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MBL-Mediated Opsonophagocytosis of Candida albicans by Human Neutrophils Is Coupled with Intracellular Dectin-1-Triggered ROS Production
Li, Dongsheng1; Dong, Bilin1; Tong, Zhongsheng1; Wang, Qinning2; Liu, Weihuang3; Wang, Yan4; Liu, Wei4; Chen, Jinbo1; Xu, Li1; Chen, Liuqing1; Duan, Yiqun1; Chen, LQ (reprint author), 1 Hosp Wuhan, Dept Dermatol, Ctr Infect Skin Dis, Wuhan, Peoples R China.
2012-12-11
Source PublicationPLOS ONE
ISSN1932-6203
Volume7Issue:12Pages:e50589
AbstractMannan-binding lectin (MBL), a lectin homologous to C1q, greatly facilitates C3/C4-mediated opsonophagocytosis of Candida albicans (C. albicans) by human neutrophils, and has the capacity to bind to CR1 (CD35) expressed on circulating neutrophils. The intracellular pool of neutrophil Dectin-1 plays a critical role in stimulating the reactive oxygen species (ROS) generation through recognition of beta-1,3-glucan component of phagocytized zymosan or yeasts. However, little is known about whether MBL can mediate the opsonophagocytosis of Candida albicans by neutrophils independent of complement activation, and whether MBL-mediated opsonophagocytosis influence the intracellular expression of Dectin-1 and ROS production. Here we showed that the inhibited phagocytic efficiency of neutrophils as a result of blockage of Dectin-1 was compensated by exogenous MBL alone in a dose-dependent manner. Furthermore, the expressions of Dectin-1 at mRNA and intracellular protein levels were significantly up-regulated in neutrophils stimulated by MBL-pre-incubated C. albicans, while the expression of surface Dectin-1 remained almost unchanged. Nevertheless, the stimulated ROS production in neutrophils was partly and irreversibly inhibited by blockage of Dectin-1 in the presence of exogenous MBL. Confocal microscopy examination showed that intracellular Dectin-1 was recruited and co-distributed with ROS on the surface of some phagocytized yeasts. The beta-1,3-glucanase digestion test further suggested that the specific recognition and binding site of human Dectin-1 is just the beta-1,3-glucan moiety on the cell wall of C. albicans. These data demonstrate that MBL has an ability to mediate the opsonophagocytosis of Candida albicans by human neutrophils independent of complement activation, which is coupled with intracellular Dectin-1-triggered ROS production.; Mannan-binding lectin (MBL), a lectin homologous to C1q, greatly facilitates C3/C4-mediated opsonophagocytosis of Candida albicans (C. albicans) by human neutrophils, and has the capacity to bind to CR1 (CD35) expressed on circulating neutrophils. The intracellular pool of neutrophil Dectin-1 plays a critical role in stimulating the reactive oxygen species (ROS) generation through recognition of beta-1,3-glucan component of phagocytized zymosan or yeasts. However, little is known about whether MBL can mediate the opsonophagocytosis of Candida albicans by neutrophils independent of complement activation, and whether MBL-mediated opsonophagocytosis influence the intracellular expression of Dectin-1 and ROS production. Here we showed that the inhibited phagocytic efficiency of neutrophils as a result of blockage of Dectin-1 was compensated by exogenous MBL alone in a dose-dependent manner. Furthermore, the expressions of Dectin-1 at mRNA and intracellular protein levels were significantly up-regulated in neutrophils stimulated by MBL-pre-incubated C. albicans, while the expression of surface Dectin-1 remained almost unchanged. Nevertheless, the stimulated ROS production in neutrophils was partly and irreversibly inhibited by blockage of Dectin-1 in the presence of exogenous MBL. Confocal microscopy examination showed that intracellular Dectin-1 was recruited and co-distributed with ROS on the surface of some phagocytized yeasts. The beta-1,3-glucanase digestion test further suggested that the specific recognition and binding site of human Dectin-1 is just the beta-1,3-glucan moiety on the cell wall of C. albicans. These data demonstrate that MBL has an ability to mediate the opsonophagocytosis of Candida albicans by human neutrophils independent of complement activation, which is coupled with intracellular Dectin-1-triggered ROS production.
SubtypeArticle
KeywordMannan-binding Lectin Fungal-infections Dectin-1 Recognition Activation Receptor Mannose Yeast Cells
Department[Li, Dongsheng; Dong, Bilin; Tong, Zhongsheng; Chen, Jinbo; Xu, Li; Chen, Liuqing; Duan, Yiqun] 1 Hosp Wuhan, Dept Dermatol, Ctr Infect Skin Dis, Wuhan, Peoples R China; [Wang, Qinning] Univ Sydney, Westmead Hosp, Ctr Infect Dis & Microbiol Lab Serv, Westmead, NSW 2145, Australia; [Liu, Weihuang] Wuhan Univ, Med Res Ctr, Wuhan 430072, Peoples R China; [Wang, Yan; Liu, Wei] Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
DOI10.1371/journal.pone.0050589
WOS HeadingsScience & Technology
Funding OrganizationWuhan Health Bureau [[2011] 99]; Hubei Health Department, China [JX3A25] ; Wuhan Health Bureau [[2011] 99]; Hubei Health Department, China [JX3A25] ; Wuhan Health Bureau [[2011] 99]; Hubei Health Department, China [JX3A25] ; Wuhan Health Bureau [[2011] 99]; Hubei Health Department, China [JX3A25]
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000312290800020
WOS KeywordMANNAN-BINDING LECTIN ; FUNGAL-INFECTIONS ; DECTIN-1 ; RECOGNITION ; ACTIVATION ; RECEPTOR ; MANNOSE ; YEAST ; CELLS
Funding OrganizationWuhan Health Bureau [[2011] 99]; Hubei Health Department, China [JX3A25] ; Wuhan Health Bureau [[2011] 99]; Hubei Health Department, China [JX3A25] ; Wuhan Health Bureau [[2011] 99]; Hubei Health Department, China [JX3A25] ; Wuhan Health Bureau [[2011] 99]; Hubei Health Department, China [JX3A25]
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Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19362
Collection分析测试中心_期刊论文
Corresponding AuthorChen, LQ (reprint author), 1 Hosp Wuhan, Dept Dermatol, Ctr Infect Skin Dis, Wuhan, Peoples R China.
Affiliation1.1 Hosp Wuhan, Dept Dermatol, Ctr Infect Skin Dis, Wuhan, Peoples R China
2.Univ Sydney, Westmead Hosp, Ctr Infect Dis & Microbiol Lab Serv, Westmead, NSW 2145, Australia
3.Wuhan Univ, Med Res Ctr, Wuhan 430072, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Li, Dongsheng,Dong, Bilin,Tong, Zhongsheng,et al. MBL-Mediated Opsonophagocytosis of Candida albicans by Human Neutrophils Is Coupled with Intracellular Dectin-1-Triggered ROS Production[J]. PLOS ONE,2012,7(12):e50589.
APA Li, Dongsheng.,Dong, Bilin.,Tong, Zhongsheng.,Wang, Qinning.,Liu, Weihuang.,...&Chen, LQ .(2012).MBL-Mediated Opsonophagocytosis of Candida albicans by Human Neutrophils Is Coupled with Intracellular Dectin-1-Triggered ROS Production.PLOS ONE,7(12),e50589.
MLA Li, Dongsheng,et al."MBL-Mediated Opsonophagocytosis of Candida albicans by Human Neutrophils Is Coupled with Intracellular Dectin-1-Triggered ROS Production".PLOS ONE 7.12(2012):e50589.
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