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题名: Identification and Expression Profiles of IL-8 in Bighead Carp (Aristichthys nobilis) in Response to Microcystin-LR
作者: Li, Huiying1; Cai, Yan1; Xie, Ping1; Li, Guangyu1; Hao, Le1; Xiong, Qian1
通讯作者: Xie, P (reprint author), Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China.
关键词: INTERLEUKIN-8 GENE-EXPRESSION ; PRO-INFLAMMATORY CYTOKINES ; TROUT ONCORHYNCHUS-MYKISS ; 3'-UNTRANSLATED REGION ; REAL-TIME ; IN-VIVO ; CHEMOKINES ; IL-1-BETA ; CLONING ; MACROPHAGES
刊名: ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
发表日期: 2013-10-01
DOI: 10.1007/s00244-013-9910-8
卷: 65, 期:3, 页:537-545
收录类别: SCI
文章类型: Article
部门归属: [Li, Huiying ; Cai, Yan ; Xie, Ping ; Li, Guangyu ; Hao, Le ; Xiong, Qian] Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ01]
类目[WOS]: Environmental Sciences ; Toxicology
研究领域[WOS]: Environmental Sciences & Ecology ; Toxicology
摘要: Microcystin-LR (MCLR) is a widespread cyanotoxin and has immunotoxicity to animals, including fish. Chemokines are considered to play important roles in inflammatory response induced by MCLR. In this study, we cloned the full-length cDNA of interleukin-8 (IL-8) from bighead carp (Aristichthys nobilis) for the first time. The full-length IL-8 cDNA was 552 bp and contained a 297-bp open-reading frame that encoded for a 98-amino acid protein. The deduced IL-8 protein had a typical aspartic acid (D)-leucine (L)-arginine (R) and a CXC motif at the N-terminal, which were conserved in most fish species. Phylogenetic analysis showed that bighead carp IL-8 protein was grouped in the teleost IL-8 lineage 2. Under normal conditions, the expression of IL-8 is constitutive and weak in all tested tissues. However, MCLR treatment could significantly increase the transcription of IL-8 in bighead carp in a temporal- and dose-dependent pattern. The present study will help us to understand more about the evolution of IL-8 and its function in the MCLR induced proinflammatory response in bighead carp.
英文摘要: Microcystin-LR (MCLR) is a widespread cyanotoxin and has immunotoxicity to animals, including fish. Chemokines are considered to play important roles in inflammatory response induced by MCLR. In this study, we cloned the full-length cDNA of interleukin-8 (IL-8) from bighead carp (Aristichthys nobilis) for the first time. The full-length IL-8 cDNA was 552 bp and contained a 297-bp open-reading frame that encoded for a 98-amino acid protein. The deduced IL-8 protein had a typical aspartic acid (D)-leucine (L)-arginine (R) and a CXC motif at the N-terminal, which were conserved in most fish species. Phylogenetic analysis showed that bighead carp IL-8 protein was grouped in the teleost IL-8 lineage 2. Under normal conditions, the expression of IL-8 is constitutive and weak in all tested tissues. However, MCLR treatment could significantly increase the transcription of IL-8 in bighead carp in a temporal- and dose-dependent pattern. The present study will help us to understand more about the evolution of IL-8 and its function in the MCLR induced proinflammatory response in bighead carp.
关键词[WOS]: INTERLEUKIN-8 GENE-EXPRESSION ; PRO-INFLAMMATORY CYTOKINES ; TROUT ONCORHYNCHUS-MYKISS ; 3'-UNTRANSLATED REGION ; REAL-TIME ; IN-VIVO ; CHEMOKINES ; IL-1-BETA ; CLONING ; MACROPHAGES
语种: 英语
WOS记录号: WOS:000324074200017
ISSN号: 0090-4341
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19543
Appears in Collections:淡水生态学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China

Recommended Citation:
Li, Huiying; Cai, Yan; Xie, Ping; Li, Guangyu; Hao, Le; Xiong, Qian.Identification and Expression Profiles of IL-8 in Bighead Carp (Aristichthys nobilis) in Response to Microcystin-LR,ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY,2013,65(3):537-545
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