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Identification and Expression Profiles of IL-8 in Bighead Carp (Aristichthys nobilis) in Response to Microcystin-LR
Li, Huiying; Cai, Yan; Xie, Ping; Li, Guangyu; Hao, Le; Xiong, Qian; Xie, P (reprint author), Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China.
2013-10-01
Source PublicationARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
ISSN0090-4341
Volume65Issue:3Pages:537-545
AbstractMicrocystin-LR (MCLR) is a widespread cyanotoxin and has immunotoxicity to animals, including fish. Chemokines are considered to play important roles in inflammatory response induced by MCLR. In this study, we cloned the full-length cDNA of interleukin-8 (IL-8) from bighead carp (Aristichthys nobilis) for the first time. The full-length IL-8 cDNA was 552 bp and contained a 297-bp open-reading frame that encoded for a 98-amino acid protein. The deduced IL-8 protein had a typical aspartic acid (D)-leucine (L)-arginine (R) and a CXC motif at the N-terminal, which were conserved in most fish species. Phylogenetic analysis showed that bighead carp IL-8 protein was grouped in the teleost IL-8 lineage 2. Under normal conditions, the expression of IL-8 is constitutive and weak in all tested tissues. However, MCLR treatment could significantly increase the transcription of IL-8 in bighead carp in a temporal- and dose-dependent pattern. The present study will help us to understand more about the evolution of IL-8 and its function in the MCLR induced proinflammatory response in bighead carp.; Microcystin-LR (MCLR) is a widespread cyanotoxin and has immunotoxicity to animals, including fish. Chemokines are considered to play important roles in inflammatory response induced by MCLR. In this study, we cloned the full-length cDNA of interleukin-8 (IL-8) from bighead carp (Aristichthys nobilis) for the first time. The full-length IL-8 cDNA was 552 bp and contained a 297-bp open-reading frame that encoded for a 98-amino acid protein. The deduced IL-8 protein had a typical aspartic acid (D)-leucine (L)-arginine (R) and a CXC motif at the N-terminal, which were conserved in most fish species. Phylogenetic analysis showed that bighead carp IL-8 protein was grouped in the teleost IL-8 lineage 2. Under normal conditions, the expression of IL-8 is constitutive and weak in all tested tissues. However, MCLR treatment could significantly increase the transcription of IL-8 in bighead carp in a temporal- and dose-dependent pattern. The present study will help us to understand more about the evolution of IL-8 and its function in the MCLR induced proinflammatory response in bighead carp.
SubtypeArticle
KeywordInterleukin-8 Gene-expression Pro-inflammatory Cytokines Trout Oncorhynchus-mykiss 3'-untranslated Region Real-time In-vivo Chemokines Il-1-beta Cloning Macrophages
Department[Li, Huiying ; Cai, Yan ; Xie, Ping ; Li, Guangyu ; Hao, Le ; Xiong, Qian] Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China
DOI10.1007/s00244-013-9910-8
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ01] ; National Natural Science Foundation of China [31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ01] ; National Natural Science Foundation of China [31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ01] ; National Natural Science Foundation of China [31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ01]
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Toxicology
WOS SubjectEnvironmental Sciences ; Toxicology
WOS IDWOS:000324074200017
WOS KeywordINTERLEUKIN-8 GENE-EXPRESSION ; PRO-INFLAMMATORY CYTOKINES ; TROUT ONCORHYNCHUS-MYKISS ; 3'-UNTRANSLATED REGION ; REAL-TIME ; IN-VIVO ; CHEMOKINES ; IL-1-BETA ; CLONING ; MACROPHAGES
Funding OrganizationNational Natural Science Foundation of China [31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ01] ; National Natural Science Foundation of China [31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ01] ; National Natural Science Foundation of China [31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ01] ; National Natural Science Foundation of China [31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ01]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19543
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorXie, P (reprint author), Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China.
AffiliationChinese Acad Sci, Donghu Expt Stn Lake Ecosyst, Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Li, Huiying,Cai, Yan,Xie, Ping,et al. Identification and Expression Profiles of IL-8 in Bighead Carp (Aristichthys nobilis) in Response to Microcystin-LR[J]. ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY,2013,65(3):537-545.
APA Li, Huiying.,Cai, Yan.,Xie, Ping.,Li, Guangyu.,Hao, Le.,...&Xie, P .(2013).Identification and Expression Profiles of IL-8 in Bighead Carp (Aristichthys nobilis) in Response to Microcystin-LR.ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY,65(3),537-545.
MLA Li, Huiying,et al."Identification and Expression Profiles of IL-8 in Bighead Carp (Aristichthys nobilis) in Response to Microcystin-LR".ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY 65.3(2013):537-545.
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