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题名: MBL-Mediated Opsonophagocytosis of Candida albicans by Human Neutrophils Is Coupled with Intracellular Dectin-1-Triggered ROS Production
作者: Li, Dongsheng1; Dong, Bilin1; Tong, Zhongsheng1; Wang, Qinning2; Liu, Weihuang3; Wang, Yan4; Liu, Wei4; Chen, Jinbo1; Xu, Li1; Chen, Liuqing1; Duan, Yiqun1
通讯作者: Chen, LQ (reprint author), 1 Hosp Wuhan, Dept Dermatol, Ctr Infect Skin Dis, Wuhan, Peoples R China.
关键词: MANNAN-BINDING LECTIN ; FUNGAL-INFECTIONS ; DECTIN-1 ; RECOGNITION ; ACTIVATION ; RECEPTOR ; MANNOSE ; YEAST ; CELLS
刊名: PLOS ONE
发表日期: 2012-12-11
DOI: 10.1371/journal.pone.0050589
卷: 7, 期:12, 页:e50589
收录类别: SCI
文章类型: Article
部门归属: [Li, Dongsheng; Dong, Bilin; Tong, Zhongsheng; Chen, Jinbo; Xu, Li; Chen, Liuqing; Duan, Yiqun] 1 Hosp Wuhan, Dept Dermatol, Ctr Infect Skin Dis, Wuhan, Peoples R China; [Wang, Qinning] Univ Sydney, Westmead Hosp, Ctr Infect Dis & Microbiol Lab Serv, Westmead, NSW 2145, Australia; [Liu, Weihuang] Wuhan Univ, Med Res Ctr, Wuhan 430072, Peoples R China; [Wang, Yan; Liu, Wei] Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
WOS标题词: Science & Technology
资助者: Wuhan Health Bureau [[2011] 99]; Hubei Health Department, China [JX3A25]
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
摘要: Mannan-binding lectin (MBL), a lectin homologous to C1q, greatly facilitates C3/C4-mediated opsonophagocytosis of Candida albicans (C. albicans) by human neutrophils, and has the capacity to bind to CR1 (CD35) expressed on circulating neutrophils. The intracellular pool of neutrophil Dectin-1 plays a critical role in stimulating the reactive oxygen species (ROS) generation through recognition of beta-1,3-glucan component of phagocytized zymosan or yeasts. However, little is known about whether MBL can mediate the opsonophagocytosis of Candida albicans by neutrophils independent of complement activation, and whether MBL-mediated opsonophagocytosis influence the intracellular expression of Dectin-1 and ROS production. Here we showed that the inhibited phagocytic efficiency of neutrophils as a result of blockage of Dectin-1 was compensated by exogenous MBL alone in a dose-dependent manner. Furthermore, the expressions of Dectin-1 at mRNA and intracellular protein levels were significantly up-regulated in neutrophils stimulated by MBL-pre-incubated C. albicans, while the expression of surface Dectin-1 remained almost unchanged. Nevertheless, the stimulated ROS production in neutrophils was partly and irreversibly inhibited by blockage of Dectin-1 in the presence of exogenous MBL. Confocal microscopy examination showed that intracellular Dectin-1 was recruited and co-distributed with ROS on the surface of some phagocytized yeasts. The beta-1,3-glucanase digestion test further suggested that the specific recognition and binding site of human Dectin-1 is just the beta-1,3-glucan moiety on the cell wall of C. albicans. These data demonstrate that MBL has an ability to mediate the opsonophagocytosis of Candida albicans by human neutrophils independent of complement activation, which is coupled with intracellular Dectin-1-triggered ROS production.
英文摘要: Mannan-binding lectin (MBL), a lectin homologous to C1q, greatly facilitates C3/C4-mediated opsonophagocytosis of Candida albicans (C. albicans) by human neutrophils, and has the capacity to bind to CR1 (CD35) expressed on circulating neutrophils. The intracellular pool of neutrophil Dectin-1 plays a critical role in stimulating the reactive oxygen species (ROS) generation through recognition of beta-1,3-glucan component of phagocytized zymosan or yeasts. However, little is known about whether MBL can mediate the opsonophagocytosis of Candida albicans by neutrophils independent of complement activation, and whether MBL-mediated opsonophagocytosis influence the intracellular expression of Dectin-1 and ROS production. Here we showed that the inhibited phagocytic efficiency of neutrophils as a result of blockage of Dectin-1 was compensated by exogenous MBL alone in a dose-dependent manner. Furthermore, the expressions of Dectin-1 at mRNA and intracellular protein levels were significantly up-regulated in neutrophils stimulated by MBL-pre-incubated C. albicans, while the expression of surface Dectin-1 remained almost unchanged. Nevertheless, the stimulated ROS production in neutrophils was partly and irreversibly inhibited by blockage of Dectin-1 in the presence of exogenous MBL. Confocal microscopy examination showed that intracellular Dectin-1 was recruited and co-distributed with ROS on the surface of some phagocytized yeasts. The beta-1,3-glucanase digestion test further suggested that the specific recognition and binding site of human Dectin-1 is just the beta-1,3-glucan moiety on the cell wall of C. albicans. These data demonstrate that MBL has an ability to mediate the opsonophagocytosis of Candida albicans by human neutrophils independent of complement activation, which is coupled with intracellular Dectin-1-triggered ROS production.
关键词[WOS]: MANNAN-BINDING LECTIN ; FUNGAL-INFECTIONS ; DECTIN-1 ; RECOGNITION ; ACTIVATION ; RECEPTOR ; MANNOSE ; YEAST ; CELLS
语种: 英语
WOS记录号: WOS:000312290800020
ISSN号: 1932-6203
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19362
Appears in Collections:分析测试中心_期刊论文

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作者单位: 1.1 Hosp Wuhan, Dept Dermatol, Ctr Infect Skin Dis, Wuhan, Peoples R China
2.Univ Sydney, Westmead Hosp, Ctr Infect Dis & Microbiol Lab Serv, Westmead, NSW 2145, Australia
3.Wuhan Univ, Med Res Ctr, Wuhan 430072, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
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