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题名: Sex Identification of Four Penguin Species Using Locus-Specific PCR
作者: Zhang, Peijun1, 2, 3; Han, Jiabo1; Liu, Quansheng4; Zhang, Junxin4; Zhang, Xianfeng2, 3
通讯作者: Zhang, XF (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
关键词: Aptenodytes patagonicus ; Eudyptes chrysocome ; Pygoscelis papua ; Spheniscus magellanicus ; CHD1
刊名: ZOO BIOLOGY
发表日期: 2013-05-01
DOI: 10.1002/zoo.21005
卷: 32, 期:3, 页:257-261
收录类别: SCI
文章类型: Article
部门归属: [Zhang, Peijun ; Han, Jiabo] Liaoning Ocean & Fisheries Sci Res Inst, Dept Conservat Biol Endangered Anim, Dalian, Peoples R China ; [Zhang, Peijun ; Zhang, Xianfeng] Chinese Acad Sci, Inst Hydrobiol, Res Grp Conservat Biol Aquat Anim, Wuhan 430072, Peoples R China ; [Zhang, Peijun ; Zhang, Xianfeng] Chinese Acad Sci, Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China ; [Liu, Quansheng ; Zhang, Junxin] Hangzhou Polar Ocean Pk, Ctr Aquat Anim, Hangzhou, Zhejiang, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: Ocean Park Conservation Foundation, Hong Kong [IHB/CN/2007065]
类目[WOS]: Veterinary Sciences ; Zoology
研究领域[WOS]: Veterinary Sciences ; Zoology
摘要: Traditional methods for sex identification are not applicable to sexually monomorphic species, leading to difficulties in the management of their breeding programs. To identify sex in sexually monomorphic birds, molecular methods have been established. Two established primer pairs (2550F/2718R and p8/p2) amplify the CHD1 gene region from both the Z and W chromosomes. Here, we evaluated the use of these primers for sex identification in four sexually monomorphic penguin species: king penguins (Aptenodytes patagonicus), rockhopper penguins (Eudyptes chrysocome), gentoo penguins (Pygoscelis papua), and Magellanic penguins (Spheniscus magellanicus). For all species except rockhopper penguins, primer pair 2550F/2718R resulted in two distinct CHD1Z and CHD1W PCR bands, allowing for sex identification. For rockhopper penguins, only primer pair p8/p2 yielded different CHD1Z and CHD1W bands, which were faint and similar in size making them difficult to distinguish. As a result, we designed a new primer pair (PL/PR) that efficiently determined the gender of individuals from all four penguin species. Sequencing of the PCR products confirmed that they were from the CHD1 gene region. Primer pair PL/PR can be evaluated for use in sexing other penguin species, which will be crucial for the management of new penguin breeding programs. Zoo Biol 32:257-261, 2013. (c) 2012 Wiley Periodicals, Inc.
英文摘要: Traditional methods for sex identification are not applicable to sexually monomorphic species, leading to difficulties in the management of their breeding programs. To identify sex in sexually monomorphic birds, molecular methods have been established. Two established primer pairs (2550F/2718R and p8/p2) amplify the CHD1 gene region from both the Z and W chromosomes. Here, we evaluated the use of these primers for sex identification in four sexually monomorphic penguin species: king penguins (Aptenodytes patagonicus), rockhopper penguins (Eudyptes chrysocome), gentoo penguins (Pygoscelis papua), and Magellanic penguins (Spheniscus magellanicus). For all species except rockhopper penguins, primer pair 2550F/2718R resulted in two distinct CHD1Z and CHD1W PCR bands, allowing for sex identification. For rockhopper penguins, only primer pair p8/p2 yielded different CHD1Z and CHD1W bands, which were faint and similar in size making them difficult to distinguish. As a result, we designed a new primer pair (PL/PR) that efficiently determined the gender of individuals from all four penguin species. Sequencing of the PCR products confirmed that they were from the CHD1 gene region. Primer pair PL/PR can be evaluated for use in sexing other penguin species, which will be crucial for the management of new penguin breeding programs. Zoo Biol 32:257-261, 2013. (c) 2012 Wiley Periodicals, Inc.
关键词[WOS]: BIRDS ; CHROMOSOME ; SEQUENCE ; TOOLS
语种: 英语
WOS记录号: WOS:000320139100004
ISSN号: 0733-3188
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19538
Appears in Collections:水生生物多样性与资源保护研究中心_期刊论文

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作者单位: 1.Liaoning Ocean & Fisheries Sci Res Inst, Dept Conservat Biol Endangered Anim, Dalian, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Res Grp Conservat Biol Aquat Anim, Wuhan 430072, Peoples R China
3.Chinese Acad Sci, Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China
4.Hangzhou Polar Ocean Pk, Ctr Aquat Anim, Hangzhou, Zhejiang, Peoples R China

Recommended Citation:
Zhang, Peijun; Han, Jiabo; Liu, Quansheng; Zhang, Junxin; Zhang, Xianfeng.Sex Identification of Four Penguin Species Using Locus-Specific PCR,ZOO BIOLOGY,2013,32(3):257-261
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