| Toxic effects of microcystin-LR on the HepG2 cell line under hypoxic and normoxic conditions | |
| Zhang, Xin1,2; Xie, Ping2; Zhang, Xuezhen1; Zhou, Wenshan1,2; Zhao, Sujuan2; Zhao, Yanyan2; Cai, Yan2; Xie, P (reprint author), Inst Hydrobiol, Donghu South Rd 7, Wuhan 430072, Peoples R China. | |
| 2013-10-01 | |
| Source Publication | JOURNAL OF APPLIED TOXICOLOGY
 |
| ISSN | 0260-437X |
| Volume | 33Issue:10Pages:1180-1186 |
| Abstract | Microcystins (MCs) are highly liver-specific and evidenced as a liver tumor promoter. Oxidative stress is one of the most important toxicity mechanisms of MCs, which is tightly related to oxygen concentration. The effects of MCs on animals and cell lines in normoxia and the mechanisms have been well studied, but such effects in different oxygen conditions were still unclear. The aim of the present study was to explore the cellular response of the human hepatocellular carcinoma cell line (HepG2) to MC-LR exposure under hypoxic (1% O-2) and normoxic (21% O-2) conditions. We examined cell viability, mitochondrial membrane potential (MMP), superoxide dismutase (SOD) activity and gene expression posttoxin exposure. Cell viability was increased by MC-LR in normoxia although decreased in hypoxia. MC-LR markedly induced MMP loss under hypoxic condition but only slightly MMP loss under normoxic condition. SOD activity was significantly induced by MC-LR in hypoxia, indicating prolonged oxidative stress. Inhibitory apoptosis protein (c-IAP2) was significantly up-regulated by MC-LR under normoxic condition, suggesting that c-IAP2 played an important role in the promotion of cell proliferation by MC-LR. These results indicate that MC-LR promotes cell proliferation under normoxic condition whereas induces cell apoptosis under hypoxic condition. Copyright (C) 2012 John Wiley & Sons, Ltd.; Microcystins (MCs) are highly liver-specific and evidenced as a liver tumor promoter. Oxidative stress is one of the most important toxicity mechanisms of MCs, which is tightly related to oxygen concentration. The effects of MCs on animals and cell lines in normoxia and the mechanisms have been well studied, but such effects in different oxygen conditions were still unclear. The aim of the present study was to explore the cellular response of the human hepatocellular carcinoma cell line (HepG2) to MC-LR exposure under hypoxic (1% O-2) and normoxic (21% O-2) conditions. We examined cell viability, mitochondrial membrane potential (MMP), superoxide dismutase (SOD) activity and gene expression posttoxin exposure. Cell viability was increased by MC-LR in normoxia although decreased in hypoxia. MC-LR markedly induced MMP loss under hypoxic condition but only slightly MMP loss under normoxic condition. SOD activity was significantly induced by MC-LR in hypoxia, indicating prolonged oxidative stress. Inhibitory apoptosis protein (c-IAP2) was significantly up-regulated by MC-LR under normoxic condition, suggesting that c-IAP2 played an important role in the promotion of cell proliferation by MC-LR. These results indicate that MC-LR promotes cell proliferation under normoxic condition whereas induces cell apoptosis under hypoxic condition. Copyright (C) 2012 John Wiley & Sons, Ltd. |
| Subtype | Article |
| Keyword | Mc-lr Hypoxia Normoxia Oxidative Stress Apoptosis Proliferation |
| Department | [Zhang, Xin ; Zhang, Xuezhen ; Zhou, Wenshan] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China ; [Zhang, Xin ; Xie, Ping ; Zhou, Wenshan ; Zhao, Sujuan ; Zhao, Yanyan ; Cai, Yan] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China |
| DOI | 10.1002/jat.2749 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | China National Fundamental Fund of Personnel Training [J0730649] ; China National Fundamental Fund of Personnel Training [J0730649] ; China National Fundamental Fund of Personnel Training [J0730649] ; China National Fundamental Fund of Personnel Training [J0730649] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Toxicology |
| WOS Subject | Toxicology |
| WOS ID | WOS:000322331900015 |
| WOS Keyword | INDUCED APOPTOSIS ; OXIDATIVE STRESS ; RAT HEPATOCYTES ; IN-VIVO ; OXYGEN ; LIVER ; CYANOBACTERIA ; HEPATOTOXIN ; TRANSPORT ; PATHWAY |
| Funding Organization | China National Fundamental Fund of Personnel Training [J0730649] ; China National Fundamental Fund of Personnel Training [J0730649] ; China National Fundamental Fund of Personnel Training [J0730649] ; China National Fundamental Fund of Personnel Training [J0730649] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/19544 |
| Collection | 淡水生态学研究中心_期刊论文 |
| Corresponding Author | Xie, P (reprint author), Inst Hydrobiol, Donghu South Rd 7, Wuhan 430072, Peoples R China. |
| Affiliation | 1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Zhang, Xin,Xie, Ping,Zhang, Xuezhen,et al. Toxic effects of microcystin-LR on the HepG2 cell line under hypoxic and normoxic conditions[J]. JOURNAL OF APPLIED TOXICOLOGY,2013,33(10):1180-1186. |
| APA | Zhang, Xin.,Xie, Ping.,Zhang, Xuezhen.,Zhou, Wenshan.,Zhao, Sujuan.,...&Xie, P .(2013).Toxic effects of microcystin-LR on the HepG2 cell line under hypoxic and normoxic conditions.JOURNAL OF APPLIED TOXICOLOGY,33(10),1180-1186. |
| MLA | Zhang, Xin,et al."Toxic effects of microcystin-LR on the HepG2 cell line under hypoxic and normoxic conditions".JOURNAL OF APPLIED TOXICOLOGY 33.10(2013):1180-1186. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Toxic effects of mic（321KB） | 开放获取 | CC BY-NC-SA | View Application Full Text | |||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment