IHB OpenIR  > 水生生物多样性与资源保护研究中心  > 期刊论文
Sex Identification of Four Penguin Species Using Locus-Specific PCR
Zhang, Peijun1,2,3; Han, Jiabo1; Liu, Quansheng4; Zhang, Junxin4; Zhang, Xianfeng2,3; Zhang, XF (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
2013-05-01
Source PublicationZOO BIOLOGY
ISSN0733-3188
Volume32Issue:3Pages:257-261
AbstractTraditional methods for sex identification are not applicable to sexually monomorphic species, leading to difficulties in the management of their breeding programs. To identify sex in sexually monomorphic birds, molecular methods have been established. Two established primer pairs (2550F/2718R and p8/p2) amplify the CHD1 gene region from both the Z and W chromosomes. Here, we evaluated the use of these primers for sex identification in four sexually monomorphic penguin species: king penguins (Aptenodytes patagonicus), rockhopper penguins (Eudyptes chrysocome), gentoo penguins (Pygoscelis papua), and Magellanic penguins (Spheniscus magellanicus). For all species except rockhopper penguins, primer pair 2550F/2718R resulted in two distinct CHD1Z and CHD1W PCR bands, allowing for sex identification. For rockhopper penguins, only primer pair p8/p2 yielded different CHD1Z and CHD1W bands, which were faint and similar in size making them difficult to distinguish. As a result, we designed a new primer pair (PL/PR) that efficiently determined the gender of individuals from all four penguin species. Sequencing of the PCR products confirmed that they were from the CHD1 gene region. Primer pair PL/PR can be evaluated for use in sexing other penguin species, which will be crucial for the management of new penguin breeding programs. Zoo Biol 32:257-261, 2013. (c) 2012 Wiley Periodicals, Inc.; Traditional methods for sex identification are not applicable to sexually monomorphic species, leading to difficulties in the management of their breeding programs. To identify sex in sexually monomorphic birds, molecular methods have been established. Two established primer pairs (2550F/2718R and p8/p2) amplify the CHD1 gene region from both the Z and W chromosomes. Here, we evaluated the use of these primers for sex identification in four sexually monomorphic penguin species: king penguins (Aptenodytes patagonicus), rockhopper penguins (Eudyptes chrysocome), gentoo penguins (Pygoscelis papua), and Magellanic penguins (Spheniscus magellanicus). For all species except rockhopper penguins, primer pair 2550F/2718R resulted in two distinct CHD1Z and CHD1W PCR bands, allowing for sex identification. For rockhopper penguins, only primer pair p8/p2 yielded different CHD1Z and CHD1W bands, which were faint and similar in size making them difficult to distinguish. As a result, we designed a new primer pair (PL/PR) that efficiently determined the gender of individuals from all four penguin species. Sequencing of the PCR products confirmed that they were from the CHD1 gene region. Primer pair PL/PR can be evaluated for use in sexing other penguin species, which will be crucial for the management of new penguin breeding programs. Zoo Biol 32:257-261, 2013. (c) 2012 Wiley Periodicals, Inc.
SubtypeArticle
KeywordAptenodytes Patagonicus Eudyptes Chrysocome Pygoscelis Papua Spheniscus Magellanicus Chd1
Department[Zhang, Peijun ; Han, Jiabo] Liaoning Ocean & Fisheries Sci Res Inst, Dept Conservat Biol Endangered Anim, Dalian, Peoples R China ; [Zhang, Peijun ; Zhang, Xianfeng] Chinese Acad Sci, Inst Hydrobiol, Res Grp Conservat Biol Aquat Anim, Wuhan 430072, Peoples R China ; [Zhang, Peijun ; Zhang, Xianfeng] Chinese Acad Sci, Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China ; [Liu, Quansheng ; Zhang, Junxin] Hangzhou Polar Ocean Pk, Ctr Aquat Anim, Hangzhou, Zhejiang, Peoples R China
DOI10.1002/zoo.21005
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationOcean Park Conservation Foundation, Hong Kong [IHB/CN/2007065] ; Ocean Park Conservation Foundation, Hong Kong [IHB/CN/2007065] ; Ocean Park Conservation Foundation, Hong Kong [IHB/CN/2007065] ; Ocean Park Conservation Foundation, Hong Kong [IHB/CN/2007065]
Indexed BySCI
Language英语
WOS Research AreaVeterinary Sciences ; Zoology
WOS SubjectVeterinary Sciences ; Zoology
WOS IDWOS:000320139100004
WOS KeywordBIRDS ; CHROMOSOME ; SEQUENCE ; TOOLS
Funding OrganizationOcean Park Conservation Foundation, Hong Kong [IHB/CN/2007065] ; Ocean Park Conservation Foundation, Hong Kong [IHB/CN/2007065] ; Ocean Park Conservation Foundation, Hong Kong [IHB/CN/2007065] ; Ocean Park Conservation Foundation, Hong Kong [IHB/CN/2007065]
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19538
Collection水生生物多样性与资源保护研究中心_期刊论文
Corresponding AuthorZhang, XF (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
Affiliation1.Liaoning Ocean & Fisheries Sci Res Inst, Dept Conservat Biol Endangered Anim, Dalian, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Res Grp Conservat Biol Aquat Anim, Wuhan 430072, Peoples R China
3.Chinese Acad Sci, Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China
4.Hangzhou Polar Ocean Pk, Ctr Aquat Anim, Hangzhou, Zhejiang, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Peijun,Han, Jiabo,Liu, Quansheng,et al. Sex Identification of Four Penguin Species Using Locus-Specific PCR[J]. ZOO BIOLOGY,2013,32(3):257-261.
APA Zhang, Peijun,Han, Jiabo,Liu, Quansheng,Zhang, Junxin,Zhang, Xianfeng,&Zhang, XF .(2013).Sex Identification of Four Penguin Species Using Locus-Specific PCR.ZOO BIOLOGY,32(3),257-261.
MLA Zhang, Peijun,et al."Sex Identification of Four Penguin Species Using Locus-Specific PCR".ZOO BIOLOGY 32.3(2013):257-261.
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