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Quantitative proteomic strategies for the identification of microRNA targets
Li, Chongyang1,2; Xiong, Qian1; Zhang, Jia3; Ge, Feng1; Bi, Li-Jun3; Ge, F (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
2012-10-01
Source PublicationEXPERT REVIEW OF PROTEOMICS
ISSN1478-9450
Volume9Issue:5Pages:549-559
AbstractMicroRNAs (miRNAs) are small noncoding RNAs, approximately 22 nucleotides in length, found in diverse organisms. They have emerged in recent years as key regulators of a broad spectrum of cellular functions. miRNAs regulate biological processes by inducing translational inhibition and degradation of their target mRNAs through base pairing to partially or fully complementary sites. In the field of miRNA research, the identification of the targets of individual miRNAs is of utmost importance. Our understanding of the molecular mechanisms by which individual miRNAs modulate cellular functions will remain incomplete until a full set of miRNA targets is identified and validated. Since a miRNA may regulate many of its targets at the translational level without affecting mRNA abundance, proteomic methods are best suited for revealing the full spectrum of miRNA targets. Quantitative proteomics is emerging as a powerful toolbox for identifying miRNA targets and for quantifying the contribution of translational repression by miRNAs. In this review, the authors summarize the quantitative proteomic approaches that have been employed for identification of miRNA targets and discuss current challenges as well as possible ways of overcoming them.; MicroRNAs (miRNAs) are small noncoding RNAs, approximately 22 nucleotides in length, found in diverse organisms. They have emerged in recent years as key regulators of a broad spectrum of cellular functions. miRNAs regulate biological processes by inducing translational inhibition and degradation of their target mRNAs through base pairing to partially or fully complementary sites. In the field of miRNA research, the identification of the targets of individual miRNAs is of utmost importance. Our understanding of the molecular mechanisms by which individual miRNAs modulate cellular functions will remain incomplete until a full set of miRNA targets is identified and validated. Since a miRNA may regulate many of its targets at the translational level without affecting mRNA abundance, proteomic methods are best suited for revealing the full spectrum of miRNA targets. Quantitative proteomics is emerging as a powerful toolbox for identifying miRNA targets and for quantifying the contribution of translational repression by miRNAs. In this review, the authors summarize the quantitative proteomic approaches that have been employed for identification of miRNA targets and discuss current challenges as well as possible ways of overcoming them.
SubtypeReview
Keyword2d Difference Gel Electrophoresis Isobaric Tags For Relative And Absolute Quantification Isotope-coded Affinity Tags Micrornas Pulsed Stable-isotope LabelIng By AmIno Acids In Cell Culture Quantitative Proteomics Stable-isotope LabelIng By AmIno Acids In Cell Culture
Department[Li, Chongyang ; Xiong, Qian ; Ge, Feng] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China ; [Li, Chongyang] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China ; [Zhang, Jia ; Bi, Li-Jun] Chinese Acad Sci, Inst Biophys, Key Lab Noncoding RNA, Beijing 100101, Peoples R China
DOI10.1586/EPR.12.49
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Basic Research Program of China (973 Program) [2012CB518700]; Hundred Talents Program of the Chinese Academy of Sciences ; National Basic Research Program of China (973 Program) [2012CB518700]; Hundred Talents Program of the Chinese Academy of Sciences ; National Basic Research Program of China (973 Program) [2012CB518700]; Hundred Talents Program of the Chinese Academy of Sciences ; National Basic Research Program of China (973 Program) [2012CB518700]; Hundred Talents Program of the Chinese Academy of Sciences
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods
WOS IDWOS:000312604600016
WOS KeywordLONG NONCODING RNAS ; GEL-ELECTROPHORESIS ; CANCER ; SILAC ; BIOGENESIS ; PROTEINS ; ELEGANS ; MIR-21 ; CELLS ; TRANSLATION
Funding OrganizationNational Basic Research Program of China (973 Program) [2012CB518700]; Hundred Talents Program of the Chinese Academy of Sciences ; National Basic Research Program of China (973 Program) [2012CB518700]; Hundred Talents Program of the Chinese Academy of Sciences ; National Basic Research Program of China (973 Program) [2012CB518700]; Hundred Talents Program of the Chinese Academy of Sciences ; National Basic Research Program of China (973 Program) [2012CB518700]; Hundred Talents Program of the Chinese Academy of Sciences
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/19346
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorGe, F (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Chinese Acad Sci, Inst Biophys, Key Lab Noncoding RNA, Beijing 100101, Peoples R China
Recommended Citation
GB/T 7714
Li, Chongyang,Xiong, Qian,Zhang, Jia,et al. Quantitative proteomic strategies for the identification of microRNA targets[J]. EXPERT REVIEW OF PROTEOMICS,2012,9(5):549-559.
APA Li, Chongyang,Xiong, Qian,Zhang, Jia,Ge, Feng,Bi, Li-Jun,&Ge, F .(2012).Quantitative proteomic strategies for the identification of microRNA targets.EXPERT REVIEW OF PROTEOMICS,9(5),549-559.
MLA Li, Chongyang,et al."Quantitative proteomic strategies for the identification of microRNA targets".EXPERT REVIEW OF PROTEOMICS 9.5(2012):549-559.
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