中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
题名: Quantitative proteomic strategies for the identification of microRNA targets
作者: Li, Chongyang1, 2; Xiong, Qian1; Zhang, Jia3; Ge, Feng1; Bi, Li-Jun3
通讯作者: Ge, F (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
关键词: 2D difference gel electrophoresis ; isobaric tags for relative and absolute quantification ; isotope-coded affinity tags ; microRNAs ; pulsed stable-isotope labeling by amino acids in cell culture ; quantitative proteomics ; stable-isotope labeling by amino acids in cell culture
刊名: EXPERT REVIEW OF PROTEOMICS
发表日期: 2012-10-01
DOI: 10.1586/EPR.12.49
卷: 9, 期:5, 页:549-559
收录类别: SCI
文章类型: Review
部门归属: [Li, Chongyang ; Xiong, Qian ; Ge, Feng] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China ; [Li, Chongyang] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China ; [Zhang, Jia ; Bi, Li-Jun] Chinese Acad Sci, Inst Biophys, Key Lab Noncoding RNA, Beijing 100101, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Basic Research Program of China (973 Program) [2012CB518700]; Hundred Talents Program of the Chinese Academy of Sciences
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
摘要: MicroRNAs (miRNAs) are small noncoding RNAs, approximately 22 nucleotides in length, found in diverse organisms. They have emerged in recent years as key regulators of a broad spectrum of cellular functions. miRNAs regulate biological processes by inducing translational inhibition and degradation of their target mRNAs through base pairing to partially or fully complementary sites. In the field of miRNA research, the identification of the targets of individual miRNAs is of utmost importance. Our understanding of the molecular mechanisms by which individual miRNAs modulate cellular functions will remain incomplete until a full set of miRNA targets is identified and validated. Since a miRNA may regulate many of its targets at the translational level without affecting mRNA abundance, proteomic methods are best suited for revealing the full spectrum of miRNA targets. Quantitative proteomics is emerging as a powerful toolbox for identifying miRNA targets and for quantifying the contribution of translational repression by miRNAs. In this review, the authors summarize the quantitative proteomic approaches that have been employed for identification of miRNA targets and discuss current challenges as well as possible ways of overcoming them.
英文摘要: MicroRNAs (miRNAs) are small noncoding RNAs, approximately 22 nucleotides in length, found in diverse organisms. They have emerged in recent years as key regulators of a broad spectrum of cellular functions. miRNAs regulate biological processes by inducing translational inhibition and degradation of their target mRNAs through base pairing to partially or fully complementary sites. In the field of miRNA research, the identification of the targets of individual miRNAs is of utmost importance. Our understanding of the molecular mechanisms by which individual miRNAs modulate cellular functions will remain incomplete until a full set of miRNA targets is identified and validated. Since a miRNA may regulate many of its targets at the translational level without affecting mRNA abundance, proteomic methods are best suited for revealing the full spectrum of miRNA targets. Quantitative proteomics is emerging as a powerful toolbox for identifying miRNA targets and for quantifying the contribution of translational repression by miRNAs. In this review, the authors summarize the quantitative proteomic approaches that have been employed for identification of miRNA targets and discuss current challenges as well as possible ways of overcoming them.
关键词[WOS]: LONG NONCODING RNAS ; GEL-ELECTROPHORESIS ; CANCER ; SILAC ; BIOGENESIS ; PROTEINS ; ELEGANS ; MIR-21 ; CELLS ; TRANSLATION
语种: 英语
WOS记录号: WOS:000312604600016
ISSN号: 1478-9450
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/19346
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Quantitative proteomic strategies for the identification of microRNA targets.pdf(4076KB)----开放获取View Download

作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Chinese Acad Sci, Inst Biophys, Key Lab Noncoding RNA, Beijing 100101, Peoples R China

Recommended Citation:
Li, CY; Xiong, Q; Zhang, J; Ge, F; Bi, LJ.Quantitative proteomic strategies for the identification of microRNA targets,EXPERT REVIEW OF PROTEOMICS,2012,9(5):549-559
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Li, Chongyang]'s Articles
[Xiong, Qian]'s Articles
[Zhang, Jia]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Li, Chongyang]‘s Articles
[Xiong, Qian]‘s Articles
[Zhang, Jia]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Quantitative proteomic strategies for the identification of microRNA targets.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2016  中国科学院水生生物研究所 - Feedback
Powered by CSpace