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题名: Toxic effects of microcystin-LR on the HepG2 cell line under hypoxic and normoxic conditions
作者: Zhang, Xin1, 2; Xie, Ping2; Zhang, Xuezhen1; Zhou, Wenshan1, 2; Zhao, Sujuan2; Zhao, Yanyan2; Cai, Yan2
通讯作者: Xie, P (reprint author), Inst Hydrobiol, Donghu South Rd 7, Wuhan 430072, Peoples R China.
关键词: MC-LR ; hypoxia ; normoxia ; oxidative stress ; apoptosis ; proliferation
刊名: JOURNAL OF APPLIED TOXICOLOGY
发表日期: 2013-10-01
DOI: 10.1002/jat.2749
卷: 33, 期:10, 页:1180-1186
收录类别: SCI
文章类型: Article
部门归属: [Zhang, Xin ; Zhang, Xuezhen ; Zhou, Wenshan] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China ; [Zhang, Xin ; Xie, Ping ; Zhou, Wenshan ; Zhao, Sujuan ; Zhao, Yanyan ; Cai, Yan] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: China National Fundamental Fund of Personnel Training [J0730649]
类目[WOS]: Toxicology
研究领域[WOS]: Toxicology
摘要: Microcystins (MCs) are highly liver-specific and evidenced as a liver tumor promoter. Oxidative stress is one of the most important toxicity mechanisms of MCs, which is tightly related to oxygen concentration. The effects of MCs on animals and cell lines in normoxia and the mechanisms have been well studied, but such effects in different oxygen conditions were still unclear. The aim of the present study was to explore the cellular response of the human hepatocellular carcinoma cell line (HepG2) to MC-LR exposure under hypoxic (1% O-2) and normoxic (21% O-2) conditions. We examined cell viability, mitochondrial membrane potential (MMP), superoxide dismutase (SOD) activity and gene expression posttoxin exposure. Cell viability was increased by MC-LR in normoxia although decreased in hypoxia. MC-LR markedly induced MMP loss under hypoxic condition but only slightly MMP loss under normoxic condition. SOD activity was significantly induced by MC-LR in hypoxia, indicating prolonged oxidative stress. Inhibitory apoptosis protein (c-IAP2) was significantly up-regulated by MC-LR under normoxic condition, suggesting that c-IAP2 played an important role in the promotion of cell proliferation by MC-LR. These results indicate that MC-LR promotes cell proliferation under normoxic condition whereas induces cell apoptosis under hypoxic condition. Copyright (C) 2012 John Wiley & Sons, Ltd.
英文摘要: Microcystins (MCs) are highly liver-specific and evidenced as a liver tumor promoter. Oxidative stress is one of the most important toxicity mechanisms of MCs, which is tightly related to oxygen concentration. The effects of MCs on animals and cell lines in normoxia and the mechanisms have been well studied, but such effects in different oxygen conditions were still unclear. The aim of the present study was to explore the cellular response of the human hepatocellular carcinoma cell line (HepG2) to MC-LR exposure under hypoxic (1% O-2) and normoxic (21% O-2) conditions. We examined cell viability, mitochondrial membrane potential (MMP), superoxide dismutase (SOD) activity and gene expression posttoxin exposure. Cell viability was increased by MC-LR in normoxia although decreased in hypoxia. MC-LR markedly induced MMP loss under hypoxic condition but only slightly MMP loss under normoxic condition. SOD activity was significantly induced by MC-LR in hypoxia, indicating prolonged oxidative stress. Inhibitory apoptosis protein (c-IAP2) was significantly up-regulated by MC-LR under normoxic condition, suggesting that c-IAP2 played an important role in the promotion of cell proliferation by MC-LR. These results indicate that MC-LR promotes cell proliferation under normoxic condition whereas induces cell apoptosis under hypoxic condition. Copyright (C) 2012 John Wiley & Sons, Ltd.
关键词[WOS]: INDUCED APOPTOSIS ; OXIDATIVE STRESS ; RAT HEPATOCYTES ; IN-VIVO ; OXYGEN ; LIVER ; CYANOBACTERIA ; HEPATOTOXIN ; TRANSPORT ; PATHWAY
语种: 英语
WOS记录号: WOS:000322331900015
ISSN号: 0260-437X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19544
Appears in Collections:淡水生态学研究中心_期刊论文

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作者单位: 1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China

Recommended Citation:
Zhang, Xin; Xie, Ping; Zhang, Xuezhen; Zhou, Wenshan; Zhao, Sujuan; Zhao, Yanyan; Cai, Yan.Toxic effects of microcystin-LR on the HepG2 cell line under hypoxic and normoxic conditions,JOURNAL OF APPLIED TOXICOLOGY,2013,33(10):1180-1186
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