IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
The Roles of follistatin 1 in Regulation of Zebrafish Fecundity and Sexual Differentiation
Jiang, Nan1,2; Jin, Xia1; He, Jiangyan1; Yin, Zhan1; Yin, Z (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China.
2012-09-01
Source PublicationBIOLOGY OF REPRODUCTION
ISSN0006-3363
Volume87Issue:3
AbstractFollistatin 1 (Fst1) is a binding protein of activin and some other members of the transforming growth factor beta superfamily. It plays a key role in the regulation of gonadal function in vertebrates. An oocyte-specific promoter, derived from the zona pellucida 3 (zp3) gene, was used to create transgenic fst1 zebrafish (Danio rerio). Three independent oocyte-specific overexpression fst1 transgenic zebrafish lines were generated. Decreased levels of phosphorylated Smad3 were observed in ovarian tissues in fst1 transgenic fish compared with those from their control female siblings. Analyses on the numbers of mature eggs also indicated the attenuated oocyte maturation in the fst1 transgenic fish and in the females administered recombinant human Fst protein. Remarkably, when raised in the same tank with their control siblings, a significantly larger proportion of the fst1 transgenic population developed as males compared to the controls. Moreover, assessing the levels of active caspase 3 in gonadal tissues at 30 days postfertilization, we observed increased levels of apoptosis in the transitioning gonads of the transgenic fish compared to nontransgenic control siblings. Our results demonstrate that zebrafish Fst1 not only acts as an inhibitory binding protein of activin in the regulation of oocyte maturation in adult females but also plays a potential role in the masculinization of juveniles. Overall, the present study contributes to our understanding of the paracrine roles of fst1 as well as normal oocyte maturation and gonadal differentiation.; Follistatin 1 (Fst1) is a binding protein of activin and some other members of the transforming growth factor beta superfamily. It plays a key role in the regulation of gonadal function in vertebrates. An oocyte-specific promoter, derived from the zona pellucida 3 (zp3) gene, was used to create transgenic fst1 zebrafish (Danio rerio). Three independent oocyte-specific overexpression fst1 transgenic zebrafish lines were generated. Decreased levels of phosphorylated Smad3 were observed in ovarian tissues in fst1 transgenic fish compared with those from their control female siblings. Analyses on the numbers of mature eggs also indicated the attenuated oocyte maturation in the fst1 transgenic fish and in the females administered recombinant human Fst protein. Remarkably, when raised in the same tank with their control siblings, a significantly larger proportion of the fst1 transgenic population developed as males compared to the controls. Moreover, assessing the levels of active caspase 3 in gonadal tissues at 30 days postfertilization, we observed increased levels of apoptosis in the transitioning gonads of the transgenic fish compared to nontransgenic control siblings. Our results demonstrate that zebrafish Fst1 not only acts as an inhibitory binding protein of activin in the regulation of oocyte maturation in adult females but also plays a potential role in the masculinization of juveniles. Overall, the present study contributes to our understanding of the paracrine roles of fst1 as well as normal oocyte maturation and gonadal differentiation.
SubtypeArticle
KeywordFish Reproduction Follistatin Fst1 Masculinization Oocyte Maturation Ovulation Sex Differentiation Transitioning Gonad Zebrafish
Department[Jiang, Nan; Jin, Xia; He, Jiangyan; Yin, Zhan] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China; [Jiang, Nan] Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
DOI10.1095/biolreprod.112.099689
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Basic Research Program of China (973 Program) [2010CB126302]; National Natural Science Foundation of China [30925027, 30871402] ; National Basic Research Program of China (973 Program) [2010CB126302]; National Natural Science Foundation of China [30925027, 30871402] ; National Basic Research Program of China (973 Program) [2010CB126302]; National Natural Science Foundation of China [30925027, 30871402] ; National Basic Research Program of China (973 Program) [2010CB126302]; National Natural Science Foundation of China [30925027, 30871402]
Indexed BySCI
Language英语
WOS Research AreaReproductive Biology
WOS SubjectReproductive Biology
WOS IDWOS:000314228900002
WOS KeywordRAINBOW-TROUT ; TRANSGENIC ZEBRAFISH ; OOCYTE MATURATION ; GENE-EXPRESSION ; ACTIVIN ; APOPTOSIS ; ESTROGEN ; GONAD ; OVARY ; TRANSCRIPTOME
Funding OrganizationNational Basic Research Program of China (973 Program) [2010CB126302]; National Natural Science Foundation of China [30925027, 30871402] ; National Basic Research Program of China (973 Program) [2010CB126302]; National Natural Science Foundation of China [30925027, 30871402] ; National Basic Research Program of China (973 Program) [2010CB126302]; National Natural Science Foundation of China [30925027, 30871402] ; National Basic Research Program of China (973 Program) [2010CB126302]; National Natural Science Foundation of China [30925027, 30871402]
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Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19347
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorYin, Z (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Jiang, Nan,Jin, Xia,He, Jiangyan,et al. The Roles of follistatin 1 in Regulation of Zebrafish Fecundity and Sexual Differentiation[J]. BIOLOGY OF REPRODUCTION,2012,87(3).
APA Jiang, Nan,Jin, Xia,He, Jiangyan,Yin, Zhan,&Yin, Z .(2012).The Roles of follistatin 1 in Regulation of Zebrafish Fecundity and Sexual Differentiation.BIOLOGY OF REPRODUCTION,87(3).
MLA Jiang, Nan,et al."The Roles of follistatin 1 in Regulation of Zebrafish Fecundity and Sexual Differentiation".BIOLOGY OF REPRODUCTION 87.3(2012).
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