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Toxic effects of microcystin-LR on the HepG2 cell line under hypoxic and normoxic conditions
Zhang, Xin1,2; Xie, Ping2; Zhang, Xuezhen1; Zhou, Wenshan1,2; Zhao, Sujuan2; Zhao, Yanyan2; Cai, Yan2; Xie, P (reprint author), Inst Hydrobiol, Donghu South Rd 7, Wuhan 430072, Peoples R China.
2013-10-01
Source PublicationJOURNAL OF APPLIED TOXICOLOGY
ISSN0260-437X
Volume33Issue:10Pages:1180-1186
AbstractMicrocystins (MCs) are highly liver-specific and evidenced as a liver tumor promoter. Oxidative stress is one of the most important toxicity mechanisms of MCs, which is tightly related to oxygen concentration. The effects of MCs on animals and cell lines in normoxia and the mechanisms have been well studied, but such effects in different oxygen conditions were still unclear. The aim of the present study was to explore the cellular response of the human hepatocellular carcinoma cell line (HepG2) to MC-LR exposure under hypoxic (1% O-2) and normoxic (21% O-2) conditions. We examined cell viability, mitochondrial membrane potential (MMP), superoxide dismutase (SOD) activity and gene expression posttoxin exposure. Cell viability was increased by MC-LR in normoxia although decreased in hypoxia. MC-LR markedly induced MMP loss under hypoxic condition but only slightly MMP loss under normoxic condition. SOD activity was significantly induced by MC-LR in hypoxia, indicating prolonged oxidative stress. Inhibitory apoptosis protein (c-IAP2) was significantly up-regulated by MC-LR under normoxic condition, suggesting that c-IAP2 played an important role in the promotion of cell proliferation by MC-LR. These results indicate that MC-LR promotes cell proliferation under normoxic condition whereas induces cell apoptosis under hypoxic condition. Copyright (C) 2012 John Wiley & Sons, Ltd.; Microcystins (MCs) are highly liver-specific and evidenced as a liver tumor promoter. Oxidative stress is one of the most important toxicity mechanisms of MCs, which is tightly related to oxygen concentration. The effects of MCs on animals and cell lines in normoxia and the mechanisms have been well studied, but such effects in different oxygen conditions were still unclear. The aim of the present study was to explore the cellular response of the human hepatocellular carcinoma cell line (HepG2) to MC-LR exposure under hypoxic (1% O-2) and normoxic (21% O-2) conditions. We examined cell viability, mitochondrial membrane potential (MMP), superoxide dismutase (SOD) activity and gene expression posttoxin exposure. Cell viability was increased by MC-LR in normoxia although decreased in hypoxia. MC-LR markedly induced MMP loss under hypoxic condition but only slightly MMP loss under normoxic condition. SOD activity was significantly induced by MC-LR in hypoxia, indicating prolonged oxidative stress. Inhibitory apoptosis protein (c-IAP2) was significantly up-regulated by MC-LR under normoxic condition, suggesting that c-IAP2 played an important role in the promotion of cell proliferation by MC-LR. These results indicate that MC-LR promotes cell proliferation under normoxic condition whereas induces cell apoptosis under hypoxic condition. Copyright (C) 2012 John Wiley & Sons, Ltd.
SubtypeArticle
KeywordMc-lr Hypoxia Normoxia Oxidative Stress Apoptosis Proliferation
Department[Zhang, Xin ; Zhang, Xuezhen ; Zhou, Wenshan] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China ; [Zhang, Xin ; Xie, Ping ; Zhou, Wenshan ; Zhao, Sujuan ; Zhao, Yanyan ; Cai, Yan] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
DOI10.1002/jat.2749
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationChina National Fundamental Fund of Personnel Training [J0730649] ; China National Fundamental Fund of Personnel Training [J0730649] ; China National Fundamental Fund of Personnel Training [J0730649] ; China National Fundamental Fund of Personnel Training [J0730649]
Indexed BySCI
Language英语
WOS Research AreaToxicology
WOS SubjectToxicology
WOS IDWOS:000322331900015
WOS KeywordINDUCED APOPTOSIS ; OXIDATIVE STRESS ; RAT HEPATOCYTES ; IN-VIVO ; OXYGEN ; LIVER ; CYANOBACTERIA ; HEPATOTOXIN ; TRANSPORT ; PATHWAY
Funding OrganizationChina National Fundamental Fund of Personnel Training [J0730649] ; China National Fundamental Fund of Personnel Training [J0730649] ; China National Fundamental Fund of Personnel Training [J0730649] ; China National Fundamental Fund of Personnel Training [J0730649]
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19544
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorXie, P (reprint author), Inst Hydrobiol, Donghu South Rd 7, Wuhan 430072, Peoples R China.
Affiliation1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Xin,Xie, Ping,Zhang, Xuezhen,et al. Toxic effects of microcystin-LR on the HepG2 cell line under hypoxic and normoxic conditions[J]. JOURNAL OF APPLIED TOXICOLOGY,2013,33(10):1180-1186.
APA Zhang, Xin.,Xie, Ping.,Zhang, Xuezhen.,Zhou, Wenshan.,Zhao, Sujuan.,...&Xie, P .(2013).Toxic effects of microcystin-LR on the HepG2 cell line under hypoxic and normoxic conditions.JOURNAL OF APPLIED TOXICOLOGY,33(10),1180-1186.
MLA Zhang, Xin,et al."Toxic effects of microcystin-LR on the HepG2 cell line under hypoxic and normoxic conditions".JOURNAL OF APPLIED TOXICOLOGY 33.10(2013):1180-1186.
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