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Analysis of Flagellar Protein Ubiquitination
Long, H; Huang, KY
Source PublicationCILIA, PT A -- Methods in Enzymology
ContributorMarshall, WF
Publication PlaceINC, 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 USA
AbstractFlagella/cilia are conserved organelles existing in unicellular protists and multicellular animals, where they perform essential motile and sensory functions. Their assembly and disassembly are coordinated with the cell cycle, and recent evidence shows that posttranslational modifications such as phosphorylation, methylation, and ubiquitination are involved in these two processes, perhaps through interacting with intraflagellar transport (IFT), a specialized intracellular transport that is required for the assembly and maintenance of flagella/cilia. In this chapter, we summarize the components of the ubiquitination system published in proteomic databases of flagella/cilia. Furthermore, we describe procedures to analyze the ubiquitin-conjugating system in Chlamydomonas flagella and to analyze flagellar protein ubiquitination during flagellar shortening and the mating process. These results and tools will be valuable for the characterization of substrates of ubiquitination and their roles in flagellar disassembly and in regulating signal transduction pathways in flagella/cilia.
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Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type专著章节/文集论文
Recommended Citation
GB/T 7714
Long, H,Huang, KY. Analysis of Flagellar Protein Ubiquitination. CILIA, PT A -- Methods in Enzymology. INC, 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 USA:ELSEVIER ACADEMIC PRESS,2013.
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