcology ; Environmental Sciences ; Toxicology
WOS IDWOS:000322166100005
WOS KeywordCYCLIC PEPTIDE TOXIN ; HUMAN SERUM-ALBUMIN ; CYPRINUS-CARPIO L ; BLUE-GREEN-ALGA ; INTRAPERITONEAL INJECTION ; TISSUE DISTRIBUTION ; ATLANTIC SALMON ; OCHRATOXIN-A ; IN-VIVO ; LR
Funding OrganizationState Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ07] ; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ07] ; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ07] ; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ07]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19549
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorXie, P (reprint author), Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Wei,Liang, Gaodao,Wu, Laiyan,et al. Why mammals more susceptible to the hepatotoxic microcystins than fish: evidences from plasma and albumin protein binding through equilibrium dialysis[J]. ECOTOXICOLOGY,2013,22(6):1012-1019.
APA Zhang, Wei.,Liang, Gaodao.,Wu, Laiyan.,Tuo, Xun.,Wang, Wenjing.,...&Xie, P .(2013).Why mammals more susceptible to the hepatotoxic microcystins than fish: evidences from plasma and albumin protein binding through equilibrium dialysis.ECOTOXICOLOGY,22(6),1012-1019.
MLA Zhang, Wei,et al."Why mammals more susceptible to the hepatotoxic microcystins than fish: evidences from plasma and albumin protein binding through equilibrium dialysis".ECOTOXICOLOGY 22.6(2013):1012-1019.
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